##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937756_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 961665 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.654638569564245 33.0 31.0 34.0 30.0 34.0 2 31.748145144099038 33.0 31.0 34.0 30.0 34.0 3 31.77946686216094 33.0 31.0 34.0 30.0 34.0 4 35.40512444562295 37.0 35.0 37.0 33.0 37.0 5 35.402259622633665 37.0 35.0 37.0 33.0 37.0 6 35.475668761990924 37.0 35.0 37.0 33.0 37.0 7 35.387384380215565 37.0 35.0 37.0 33.0 37.0 8 35.39801178164954 37.0 35.0 37.0 33.0 37.0 9 36.93773507406425 39.0 37.0 39.0 33.0 39.0 10-11 36.84037372681755 39.0 37.0 39.0 33.0 39.0 12-13 36.7249593153541 39.0 37.0 39.0 32.5 39.0 14-15 37.796741068875335 40.0 37.5 41.0 32.5 41.0 16-17 37.71763399936569 40.0 37.5 41.0 33.0 41.0 18-19 37.65732869554367 40.0 37.0 41.0 32.0 41.0 20-21 37.67929320501422 40.0 37.5 41.0 32.5 41.0 22-23 37.671282099275736 40.0 37.5 41.0 32.0 41.0 24-25 37.58647969927158 40.0 37.0 41.0 32.0 41.0 26-27 37.46517394310909 40.0 37.0 41.0 32.0 41.0 28-29 37.30859758855735 40.0 37.0 41.0 32.0 41.0 30-31 37.034319643534914 39.0 36.5 41.0 31.0 41.0 32-33 36.864831828131415 39.0 36.0 41.0 31.0 41.0 34-35 36.82154336489318 39.0 36.0 41.0 30.5 41.0 36-37 36.62965845694707 39.0 35.5 41.0 30.0 41.0 38-39 36.44334929523275 39.0 35.0 41.0 30.0 41.0 40-41 36.19116480271197 39.0 35.0 40.5 30.0 41.0 42-43 35.89295388726843 39.0 35.0 40.0 29.0 41.0 44-45 35.57869268404278 39.0 35.0 40.0 27.5 41.0 46-47 35.12924875086439 38.0 35.0 40.0 25.5 41.0 48-49 34.71148268887814 38.0 34.0 40.0 24.0 41.0 50-51 33.81051925566595 37.5 33.5 39.5 22.5 40.5 52-53 33.91925410615963 38.0 33.5 39.5 23.0 40.5 54-55 34.158073237561936 38.0 34.0 40.0 23.0 41.0 56-57 33.88205092209865 38.0 33.5 40.0 21.5 41.0 58-59 33.56927100393588 38.0 33.0 40.0 20.0 41.0 60-61 33.18570448129026 37.0 33.0 40.0 18.5 41.0 62-63 32.789288369650556 36.5 32.0 39.5 16.5 41.0 64-65 32.32084925623788 36.0 31.5 39.0 12.0 40.5 66-67 31.77940810989274 35.5 31.0 39.0 8.0 40.0 68-69 31.274591983694947 35.0 30.5 38.0 7.0 40.0 70-71 30.774772399952166 35.0 30.0 38.0 2.0 40.0 72-73 30.27242906833461 34.5 29.5 37.0 2.0 39.0 74-75 29.701977819718927 34.0 29.0 36.5 2.0 39.0 76-77 29.13266678105161 34.0 28.5 36.0 2.0 38.5 78-79 28.56784639141489 34.0 27.5 35.5 2.0 37.5 80-81 27.970089376238086 33.5 26.0 35.0 2.0 37.0 82-83 27.380825443371652 33.0 26.0 35.0 2.0 36.5 84-85 26.790315234515138 33.0 25.0 35.0 2.0 36.0 86-87 26.17195853025742 32.0 23.5 35.0 2.0 35.5 88-89 25.55914689626845 32.0 21.0 34.5 2.0 35.0 90-91 24.920705755122626 31.5 19.0 34.0 2.0 35.0 92-93 24.189937764190233 31.0 11.5 34.0 2.0 35.0 94-95 23.344884133248065 31.0 2.0 34.0 2.0 35.0 96-97 22.37806460669776 30.0 2.0 34.0 2.0 35.0 98-99 21.115592747994363 29.5 2.0 34.0 2.0 35.0 100-101 18.009463274633056 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.1281988916533905 1101 2 -0.1754403637520916 1101 3 -0.16022629874939653 1101 4 -0.09427090202608923 1101 5 -0.07707241293815059 1101 6 -0.01054090797602214 1101 7 -0.011671028148455775 1101 8 0.010085652731639527 1101 9 0.08595308897714915 1101 10-11 0.22891515938254514 1101 12-13 0.05687533079368734 1101 14-15 0.15024762396897273 1101 16-17 0.1508440883593707 1101 18-19 0.17529110900947842 1101 20-21 0.26913177351177353 1101 22-23 0.17957570043194693 1101 24-25 -0.11145409518641003 1101 26-27 -0.0433194493676794 1101 28-29 0.02307970383387925 