##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937755_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 862630 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16373995803531 33.0 31.0 34.0 31.0 34.0 2 32.44773657303827 34.0 31.0 34.0 31.0 34.0 3 32.54253156046045 34.0 31.0 34.0 31.0 34.0 4 36.16512525648308 37.0 35.0 37.0 35.0 37.0 5 36.07207261514206 37.0 35.0 37.0 35.0 37.0 6 36.11009586960806 37.0 35.0 37.0 35.0 37.0 7 36.057941411729246 37.0 35.0 37.0 35.0 37.0 8 36.03364942095684 37.0 35.0 37.0 35.0 37.0 9 37.735612023694976 39.0 37.0 39.0 35.0 39.0 10-11 37.655730150817845 39.0 37.0 39.0 35.0 39.0 12-13 37.543154654950555 39.0 37.0 39.0 35.0 39.0 14-15 38.77623256784484 40.0 38.0 41.0 35.0 41.0 16-17 38.730165308417284 40.0 38.0 41.0 35.0 41.0 18-19 38.703413978182994 40.0 38.0 41.0 34.5 41.0 20-21 38.60371885976606 40.0 38.0 41.0 34.0 41.0 22-23 38.54230376870733 40.0 38.0 41.0 34.0 41.0 24-25 38.569461994134215 40.0 38.0 41.0 34.0 41.0 26-27 38.43266580109665 40.0 38.0 41.0 34.0 41.0 28-29 38.2651496006399 40.0 38.0 41.0 34.0 41.0 30-31 38.33355088508399 40.0 38.0 41.0 34.0 41.0 32-33 38.27145010027474 40.0 38.0 41.0 34.0 41.0 34-35 38.13643856578139 40.0 38.0 41.0 34.0 41.0 36-37 37.99575368350278 40.0 37.0 41.0 33.0 41.0 38-39 37.87165702560773 40.0 37.0 41.0 33.0 41.0 40-41 37.690127864785595 40.0 37.0 41.0 33.0 41.0 42-43 37.47010073843943 40.0 37.0 41.0 33.0 41.0 44-45 37.16897221288386 40.0 37.0 41.0 32.0 41.0 46-47 36.804863035136734 40.0 36.0 41.0 31.0 41.0 48-49 36.486860531166315 39.5 36.0 41.0 30.0 41.0 50-51 36.22572829602495 39.0 36.0 41.0 30.0 41.0 52-53 36.00044109293672 39.0 35.0 40.5 29.0 41.0 54-55 35.770889605045035 39.0 35.0 40.0 28.5 41.0 56-57 35.50769912940658 38.5 35.0 40.0 28.0 41.0 58-59 35.248302284873006 38.0 35.0 40.0 28.0 41.0 60-61 34.924365602865656 38.0 34.0 40.0 27.0 41.0 62-63 34.54882742311304 37.5 34.0 40.0 26.0 41.0 64-65 34.13627685102536 37.0 34.0 40.0 26.0 41.0 66-67 33.71139654312973 36.5 33.0 39.0 25.5 40.5 68-69 33.277214448836695 36.0 32.5 39.0 24.0 40.0 70-71 32.87347646151885 35.5 32.0 38.5 23.5 40.0 72-73 32.38214182210217 35.0 32.0 38.0 22.0 40.0 74-75 31.89440432166746 35.0 31.0 37.0 20.5 39.0 76-77 30.664058750565133 34.0 29.5 36.0 20.0 38.5 78-79 31.2580868970474 34.0 31.0 36.0 20.0 39.0 80-81 31.063218297531968 34.0 31.0 36.0 20.0 37.5 82-83 30.691682992708344 34.0 31.0 35.0 18.0 37.0 84-85 30.305245586172518 34.0 30.5 35.0 17.5 36.5 86-87 29.912267716170316 34.0 30.0 35.0 10.0 36.0 88-89 29.471816421872646 34.0 29.5 35.0 7.0 36.0 90-91 28.917898171869744 34.0 29.0 35.0 2.0 35.0 92-93 28.471032192249282 34.0 29.0 35.0 2.0 35.0 94-95 27.991061637086585 33.0 28.0 35.0 2.0 35.0 96-97 27.415778491357827 33.0 27.0 35.0 2.0 35.0 98-99 26.500148383432062 32.5 25.5 34.0 2.0 35.0 100-101 23.74779569456198 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1976722606728103 1101 2 0.086604451020861 1101 3 0.1921662079799873 1101 4 0.11743845331179159 1101 5 -0.025691084851217738 1101 6 0.04722054090638039 1101 7 0.02522347446716111 1101 8 0.0860803532617993 1101 9 0.13190156648506957 1101 10-11 0.12221865071982307 1101 12-13 0.07664402261886494 1101 14-15 0.18814656154408027 1101 16-17 