##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937753_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 661010 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74523229603183 33.0 31.0 34.0 30.0 34.0 2 31.89273687236199 33.0 31.0 34.0 30.0 34.0 3 31.917420311341736 34.0 31.0 34.0 30.0 34.0 4 35.49986838323172 37.0 35.0 37.0 33.0 37.0 5 35.483656828187165 37.0 35.0 37.0 33.0 37.0 6 35.52598599113478 37.0 35.0 37.0 33.0 37.0 7 35.467486119725876 37.0 35.0 37.0 33.0 37.0 8 35.45407331205277 37.0 35.0 37.0 33.0 37.0 9 37.09565210813755 39.0 37.0 39.0 33.0 39.0 10-11 36.98399040861712 39.0 37.0 39.0 33.0 39.0 12-13 36.859542215700216 39.0 37.0 39.0 33.0 39.0 14-15 38.0237182493457 40.0 38.0 41.0 33.0 41.0 16-17 37.96218438450251 40.0 38.0 41.0 33.0 41.0 18-19 37.90296062086807 40.0 38.0 41.0 33.0 41.0 20-21 37.8211638250556 40.0 38.0 41.0 33.0 41.0 22-23 37.62959486240753 40.0 37.5 41.0 32.0 41.0 24-25 37.462239603031726 40.0 37.0 41.0 32.0 41.0 26-27 37.408843285275566 40.0 37.0 41.0 32.0 41.0 28-29 37.44187682485893 40.0 37.0 41.0 32.0 41.0 30-31 37.27042631730231 40.0 37.0 41.0 31.5 41.0 32-33 37.03606980227228 39.0 36.0 41.0 31.0 41.0 34-35 37.076171313595864 39.5 36.0 41.0 31.0 41.0 36-37 36.983929138742226 40.0 36.0 41.0 31.0 41.0 38-39 36.85451581670474 40.0 36.0 41.0 31.0 41.0 40-41 36.61627206850123 39.5 36.0 41.0 30.0 41.0 42-43 36.38025370266713 39.0 36.0 41.0 30.0 41.0 44-45 36.15085777824844 39.0 35.0 41.0 29.5 41.0 46-47 35.80978578236335 39.0 35.0 41.0 28.5 41.0 48-49 35.45592880591822 39.0 35.0 40.5 26.5 41.0 50-51 34.53053357740427 38.0 34.0 39.5 25.0 40.5 52-53 34.62860168529977 38.0 34.5 39.5 25.5 40.5 54-55 34.98502518872634 39.0 35.0 40.0 26.0 41.0 56-57 34.780563834132614 38.0 34.0 40.0 24.5 41.0 58-59 34.545558312279695 38.0 34.0 40.0 24.0 41.0 60-61 34.24362339450235 38.0 34.0 40.0 23.0 41.0 62-63 33.89922240208166 38.0 33.5 40.0 22.0 41.0 64-65 33.52401552170164 37.0 33.0 40.0 21.5 41.0 66-67 33.1219648719384 36.5 32.5 39.5 20.0 41.0 68-69 32.64441309511203 36.0 32.0 39.0 18.0 40.5 70-71 32.21349525725783 35.5 31.5 39.0 16.5 40.0 72-73 31.755915190390464 35.0 31.0 38.0 12.0 40.0 74-75 31.28853799488661 35.0 31.0 37.5 8.0 39.5 76-77 30.78761667750866 35.0 30.5 37.0 7.0 39.0 78-79 30.247897157380372 34.0 30.0 36.0 2.0 39.0 80-81 29.720447497012145 34.0 29.0 36.0 2.0 37.5 82-83 29.188637085671928 34.0 29.0 35.0 2.0 37.0 84-85 28.72054658779746 34.0 29.0 35.0 2.0 36.5 86-87 28.240258846310947 34.0 27.5 35.0 2.0 36.0 88-89 27.76374865735768 33.5 26.5 35.0 2.0 36.0 90-91 27.270170647947836 33.0 26.0 35.0 2.0 35.0 92-93 26.708698052979532 33.0 25.0 35.0 2.0 35.0 94-95 26.0565770563229 32.0 24.5 35.0 2.0 35.0 96-97 25.32120315880244 32.0 21.5 34.0 2.0 35.0 98-99 24.372615391597705 32.0 9.5 34.0 2.0 35.0 100-101 21.987892770154765 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.03566498624512704 1101 2 0.012817406809567444 1101 3 -0.08057529306818623 1101 4 -0.05553359808346414 1101 5 -0.07294768013395014 1101 6 0.06135553032906671 1101 7 -0.16236900717364477 1101 8 -0.1414139796881244 1101 9 0.0014762369718397395 1101 10-11 -0.11217509146150206 1101 12-13 -0.01715921994899361 1101 14-15 0.0759656920375349 1101 16-17 0.24864973769964394 1101 18-19 0.20534683973595946 1101 20-21 0.2027660535312421 1101 22-23 0.26527407535213854 1101 24-25 