1101 30-31 0.06633891072346643 1101 32-33 0.1378796063825618 1101 34-35 0.25409816281915454 1101 36-37 0.25527547112262994 1101 38-39 0.10653496585276656 1101 40-41 0.21763524509475474 1101 42-43 0.3629347563316614 1101 44-45 0.3412237573961434 1101 46-47 0.36962804283729156 1101 48-49 0.5126918624256049 1101 50-51 0.5688248076119109 1101 52-53 0.77488704184195 1101 54-55 0.6774873574671219 1101 56-57 0.7474465710045379 1101 58-59 0.5719253216607356 1101 60-61 0.6378238527290065 1101 62-63 0.6189665838533358 1101 64-65 0.5238653836907616 1101 66-67 0.7212185624091454 1101 68-69 0.6433082222422399 1101 70-71 0.6948344659575234 1101 72-73 0.830550394170892 1101 74-75 1.0057039960124534 1101 76-77 0.785276334081491 1101 78-79 1.0044808167099184 1101 80-81 1.299757995795055 1101 82-83 1.1506481739373022 1101 84-85 1.3674578819962235 1101 86-87 1.7663320597981702 1101 88-89 1.6609027628404078 1101 90-91 1.805156430454204 1101 92-93 1.8347863354606915 1101 94-95 1.9734221243199919 1101 96-97 2.2238798203894454 1101 98-99 2.7121805574819575 1101 100-101 3.1146329169704536 1102 1 -0.02724704046654125 1102 2 -0.204552739929877 1102 3 -0.4061708681749181 1102 4 -0.3312473457335514 1102 5 -0.2747742867154841 1102 6 -0.28101404056510404 1102 7 -0.1956713379606967 1102 8 -0.3217848739139768 1102 9 -0.11010173370814869 1102 10-11 -0.0032923467579522026 1102 12-13 -0.1485844646128811 1102 14-15 0.032299226484553856 1102 16-17 -0.0671898008757097 1102 18-19 -0.13708266303677874 1102 20-21 -0.12278922671237069 1102 22-23 -0.1335277146613052 1102 24-25 -0.27848132932584946 1102 26-27 0.03223601230537554 1102 28-29 -0.04043914131749915 1102 30-31 -0.07005006324643404 1102 32-33 -0.19369577715678332 1102 34-35 -0.039168173615827584 1102 36-37 -0.07629873888548389 1102 38-39 0.010785672025185988 1102 40-41 0.07386358610884258 1102 42-43 -0.029052841790210948 1102 44-45 0.09349952784873494 1102 46-47 0.13109901730242512 1102 48-49 0.2642597001590943 1102 50-51 -0.05170125123598979 1102 52-53 0.15307097878108067 1102 54-55 0.0598742965338559 1102 56-57 0.1876918272978898 1102 58-59 0.08009867334809684 1102 60-61 0.19891290145285012 1102 62-63 0.06763486060486201 1102 64-65 0.26035158472370057 1102 66-67 0.3159630201144914 1102 68-69 0.18700113728209544 1102 70-71 0.31803206866791456 1102 72-73 0.3964732180625816 1102 74-75 0.1253355306744588 1102 76-77 0.2193802080254592 1102 78-79 0.5474898564210378 1102 80-81 0.5966024289735969 1102 82-83 0.8136519562284796 1102 84-85 0.9365894336744311 1102 86-87 1.173766849601126 1102 88-89 0.9554183232778541 1102 90-91 1.1416785415430155 1102 92-93 1.4754000218861023 1102 94-95 1.5351023394047978 1102 96-97 1.4793778142377967 1102 98-99 1.7067485747657258 1102 100-101 1.9613753116078847 1103 1 0.05346465847850723 1103 2 -0.08452439793648736 1103 3 0.20162318000647161 1103 4 0.17658344206888898 1103 5 0.08023017452421755 1103 6 -0.021999712112886982 1103 7 0.06557885885870007 1103 8 0.013663611888780736 1103 9 0.16235929605094412 1103 10-11 0.1649095112171679 1103 12-13 0.23209311896975748 1103 14-15 0.33490511532096434 1103 16-17 0.3402525966469909 1103 18-19 0.3143186910806861 1103 20-21 0.27256321122391114 1103 22-23 0.2506217631112193 1103 24-25 0.094685626009273 1103 26-27 0.11328361635722928 1103 28-29 0.12859198239005565 1103 30-31 -0.040973277438432376 1103 32-33 0.15735130204334524 1103 34-35 0.049576381047479856 1103 