##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937755_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 862630 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64494395047703 33.0 31.0 34.0 30.0 34.0 2 31.793480402953758 33.0 31.0 34.0 30.0 34.0 3 31.811668965837033 33.0 31.0 34.0 30.0 34.0 4 35.407102697564426 37.0 35.0 37.0 33.0 37.0 5 35.39025654104309 37.0 35.0 37.0 33.0 37.0 6 35.44778178361522 37.0 35.0 37.0 33.0 37.0 7 35.37987201928985 37.0 35.0 37.0 33.0 37.0 8 35.375942176831316 37.0 35.0 37.0 33.0 37.0 9 37.00833613484345 39.0 37.0 39.0 33.0 39.0 10-11 36.86442159442635 39.0 37.0 39.0 33.0 39.0 12-13 36.746226076069696 39.0 37.0 39.0 33.0 39.0 14-15 37.895858015603444 40.0 38.0 41.0 33.0 41.0 16-17 37.80846828883763 40.0 38.0 41.0 33.0 41.0 18-19 37.73416934259184 40.0 38.0 41.0 32.0 41.0 20-21 37.64530157773321 40.0 37.0 41.0 32.0 41.0 22-23 37.55561306701598 40.0 37.0 41.0 32.0 41.0 24-25 37.58265826600049 40.0 37.0 41.0 32.0 41.0 26-27 37.492305507575665 40.0 37.0 41.0 32.0 41.0 28-29 37.35897429952587 40.0 37.0 41.0 32.0 41.0 30-31 37.11105862304812 39.5 36.5 41.0 31.0 41.0 32-33 36.975587447689044 39.0 36.0 41.0 31.0 41.0 34-35 36.98768591400716 40.0 36.0 41.0 31.0 41.0 36-37 36.84755457148488 39.5 36.0 41.0 30.5 41.0 38-39 36.70002434415682 39.0 36.0 41.0 30.0 41.0 40-41 36.46487485944148 39.0 35.5 41.0 30.0 41.0 42-43 36.193209719114805 39.0 35.0 41.0 30.0 41.0 44-45 35.94621216512294 39.0 35.0 41.0 28.5 41.0 46-47 35.60341977441081 39.0 35.0 40.5 27.5 41.0 48-49 35.283985602170105 39.0 35.0 40.0 26.0 41.0 50-51 34.39958614933401 38.0 34.0 39.5 24.5 40.5 52-53 34.4842081773182 38.0 34.0 39.5 24.0 40.5 54-55 34.773372709040956 38.0 34.0 40.0 24.0 41.0 56-57 34.54411682876784 38.0 34.0 40.0 23.5 41.0 58-59 34.30279320218402 38.0 34.0 40.0 23.0 41.0 60-61 33.975203737407696 38.0 33.5 40.0 22.5 41.0 62-63 33.590616486790395 37.5 33.0 40.0 21.5 41.0 64-65 33.19858861852706 37.0 32.5 40.0 19.5 41.0 66-67 32.751246768602996 36.0 32.0 39.0 18.0 41.0 68-69 32.25063120920905 36.0 31.0 39.0 14.5 40.0 70-71 31.790851234016902 35.0 31.0 38.5 10.5 40.0 72-73 31.319278253712483 35.0 31.0 38.0 7.5 40.0 74-75 30.78326049407046 35.0 30.0 37.0 6.5 39.0 76-77 30.28937725328356 34.5 29.5 36.5 2.0 39.0 78-79 29.722103914772266 34.0 29.0 36.0 2.0 38.5 80-81 29.148949143897152 34.0 29.0 35.5 2.0 37.5 82-83 28.629414117292466 34.0 28.0 35.0 2.0 37.0 84-85 28.102534690423475 33.5 26.5 35.0 2.0 36.5 86-87 27.548174767861077 33.0 26.0 35.0 2.0 36.0 88-89 27.082173121732374 33.0 25.5 35.0 2.0 36.0 90-91 26.51767849483556 32.5 25.0 35.0 2.0 35.0 92-93 25.874444431563937 32.0 23.5 34.5 2.0 35.0 94-95 25.120605589882103 32.0 19.5 34.0 2.0 35.0 96-97 24.265380870129718 31.0 12.0 34.0 2.0 35.0 98-99 23.211536811842855 31.0 2.0 34.0 2.0 35.0 100-101 20.45332181816074 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06117031690690311 1101 2 0.1134100566939118 1101 3 0.16761786879367335 1101 4 0.13400287275165823 1101 5 0.19942769949246753 1101 6 0.12301924306598977 1101 7 0.13343367413399676 1101 8 0.14075633742069726 1101 9 0.2352908144915915 1101 10-11 0.20252373821993785 1101 12-13 0.10406477928192714 1101 14-15 0.24956484915263388 1101 16-17 0.49668971971188824 1101 18-19 