0.11225878692685143 1101 18-19 0.15123323997680416 1101 20-21 0.12217873431266213 1101 22-23 0.041350149706538275 1101 24-25 0.06993037806117997 1101 26-27 0.10563386978515865 1101 28-29 -0.047391002784110015 1101 30-31 0.03888399288764788 1101 32-33 0.17999014304326266 1101 34-35 -0.00965884055143107 1101 36-37 0.026056461135553377 1101 38-39 0.10612216628695137 1101 40-41 0.14066908802004718 1101 42-43 0.013360025389658858 1101 44-45 0.09036269760333226 1101 46-47 0.09893082050708557 1101 48-49 0.33182885328251643 1101 50-51 0.24290664391926953 1101 52-53 0.19633961926233923 1101 54-55 0.07124614706733468 1101 56-57 0.07047430699117996 1101 58-59 -0.027013014479926767 1101 60-61 -0.006562886536826795 1101 62-63 -0.1303612601722861 1101 64-65 0.019020336926587333 1101 66-67 -0.1419810510562769 1101 68-69 -0.012135742683028639 1101 70-71 0.12683229185811484 1101 72-73 -0.15156527799801722 1101 74-75 -0.03523347462703441 1101 76-77 -0.20663662617480227 1101 78-79 -0.1823432453138807 1101 80-81 0.002029844955128368 1101 82-83 0.013037999256795274 1101 84-85 -0.013624565187175364 1101 86-87 -0.17642836789701377 1101 88-89 -0.3608022635389325 1101 90-91 -0.2357593026013305 1101 92-93 -0.36017579180943926 1101 94-95 -0.49496941546730255 1101 96-97 -0.7751628716458718 1101 98-99 -0.7124847696957133 1101 100-101 -0.6612205171730992 1102 1 0.20083513116485818 1102 2 0.19728301723490915 1102 3 0.09580542538398618 1102 4 0.10137862705978051 1102 5 -0.03754318004759938 1102 6 0.020483973524278554 1102 7 0.08163043168719497 1102 8 0.051169234850348744 1102 9 0.017324620945572633 1102 10-11 0.059323520043811584 1102 12-13 0.12783580244073534 1102 14-15 0.1834090998708504 1102 16-17 0.04747469435859131 1102 18-19 0.06259028115069754 1102 20-21 0.09274879928564417 1102 22-23 0.09386283404001716 1102 24-25 -0.013457251519788826 1102 26-27 0.0679212475045361 1102 28-29 -0.049985359624521664 1102 30-31 0.20809391404401367 1102 32-33 0.23508121473255983 1102 34-35 0.22510581487271963 1102 36-37 -0.04874141081655381 1102 38-39 0.23036430017510412 1102 40-41 0.19254573059561153 1102 42-43 0.19251482901062644 1102 44-45 0.2048324194605442 1102 46-47 0.18425897060198793 1102 48-49 0.23374245380814074 1102 50-51 0.22189855879735632 1102 52-53 0.35093700574211795 1102 54-55 0.24525010749950837 1102 56-57 0.28114694168238685 1102 58-59 0.1382127484051594 1102 60-61 0.20083326618620134 1102 62-63 0.053642335243104355 1102 64-65 0.08585673640095592 1102 66-67 0.16848808677406168 1102 68-69 0.2223884579302009 1102 70-71 -0.11318997416612575 1102 72-73 -0.08828368579477086 1102 74-75 -0.16513428131414187 1102 76-77 -0.2022450179108226 1102 78-79 -0.02671286131621642 1102 80-81 -0.018632843028516533 1102 82-83 -0.05999501651193029 1102 84-85 -0.014264257113005385 1102 86-87 -0.2752981846585847 1102 88-89 -0.34598486318851585 1102 90-91 -0.4342937601863781 1102 92-93 -0.32042728837827994 1102 94-95 -0.44210996518259194 1102 96-97 -0.49514434704373755 1102 98-99 -0.4642801960353218 1102 100-101 -0.3972517808612288 1103 1 0.16358618396692748 1103 2 0.07836092458832411 1103 3 0.027106040868275727 1103 4 0.06132558203224647 1103 5 -0.07000997628990291 1103 6 -0.057105514651944134 1103 7 0.07154985183620965 