##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937753_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 661010 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30826159967323 33.0 31.0 34.0 31.0 34.0 2 32.51595134718083 34.0 31.0 34.0 31.0 34.0 3 32.590166563289515 34.0 31.0 34.0 31.0 34.0 4 36.17094597661155 37.0 35.0 37.0 35.0 37.0 5 36.092422202387255 37.0 35.0 37.0 35.0 37.0 6 36.134491157471146 37.0 35.0 37.0 35.0 37.0 7 36.09580641745208 37.0 35.0 37.0 35.0 37.0 8 36.06099605149695 37.0 35.0 37.0 35.0 37.0 9 37.76299450840381 39.0 37.0 39.0 35.0 39.0 10-11 37.689072026141815 39.0 37.0 39.0 35.0 39.0 12-13 37.57854797960697 39.0 37.0 39.0 35.0 39.0 14-15 38.82578402747311 40.0 38.0 41.0 35.0 41.0 16-17 38.79016656328952 40.0 38.0 41.0 35.0 41.0 18-19 38.75185398102903 40.0 38.0 41.0 35.0 41.0 20-21 38.64686237727115 40.0 38.0 41.0 34.0 41.0 22-23 38.5937875372536 40.0 38.0 41.0 34.0 41.0 24-25 38.60424123689505 40.0 38.0 41.0 34.0 41.0 26-27 38.4264769065521 40.0 38.0 41.0 34.0 41.0 28-29 38.401013600399395 40.0 38.0 41.0 34.0 41.0 30-31 38.42882785434411 40.0 38.0 41.0 34.0 41.0 32-33 38.30770487587178 40.0 38.0 41.0 34.0 41.0 34-35 38.15634256667826 40.0 38.0 41.0 34.0 41.0 36-37 37.99488358723771 40.0 37.5 41.0 33.5 41.0 38-39 37.85151586208983 40.0 37.0 41.0 33.0 41.0 40-41 37.66066700957626 40.0 37.0 41.0 33.0 41.0 42-43 37.43862422656238 40.0 37.0 41.0 33.0 41.0 44-45 37.122389222553366 40.0 37.0 41.0 31.5 41.0 46-47 36.76905114899925 40.0 36.0 41.0 31.0 41.0 48-49 36.42589068244051 39.5 36.0 41.0 30.0 41.0 50-51 36.183460915871166 39.0 36.0 41.0 30.0 41.0 52-53 35.92161086821682 39.0 35.0 40.5 29.0 41.0 54-55 35.67322430825555 39.0 35.0 40.0 28.0 41.0 56-57 35.407844056822135 39.0 35.0 40.0 28.0 41.0 58-59 35.152081662909794 38.0 35.0 40.0 27.0 41.0 60-61 34.809955220042056 38.0 34.0 40.0 26.5 41.0 62-63 34.40130179573683 37.5 34.0 40.0 26.0 41.0 64-65 34.02205942421446 37.0 34.0 40.0 25.5 41.0 66-67 33.59728975355895 36.5 33.0 39.0 24.5 40.5 68-69 33.12151555952255 36.0 32.5 39.0 22.5 40.0 70-71 32.6837400341901 35.5 32.0 38.5 22.0 40.0 72-73 32.20589627993525 35.0 31.5 38.0 20.5 40.0 74-75 31.709920424804466 35.0 31.0 37.0 19.5 39.0 76-77 30.459270661563366 33.5 29.5 36.0 18.5 38.5 78-79 31.06260570944464 34.0 31.0 36.0 18.5 38.5 80-81 30.897486422293156 34.0 31.0 36.0 18.0 37.5 82-83 30.499669445242887 34.0 31.0 35.5 15.0 37.0 84-85 30.080075187969925 34.0 30.0 35.0 9.0 36.5 86-87 29.721467148757206 34.0 30.0 35.0 7.0 36.0 88-89 29.31419494410069 34.0 29.5 35.0 2.0 36.0 90-91 28.818126805948474 34.0 29.0 35.0 2.0 35.5 92-93 28.361715405213232 34.0 29.0 35.0 2.0 35.0 94-95 27.868542079544937 33.0 27.5 35.0 2.0 35.0 96-97 27.358176880833874 33.0 27.0 35.0 2.0 35.0 98-99 26.552291947171753 33.0 25.5 34.5 2.0 35.0 100-101 24.111821303762426 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10646655232019953 1101 2 0.1155900833577519 1101 3 0.08675935022000658 1101 4 0.0931335814386216 1101 5 -0.0164673124737007 1101 6 -0.0222746432975498 1101 7 0.006977567738417179 1101 8 0.09455254381171585 1101 9 0.09021785492189593 1101 10-11 0.12475812444186118 1101 12-13 0.04042424543762735 1101 14-15 0.007170483265333871 1101 16-17 0.0942726518196011 1101 18-19 0.18205778301049236 1101 20-21 0.16600318317756546 1101 22-23 0.016212625373462686 1101 24-25 0.0393878472868181 1101 26-27 0.02318852769474944 