0.16337207036755075 1101 26-27 0.22373355742313805 1101 28-29 -0.044073641374154704 1101 30-31 0.1736311698060149 1101 32-33 0.13072035873619825 1101 34-35 0.32190551629651765 1101 36-37 0.06987663592295235 1101 38-39 -0.010641501628413153 1101 40-41 0.14914997372342498 1101 42-43 0.09792281671902714 1101 44-45 -0.006070127676558457 1101 46-47 0.16701742404676168 1101 48-49 0.2582675693066747 1101 50-51 0.3192142040002608 1101 52-53 0.2617988259045845 1101 54-55 0.18553336058140246 1101 56-57 0.26676557537452794 1101 58-59 0.43987901306550015 1101 60-61 0.27663271431378433 1101 62-63 0.4305751022532931 1101 64-65 0.26579365387719633 1101 66-67 0.3567730200657877 1101 68-69 0.12933301759072435 1101 70-71 0.3925184675850275 1101 72-73 0.40608444483094175 1101 74-75 0.4285535383403065 1101 76-77 0.6486823689070711 1101 78-79 0.5639612048170655 1101 80-81 0.30022033920805313 1101 82-83 0.29140823543765393 1101 84-85 0.6024542968545212 1101 86-87 0.6886602458970863 1101 88-89 0.584683256561874 1101 90-91 0.6859828505042351 1101 92-93 0.6587348335936198 1101 94-95 0.7974043574069789 1101 96-97 0.9577112691173717 1101 98-99 1.4658121644200648 1101 100-101 1.9031570925066568 1102 1 0.08777544237709733 1102 2 -0.1624554541473593 1102 3 0.11517223445897784 1102 4 0.05440209652923045 1102 5 -0.07047128991415974 1102 6 0.020857600516563934 1102 7 -0.016896600633415915 1102 8 0.031054636028422067 1102 9 0.1007078267673549 1102 10-11 -0.12116428550466196 1102 12-13 -0.0443570238024904 1102 14-15 0.11144725923274734 1102 16-17 0.19462628160147943 1102 18-19 0.028202673928106492 1102 20-21 0.10971097509312955 1102 22-23 0.20604561946490918 1102 24-25 0.18652652985877438 1102 26-27 0.17951531687156574 1102 28-29 0.015456151848162847 1102 30-31 0.3171032991158924 1102 32-33 0.21337839510159284 1102 34-35 0.2723972988273857 1102 36-37 0.4253977385765353 1102 38-39 0.4770461561970194 1102 40-41 0.43458001223433484 1102 42-43 0.5138231825425521 1102 44-45 0.4449056026144689 1102 46-47 0.4910246681963031 1102 48-49 0.5136054588605106 1102 50-51 0.6444795315238139 1102 52-53 0.4883547812343423 1102 54-55 0.5938255613684902 1102 56-57 0.4126981240254466 1102 58-59 0.41891919338575434 1102 60-61 0.47749122438145974 1102 62-63 0.5360769193174164 1102 64-65 0.45311126945512825 1102 66-67 0.724071892461474 1102 68-69 0.7863541774983958 1102 70-71 0.7597188026721469 1102 72-73 0.8827880554548919 1102 74-75 0.7373097986234463 1102 76-77 0.8001531964396627 1102 78-79 0.7495925366678193 1102 80-81 0.6140854726703573 1102 82-83 0.6493563089880716 1102 84-85 0.9963226847600488 1102 86-87 1.0134437350218235 1102 88-89 0.9851379746005264 1102 90-91 1.0054814854461078 1102 92-93 0.8985131698499274 1102 94-95 1.1058948381605624 1102 96-97 1.0753732530018496 1102 98-99 0.7449978906731758 1102 100-101 1.1808475829030343 1103 1 0.05776868672214874 1103 2 0.13806636195905853 1103 3 0.11063429796213953 1103 4 0.11901644181308058 1103 5 0.24995874246639715 1103 6 0.13229562320624666 1103 7 0.09348148309010895 1103 8 0.08635316609108656 1103 9 0.09005718730966805 1103 10-11 0.16985418771731986 1103 12-13 0.09790425697650562 1103 14-15 0.16868745770923255 1103 16-17 0.2135991890956035 1103 18-19 0.07692492332825651 1103 20-21 -0.03205644401372609 1103 22-23 0.10297762030354107 1103 24-25 0.028235710560899463 1103 