36-37 0.008396584685606001 1103 38-39 0.28036789004858775 1103 40-41 0.16014921041997354 1103 42-43 0.05819850970299001 1103 44-45 0.0654934395686837 1103 46-47 -0.08382776588133112 1103 48-49 0.11582375831266489 1103 50-51 -0.15535684353117318 1103 52-53 0.09003103756446507 1103 54-55 0.10706371552299032 1103 56-57 0.020823092715410496 1103 58-59 0.186210571736801 1103 60-61 0.245291424520083 1103 62-63 0.5587412480504099 1103 64-65 0.2054494695712421 1103 66-67 0.4769297970000217 1103 68-69 0.6033397716178968 1103 70-71 0.7680845538408541 1103 72-73 0.48901245072935495 1103 74-75 0.4734329431554336 1103 76-77 0.7410933240701745 1103 78-79 0.7023447349178618 1103 80-81 0.987944999697369 1103 82-83 1.3129197880659866 1103 84-85 1.1742737878443776 1103 86-87 1.3734519385634947 1103 88-89 1.5026628516985738 1103 90-91 1.691048719467755 1103 92-93 1.945137071169679 1103 94-95 2.0291977574492 1103 96-97 1.7602915810986595 1103 98-99 1.9393339943846826 1103 100-101 2.5429608886823623 1104 1 0.18221811120950449 1104 2 0.036040513406288 1104 3 -0.04612697592331472 1104 4 0.13923157536658692 1104 5 0.06585790329549468 1104 6 0.11539670595714568 1104 7 0.07653849531953938 1104 8 0.1898642712490286 1104 9 0.23188616052568278 1104 10-11 0.20330387992428456 1104 12-13 0.09954834484518926 1104 14-15 0.29953578448250084 1104 16-17 0.2434558399865665 1104 18-19 0.38517318631368624 1104 20-21 0.5083424146454618 1104 22-23 0.33146467498861654 1104 24-25 0.2567545915265086 1104 26-27 0.21379595708608434 1104 28-29 0.31761898051889403 1104 30-31 0.253480077279562 1104 32-33 0.35305655019445936 1104 34-35 0.5652228213931991 1104 36-37 0.5698819272856284 1104 38-39 0.6198136710340734 1104 40-41 0.7262723504003787 1104 42-43 0.6520593312302125 1104 44-45 0.5857776772947787 1104 46-47 0.4381182378609907 1104 48-49 0.6466443945061968 1104 50-51 0.3151813369909746 1104 52-53 0.3563947549305908 1104 54-55 0.6458323157190193 1104 56-57 0.6096273340056158 1104 58-59 0.8117028643501882 1104 60-61 0.7153707259546351 1104 62-63 0.7459683709230802 1104 64-65 0.9632771448808697 1104 66-67 1.0304315345033608 1104 68-69 1.064927582363687 1104 70-71 1.039491486033672 1104 72-73 1.0508943005021436 1104 74-75 0.9762753204164163 1104 76-77 1.249918950016891 1104 78-79 1.3350053425985173 1104 80-81 1.6081312244144712 1104 82-83 1.727697476741028 1104 84-85 1.824942058360282 1104 86-87 2.0101266244763494 1104 88-89 1.9411374128676044 1104 90-91 2.139979486642307 1104 92-93 1.9314865320981305 1104 94-95 2.266977317281686 1104 96-97 2.494556038017482 1104 98-99 2.778891153789477 1104 100-101 3.1341798141033728 1105 1 0.14945385078023676 1105 2 0.16544538733327485 1105 3 0.0854005591396394 1105 4 0.1858712286737969 1105 5 0.04121109336840334 1105 6 0.08979162590926393 1105 7 0.10664494843983618 1105 8 0.3096774821900752 1105 9 0.23138366082974215 1105 10-11 0.30956688250399367 1105 12-13 0.23826546632209755 1105 14-15 0.3437746414717253 1105 16-17 0.3521945337434005 1105 18-19 0.33641151199749686 1105 20-21 0.23763814691874074 1105 22-23 0.3285745015782098 1105 24-25 0.2501209554131094 1105 26-27 0.3299710062158354 1105 28-29 0.42908269491983475 1105 30-31 0.3369465570291439 1105 32-33 0.38557692223627527 1105 34-35 0.481026276798751 1105 36-37 0.40703201786359955 1105 38-39 0.43650306220927604 1105 40-41 0.5545115530581839 1105 42-43 0.4571284969526843 1105 44-45 0.7581533166216161 1105 