0.3964783864537509 1101 20-21 0.2785338528380876 1101 22-23 0.24160231073305027 1101 24-25 0.13614026296943393 1101 26-27 0.13276940686060357 1101 28-29 0.18117415442743123 1101 30-31 0.2675247009547377 1101 32-33 0.18025568734864095 1101 34-35 0.21914435078252836 1101 36-37 0.2508543786039823 1101 38-39 0.3190186317120194 1101 40-41 0.2292936042217164 1101 42-43 0.3903717965059812 1101 44-45 0.3290344212725955 1101 46-47 0.3718496332655903 1101 48-49 0.6618369198602565 1101 50-51 0.4011853637963796 1101 52-53 0.4198178750801702 1101 54-55 0.5228258877271301 1101 56-57 0.34951649820291664 1101 58-59 0.3967630682219365 1101 60-61 0.45735051133173243 1101 62-63 0.4618350695702702 1101 64-65 0.6925584801331226 1101 66-67 0.5829038966419873 1101 68-69 0.4186635416230118 1101 70-71 0.5492298507875653 1101 72-73 0.5185721596337594 1101 74-75 0.4220071240173091 1101 76-77 0.5074631490369832 1101 78-79 0.564239184908427 1101 80-81 0.47145354576626985 1101 82-83 0.6128955955315085 1101 84-85 0.7634784164042472 1101 86-87 0.7591746532472676 1101 88-89 0.6584347925476983 1101 90-91 0.538332364094785 1101 92-93 0.34262898025263056 1101 94-95 0.33292391678146416 1101 96-97 0.40209266709411295 1101 98-99 0.44239974816765226 1101 100-101 0.6533872612781018 1102 1 0.05887983654480777 1102 2 0.13101390397088508 1102 3 0.055646230598313196 1102 4 -0.008350938023632182 1102 5 -0.035836572664052824 1102 6 0.0468949913504062 1102 7 -0.02051306991622681 1102 8 -0.13169493283773193 1102 9 0.02148496692260693 1102 10-11 -0.03276517256995959 1102 12-13 -0.042662388917804606 1102 14-15 0.07957744223190844 1102 16-17 0.1398214725225202 1102 18-19 -0.07375358171677959 1102 20-21 0.11701437919230528 1102 22-23 0.1889965471179238 1102 24-25 0.15682028925063918 1102 26-27 0.10026785553924356 1102 28-29 -0.025896220809826787 1102 30-31 0.07800296420484898 1102 32-33 0.03435071947011892 1102 34-35 -0.021388482477796344 1102 36-37 0.22910850329371613 1102 38-39 0.13091316011033172 1102 40-41 0.07961253764265308 1102 42-43 0.11045958304415393 1102 44-45 0.00366621058490324 1102 46-47 0.004666011568993156 1102 48-49 0.15753656579396846 1102 50-51 0.10528313354379293 1102 52-53 0.2073197001641205 1102 54-55 -0.0026801167990768704 1102 56-57 0.013976638369364025 1102 58-59 -0.025271170353043715 1102 60-61 -0.057323163657308385 1102 62-63 -9.240923511768528E-4 1102 64-65 -0.10668319748405253 1102 66-67 -0.09096865774551333 1102 68-69 -0.025571930708714774 1102 70-71 0.07032325858432031 1102 72-73 -0.03347193439456575 1102 74-75 -0.128500249321867 1102 76-77 -0.14388558800492035 1102 78-79 0.17147381770153558 1102 80-81 -0.039631101627509935 1102 82-83 0.0957416314491617 1102 84-85 0.26863610365785817 1102 86-87 0.12989756900139326 1102 88-89 0.04447472538461028 1102 90-91 0.057804184798534664 1102 92-93 0.17497448689593398 1102 94-95 0.5086580568019023 1102 96-97 0.7483466822788145 1102 98-99 0.547710742473388 1102 100-101 0.8381952624461562 1103 1 0.10093118089206854 1103 2 0.14475499564564842 1103 3 0.059551721366052135 1103 4 0.05394862465858097 1103 5 -0.07374554577710057 1103 6 0.16638802665977437 1103 7 0.08623093721043062 1103 8 0.024178487959154893 1103 9 0.1264230135698341 1103 10-11 0.1364869330341847 1103 12-13 