1103 8 -0.02035854292742556 1103 9 0.12890949298962795 1103 10-11 0.046262700477889496 1103 12-13 0.09707373570928013 1103 14-15 -0.08468188652371111 1103 16-17 -0.07801323890995349 1103 18-19 0.06592179232659845 1103 20-21 -0.05391159106096666 1103 22-23 -0.18141757574294104 1103 24-25 -0.05587606428299097 1103 26-27 -0.07371909911029917 1103 28-29 -0.015386639183965656 1103 30-31 0.04284245631806982 1103 32-33 -0.06839505672840573 1103 34-35 0.0348654925533225 1103 36-37 -0.10881287988036803 1103 38-39 0.011870414525603223 1103 40-41 -0.16313028981255684 1103 42-43 -0.0894235415757052 1103 44-45 -0.009638547497665684 1103 46-47 -0.06525107151009735 1103 48-49 -0.05836760450090139 1103 50-51 -0.07731082733806005 1103 52-53 -0.023581134847454166 1103 54-55 -0.2075185594910991 1103 56-57 -0.05785633820099889 1103 58-59 -0.09474603034443163 1103 60-61 0.02258780133359295 1103 62-63 -0.006580980273575676 1103 64-65 -0.22562285543577332 1103 66-67 -0.28369748983752885 1103 68-69 -0.13512490455764947 1103 70-71 -0.21260283454837037 1103 72-73 -0.4741953718977854 1103 74-75 -0.4745740691340288 1103 76-77 -0.35588832046994057 1103 78-79 -0.3545756731076004 1103 80-81 -0.25990875815647385 1103 82-83 -0.07735106607461262 1103 84-85 -0.1318885687424185 1103 86-87 -0.4338937062005357 1103 88-89 -0.4319147708658768 1103 90-91 -0.3088361962084072 1103 92-93 -0.41557225112528684 1103 94-95 -0.4897016804401204 1103 96-97 -0.484568747756402 1103 98-99 -0.3951343829481857 1103 100-101 -0.5488400947582193 1104 1 0.13633148817760343 1104 2 0.10659540339155882 1104 3 0.059785388954118446 1104 4 0.00888090392681562 1104 5 -0.11463789771608646 1104 6 -0.006030143051312109 1104 7 -0.0032625555623511104 1104 8 -0.05396510030447388 1104 9 -0.03734295646790997 1104 10-11 -0.03098841073943248 1104 12-13 0.0055452742075843275 1104 14-15 -0.05304923346248103 1104 16-17 0.026629976690102808 1104 18-19 -0.007713680597937866 1104 20-21 -0.13256426993110892 1104 22-23 -5.759182368478832E-4 1104 24-25 -0.1816551112647744 1104 26-27 -0.030496329908942243 1104 28-29 -0.08446851080849171 1104 30-31 -0.06926264333303322 1104 32-33 -0.03371432260806273 1104 34-35 -0.06144359314185266 1104 36-37 0.05053568390110996 1104 38-39 -0.10842782186496436 1104 40-41 -0.10853008907652395 1104 42-43 0.15548158675196788 1104 44-45 0.025204687220103494 1104 46-47 0.02198665730508509 1104 48-49 0.2291317980704335 1104 50-51 0.11961599959881397 1104 52-53 0.12441150483137164 1104 54-55 0.08156044447583355 1104 56-57 0.17607522037091172 1104 58-59 0.0888844241792981 1104 60-61 0.08251587092208723 1104 62-63 0.06005740810285687 1104 64-65 0.1721272282042392 1104 66-67 0.139634489324159 1104 68-69 0.1860838131214564 1104 70-71 -0.06835846232641529 1104 72-73 -0.2958485765051577 1104 74-75 -0.14427248714292062 1104 76-77 -0.13396746781974755 1104 78-79 0.07699843194425426 1104 80-81 -0.0666405843764224 1104 82-83 -0.18801755749553806 1104 84-85 -0.12518183452648302 1104 86-87 -0.22243502436714735 1104 88-89 -0.2841849998005408 1104 90-91 -0.1056280042665243 1104 92-93 0.005096391038840409 1104 94-95 -0.14793305261428102 1104 96-97 0.04837227408558675 1104 98-99 0.18090992236176007 1104 100-101 -0.23948197211806033 1105 