26-27 0.11337978445607888 1103 28-29 -0.08452987093470199 1103 30-31 0.1155192145071382 1103 32-33 0.11520708101021881 1103 34-35 0.36437321142319234 1103 36-37 0.2938127221533051 1103 38-39 0.5685841159892249 1103 40-41 0.45609211417484374 1103 42-43 0.5125396081022231 1103 44-45 0.4981110444110115 1103 46-47 0.36534502601305974 1103 48-49 0.6320751865377261 1103 50-51 0.7633487746575014 1103 52-53 0.5720608403579703 1103 54-55 0.6396778221833657 1103 56-57 0.563275849000398 1103 58-59 0.35724006370048045 1103 60-61 0.6107558363972103 1103 62-63 0.67654562199872 1103 64-65 0.7084389187201623 1103 66-67 0.6652441150719568 1103 68-69 0.8072489523487292 1103 70-71 1.0004076465233354 1103 72-73 0.6357989809796187 1103 74-75 0.7801150261026528 1103 76-77 0.8430259804683651 1103 78-79 1.0903279712427967 1103 80-81 0.6128811024604666 1103 82-83 0.9042949257094826 1103 84-85 0.8494011668342658 1103 86-87 0.8872795493111987 1103 88-89 0.8679448327550716 1103 90-91 1.0224300259916852 1103 92-93 0.935652965316649 1103 94-95 1.1835406088876113 1103 96-97 1.1372736886251182 1103 98-99 1.284612818341074 1103 100-101 1.962445411775306 1104 1 0.3244279197509279 1104 2 0.3034231315252889 1104 3 0.27913910054273217 1104 4 0.2547434065461047 1104 5 0.1559648966175189 1104 6 0.20326095992751192 1104 7 0.2825875993972602 1104 8 0.28736887134058975 1104 9 0.3453350702096003 1104 10-11 0.4573715328723864 1104 12-13 0.47162702283041824 1104 14-15 0.5059076826105695 1104 16-17 0.5225034483448425 1104 18-19 0.42135634013457945 1104 20-21 0.4966970925958165 1104 22-23 0.48160264508233297 1104 24-25 0.16624063478284512 1104 26-27 0.3005221814213499 1104 28-29 0.3686477840003306 1104 30-31 0.4870411730229378 1104 32-33 0.4328436121594237 1104 34-35 0.5409632215149145 1104 36-37 0.7902197971744727 1104 38-39 0.5942842295211506 1104 40-41 0.7755774811712897 1104 42-43 0.6998064621866646 1104 44-45 0.767131020623367 1104 46-47 0.8693073802687081 1104 48-49 0.8030775788175575 1104 50-51 0.859786710538998 1104 52-53 0.7846809048729355 1104 54-55 0.9447334843628141 1104 56-57 0.8416577037155761 1104 58-59 0.8111869875259643 1104 60-61 0.8676133673481772 1104 62-63 0.8850788751388663 1104 64-65 0.8920148589627246 1104 66-67 0.8386590607388271 1104 68-69 1.0696884695677369 1104 70-71 1.01300822589657 1104 72-73 1.004264700645873 1104 74-75 1.0681887677904918 1104 76-77 1.251850159164011 1104 78-79 1.2057639203156185 1104 80-81 1.4296064498140915 1104 82-83 1.0695846799038584 1104 84-85 1.4013138643888254 1104 86-87 1.4189631781879548 1104 88-89 1.2725718218163564 1104 90-91 1.2916555535547118 1104 92-93 1.4246061288927265 1104 94-95 1.436526453438983 1104 96-97 1.5123018463443891 1104 98-99 1.8990868997236419 1104 100-101 2.493757921912085 1105 1 -0.07462388584153601 1105 2 0.05325888609463547 1105 3 0.0027786316497113717 1105 4 -0.08312605340015722 1105 5 -0.17697535495480565 1105 6 -0.13640412703028915 1105 7 -0.11196129775926522 1105 8 -0.27698765566209715 1105 9 -0.14752302173831566 1105 10-11 -0.20850158845515665 1105 12-13 -0.17501277402196536 1105 14-15 -0.25860312436967803 1105 16-17 -0.11870056293457054 1105 18-19 -0.17884721347217436 1105 20-21 0.08852505196629323 1105 22-23 -0.005996824903199638 1105 24-25 -0.18223372816633798 1105 26-27 -0.2499094703340461 1105 28-29 -0.2671195189789728 1105 30-31 -0.10987101288075252 1105 32-33 -0.21348522564729677 1105 34-35 -0.20408081102413433 1105 36-37 -0.09316594706032788 1105 38-39 0.2734372398261655 1105 40-41 0.29032450629899387 1105 42-43 0.003490727105322833 1105 44-45 -0.09852544437205779 1105 46-47 -0.06677715630081593 1105 48-49 -0.24593307314452062 1105 50-51 -0.5315806234439364 1105 52-53 -0.4404497532898759 1105 54-55 -0.2396375479104904 1105 56-57 -0.2323284037046207 1105 58-59 -0.4135515116029822 1105 60-61 -0.6608518421477996 1105 62-63 -0.5937639143021869 1105 64-65 -0.4245119222697511 1105 66-67 -0.41173863465787264 1105 68-69 0.03280060675142238 1105 70-71 0.09472586401036409 1105 72-73 -0.019498291352238795 1105 74-75 3.351049328443878E-4 1105 76-77 0.14538774737672 1105 78-79 0.048888311315707256 1105 80-81 0.018036005133431843 1105 82-83 0.10942157865485314 1105 84-85 0.3224114667746285 1105 86-87 0.317274033888193 1105 88-89 0.056390694169976996 1105 90-91 -0.11897555489894529 1105 92-93 0.040087275731831795 1105 94-95 0.43660211706433927 1105 96-97 -0.20842002155061223 1105 98-99 0.1161704545114901 1105 100-101 0.4585890997548212 1106 1 0.05521469135522494 1106 2 0.0612565343537419 1106 3 0.12154154677088869 1106 4 0.2102795613812276 1106 5 0.24107252718147265 1106 6 0.301650844913091 1106 7 0.031425727893733324 1106 8 0.03937337114948036 1106 9 0.056248409797497345 1106 10-11 0.14757532730583023 1106 12-13 0.206963552939186 1106 14-15 0.39208553799122825 1106 16-17 0.22620558358234888 1106 18-19 0.40373607014604573 1106 20-21 0.49389774237516804 1106 22-23 0.5006779691033287 1106 24-25 0.6384849361831684 1106 26-27 0.2728989578902983 1106 28-29 0.4283430044735894 1106 30-31 0.4828671224999823 1106 32-33 0.5104273103421235 1106 34-35 0.45454929990540904 1106 36-37 0.6281354648488247 1106 38-39 0.6249619806591298 1106 40-41 0.4420161169821242 1106 42-43 0.26945360072519264 1106 44-45 0.0742929268744561 1106 46-47 0.12878912042646107 1106 48-49 0.2337442541711212 1106 50-51 0.46309337512080617 1106 52-53 0.2987015449229702 1106 54-55 0.405517606465736 1106 56-57 0.4127273204500028 1106 58-59 0.5033507614583428 1106 60-61 0.3756563413917462 1106 62-63 0.3167488862580896 1106 64-65 0.4013684674967237 1106 66-67 0.47149137770605165 1106 68-69 0.5240377780913121 1106 70-71 0.525448937874053 1106 72-73 0.5700494308310731 1106 74-75 0.49749485903758384 1106 76-77 0.7857616050099345 1106 78-79 0.6938835127267531 1106 80-81 0.9679102042878398 1106 82-83 0.696418699501482 1106 84-85 0.7300018834304858 1106 86-87 0.6866616301842008 1106 88-89 0.8136988154575562 1106 90-91 1.2726714418760565 1106 92-93 1.3388491533202576 1106 94-95 1.4070626951430008 1106 96-97 1.4400725298288748 1106 98-99 1.7108582868461966 1106 100-101 2.046537292286313 1107 1 -0.05778746240449095 1107 2 0.08482303558669102 1107 3 -0.08422834429256909 1107 4 -0.2502933699169603 1107 5 -0.10564767133898556 1107 6 -0.2506285507856276 1107 7 -0.1885308847728666 1107 8 0.05753596440103337 1107 9 -0.09742156554352022 1107 10-11 -0.09111743418868912 1107 12-13 -0.11046044119260046 1107 14-15 0.08989393141133206 1107 16-17 -0.04088598241946073 1107 18-19 -0.0018028379914198922 1107 20-21 0.05146204302753432 1107 22-23 0.049469210480474146 1107 24-25 -0.10293709147235575 1107 26-27 -0.08901851253451554 1107 28-29 -0.37464650765301144 1107 30-31 -0.24459058265343714 1107 32-33 -0.2320208745690735 1107 34-35 -0.26217277144958473 1107 36-37 -0.13824598472510985 1107 38-39 0.12534435104337405 1107 40-41 0.3333605000392126 1107 42-43 0.44846731923815497 1107 44-45 0.20469098224793214 1107 46-47 0.019671147457700044 1107 48-49 0.29601210072353723 1107 50-51 0.2171180185018997 1107 52-53 0.33608800432929087 1107 54-55 0.20053976834107345 1107 56-57 0.13840778366441242 1107 58-59 0.20530475215660005 1107 60-61 0.40621865996090634 1107 62-63 0.3333020123310604 1107 64-65 0.29843475023231036 1107 66-67 0.24794711638320877 1107 68-69 0.27542679703815054 1107 70-71 0.3503623192768046 1107 72-73 0.48264853499189186 1107 74-75 0.6386636638570131 1107 76-77 0.45847791739873855 1107 78-79 0.7351151355378782 1107 80-81 0.7354407158838079 1107 82-83 0.7963695375949342 1107 84-85 0.8934788787796144 1107 86-87 1.109811035559236 1107 88-89 1.2969662094082999 1107 90-91 1.4769469670500754 1107 92-93 1.1612146994075943 1107 94-95 0.9996060824763973 1107 96-97 1.2884451146526708 1107 98-99 2.011824747501066 1107 100-101 2.4131095368785047 1108 1 0.2893562915189243 1108 2 0.33556848442536236 1108 3 0.24306293003260393 1108 4 0.3348447342889358 1108 5 0.1586727931933396 1108 6 0.21802336384086374 1108 7 0.14182065320578374 1108 8 0.32711380495344855 1108 9 0.17443185567431385 1108 10-11 0.31968372714212734 1108 12-13 0.42253547845585615 1108 14-15 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0.5677232179921177 2316 56-57 0.5283030977222367 2316 58-59 0.5790435358056527 2316 60-61 0.5183472741184261 2316 62-63 0.31421178301899744 2316 64-65 0.3849477113489641 2316 66-67 0.2673813137909846 2316 68-69 0.3989462387131226 2316 70-71 0.5764141637434079 2316 72-73 0.5828454442640769 2316 74-75 0.3730056379347779 2316 76-77 0.3657486053349288 2316 78-79 0.19684743862860543 2316 80-81 0.6306899681270792 2316 82-83 0.0857264668072979 2316 84-85 0.13255415295708062 2316 86-87 0.4659401482212502 2316 88-89 0.6139328096077001 2316 90-91 0.5188961195924477 2316 92-93 0.5934629546418932 2316 94-95 0.3681330017186717 2316 96-97 0.6371714356895595 2316 98-99 1.2411193636526079 2316 100-101 1.4712301749642478 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2861.0 3 505.0 4 497.0 5 592.0 6 681.0 7 758.0 8 916.0 9 1154.0 10 1427.0 11 1845.0 12 2432.0 13 3148.0 14 3674.0 15 4333.0 16 5434.0 17 7074.0 18 6126.0 19 4443.0 20 4249.0 21 4704.0 22 4950.0 23 5465.0 24 5993.0 25 6992.0 26 8029.0 27 9465.0 28 10988.0 29 13291.0 30 16017.0 31 19934.0 32 25498.0 33 33423.0 34 45103.0 35 63875.0 36 92700.0 37 127526.0 38 101822.0 39 13085.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8708903797699 19.081843295589085 15.227224431559454 22.82004189308157 2 14.702115390451912 19.70317763816808 41.84557953200401 23.749127439376004 3 18.793697805399106 24.359250582637404 30.293503592930666 26.553548019032824 4 12.23810224536886 16.524191456341907 38.22740105494866 33.01030524334058 5 13.489795237271732 37.04099805522434 34.85638696484538 14.612819742658548 6 31.258898503610954 36.197027842934496 18.087672720049856 14.456400933404698 7 27.968379734284245 32.82649334346104 21.02453542194467 18.180591500310047 8 25.579201953039117 35.06751480321766 20.28537419349947 19.06790905024375 9 25.59038402634779 17.16473132105913 21.08354081815232 36.161343834440764 10-11 