0.11780590554283776 1103 14-15 0.20214182512792433 1103 16-17 0.13014682885552276 1103 18-19 0.1453041604276919 1103 20-21 -0.014568670694323771 1103 22-23 -0.04773713444993888 1103 24-25 0.08768763618942188 1103 26-27 0.17594711533840268 1103 28-29 0.009996231409154177 1103 30-31 -0.05844322141225433 1103 32-33 0.10427196177655418 1103 34-35 0.3130513053420856 1103 36-37 0.051367168664626206 1103 38-39 0.1986781827860682 1103 40-41 0.12478078351243482 1103 42-43 0.27801100837619686 1103 44-45 0.26873848949646373 1103 46-47 0.22363568277081214 1103 48-49 0.2007166354408696 1103 50-51 0.06558945845470987 1103 52-53 0.2908392984796393 1103 54-55 0.40722735809564625 1103 56-57 0.17747275110016858 1103 58-59 0.15971020314874096 1103 60-61 0.20057285189176355 1103 62-63 0.14924192643729128 1103 64-65 0.17612171019330702 1103 66-67 0.2905900967086481 1103 68-69 0.26304363456501534 1103 70-71 0.12951300591182502 1103 72-73 0.033615853101384374 1103 74-75 0.12972780380414406 1103 76-77 0.13750006149297533 1103 78-79 0.28405075323765416 1103 80-81 0.2738224940964926 1103 82-83 0.4517790464151439 1103 84-85 0.4238110307715637 1103 86-87 0.4517735813110768 1103 88-89 0.4378704169945884 1103 90-91 0.5369627529877157 1103 92-93 0.5998965390501318 1103 94-95 0.45520911770245576 1103 96-97 0.610759623645837 1103 98-99 0.8487068552041954 1103 100-101 0.9733183593039456 1104 1 0.05749094765591778 1104 2 0.1386755615300821 1104 3 0.045024645898863014 1104 4 -0.08119929867937259 1104 5 -0.11074549798281197 1104 6 -0.03174799589913846 1104 7 0.008460063052801559 1104 8 -0.028791927373255533 1104 9 0.17773496692260693 1104 10-11 -0.024989671659213286 1104 12-13 0.21917162747563168 1104 14-15 0.10666077556524556 1104 16-17 0.16947197343326081 1104 18-19 0.0083388590846738 1104 20-21 0.3683520385729935 1104 22-23 0.2826839332745692 1104 24-25 0.026287502365399007 1104 26-27 0.07802855681428866 1104 28-29 0.11964111980947933 1104 30-31 0.03710474016113352 1104 32-33 0.08875076500745394 1104 34-35 -0.0033158504195114347 1104 36-37 0.25768318453233263 1104 38-39 0.37257413460941535 1104 40-41 0.38062005130385757 1104 42-43 0.22916404570354132 1104 44-45 0.3672078772515661 1104 46-47 0.32243126657809995 1104 48-49 0.3548783235353099 1104 50-51 0.4168894632341349 1104 52-53 0.3492140535338848 1104 54-55 0.33585016553243463 1104 56-57 0.3382252722491472 1104 58-59 0.36350387518429983 1104 60-61 0.25223967787274404 1104 62-63 0.3797908438965436 1104 64-65 0.43535915224272514 1104 66-67 0.5907537921634187 1104 68-69 0.360345646704765 1104 70-71 0.29802134601601793 1104 72-73 0.43214737343785714 1104 74-75 0.44659082535937245 1104 76-77 0.5049987471499051 1104 78-79 0.30216598527895044 1104 80-81 0.5120423956402469 1104 82-83 0.5358372598644614 1104 84-85 0.46289839873982785 1104 86-87 0.4802960207318101 1104 88-89 0.5033772754756853 1104 90-91 0.4530455327038183 1104 92-93 0.45489821185039503 1104 94-95 0.9222907981498096 1104 96-97 0.9348790137906526 1104 98-99 0.4914265894679275 1104 100-101 0.4485322387667381 1105 1 -0.09758151611219645 1105 2 0.11395472522692529 1105 3 0.06775371852101841 1105 4 -0.010675817250685782 1105 5 0.02974313748087809 1105 6 0.023827358500170703 1105 7 -0.12235485735584462 1105 8 