1 0.12577474469142658 1105 2 0.027415867476456413 1105 3 0.11669914519075064 1105 4 0.03136051111775373 1105 5 -0.0437026003374541 1105 6 0.003002089466313862 1105 7 -0.0385084572016936 1105 8 -0.0213247265023071 1105 9 0.07269901708083637 1105 10-11 0.08580298864284686 1105 12-13 0.09238894253735452 1105 14-15 0.06096042837326365 1105 16-17 0.05348314846487057 1105 18-19 0.17048581255166795 1105 20-21 -0.08631219105251375 1105 22-23 -0.16841071668461893 1105 24-25 -0.202693362630896 1105 26-27 -0.3132079795486007 1105 28-29 -0.1290614465810478 1105 30-31 -0.10002806663231212 1105 32-33 -0.13173641811768988 1105 34-35 -0.08067618995819714 1105 36-37 -0.18130481661365394 1105 38-39 -0.11101553074276893 1105 40-41 -0.05429907616768048 1105 42-43 -0.12617478451594621 1105 44-45 -0.12945200082931052 1105 46-47 -0.09321687480863972 1105 48-49 -0.27443991334162376 1105 50-51 0.1362252496186116 1105 52-53 0.3743065709595115 1105 54-55 0.25637631522898374 1105 56-57 0.3692960962717535 1105 58-59 0.3342574343955036 1105 60-61 0.2511050053166386 1105 62-63 0.007461779687552905 1105 64-65 -0.015049060700491168 1105 66-67 0.08178820754701377 1105 68-69 0.38836068015242375 1105 70-71 0.17346461520585876 1105 72-73 0.2601009760409738 1105 74-75 0.22065980081146108 1105 76-77 0.06648988547081913 1105 78-79 0.23373399858716226 1105 80-81 0.14697706035312663 1105 82-83 0.051584089768265784 1105 84-85 0.02060893129279151 1105 86-87 -0.09201014118032802 1105 88-89 -0.11079464579721687 1105 90-91 0.2028439209730415 1105 92-93 0.1843432430226848 1105 94-95 0.25757300583190457 1105 96-97 0.3118453872557758 1105 98-99 0.16472040782941022 1105 100-101 0.17821621430785228 1106 1 0.06644847941242915 1106 2 0.07193812536317523 1106 3 0.059097744548793685 1106 4 0.053145032727194064 1106 5 0.012475462756277977 1106 6 -0.06382344631165893 1106 7 -0.05304984422630099 1106 8 -0.07852409102586222 1106 9 -0.07299044243995922 1106 10-11 -0.16883177451249054 1106 12-13 -0.35185286273451055 1106 14-15 -0.13224386433496704 1106 16-17 -0.27785621541023886 1106 18-19 -0.03408896940858597 1106 20-21 0.014563210173051289 1106 22-23 0.07736628295873516 1106 24-25 -0.1012392239284381 1106 26-27 -0.0194995205789823 1106 28-29 -0.034665635538019046 1106 30-31 -0.03772543718640975 1106 32-33 0.0499628329279318 1106 34-35 -0.2653420784904412 1106 36-37 0.11171953250931921 1106 38-39 0.06865894969039488 1106 40-41 -0.15890141705540373 1106 42-43 -0.09973442527721943 1106 44-45 0.05170741946054136 1106 46-47 -0.11194721880890057 1106 48-49 -0.13872678556545992 1106 50-51 0.12804835372650558 1106 52-53 -0.044691256748564 1106 54-55 -0.005003900703322017 1106 56-57 0.08203480521705586 1106 58-59 0.3777308319249286 1106 60-61 0.26367349735696877 1106 62-63 -0.17620712411558515 1106 64-65 -0.2391712278048601 1106 66-67 -0.2399314695272068 1106 68-69 0.32273801050290984 1106 70-71 0.19793045834692435 1106 72-73 -0.028852757383141636 1106 74-75 -0.10489705163479712 1106 76-77 -0.18790171067741568 1106 78-79 -0.12605104177855964 1106 80-81 -0.47646748135812445 1106 82-83 -0.5078767279213245 1106 84-85 -0.6626190296707968 1106 86-87 -0.6680111786928897 1106 88-89 -0.5702927490945591 1106 90-91 -0.6329421569052478 1106 