46-47 0.6912987088664337 1105 48-49 0.6384667720659039 1105 50-51 0.5532288856246268 1105 52-53 0.5423714927153682 1105 54-55 0.582075026272797 1105 56-57 0.7227720219117018 1105 58-59 0.6878178055545234 1105 60-61 0.8383724094886489 1105 62-63 0.9282243705133979 1105 64-65 0.7790076258082834 1105 66-67 0.9032507818420363 1105 68-69 0.7541318404884976 1105 70-71 0.8062240857814622 1105 72-73 0.7775779945470376 1105 74-75 0.7043807365309718 1105 76-77 0.87421721079183 1105 78-79 1.2718974619950068 1105 80-81 1.2591688702834105 1105 82-83 1.2698943285964361 1105 84-85 1.5117653641041429 1105 86-87 1.6663048102284037 1105 88-89 1.646479272692595 1105 90-91 1.5913154316938076 1105 92-93 1.6319928725560757 1105 94-95 1.416269016754864 1105 96-97 1.6472394504065022 1105 98-99 1.8374458110334722 1105 100-101 2.4649375964473883 1106 1 -0.03910677768834603 1106 2 -0.09618280695111991 1106 3 0.10617070387391081 1106 4 0.06586045721844158 1106 5 -0.13174524857660685 1106 6 -0.06503586966290698 1106 7 -0.0911158206879108 1106 8 -0.07668473807616039 1106 9 -0.014464781069214894 1106 10-11 -0.21428286715276812 1106 12-13 -0.29166399640143936 1106 14-15 -0.5350121087787585 1106 16-17 -0.38012369973792204 1106 18-19 0.0970101871944351 1106 20-21 -0.0791808646999641 1106 22-23 -0.03020054617356749 1106 24-25 -0.39308640298056474 1106 26-27 -0.28842440502959477 1106 28-29 -0.10786862512058804 1106 30-31 0.028405953154425845 1106 32-33 0.01178235480259815 1106 34-35 0.1272262233711885 1106 36-37 -0.1556655155160982 1106 38-39 -0.3937559182409629 1106 40-41 -0.25517745697774785 1106 42-43 0.040085175505673476 1106 44-45 0.027177934123940872 1106 46-47 0.25963406781587395 1106 48-49 0.16224239634837545 1106 50-51 0.020279261515469216 1106 52-53 0.20400025994951676 1106 54-55 0.17813900470696353 1106 56-57 0.0023342596528976856 1106 58-59 -0.22127547997570218 1106 60-61 0.32567011346413466 1106 62-63 0.20673166452184688 1106 64-65 0.4129285429862435 1106 66-67 0.42750052204513267 1106 68-69 0.32445211223856774 1106 70-71 0.4336509756025073 1106 72-73 0.662923587095257 1106 74-75 0.651885289691041 1106 76-77 0.9872354886051369 1106 78-79 1.1368976088133813 1106 80-81 1.1961042909681865 1106 82-83 1.053284586875023 1106 84-85 0.9376177880038838 1106 86-87 1.3923112859929851 1106 88-89 1.4627722448193055 1106 90-91 1.3211062405771017 1106 92-93 1.4838420320745982 1106 94-95 1.3418786531967584 1106 96-97 1.6672343225062143 1106 98-99 1.711890676436834 1106 100-101 2.0956181246784134 1107 1 -0.08770834476345968 1107 2 0.007576987268343771 1107 3 0.05941864125020757 1107 4 -0.0607286711052879 1107 5 -0.07349808954887038 1107 6 0.1379767099324951 1107 7 0.1179219180983111 1107 8 -0.0652341187330876 1107 9 -0.03831857014416329 1107 10-11 0.029660469071572493 1107 12-13 0.028379591118302017 1107 14-15 0.18478722554789329 1107 16-17 0.0010984557687123697 1107 18-19 0.19305432326459027 1107 20-21 0.13016710158642297 1107 22-23 0.015225358749297868 1107 24-25 0.17299390776582868 1107 26-27 0.22435051880344048 1107 28-29 0.028680399719853256 1107 30-31 -2.8656951034378153E-4 1107 32-33 0.04080252514973637 1107 34-35 0.2599654172985879 1107 36-37 0.07792207834903309 1107 38-39 0.27136405863097224 1107 40-41 0.16501216355879933 1107 42-43 0.17334734554147957 1107 44-45 0.18258745666270215 1107 46-47 0.20787574133942854 1107 48-49 -0.11637488595494005 1107 50-51 -0.1318967786917895 