24.31915848595404 26.806352903356895 27.842475412371602 21.032013198317458 12-13 25.419023214204483 24.075601582993432 28.397901472305197 22.107473730496885 14-15 22.265788765441926 25.37778940226147 27.08909029570412 25.267331536592486 16-17 21.560900611723962 28.69901952831526 27.58709049405675 22.152989365904027 18-19 21.18655889278561 27.180002698895727 30.312237975888856 21.32120043242981 20-21 22.09480985801792 26.238069935137435 30.400052757208446 21.267067449636194 22-23 21.590246749358588 26.27806769216776 30.270776250516352 21.860909307957296 24-25 21.502969648644633 26.409787258554985 30.446629698911888 21.640613393888497 26-27 21.362939288890033 26.755279350551 30.1719517658733 21.709829594685665 28-29 21.108413675141097 26.647676784831546 30.51598698356569 21.727922556461664 30-31 21.345518917095323 26.371882687853716 30.43512541270389 21.847472982347078 32-33 21.20188815716649 26.518812577807633 30.173212490465957 22.10608677455992 34-35 21.62007670608107 26.69012274640824 30.02567735684703 21.664123190663663 36-37 21.514793419689237 26.51081773315374 30.057175602549595 21.91721324460743 38-39 21.418695927026974 26.714227890625352 30.062564334236352 21.80451184811132 40-41 21.70811438463573 26.543820435919187 30.031613501261585 21.716451678183503 42-43 21.495582855384185 26.559797085760984 29.791809748191678 22.152810310663153 44-45 21.517282260497538 26.9026101906135 29.564725662402008 22.015381886486953 46-47 21.543197523339973 27.037983741612315 29.097723158651178 22.321095576396534 48-49 21.68817641225934 26.97317548738071 29.09728813456529 22.24135996579465 50-51 21.700301905904222 27.181690946007215 28.780912753591103 22.33709439449746 52-53 21.8380981269432 27.162660195647227 28.69409266702055 22.30514901038902 54-55 21.60937960772244 27.084178279864485 28.899028668419856 22.407413443993214 56-57 21.74855064226441 27.51309158425221 28.623166988746163 22.11519078473722 58-59 21.74661959140308 27.152545224308476 28.862850478444425 22.237984705844017 60-61 21.785053592229183 26.98345814061528 29.12737466663534 22.104113600520197 62-63 21.779262003583565 27.068433387198 28.977243443013105 22.17506116620533 64-65 21.677109668188404 26.946051060063176 29.008430184972987 22.368409086775433 66-67 21.561226717112923 27.308886301901435 28.744209594489718 22.385677386495924 68-69 21.57545615362111 27.261820127603265 28.827226273720974 22.335497445054653 70-71 21.59279856068219 26.99607582791846 28.875479911543188 22.53564569985616 72-73 21.634360325347192 26.990371760397498 28.753982714035825 22.621285200219486 74-75 21.491847039331574 27.045213044130733 28.708547382973144 22.75439253356455 76-77 21.515674820069712 27.089306371965595 28.63601698705654 22.75900182090815 78-79 21.610919897502768 27.115673282489045 28.64744590844996 22.62596091155823 80-81 21.398510329604356 27.301249679734507 28.60816028889842 22.69207970176271 82-83 21.60927468562636 27.204026408143772 28.522159646476513 22.66453925975335 84-85 21.656475289067284 26.99542828039072 28.34625319080563 23.00184323973637 86-87 21.590455176972 27.01592395551388 28.58436801019909 22.80925285731502 88-89 21.629695549215146 26.77429526965771 28.655471230143693 22.940537950983455 90-91 21.48255337986574 26.81697725329436 28.88561760550142 22.814851761338485 92-93 21.630959193805737 26.70780005836495 