92-93 -0.7545342980725387 1106 94-95 -0.47995252670384403 1106 96-97 -0.5813183776179365 1106 98-99 -1.0693440476386051 1106 100-101 -1.2044735370133566 1107 1 0.10870550153522629 1107 2 0.06946043658975043 1107 3 0.08645656038996208 1107 4 0.05140102849347272 1107 5 -0.05095417168439553 1107 6 -0.03472062623676919 1107 7 0.058265736346690744 1107 8 0.017873237239840023 1107 9 -0.1331084734391368 1107 10-11 -0.04582134256072834 1107 12-13 -0.0020477101040725643 1107 14-15 0.03667597561756253 1107 16-17 -0.07178307147175644 1107 18-19 0.0033048868854663738 1107 20-21 -0.06498342891028841 1107 22-23 -0.0927523093291569 1107 24-25 -0.13387615832981936 1107 26-27 0.032758463753047806 1107 28-29 -0.03177299403312617 1107 30-31 0.04000102276563666 1107 32-33 -0.016583958505201224 1107 34-35 0.042470659556116175 1107 36-37 -0.09336133315824924 1107 38-39 -0.04146589695751146 1107 40-41 0.08314832916191506 1107 42-43 -0.04482836095353804 1107 44-45 0.0702259379790604 1107 46-47 -0.13307973907542703 1107 48-49 0.020562943652819854 1107 50-51 -0.06349944000790941 1107 52-53 -0.02587004324234954 1107 54-55 -0.12711025689714717 1107 56-57 -0.2932668874693505 1107 58-59 -0.23001455147535665 1107 60-61 -0.18259714002646632 1107 62-63 -0.318822569177442 1107 64-65 -0.0929515073266387 1107 66-67 -0.046703818841230316 1107 68-69 0.12910142089316423 1107 70-71 0.18383810229743602 1107 72-73 0.25158713260427135 1107 74-75 -0.01357738167256528 1107 76-77 -0.10534014933256586 1107 78-79 -0.29058681591597946 1107 80-81 -0.08614329703090462 1107 82-83 -0.08835149799341124 1107 84-85 0.03317451230157076 1107 86-87 -0.03103668525595893 1107 88-89 -0.19206834586474386 1107 90-91 -0.3767519441768208 1107 92-93 -0.0902450900509244 1107 94-95 -0.4831157895553524 1107 96-97 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82-83 -0.04192478959487289 2316 84-85 0.22175882582909168 2316 86-87 0.3509782237921186 2316 88-89 0.3883412322731381 2316 90-91 0.4129480238511789 2316 92-93 0.8358911779753946 2316 94-95 1.19198138849503 2316 96-97 1.0979650035039903 2316 98-99 0.9464445692228907 2316 100-101 1.103017194099646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 11.0 5 21.0 6 35.0 7 86.0 8 198.0 9 390.0 10 721.0 11 1120.0 12 1661.0 13 2421.0 14 3366.0 15 4534.0 16 6124.0 17 8682.0 18 7789.0 19 4711.0 20 4112.0 21 4132.0 22 4296.0 23 4667.0 24 5428.0 25 6363.0 26 8047.0 27 9829.0 28 12236.0 29 15486.0 30 19082.0 31 24501.0 32 31602.0 33 41504.0 34 57410.0 35 83098.0 36 129255.0 37 196056.0 38 151263.0 39 12388.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3243976532245 20.042204855877372 15.531618857686153 22.101778633211975 2 15.50815529253562 20.692881072997693 40.455815355366724 23.343148279099964 3 19.268747898867417 24.738648087824444 30.248194475035646 25.744409538272496 4 12.88397111160057 17.39390004984756 37.619837010073844 32.10229182847802 5 13.679504575026963 37.70239710538218 34.215751081422724 14.402347238168133 6 31.589905289637503 36.891367098292434 17.352283134136304 14.166444477933759 7 28.213022964654606 33.32390480275437 20.471697019579658 17.991375213011374 8 26.011615640541137 35.93626467894694 19.440316242189585 18.61180343832234 9 26.118266232335998 