FastQCFastQC Report
Thu 26 May 2016
SRR937754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737262
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33930.4602163138748504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29080.3944323727521559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.2632714014827836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.038.5061231
GTATCAA36250.038.409721
ATACGCC550.002575273934.5557173
ACCGTCG753.076922E-431.667488
ATCAACG46100.029.7865033
TATCAAC46950.029.5508422
TCAACGC46650.029.5371784
CAACGCA47350.029.1005125
AACGCAG48100.028.6370476
ACGCAGA55200.024.8692977
CGCAGAG55500.024.7348698
GTACATG32100.024.7225341
TGAACCG1351.3387933E-524.6369465
CCTACGT1551.4058478E-624.5234132
GTAAGGT2751.8189894E-1224.1890034
TACATGG32650.024.0117242
ACATGGG32300.023.8306063
CATACGC1000.001650229323.7570552
ACGTCTA1601.8532537E-623.7570555
GCAGAGT59400.023.0308959
Produced by FastQC (version 0.11.5)