FastQCFastQC Report
Thu 26 May 2016
SRR937755_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937755_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862630
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.41558953433105733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27470.3184447561526958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.21167823980153716No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11730.13597950453844637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.043.4581071
GTATCAA33950.035.7177541
CTAGCGC550.002578957734.54638
ATCAACG42350.028.402063
CAACGCA43100.028.0230225
TCAACGC42950.028.0085474
TATCAAC43250.027.920962
AACGCAG44500.027.004716
GTACATG32650.025.0512281
TACATGG31850.024.9281352
GTGTAGC6450.023.5925031
ACGCAGA51550.023.3128877
CGCAGAG51550.023.036458
GTAGCCC5600.022.9223163
ACATGGG33450.022.8829773
GTATATG4000.022.5879761
CATGGGG15250.022.1371524
GAGTACT32700.021.28110112-13
GCAGAGT55250.020.8919149
GTACTTT33850.020.41778214-15
Produced by FastQC (version 0.11.5)