17.264296395905546 20.65740815877027 35.96002921298819 10-11 24.750008694341723 27.427981869399392 27.067630386144696 20.754379050114185 12-13 25.716092548324944 24.506664749976235 28.01942503924192 21.757817662456905 14-15 22.53051713944565 25.954522796563996 26.610539860658683 24.90442020333167 16-17 21.741882382945178 29.396033061683458 27.178917960191505 21.683166595179856 18-19 21.52869712391176 27.508433511470738 30.077727414998318 20.885141949619186 20-21 22.37778653652203 26.744258836349307 30.209011975006668 20.668942652122 22-23 21.989122754749594 26.711328715266692 30.022785063987715 21.276763465996 24-25 22.01388550774032 26.863032183124552 30.101921342505328 21.021160966629804 26-27 21.926096122222923 27.087925686904036 29.920805267206145 21.065172923666896 28-29 21.52150249066508 27.26626177660873 30.10371878264035 21.108516950085843 30-31 21.749881177329794 26.939939487381615 30.105491346231872 21.204687989056723 32-33 21.62219027856671 27.022072035519283 29.944703986645493 21.41103369926852 34-35 21.98601949851037 27.057486987468554 29.82025897545877 21.136234538562306 36-37 22.010421617611257 26.97228243858897 29.74177805084451 21.275517892955264 38-39 21.901162723299677 27.14164821534146 29.845762377844498 21.11142668351437 40-41 22.12016317597293 27.00796520396417 29.673485759198353 21.198385860864548 42-43 22.023412708994428 27.005308843938074 29.482634086279436 21.488644360788058 44-45 22.022014073241134 27.392335068337527 29.190382898809453 21.395267959611886 46-47 22.017435053267334 27.663830379189225 28.603167058878082 21.71556750866536 48-49 22.341965848625716 27.565468393169724 28.58942999895668 21.503135759247883 50-51 22.291828478026503 27.76317772393726 28.258929088948914 21.686064709087326 52-53 22.427170397505304 27.936311048769465 28.055771304035332 21.580747249689903 54-55 22.172857624772643 27.873744100882302 28.242674428983953 21.7107238453611 56-57 22.31315859638547 28.16271170722094 28.05606111542608 21.468068580967508 58-59 22.362658381925044 27.8249655124445 28.20757451050856 21.604801595121895 60-61 22.420504735518122 27.5956087778074 28.545958290344643 21.43792819632983 62-63 22.287365382608996 27.7966799207076 28.506370054368617 21.409584642314783 64-65 22.31207024568485 27.6162013935299 28.406285664934945 21.665442695850302 66-67 22.225171858154713 27.97033490604315 28.10370610806487 21.70078712773727 68-69 22.190336528986933 27.95868448813512 28.202357905475118 21.648621077402826 70-71 22.298030441788484 27.88843420701807 28.065914702711474 21.74762064848197 72-73 22.447283308023138 27.763873271275052 27.987491740375365 21.801351680326444 74-75 22.302087801258942 27.94129580469031 27.756454099671934 22.000162294378818 76-77 22.205000985358726 28.04174443272318 27.86919073067248 21.884063851245607 78-79 22.315882823458494 27.92164659239767 27.818821510960667 21.94364907318317 80-81 22.125998400241123 28.184795335195854 27.810185131516405 21.879021133046614 82-83 22.21922750104622 28.136835186389515 27.63389591585722 22.01004139670704 84-85 22.370251440362612 27.945237239604463 27.615489839212643 22.069021480820282 86-87 22.351297775407765 27.796216222482407 27.73813801977673 22.114347982333097 88-89 22.2545007708983 