9.854542313547654E-5 1105 9 -0.16332783500975978 1105 10-11 0.0018060834686863814 1105 12-13 -0.043794635294617024 1105 14-15 -0.06773535970045685 1105 16-17 -0.032597005738359996 1105 18-19 -0.026501166257851594 1105 20-21 0.15809227774303025 1105 22-23 0.1440841075043977 1105 24-25 0.12415120712503835 1105 26-27 0.18600155119140993 1105 28-29 0.11552889996311677 1105 30-31 0.025421442465720645 1105 32-33 -0.014939739467088486 1105 34-35 -0.10573328924108694 1105 36-37 0.14366164339033816 1105 38-39 0.2317132808832767 1105 40-41 0.13968198208710447 1105 42-43 0.027760307681838015 1105 44-45 0.22904362604383977 1105 46-47 0.06244077002310178 1105 48-49 -0.004555825510387024 1105 50-51 -0.0750761659731154 1105 52-53 0.15321341997088211 1105 54-55 0.3263506803023759 1105 56-57 0.3639162518959296 1105 58-59 0.023460713704928082 1105 60-61 0.28108263344413587 1105 62-63 0.08246962745558761 1105 64-65 -0.034974260285984826 1105 66-67 0.2925911248631863 1105 68-69 0.14462734465360683 1105 70-71 0.2221046595505065 1105 72-73 0.18222854869722127 1105 74-75 0.00930168304528678 1105 76-77 0.055450160787348324 1105 78-79 0.23041101576916745 1105 80-81 0.06510923653674183 1105 82-83 -0.01627527676339824 1105 84-85 0.12672789109747384 1105 86-87 0.14478283470187492 1105 88-89 0.06953511185804118 1105 90-91 0.29989355677921026 1105 92-93 0.2398748492147753 1105 94-95 0.504325327333305 1105 96-97 0.051939677447894184 1105 98-99 0.1896189550337759 1105 100-101 0.29809864408866815 1106 1 0.0051419543673283385 1106 2 -0.02595384397840661 1106 3 -0.0723039931153302 1106 4 0.22278720515309658 1106 5 0.1919225623098484 1106 6 0.07551169530268709 1106 7 0.18795542374521546 1106 8 0.1729611074307229 1106 9 0.1343345194953116 1106 10-11 0.08175291094980253 1106 12-13 -0.09218000636747092 1106 14-15 0.008226771090974694 1106 16-17 0.06452551801096007 1106 18-19 0.07180477025935517 1106 20-21 0.030071332958144126 1106 22-23 0.25283649864663715 1106 24-25 0.03285496486585515 1106 26-27 0.1297164946145557 1106 28-29 0.20305605361225076 1106 30-31 0.14716543624064116 1106 32-33 0.13784857554767171 1106 34-35 0.1819089634580635 1106 36-37 0.18189560247343195 1106 38-39 0.058725893145378905 1106 40-41 0.22432031169187638 1106 42-43 0.052438516675778146 1106 44-45 -0.08939167532113856 1106 46-47 0.33417990045788315 1106 48-49 0.4738233853316203 1106 50-51 0.44953881959897757 1106 52-53 0.6011302892767247 1106 54-55 0.4527845550875682 1106 56-57 0.4632775332239447 1106 58-59 0.3487873680511342 1106 60-61 0.4113569258281444 1106 62-63 0.5644655422498701 1106 64-65 0.7504616571020719 1106 66-67 0.7667797576161561 1106 68-69 0.5659595756298401 1106 70-71 0.07752404158208392 1106 72-73 0.5968785503183369 1106 74-75 0.6211828230122123 1106 76-77 0.5343675812717379 1106 78-79 0.5042105813107831 1106 80-81 0.1608862361800938 1106 82-83 0.1426376046035216 1106 84-85 0.346991808355849 1106 86-87 0.22769305744285617 1106 88-89 0.5061544420139903 1106 90-91 0.5354234987433557 1106 92-93 0.5755733869705075 1106 94-95 0.6352496861829628 1106 96-97 0.5307338933153538 1106 98-99 0.38870626820046184 1106 100-101 0.8042653593887366 1107 1 0.07911579831302618 1107 2 -0.010734901285985643 1107 3 0.13135560933188728 1107 4 0.08783335110420154 