28.85403836168834 22.80720238614098 94-95 21.346801193673258 26.852797226384816 28.643610308279687 23.156791271662236 96-97 21.298619800567963 26.924916271741118 28.651018191712136 23.125445735978786 98-99 21.354653423470747 26.786925679081403 28.52016835958551 23.338252537862335 100-101 21.569204022441884 26.43408814116288 28.839171290271974 23.157536546123257 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 911.0 1 525.5 2 137.5 3 133.5 4 116.5 5 98.5 6 149.0 7 322.0 8 569.0 9 688.5 10 648.5 11 636.5 12 681.5 13 754.0 14 819.0 15 901.0 16 992.0 17 1046.5 18 1115.0 19 1182.0 20 1279.5 21 1447.0 22 1721.5 23 2116.5 24 2708.5 25 3382.5 26 4276.0 27 5290.0 28 6140.0 29 7182.5 30 8470.5 31 9776.5 32 10900.5 33 12148.5 34 13914.5 35 15192.0 36 16326.5 37 17743.0 38 18901.0 39 19819.5 40 20746.0 41 21811.5 42 22989.5 43 24122.5 44 24776.5 45 25222.5 46 25211.5 47 25501.0 48 27063.5 49 27248.5 50 25740.5 51 24265.0 52 22549.5 53 21400.5 54 20265.5 55 18355.5 56 16401.5 57 14376.5 58 12579.0 59 11013.0 60 9116.5 61 7287.5 62 5631.0 63 4204.5 64 3166.5 65 2388.5 66 1785.5 67 1363.5 68 1008.5 69 690.5 70 493.0 71 362.5 72 277.0 73 236.0 74 197.0 75 142.5 76 98.5 77 65.0 78 40.5 79 26.5 80 18.0 81 15.0 82 12.5 83 8.0 84 6.5 85 5.5 86 3.0 87 1.5 88 1.0 89 2.0 90 2.5 91 1.5 92 1.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33131117532261234 2 0.3071057926506407 3 0.293187697614257 4 0.27700034795237594 5 0.27352082419328 6 0.22526134249103644 7 0.2167894585558464 8 0.23070755359223008 9 0.23055626995053025 10-11 0.2317665390841288 12-13 0.2269254625497345 14-15 0.22427799881998758 16-17 0.22397543153658794 18-19 0.22405107335743785 20-21 0.2094522019334049 22-23 0.20158545256501414 24-25 0.20211494531096352 26-27 0.21716766766009593 28-29 0.23237167365092812 30-31 0.2250344170284867 32-33 0.231615255442429 34-35 0.2238241478948881 36-37 0.20809064915810654 38-39 0.2071073054870577 40-41 0.20022389978971572 42-43 0.21497405485544846 44-45 0.21225094930485167 46-47 0.21361250208015006 48-49 0.2214792514485409 50-51 0.23191782272582867 52-53 0.2511308452217062 54-55 0.21958820592729308 56-57 0.1868352974992814 58-59 0.18388526648613485 60-61 0.19054174672092705 62-63 0.20150981074416424 64-65 0.18728914842438088 66-67 0.19636616692637024 68-69 0.1980302869850683 70-71 0.18955840304987823 72-73 0.19424819594257273 74-75 0.19833285426846797 76-77 0.21920999682304354 78-79 0.22389978971573804 80-81 0.21194838202145203 82-83 0.2065021709202584 84-85 0.19697130149316952 86-87 0.2032495726237122 88-89 0.2111919638129529 90-91 0.21149453109635252 92-93 0.20688038002450795 94-95 0.20657781274110829 96-97 0.19462640504682227 98-99 0.20430855811561094 100-101 0.1908443140043267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 661010.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.08833976099321 #Duplication Level Percentage of deduplicated Percentage of total 1 84.91472464306854 61.213615207807074 2 9.806726701963367 14.139012928686792 3 2.5259923633473758 5.4628378716797945 4 0.9766521831591549 2.816209376315717 5 0.4836094089809921 1.743129969311744 6 0.28463825016567396 1.2311459332150612 7 0.180590825407368 0.9112944945779186 8 0.1391256413472032 0.8023469202322615 9 0.1016730711889602 0.659649861037207 >10 0.5449923642064256 7.101685745863108 >50 0.028913222906876434 