27.84936763154539 27.789028899991884 22.107102697564425 90-91 22.227233334589332 27.930877566222527 27.87998063829849 21.961908460889653 92-93 22.259447208947137 27.67960492567722 27.878649119400436 22.18229874597521 94-95 22.108667679074458 27.805722036098906 27.834297439226553 22.251312845600083 96-97 22.2498058263682 27.837485364524767 27.665743134368153 22.24696567473888 98-99 22.17194281633713 27.816464647401418 27.50850892161591 22.503083614645547 100-101 22.251756140913315 27.69482192786213 27.703401901623643 22.35002002960091 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 210.0 1 141.5 2 91.0 3 112.0 4 107.0 5 112.5 6 201.5 7 478.5 8 750.5 9 866.0 10 859.0 11 841.5 12 896.0 13 1028.0 14 1203.5 15 1326.5 16 1405.0 17 1449.5 18 1497.0 19 1603.0 20 1755.0 21 1959.0 22 2354.5 23 2948.5 24 3645.0 25 4345.0 26 5472.0 27 6892.0 28 8076.0 29 9508.0 30 11055.0 31 12472.0 32 14019.5 33 15757.0 34 17976.5 35 19857.0 36 21642.5 37 23514.0 38 25031.5 39 26082.0 40 26982.0 41 28683.0 42 30451.5 43 31688.5 44 32586.5 45 33127.0 46 32925.5 47 33290.0 48 35154.0 49 35095.0 50 33520.0 51 31937.0 52 29809.0 53 27670.5 54 25634.0 55 23623.0 56 21221.0 57 18632.5 58 16389.5 59 14660.5 60 12379.5 61 9612.5 62 7227.5 63 5450.0 64 4099.5 65 3051.0 66 2209.0 67 1724.0 68 1272.0 69 778.5 70 574.0 71 435.0 72 341.5 73 279.5 74 208.5 75 155.0 76 109.5 77 78.5 78 42.5 79 27.5 80 22.0 81 14.5 82 7.5 83 5.0 84 5.0 85 2.5 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01970717457078933 2 0.0 3 0.0 4 0.0 5 0.03941434914157866 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0037095858015603446 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.057359470456627E-4 24-25 4.636982251950431E-4 26-27 0.0011012832848382272 28-29 0.0010433210066888468 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.477736688962823E-4 42-43 0.012403927523967401 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.1592455629876077E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.7962278149380385E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.1592455629876077E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011882267020622977 92-93 8.694341722407058E-4 94-95 0.0 96-97 0.0 98-99 6.955473377925646E-4 100-101 0.01814219306075606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 862630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.23616930736831 #Duplication Level Percentage of deduplicated Percentage of total 1 81.88316950826652 55.055106484817465 2 11.402007990954969 15.332546794476295 3 3.184687785565811 6.423786214242323 4 1.2371282170478919 3.327190490254191 5 0.636852535570445 2.140976245272062 6 0.37554818838062226 1.5150252946220983 7 0.2555488495572483 1.202748800059404 8 0.18181464070426373 0.9779615971960173 9 0.12812605885393724 0.7753234847210246 >10 0.6523193359134186 7.986498209004421 >50 0.04485693397258197 2.0508281141478157 >100 0.016904957836631297 1.9126608276409398 >500 5.174986878211135E-4 0.21549473802628638 >1k 5.174986878211135E-4 1.0838527055197233 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3932 0.4558153553667273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3187 0.36945156092415055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2226 