1107 5 0.042687919570120414 1107 6 -0.07153392143334258 1107 7 0.12608937930718866 1107 8 0.15443857970706887 1107 9 0.0995987662057587 1107 10-11 0.19294420616360952 1107 12-13 0.24469946890776129 1107 14-15 0.19925336815784078 1107 16-17 0.2952612873373326 1107 18-19 0.2539569917599138 1107 20-21 0.21179857871440078 1107 22-23 0.16060272991362012 1107 24-25 0.20426652580977844 1107 26-27 0.11605713271964646 1107 28-29 0.04211453187834735 1107 30-31 0.20473160817139302 1107 32-33 0.029052780402011535 1107 34-35 0.14760419972052574 1107 36-37 0.08745677091617665 1107 38-39 0.05650679810316461 1107 40-41 -0.17692494742305342 1107 42-43 -0.2625466893572721 1107 44-45 -0.3180967822466343 1107 46-47 -0.11355008759468177 1107 48-49 -0.2068414210638636 1107 50-51 -0.28465488915632875 1107 52-53 -0.334854732332893 1107 54-55 -0.07125463890182715 1107 56-57 -0.004885368799094181 1107 58-59 -0.3416839541045391 1107 60-61 0.024218054980686077 1107 62-63 0.180900877780239 1107 64-65 0.17776274038930495 1107 66-67 -0.13222836503344837 1107 68-69 0.010442107523068955 1107 70-71 -0.020589226481387612 1107 72-73 -0.07486978385692922 1107 74-75 0.018184338491757046 1107 76-77 -0.0932434135724236 1107 78-79 -0.022194043708264388 1107 80-81 0.1153196152183682 1107 82-83 0.08479479751845531 1107 84-85 0.003731683108281203 1107 86-87 -0.016226982969932635 1107 88-89 0.03809778392702512 1107 90-91 0.21137347990008948 1107 92-93 0.37757678677645856 1107 94-95 0.6867158525008286 1107 96-97 0.6098535520518134 1107 98-99 0.6175136098569034 1107 100-101 0.7294736375954969 1108 1 0.10649493171966284 1108 2 -0.03897558252756639 1108 3 -0.06561040950792574 1108 4 0.04036254139203521 1108 5 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-0.446088616453423 2316 92-93 -0.37356326740766477 2316 94-95 -0.00534331252516651 2316 96-97 -0.2681732707728308 2316 98-99 -0.396772825601726 2316 100-101 -0.534261701560105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3633.0 3 797.0 4 714.0 5 800.0 6 910.0 7 1123.0 8 1282.0 9 1691.0 10 2077.0 11 2680.0 12 3411.0 13 4376.0 14 4988.0 15 6086.0 16 7476.0 17 9509.0 18 8372.0 19 6156.0 20 6243.0 21 6581.0 22 7155.0 23 7800.0 24 8930.0 25 10175.0 26 11691.0 27 13506.0 28 15949.0 29 19219.0 30 22945.0 31 28498.0 32 35849.0 33 45881.0 34 61771.0 35 85329.0 36 120805.0 37 160112.0 38 116197.0 39 11913.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.929425106865565 19.0722614789613 15.154264445026024 22.84404896914711 2 14.750595011783965 19.761948778409124 41.733725395812186 23.75373081399472 3 18.880078134083668 24.35109178429412 30.15208241285492 26.616747668767292 4 12.286888629215573 16.516036765090604 38.14256065019098 33.05451395550285 5 13.479499523244726 37.063303797762735 34.76115723621479 14.696039442777739 6 31.492281941171075 36.21824990736471 17.95161591956353 14.33785223190069 7 28.1764612273392 32.765479966964925 20.90955659348382 18.148502212212062 8 25.727292051680863 35.18566057110068 20.115948560748706 18.971098816469762 9 25.864187779641405 17.09361105045277 20.919500443709317 36.122700726196506 10-11 24.523367134940607 26.7461533949038 27.771411379248455 20.959068090907138 12-13 25.44875160289171 24.130837778066866 28.277797394487912 