FastQCFastQC Report
Thu 26 May 2016
SRR937756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961665
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66510.691612983731341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52010.5408328264000457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35290.36696770704975223No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14790.1537957604779211No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14290.14859644470787645No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13170.1369499773829764No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11750.12218392059604956No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10010.10409030171629413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.10045078067726287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50250.032.269341
GTATCAA70500.028.868651
GTACATG56300.026.098881
TACATGG58000.025.1633762
GAGTACT47350.024.52504512-13
TAGGACA3800.023.7724444
TGCACGA1000.001645379423.7699662
ACATGGG58550.023.707833
ATCAACG85850.023.6995093
TCAACGC86750.023.4573064
TATCAAC87050.023.3194122
CAACGCA87650.023.2152335
GTACTTT49850.023.05631314-15
AACGCAG89600.022.748816
CATGGGG21150.022.2550544
GTGTAAG2858.185452E-1121.6905361
ATATAGG3850.020.9916572
TAGGGTG2751.2441888E-920.7457775
CATGGGA35700.020.642744
ACGCAGA99000.020.4907027
Produced by FastQC (version 0.11.5)