0.2580480623210415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.3184911259752154E-4 0.0 5 0.0 0.0 0.0 2.3184911259752154E-4 0.0 6 0.0 0.0 0.0 2.3184911259752154E-4 0.0 7 0.0 0.0 0.0 2.3184911259752154E-4 0.0 8 0.0 0.0 0.0 2.3184911259752154E-4 0.0 9 0.0 0.0 0.0 2.3184911259752154E-4 0.0 10-11 0.0 0.0 0.0 2.3184911259752154E-4 0.0 12-13 0.0 0.0 0.0 2.3184911259752154E-4 0.0 14-15 0.0 0.0 0.0 2.898113907469019E-4 0.0 16-17 0.0 0.0 0.0 5.796227814938038E-4 0.0 18-19 0.0 0.0 0.0 5.796227814938038E-4 0.0 20-21 0.0 0.0 0.0 5.796227814938038E-4 0.0 22-23 0.0 0.0 0.0 5.796227814938038E-4 0.0 24-25 0.0 0.0 0.0 5.796227814938038E-4 0.0 26-27 0.0 0.0 0.0 6.375850596431842E-4 0.0 28-29 0.0 0.0 0.0 9.273964503900862E-4 0.0 30-31 0.0 0.0 0.0 0.001970717457078933 0.0 32-33 0.0 0.0 0.0 0.0035936612452615838 1.1592455629876077E-4 34-35 0.0 0.0 0.0 0.00457901997380105 1.1592455629876077E-4 36-37 0.0 0.0 0.0 0.006955473377925646 1.1592455629876077E-4 38-39 0.0 0.0 0.0 0.00892619083500458 1.1592455629876077E-4 40-41 0.0 0.0 0.0 0.011592455629876076 1.1592455629876077E-4 42-43 0.0 0.0 0.0 0.017330721166664734 1.1592455629876077E-4 44-45 0.0 0.0 0.0 0.026546723392416215 1.1592455629876077E-4 46-47 0.0 0.0 0.0 0.03790732990969477 1.1592455629876077E-4 48-49 0.0 0.0 0.0 0.05999095788460869 1.1592455629876077E-4 50-51 0.0 0.0 0.0 0.07992998156799555 1.1592455629876077E-4 52-53 0.0 0.0 0.0 0.10317285510589708 1.1592455629876077E-4 54-55 0.0 0.0 0.0 0.1429929401945214 1.1592455629876077E-4 56-57 0.0 0.0 0.0 0.1825811761705482 1.1592455629876077E-4 58-59 0.0 0.0 0.0 0.23097967842528083 1.1592455629876077E-4 60-61 0.0 0.0 0.0 0.28233425686563185 1.1592455629876077E-4 62-63 0.0 0.0 0.0 0.3485851407903736 1.1592455629876077E-4 64-65 0.0 0.0 0.0 0.4009830402374135 1.1592455629876077E-4 66-67 0.0 0.0 0.0 0.47871045523573263 1.1592455629876077E-4 68-69 0.0 0.0 0.0 0.5813036875601358 1.1592455629876077E-4 70-71 0.0 0.0 0.0 0.6584514797769612 1.1592455629876077E-4 72-73 0.0 0.0 0.0 0.7791869051621205 1.1592455629876077E-4 74-75 0.0 0.0 0.0 0.9288455073438207 1.1592455629876077E-4 76-77 0.0 0.0 0.0 1.054449764093528 1.1592455629876077E-4 78-79 0.0 0.0 0.0 1.1790106998365464 1.1592455629876077E-4 80-81 0.0 0.0 0.0 1.3542886289602727 1.1592455629876077E-4 82-83 0.0 0.0 0.0 1.5634744908013865 1.1592455629876077E-4 84-85 0.0 0.0 1.1592455629876077E-4 1.77550050427182 1.1592455629876077E-4 86-87 0.0 0.0 1.1592455629876077E-4 2.0716877456151535 1.1592455629876077E-4 88-89 0.0 0.0 1.1592455629876077E-4 2.4254315291608224 1.1592455629876077E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2535 0.0 43.866657 1 GTATCAA 4145 0.0 35.88529 1 ATCAACG 5085 0.0 28.684238 3 TATCAAC 5195 0.0 27.985416 2 TCAACGC 5165 0.0 27.963991 4 CAACGCA 5215 0.0 27.69588 5 AACGCAG 5365 0.0 26.91373 6 ACGCAGA 6120 0.0 23.13051 7 CGCAGAG 6100 0.0 23.0506 8 GTACATG 3400 0.0 22.92248 1 TACATGG 3460 0.0 21.970491 2 GAGTACT 3725 0.0 21.934238 12-13 GCAGAGT 6480 0.0 21.772177 9 GTATAGC 350 0.0 21.72444 1 GTACACG 110 0.0028689958 21.601006 1 GTACTTT 3860 0.0 21.287704 14-15 ACATGGG 3475 0.0 20.645151 3 AGTACTT 3885 0.0 20.419535 12-13 GGGCGTA 140 4.8483704E-4 20.358398 9 GTACTAC 240 1.4129728E-7 19.790592 6 >>END_MODULE