1107 52-53 0.02206758339411863 1107 54-55 0.2015965772972308 1107 56-57 0.21998312808234033 1107 58-59 0.15327511638755453 1107 60-61 0.4838561165330546 1107 62-63 0.3102001339920548 1107 64-65 0.26346597413947137 1107 66-67 0.21125045718275004 1107 68-69 0.18584787653979618 1107 70-71 0.41042171859180954 1107 72-73 0.43298533608155054 1107 74-75 0.4537748234973158 1107 76-77 0.33593564649870444 1107 78-79 0.47344700429599484 1107 80-81 0.6104766825738821 1107 82-83 0.8715257414995286 1107 84-85 1.0210760277500697 1107 86-87 1.2784033090321039 1107 88-89 1.1818847791963663 1107 90-91 1.3992420586955774 1107 92-93 1.7750663373058444 1107 94-95 1.8801556118553293 1107 96-97 1.8212405937718081 1107 98-99 2.248956747110885 1107 100-101 3.0195008238429857 1108 1 -0.006543644060570841 1108 2 0.008468122972978165 1108 3 0.011452161312149656 1108 4 -0.1225236947120365 1108 5 -0.005897779852645613 1108 6 -0.01186312853966598 1108 7 0.03998321973332253 1108 8 -0.1131869356024211 1108 9 0.007086843467284609 1108 10-11 0.04354442407453263 1108 12-13 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-0.06983386225219945 2316 80-81 -0.4513706560061337 2316 82-83 -0.13782942478633586 2316 84-85 -0.054484605236307715 2316 86-87 -0.3511981126485253 2316 88-89 -0.7612649203889781 2316 90-91 -0.7430698648044682 2316 92-93 -0.9112796049806953 2316 94-95 -0.5333757412799862 2316 96-97 -0.49833290203931213 2316 98-99 -0.8147269289490318 2316 100-101 -1.2117676876235421 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4159.0 3 885.0 4 834.0 5 923.0 6 1106.0 7 1394.0 8 1613.0 9 2037.0 10 2437.0 11 3276.0 12 4229.0 13 5377.0 14 6138.0 15 7638.0 16 9709.0 17 12777.0 18 11185.0 19 8053.0 20 8138.0 21 8502.0 22 9650.0 23 10624.0 24 11767.0 25 13598.0 26 15408.0 27 17909.0 28 21008.0 29 24412.0 30 29732.0 31 36174.0 32 44451.0 33 57351.0 34 75119.0 35 101957.0 36 134769.0 37 158085.0 38 92621.0 39 6620.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79670448947557 19.046142991926125 14.851923285658225 22.30522923294008 2 14.929299503907407 20.276793798216737 41.12384373709179 23.67006296078407 3 19.033794856655856 25.498807160583205 29.72251227524079 25.744885707520154 4 12.36164959680069 16.64397986037444 37.21355951296903 33.78081102985584 5 13.210026953278204 37.95812228276743 33.74594240510026 15.08590835885411 6 31.009127841898028 36.314907418794725 17.496524166164658 15.17944057314259 7 28.27886406007547 33.53921923148668 20.448669383790836 17.73324732464701 8 26.536284901353845 34.521047639892025 19.478368716714087 19.464298742040043 9 26.978098599035725 16.99437821657936 20.486783493593418 35.54073969079149 10-11 25.015854752230737 26.926606436847017 26.583820700860823 21.473718110061423 12-13 26.065186939265583 24.5495885168262 27.493808991762 21.891415552146213 14-15 22.98120203977201 25.59224986686037 26.418236637315157 25.00831145605246 16-17 21.472368983232773 29.200873271433185 26.9049405487646 22.421817196569442 18-19 21.45144399347689 27.07330162087812 30.141872424231376 21.333381961413618 20-21 22.366818278914437 26.03645988006481 30.13978545043426 21.456936390586495 22-23 22.035807646836 25.91133071661667 30.384498949822298 21.668362686725036 24-25 21.749200075237997 26.269680285404196 30.37999878076929 21.601120858588516 26-27 21.443314332939224 26.440753213904767 30.45042866827542 21.665503784880592 28-29 21.17261110647878 26.483782206287 