FastQCFastQC Report
Thu 26 May 2016
SRR937754_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937754_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737262
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30760.41721938740908926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22950.3112868966527503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15370.20847405671253907No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10620.14404648550990015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.044.925551
GTATCAA29150.040.9592631
TATCAAC37300.032.119752
ATCAACG37200.031.9461423
TCAACGC37600.031.606294
CAACGCA37950.031.3126665
AACGCAG39550.030.0316036
ACGCAGA44550.026.5544097
CGCAGAG44450.026.4003818
GTACATG27750.024.3412191
TACATGG28700.023.688342
GCAGAGT48450.023.6308169
GTGTAGG2903.6379788E-1222.963971
TAGCCCT5450.022.6776584
ACATGGG29250.022.264663
AGTACTT27900.022.21486512-13
AGAGTAC43650.021.70754810-11
GTATAGC3301.8189894E-1221.6219221
GAGTACT26300.021.30902712-13
GTGTAGC4800.019.8200931
Produced by FastQC (version 0.11.5)