22.142613224553514 14-15 22.32979260919814 25.417482156028036 26.996230842049634 25.25649439272419 16-17 21.64953962297465 28.79869852666574 27.391169752012644 22.160592098346964 18-19 21.37482075850297 27.135986581788423 30.208050810790095 21.281141848918512 20-21 22.219467026403617 26.284800397677348 30.270168565719256 21.225564010199776 22-23 21.813872945155364 26.265310436299526 30.079243775362535 21.841572843182572 24-25 21.623533347116172 26.392144245935416 30.331926399635762 21.65239600731265 26-27 21.540665566409718 26.723235823932562 30.060243132782276 21.67585547687544 28-29 21.26358206389291 26.789161762758486 30.380030619902005 21.567225553446598 30-31 21.513253924598683 26.47003643616759 30.26699526788686 21.749714371346872 32-33 21.32547399139058 26.603113493645164 30.092071168556284 21.979341346407967 34-35 21.77009911974234 26.677295973932058 29.86260313477439 21.690001771551213 36-37 21.697725909464914 26.52944971340873 29.885915132286065 21.886909244840282 38-39 21.606079801752664 26.66265263808191 29.96435565841584 21.766911901749587 40-41 21.806939144289885 26.59693689132096 29.893123334951444 21.70300062943771 42-43 21.678751746558277 26.60692072628949 29.650142454113283 22.064185073038946 44-45 21.719932142635205 26.96790132921218 29.382924266790255 21.929242261362365 46-47 21.727603191740826 27.019390539888338 28.990968272507157 22.262037995863675 48-49 21.839326794800538 27.025316051348796 28.927228766756514 22.20812838709415 50-51 21.839730692922092 27.135963832189535 28.7432801007346 22.281025374153767 52-53 21.94079761480106 27.18334357763574 28.630578836007114 22.245279971556084 54-55 21.705611832160148 27.14240997566133 28.766091485762367 22.38588670641615 56-57 21.843316313437047 27.520789085280246 28.522415981438755 22.113478619843953 58-59 21.900600923157317 27.18251226521903 28.66432490492641 22.252561906697245 60-61 22.051954023522363 27.020295556435443 28.98140202943953 21.946348390602665 62-63 21.825645432402695 27.093790552721803 28.896036973921426 22.184527040954073 64-65 21.78893036920058 27.018526797378033 28.84589257824974 22.346650255171653 66-67 21.722371531037556 27.36839293977349 28.62583830174625 22.28339722744271 68-69 21.753527836858122 27.267770699749338 28.6942924613481 22.28440900204444 70-71 21.76452589267622 27.076717986949006 28.679671211115696 22.479084909259075 72-73 21.810662554489063 27.134101734592686 28.60475486018054 22.450480850737705 74-75 21.717554105038733 27.223627966821073 28.391486258475595 22.667331669664602 76-77 21.750137909009087 27.13846064512383 28.47951688296606 22.631884562901025 78-79 21.814898367433447 27.167324280616796 28.47604862568846 22.541728726261297 80-81 21.590001886984165 27.415383275514206 28.427445458900035 22.5671693786016 82-83 21.77928785753435 27.301213689187808 28.38571906323446 22.53377939004338 84-85 21.84946446309588 27.026195415091177 28.27921650042352 22.84512362138943 86-87 21.837939386339517 27.080677334782376 28.400607019034947 22.68077625984316 88-89 21.788914789666656 26.953265384916847 28.45478798294226 22.803031842474244 90-91 21.708444792313237 26.930502434920246 28.589104958504453 22.771947814262067 92-93 21.853625627678284 26.805324372292688 28.611563156235505 22.729486843793527 