30.764456349537227 21.57915033769699 30-31 21.370919618404354 26.259169213851475 30.623180814942792 21.746730352801382 32-33 21.17588874099846 26.541948825278634 30.360822539474412 21.921339894248497 34-35 21.761206288258034 26.46676860123468 30.086592269023875 21.68543284148341 36-37 21.75772025951993 26.079023111279696 30.206029899397468 21.9572267298029 38-39 21.42532632967555 26.60799488167521 30.260584418493906 21.70609437015533 40-41 21.420418284922416 26.579560876371772 30.345583887512667 21.654436951193144 42-43 21.42345795181363 26.52772003096966 29.854509304964626 22.194312712252085 44-45 21.406624417149974 26.691600238220165 29.722446414791744 22.179328929838118 46-47 21.724469632859623 26.938044280288974 29.220738616230545 22.116747470620847 48-49 21.8068881631096 27.03713721241343 28.86723711042563 22.288737514051334 50-51 21.722302677897225 27.153972706678875 28.83188608657633 22.29183852884757 52-53 21.885329894682634 27.429841414127726 28.48212898901376 22.202699702175877 54-55 21.71839972319176 27.209437326799435 28.588997080798617 22.48316586921019 56-57 21.83806074219859 27.507252733742753 28.359400110751025 22.29528641330763 58-59 22.026606554866774 27.059372555310528 28.537306156633047 22.37671473318965 60-61 21.933586302174824 27.198630550884452 28.87949806314219 21.988285083798534 62-63 21.741284228763178 27.3145126465868 29.0016965600513 21.942506564598723 64-65 21.768411927914073 27.27331451689364 28.801030215701413 22.157243339490872 66-67 21.83839544652053 27.464814045938006 28.333803052427424 22.36298745511404 68-69 21.56866830829306 27.296562221790428 28.690729151961225 22.444040317955285 70-71 21.809092817629477 27.254124941459253 28.665041677888603 22.271740563022664 72-73 21.801251329192105 27.25780309357252 28.44432033152044 22.496625245714934 74-75 21.551138435888085 27.92158599489397 28.21580784661074 22.3114677226072 76-77 21.67205939820242 27.46088315748339 28.45929399505015 22.407763449264035 78-79 21.743500182095456 27.346934309649807 28.458628380944738 22.450937127309995 80-81 21.66060750044545 27.868517655983155 28.106299553090903 22.36457529048049 82-83 21.885905302578436 27.561676575585874 28.10974494843073 22.442673173404955 84-85 21.71527960819327 27.40615568295826 28.059583627212692 22.818981081635776 86-87 21.678608378195065 27.39411607197742 28.286540647488383 22.64073490233913 88-89 21.834216747957484 27.23697358508653 28.052000420954798 22.87680924600119 90-91 21.617463723607045 27.226218924834033 28.444557668433273 22.71175968312565 92-93 21.816254343330154 27.052265824829004 28.454790295945532 22.67668953589531 94-95 21.57906883095166 27.027480390313123 28.31135030608146 23.082100472653753 96-97 21.632373642701666 26.97596900759493 28.291927116166292 23.09973023353711 98-99 21.574716919399524 27.01251108408903 28.135038850318168 23.27773314619327 100-101 21.83199760809165 26.07654106717033 28.98402234392433 23.107438980813686 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1775.0 1 1079.5 2 508.5 3 633.0 4 510.0 5 474.0 6 847.5 7 1788.5 8 2817.0 9 3165.5 10 2627.5 11 1919.0 12 1822.0 13 1903.0 14 1938.0 15 1952.0 16 1890.5 17 1827.0 18 1897.0 19 2015.0 20 2077.5 21 2273.0 22 2589.0 23 3057.0 24 3576.5 25 4229.0 26 5317.0 27 6476.0 28 7074.5 29 8032.0 30 9831.0 31 11698.5 32 14105.5 33 16933.5 34 19447.0 35 21356.5 36 24286.0 37 27091.0 38 28225.0 39 28508.5 40 28485.0 41 29651.5 42 30347.0 43 