1.4466264027958509 >100 0.011313754406100475 1.2575185995399758 >500 4.1902794080808366E-4 0.23727620938846314 >1k 6.285419112121255E-4 0.9776504795490273 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2896 0.43811742636268736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2157 0.3263188151465182 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1420 0.21482277121374865 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 965 0.1459887142403292 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.02567283399646E-4 0.0 10-11 0.0 0.0 0.0 4.53850925099469E-4 0.0 12-13 0.0 0.0 0.0 4.53850925099469E-4 0.0 14-15 0.0 0.0 0.0 4.53850925099469E-4 0.0 16-17 0.0 0.0 0.0 4.53850925099469E-4 0.0 18-19 0.0 0.0 0.0 4.53850925099469E-4 0.0 20-21 0.0 0.0 0.0 6.05134566799292E-4 0.0 22-23 0.0 0.0 0.0 6.807763876492034E-4 0.0 24-25 0.0 0.0 0.0 9.833436710488495E-4 0.0 26-27 0.0 0.0 0.0 0.001210269133598584 0.0 28-29 0.0 0.0 0.0 0.0021936128046474335 0.0 30-31 0.0 0.0 0.0 0.004311583788444955 7.56418208499115E-5 32-33 0.0 0.0 0.0 0.0055218529220435394 1.51283641699823E-4 34-35 0.0 0.0 0.0 0.007412898443291327 1.51283641699823E-4 36-37 0.0 0.0 0.0 0.008547525756039998 1.51283641699823E-4 38-39 0.0 0.0 0.0 0.010892422202387255 1.51283641699823E-4 40-41 0.0 0.0 0.0 0.015052722349132387 1.51283641699823E-4 42-43 0.0 0.0 0.0 0.02337332264262265 1.51283641699823E-4 44-45 0.0 0.0 0.0 0.03411446120331009 1.51283641699823E-4 46-47 0.0 0.0 0.0 0.047729988956294156 1.51283641699823E-4 48-49 0.0 0.0 0.0 0.07609567177501098 1.51283641699823E-4 50-51 0.0 0.0 0.0 0.10431007095202796 1.51283641699823E-4 52-53 0.0 0.0 0.0 0.13464244111284246 1.51283641699823E-4 54-55 0.0 0.0 0.0 0.19046610490007715 1.51283641699823E-4 56-57 0.0 0.0 0.0 0.24560899229966265 1.51283641699823E-4 58-59 0.0 0.0 0.0 0.30498782166684313 1.51283641699823E-4 60-61 0.0 0.0 0.0 0.3718551912981649 1.51283641699823E-4 62-63 0.0 0.0 0.0 0.4664074673605543 3.02567283399646E-4 64-65 0.0 0.0 0.0 0.5313837914706283 3.02567283399646E-4 66-67 0.0 0.0 0.0 0.6407618644196003 3.02567283399646E-4 68-69 0.0 0.0 0.0 0.7823633530506346 3.02567283399646E-4 70-71 0.0 0.0 0.0 0.8794118092010711 3.02567283399646E-4 72-73 0.0 0.0 0.0 1.035763452897838 3.02567283399646E-4 74-75 0.0 0.0 0.0 1.2328860380327076 3.02567283399646E-4 76-77 0.0 0.0 0.0 1.3803119468691851 3.02567283399646E-4 78-79 0.0 0.0 0.0 1.5340161268362054 3.02567283399646E-4 80-81 0.0 0.0 0.0 1.7483094053040045 3.02567283399646E-4 82-83 0.0 0.0 0.0 1.99996974327166 3.02567283399646E-4 84-85 0.0 0.0 0.0 2.259950681532806 3.02567283399646E-4 86-87 0.0 0.0 0.0 2.6183416287196866 3.02567283399646E-4 88-89 0.0 0.0 0.0 3.0743861666238033 3.02567283399646E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1855 0.0 41.025013 1 GTATCAA 3025 0.0 35.063248 1 ATCAACG 3660 0.0 28.700344 3 TCAACGC 3675 0.0 28.453863 4 TATCAAC 3690 0.0 28.34465 2 CAACGCA 3730 0.0 28.03005 5 AACGCAG 3830 0.0 27.411877 6 AACCGCT 70 0.008384155 27.146086 7 TAGCCCT 495 0.0 25.925941 4 GTGTAGC 500 0.0 25.68422 1 GTACATG 2565 0.0 25.589651 1 TACATGG 2530 0.0 25.180197 2 ACGCAGA 4260 0.0 24.42192 7 ACATGGG 2560 0.0 24.322445 3 CGCAGAG 4280 0.0 24.196802 8 GAGTACT 2490 0.0 23.558464 12-13 GCAGAGT 4510 0.0 22.646818 9 GTACTTT 2655 0.0 22.00326 14-15 AGTACTT 2635 0.0 21.45091 12-13 GTGTATA 395 0.0 20.470312 1 >>END_MODULE