FastQCFastQC Report
Thu 26 May 2016
SRR937755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862630
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39320.4558153553667273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31870.36945156092415055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22260.2580480623210415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.043.8666571
GTATCAA41450.035.885291
ATCAACG50850.028.6842383
TATCAAC51950.027.9854162
TCAACGC51650.027.9639914
CAACGCA52150.027.695885
AACGCAG53650.026.913736
ACGCAGA61200.023.130517
CGCAGAG61000.023.05068
GTACATG34000.022.922481
TACATGG34600.021.9704912
GAGTACT37250.021.93423812-13
GCAGAGT64800.021.7721779
GTATAGC3500.021.724441
GTACACG1100.002868995821.6010061
GTACTTT38600.021.28770414-15
ACATGGG34750.020.6451513
AGTACTT38850.020.41953512-13
GGGCGTA1404.8483704E-420.3583989
GTACTAC2401.4129728E-719.7905926
Produced by FastQC (version 0.11.5)