31525.0 44 33715.0 45 35045.5 46 35088.0 47 35902.0 48 39486.0 49 39172.0 50 35599.0 51 32549.5 52 29871.5 53 29407.5 54 29046.5 55 27259.5 56 24674.5 57 22213.5 58 20252.0 59 17953.0 60 15387.5 61 13113.5 62 10878.5 63 8481.5 64 6561.0 65 5242.5 66 3934.5 67 3125.0 68 2431.5 69 1675.0 70 1233.0 71 913.5 72 705.0 73 581.5 74 459.5 75 358.5 76 237.5 77 133.0 78 82.0 79 58.0 80 41.5 81 30.0 82 20.0 83 13.5 84 12.5 85 8.0 86 3.0 87 4.0 88 2.0 89 1.5 90 2.5 91 1.5 92 0.5 93 0.0 94 1.5 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36530392600333794 2 0.3091513156868556 3 0.31487056303390476 4 0.3075915209558422 5 0.308735370425252 6 0.22700212652014992 7 0.2154596455106508 8 0.2261702359969428 9 0.22700212652014992 10-11 0.2260142565238415 12-13 0.2297057707205732 14-15 0.22315463285031692 16-17 0.2136918781488356 18-19 0.20797263080178646 20-21 0.2058929044937686 22-23 0.21478373446054497 24-25 0.21249603552172536 26-27 0.22081494075379682 28-29 0.23136955176698745 30-31 0.2363089017485299 32-33 0.23355326439040625 34-35 0.23147353808238838 36-37 0.21285998762562847 38-39 0.20547695923216505 40-41 0.19887382820420832 42-43 0.20963641184820078 44-45 0.21301596709872983 46-47 0.22263470127331245 48-49 0.23256539439409774 50-51 0.22871790072426468 52-53 0.24686351276172058 54-55 0.22419449600432584 56-57 0.19284262191095652 58-59 0.17428106461189707 60-61 0.1937265055918641 62-63 0.1852516208866913 64-65 0.19403846453806678 66-67 0.19461038927277172 68-69 0.19180275875694758 70-71 0.19377849874956454 72-73 0.20438510292045564 74-75 0.20953242553279988 76-77 0.2121320834178222 78-79 0.20833658290568963 80-81 0.20402115081655253 82-83 0.1912828271799431 84-85 0.1875913129832114 86-87 0.193830491907265 88-89 0.20194142450853467 90-91 0.199185787150411 92-93 0.19341454664566143 94-95 0.19461038927277172 96-97 0.20235736977013827 98-99 0.2033972329241472 100-101 0.18322388773637388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 961665.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.47511180139261 #Duplication Level Percentage of deduplicated Percentage of total 1 76.85782332070328 40.33122871565585 2 12.558306487826265 13.179970739696747 3 4.2997482825720015 6.768893155374349 4 1.9391255499852555 4.070233201296529 5 1.030486939875237 2.7037458689914002 6 0.6560046133450385 2.0654349256506133 7 0.44253034944164843 1.625528069372189 8 0.3077920498148209 1.2921137780490024 9 0.23860961570548478 1.1268959634929374 >10 1.4908790852822782 14.895888284363663 >50 0.12330248779351814 4.470783767356375 >100 0.05241319526194364 4.7202496671315055 >500 0.0013897437835303488 0.47975521816555594 >1k 0.0011912089573117277 1.034521024187616 >5k 3.970696524372426E-4 1.234757621215672 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6651 0.691612983731341 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5201 0.5408328264000457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3529 0.36696770704975223 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1479 0.1537957604779211 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1429 0.14859644470787645 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1317 0.1369499773829764 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1175 0.12218392059604956 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1001 0.10409030171629413 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 966 