FastQCFastQC Report
Thu 26 May 2016
SRR937756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961665
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68480.7120982878653169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57940.6024967114327755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.4177130289653882No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19700.20485304133975968No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15750.16377844675640685No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.15140407522370056No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.11157731642515846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85000.025.939991
GGTATCA63300.024.9232751
GAGTACT54350.022.98628612-13
AGTACTT56350.022.21259712-13
CGATACT1503.0303074E-522.1687894
GTACTTT56650.022.09266914-15
GTACATG55950.021.7425581
GTAAGGT3301.8189894E-1221.5929784
GAGTGCA4650.021.452559
ATCAACG102950.021.3643593
TCAACGC103150.021.276884
AATTCGC1900.021.2487278-79
TAAGGTG4050.021.1131345
CAACGCA104400.021.067635
TATCAAC104800.021.0325492
AACGCAG106400.020.6683986
ACATGGG55250.020.635463
TACATGG57450.020.589442
CAATTCG1850.020.53930578-79
TACTTTT60900.020.4339114-15
Produced by FastQC (version 0.11.5)