FastQCFastQC Report
Thu 26 May 2016
SRR937753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661010
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30470.4609612562593607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.3862271372596481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.2629309692742924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG250.005021252756.9973649
GGTATCA23800.034.341731
GTATCAA34700.031.0861591
TCAACGC41600.025.579614
ATCAACG42000.025.3359973
GTCCGCG950.001226333324.9988448
CAACGCA42600.024.979155
TATCAAC42950.024.8862022
AACGCAG43900.024.2357856
CGCGGAA550.00287836321.58991282-83
GTACATG26300.021.1397971
ACGCAGA50000.021.0890257
CGGTTAC804.99179E-520.78028928-29
GAGTACT30300.020.77049312-13
CGCAGAG50100.020.667718
TACATGG26400.020.3335762
GGTCCGC1200.0047626719.7907527
GGGCGTA1200.0047626719.7907529
GTACTTT31950.019.62350714-15
AGTACTT31250.019.37910712-13
Produced by FastQC (version 0.11.5)