94-95 21.63074963320442 26.933623789371374 28.44760266357167 22.988023913852533 96-97 21.614963450674377 26.96231268107738 28.424808253935076 22.997915614313168 98-99 21.6616480146228 26.82927254125727 28.282320370772517 23.226759073347417 100-101 21.96330499968944 26.288873138300804 28.766107830383582 22.981714031626176 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1059.0 1 607.5 2 162.0 3 148.0 4 122.5 5 126.0 6 207.0 7 431.0 8 748.0 9 903.5 10 821.0 11 741.5 12 825.0 13 938.0 14 990.5 15 1083.0 16 1178.0 17 1272.0 18 1347.0 19 1432.0 20 1643.0 21 1912.5 22 2264.0 23 2785.0 24 3549.5 25 4367.0 26 5435.5 27 6831.5 28 8065.0 29 9344.0 30 10894.0 31 12447.5 32 13861.0 33 15649.5 34 17915.0 35 19549.0 36 21246.0 37 23225.0 38 24772.5 39 26075.5 40 27240.0 41 28694.5 42 29981.0 43 31049.5 44 32168.0 45 32829.0 46 32774.0 47 33274.0 48 34923.0 49 35114.5 50 33577.5 51 31856.5 52 29762.0 53 28055.0 54 26614.5 55 24328.5 56 21638.5 57 19063.5 58 16712.5 59 14530.0 60 12199.0 61 9722.5 62 7504.5 63 5756.0 64 4306.0 65 3232.5 66 2394.0 67 1816.5 68 1365.0 69 929.0 70 675.0 71 513.0 72 415.5 73 320.0 74 241.0 75 194.5 76 137.0 77 84.0 78 53.5 79 40.5 80 27.0 81 21.0 82 12.5 83 7.0 84 8.0 85 7.0 86 4.0 87 3.0 88 2.0 89 1.0 90 2.0 91 2.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3367608360479 2 0.2971146377937238 3 0.2979261096878152 4 0.298505732469309 5 0.307200074191716 6 0.20020170872795984 7 0.20020170872795984 8 0.2006654069531549 9 0.19776729304568585 10-11 0.20559220059585223 12-13 0.19544880191971065 14-15 0.1964921229263995 16-17 0.20396925680766956 18-19 0.1982889535490303 20-21 0.18901498904512942 22-23 0.18733408297879742 24-25 0.19197106523074783 26-27 0.18768185664769368 28-29 0.20309982263542886 30-31 0.20976548462260763 32-33 0.21196805119228407 34-35 0.2089540127285163 36-37 0.18090027010421617 38-39 0.1719740792692116 40-41 0.1789875149252866 42-43 0.19139144244925405 44-45 0.19782525532383524 46-47 0.19944819911201792 48-49 0.2009552183439018 50-51 0.20333167174802638 52-53 0.23057394247823515 54-55 0.19179717839629967 56-57 0.1716842678784647 58-59 0.16867022941469692 60-61 0.1631058507123564 62-63 0.17185815471291283 64-65 0.16751098385170932 66-67 0.1668733987920661 68-69 0.1755677405144732 70-71 0.17035113548102895 72-73 0.16785875752060558 74-75 0.1716842678784647 76-77 0.180262685044573 78-79 0.1811321192168137 80-81 0.17011928636843143 82-83 0.16855430485839815 84-85 0.1613090200897256 86-87 0.15904849124189976 88-89 0.1666415496794686 90-91 0.1754518159581744 92-93 0.16467083222238965 94-95 0.1687281916928463 96-97 0.16982947497768452 98-99 0.17493015545483 100-101 0.14913694167835573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 862630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.3166107992597 #Duplication Level Percentage of deduplicated Percentage of total 1 83.17257352514838 57.65240908215522 2 10.754663728866038 14.909536799414447 3 2.8681477773177475 5.964308495852883 4 1.155885682261803 3.2048831186311264 5 0.5737881342127003 1.988652439022757 6 0.3274639407142771 1.361921431757004 7 0.22075368063302617 1.0711327874060486 8 0.1535234931273953 0.8513382577323586 9 0.11965691971047449 0.7464790919708303 >10 0.6008230155309017 