FastQCFastQC Report
Thu 26 May 2016
SRR937753_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937753_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661010
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28960.43811742636268736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.3263188151465182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.21482277121374865No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9650.1459887142403292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.041.0250131
GTATCAA30250.035.0632481
ATCAACG36600.028.7003443
TCAACGC36750.028.4538634
TATCAAC36900.028.344652
CAACGCA37300.028.030055
AACGCAG38300.027.4118776
AACCGCT700.00838415527.1460867
TAGCCCT4950.025.9259414
GTGTAGC5000.025.684221
GTACATG25650.025.5896511
TACATGG25300.025.1801972
ACGCAGA42600.024.421927
ACATGGG25600.024.3224453
CGCAGAG42800.024.1968028
GAGTACT24900.023.55846412-13
GCAGAGT45100.022.6468189
GTACTTT26550.022.0032614-15
AGTACTT26350.021.4509112-13
GTGTATA3950.020.4703121
Produced by FastQC (version 0.11.5)