0.10045078067726287 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 5.199315770044662E-5 0.0 0.0 0.0 0.0 18-19 1.0398631540089325E-4 0.0 0.0 0.0 0.0 20-21 1.0398631540089325E-4 0.0 0.0 1.0398631540089325E-4 0.0 22-23 1.0398631540089325E-4 0.0 0.0 1.0398631540089325E-4 0.0 24-25 1.0398631540089325E-4 0.0 0.0 1.5597947310133986E-4 0.0 26-27 1.0398631540089325E-4 0.0 0.0 9.358768386080391E-4 0.0 28-29 1.0398631540089325E-4 0.0 0.0 0.0020797263080178647 0.0 30-31 1.0398631540089325E-4 0.0 0.0 0.0049913431392428756 0.0 32-33 1.0398631540089325E-4 0.0 0.0 0.008578871020573692 5.199315770044662E-5 34-35 1.0398631540089325E-4 0.0 0.0 0.01185443995570183 1.0398631540089325E-4 36-37 1.0398631540089325E-4 0.0 0.0 0.013518221002116122 1.0398631540089325E-4 38-39 1.0398631540089325E-4 0.0 0.0 0.015026022575429074 1.0398631540089325E-4 40-41 1.0398631540089325E-4 0.0 0.0 0.016377844675640685 1.0398631540089325E-4 42-43 2.079726308017865E-4 0.0 0.0 0.019185475191464803 1.0398631540089325E-4 44-45 2.079726308017865E-4 0.0 0.0 0.021941112549588474 1.0398631540089325E-4 46-47 2.079726308017865E-4 0.0 0.0 0.025216681484716612 1.0398631540089325E-4 48-49 2.079726308017865E-4 0.0 0.0 0.029636099889254575 1.0398631540089325E-4 50-51 2.079726308017865E-4 0.0 0.0 0.03587527881330817 1.0398631540089325E-4 52-53 2.079726308017865E-4 0.0 0.0 0.05058934244253456 1.0398631540089325E-4 54-55 2.079726308017865E-4 0.0 0.0 0.07013876973790249 1.0398631540089325E-4 56-57 2.079726308017865E-4 0.0 0.0 0.0923398480759932 1.0398631540089325E-4 58-59 2.079726308017865E-4 0.0 0.0 0.12036416007653393 1.0398631540089325E-4 60-61 2.079726308017865E-4 0.0 0.0 0.16175071360608945 1.0398631540089325E-4 62-63 2.079726308017865E-4 0.0 0.0 0.19164677928384624 1.0398631540089325E-4 64-65 2.079726308017865E-4 0.0 0.0 0.22097092022689813 1.0398631540089325E-4 66-67 2.079726308017865E-4 0.0 0.0 0.281490955790218 1.0398631540089325E-4 68-69 2.079726308017865E-4 0.0 0.0 0.3586488018176808 1.0398631540089325E-4 70-71 2.079726308017865E-4 0.0 0.0 0.42504406420115115 1.0398631540089325E-4 72-73 2.079726308017865E-4 0.0 0.0 0.4892556139612027 1.0398631540089325E-4 74-75 2.079726308017865E-4 0.0 0.0 0.6065521777334103 1.0398631540089325E-4 76-77 2.079726308017865E-4 0.0 0.0 0.6941606484586629 1.0398631540089325E-4 78-79 2.079726308017865E-4 0.0 0.0 0.7686668434434029 1.0398631540089325E-4 80-81 2.079726308017865E-4 0.0 0.0 0.8657900620278371 1.0398631540089325E-4 82-83 2.079726308017865E-4 0.0 0.0 1.0268648645838208 1.0398631540089325E-4 84-85 2.079726308017865E-4 0.0 0.0 1.1871597697742977 1.0398631540089325E-4 86-87 2.079726308017865E-4 0.0 0.0 1.3965362158339962 1.0398631540089325E-4 88-89 2.079726308017865E-4 0.0 0.0 1.6655488137761072 1.0398631540089325E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5025 0.0 32.26934 1 GTATCAA 7050 0.0 28.86865 1 GTACATG 5630 0.0 26.09888 1 TACATGG 5800 0.0 25.163376 2 GAGTACT 4735 0.0 24.525045 12-13 TAGGACA 380 0.0 23.772444 4 TGCACGA 100 0.0016453794 23.769966 2 ACATGGG 5855 0.0 23.70783 3 ATCAACG 8585 0.0 23.699509 3 TCAACGC 8675 0.0 23.457306 4 TATCAAC 8705 0.0 23.319412 2 CAACGCA 8765 0.0 23.215233 5 GTACTTT 4985 0.0 23.056313 14-15 AACGCAG 8960 0.0 22.74881 6 CATGGGG 2115 0.0 22.255054 4 GTGTAAG 285 8.185452E-11 21.690536 1 ATATAGG 385 0.0 20.991657 2 TAGGGTG 275 1.2441888E-9 20.745777 5 CATGGGA 3570 0.0 20.64274 4 ACGCAGA 9900 0.0 20.490702 7 >>END_MODULE