7.609175555965581 >50 0.03648763751759415 1.7343000949224716 >100 0.015395740009678333 1.7388082207422786 >500 1.67344989989019E-4 0.08467844904400634 >1k 6.69379959956076E-4 1.0823761753830454 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3585 0.41558953433105733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2747 0.3184447561526958 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1826 0.21167823980153716 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1173 0.13597950453844637 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1592455629876077E-4 0.0 10-11 0.0 0.0 0.0 1.1592455629876077E-4 0.0 12-13 0.0 0.0 0.0 1.1592455629876077E-4 0.0 14-15 0.0 0.0 0.0 1.1592455629876077E-4 0.0 16-17 0.0 0.0 0.0 3.477736688962823E-4 0.0 18-19 0.0 0.0 0.0 3.477736688962823E-4 0.0 20-21 0.0 0.0 0.0 3.477736688962823E-4 0.0 22-23 0.0 0.0 0.0 3.477736688962823E-4 0.0 24-25 0.0 0.0 0.0 3.477736688962823E-4 0.0 26-27 0.0 0.0 0.0 4.0573594704566265E-4 0.0 28-29 0.0 0.0 0.0 6.375850596431842E-4 0.0 30-31 0.0 0.0 0.0 0.00150701923188389 0.0 32-33 0.0 0.0 0.0 0.002724227073020878 0.0 34-35 0.0 0.0 0.0 0.0036516235234109644 0.0 36-37 0.0 0.0 0.0 0.005854190093087418 0.0 38-39 0.0 0.0 0.0 0.00782490755016635 0.0 40-41 0.0 0.0 0.0 0.01054913462318723 0.0 42-43 0.0 0.0 0.0 0.015707777378482085 0.0 44-45 0.0 0.0 0.0 0.024807855047934806 0.0 46-47 0.0 0.0 0.0 0.03616846156521336 0.0 48-49 0.0 0.0 0.0 0.05796227814938038 0.0 50-51 0.0 0.0 0.0 0.07749556588572157 0.0 52-53 0.0 0.0 0.0 0.10027474119842805 5.7962278149380385E-5 54-55 0.0 0.0 0.0 0.13980501489630548 1.1592455629876077E-4 56-57 0.0 0.0 0.0 0.17863974125639032 1.1592455629876077E-4 58-59 0.0 0.0 0.0 0.22640065845147977 1.1592455629876077E-4 60-61 0.0 0.0 0.0 0.2764221044943951 1.1592455629876077E-4 62-63 0.0 0.0 0.0 0.34238317702838994 1.1592455629876077E-4 64-65 0.0 0.0 0.0 0.3940855291376372 1.1592455629876077E-4 66-67 0.0 0.0 0.0 0.4707696231292675 1.1592455629876077E-4 68-69 0.0 0.0 0.0 0.5723774967251313 1.1592455629876077E-4 70-71 0.0 0.0 0.0 0.6491775152730603 1.1592455629876077E-4 72-73 0.0 0.0 0.0 0.7695651669893233 1.1592455629876077E-4 74-75 0.0 0.0 0.0 0.9178906367735877 1.1592455629876077E-4 76-77 0.0 0.0 0.0 1.040596779615826 1.1592455629876077E-4 78-79 0.0 0.0 0.0 1.1639405075177076 1.1592455629876077E-4 80-81 0.0 0.0 0.0 1.337595492853251 1.7388683444814117E-4 82-83 0.0 0.0 0.0 1.544346939012091 2.3184911259752154E-4 84-85 0.0 0.0 0.0 1.753880574522101 2.3184911259752154E-4 86-87 0.0 0.0 0.0 2.0463582300638743 2.3184911259752154E-4 88-89 0.0 0.0 0.0 2.391639520999733 2.3184911259752154E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2090 0.0 43.458107 1 GTATCAA 3395 0.0 35.717754 1 CTAGCGC 55 0.0025789577 34.5463 8 ATCAACG 4235 0.0 28.40206 3 CAACGCA 4310 0.0 28.023022 5 TCAACGC 4295 0.0 28.008547 4 TATCAAC 4325 0.0 27.92096 2 AACGCAG 4450 0.0 27.00471 6 GTACATG 3265 0.0 25.051228 1 TACATGG 3185 0.0 24.928135 2 GTGTAGC 645 0.0 23.592503 1 ACGCAGA 5155 0.0 23.312887 7 CGCAGAG 5155 0.0 23.03645 8 GTAGCCC 560 0.0 22.922316 3 ACATGGG 3345 0.0 22.882977 3 GTATATG 400 0.0 22.587976 1 CATGGGG 1525 0.0 22.137152 4 GAGTACT 3270 0.0 21.281101 12-13 GCAGAGT 5525 0.0 20.891914 9 GTACTTT 3385 0.0 20.417782 14-15 >>END_MODULE