1101 28-29 -0.005399970518688235 1101 30-31 0.11759857281044361 1101 32-33 0.005758324465183762 1101 34-35 0.03519893556962472 1101 36-37 -0.028160938215989972 1101 38-39 -0.002029425978022914 1101 40-41 -0.1760386119267281 1101 42-43 -0.1515268053001506 1101 44-45 -0.05960497916603913 1101 46-47 -0.025480451742168952 1101 48-49 -0.11776341742432095 1101 50-51 0.03182716723719636 1101 52-53 -0.027349762146748446 1101 54-55 -0.0391528197013713 1101 56-57 0.07880320638048488 1101 58-59 0.24464859883001822 1101 60-61 0.14462609910700053 1101 62-63 0.08040756608244948 1101 64-65 0.1546720590885755 1101 66-67 0.09899650726354992 1101 68-69 0.14824586082571045 1101 70-71 -0.12359925771760771 1101 72-73 0.02317037463049587 1101 74-75 0.17156264650115816 1101 76-77 0.0605085715794722 1101 78-79 0.3321578370603646 1101 80-81 0.2854715623376798 1101 82-83 0.0955297742803154 1101 84-85 0.1766352855549833 1101 86-87 0.1265664625566707 1101 88-89 0.2947292738358023 1101 90-91 0.07831971856377962 1101 92-93 0.18839440256633466 1101 94-95 0.46805663355491944 1101 96-97 0.4919617307158397 1101 98-99 0.3260963563339594 1101 100-101 0.5602212428594875 1102 1 0.12723652947882869 1102 2 0.14321789558843534 1102 3 0.19987114034458386 1102 4 0.10771408973443641 1102 5 -0.006138673976046505 1102 6 0.058019100336700546 1102 7 0.015033520054799965 1102 8 0.0159264009898763 1102 9 0.12862450362886335 1102 10-11 0.1352479784137941 1102 12-13 0.12638463765829755 1102 14-15 0.10440059048116268 1102 16-17 0.21564108492572132 1102 18-19 0.1851681869833186 1102 20-21 0.20649086752067802 1102 22-23 0.18796978364186145 1102 24-25 0.19091412088906878 1102 26-27 0.2015485896496969 1102 28-29 0.3297648911373443 1102 30-31 0.31876896701994895 1102 32-33 0.19971319618587557 1102 34-35 0.21031359442562092 1102 36-37 0.3517296160639063 1102 38-39 0.24209148261431324 1102 40-41 0.3425765019486491 1102 42-43 0.4597046398534488 1102 44-45 0.5318692836121741 1102 46-47 0.5630485399228462 1102 48-49 0.43832781546725386 1102 50-51 0.28668894380159315 1102 52-53 0.4847293449304573 1102 54-55 0.44060333812707597 1102 56-57 0.3606199089062869 1102 58-59 0.5536667212573505 1102 60-61 0.5709898012169674 1102 62-63 0.6076312849467698 1102 64-65 0.6420776651683653 1102 66-67 0.6128004692468352 1102 68-69 0.34773629939206785 1102 70-71 0.42447399136180763 1102 72-73 0.5530428486702874 1102 74-75 0.2996738038173312 1102 76-77 0.2282386577016844 1102 78-79 0.2626950612613719 1102 80-81 0.3797389208748676 1102 82-83 0.3695924644995081 1102 84-85 0.3438317032567433 1102 86-87 0.45822677100556675 1102 88-89 0.5879323089054438 1102 90-91 0.5060331105976239 1102 92-93 0.663473503616796 1102 94-95 0.8065792971358938 1102 96-97 0.8249967400874283 1102 98-99 0.7259875735559902 1102 100-101 1.2035414981524788 1103 1 0.03451400053433673 1103 2 0.03791820075767305 1103 3 0.08562369701054706 1103 4 0.020955173434671792 1103 5 -0.033645837299829395 1103 6 -0.022347568659505157 1103 7 0.013875740568693118 1103 8 -0.05074026567679368 1103 9 -0.011163974485143058 1103 10-11 0.11322104897543994 1103 12-13 0.0711711356665532 1103 14-15 0.071400380822908 1103 16-17 0.10896463420706226 1103 18-19 0.027537791195122452 1103 20-21 0.09305410278054183 1103 22-23 0.12454466658266483 1103 24-25 -0.06881285746954546 1103 26-27 -0.17965088853419076 1103 28-29 0.03562833372592422 1103 30-31 -0.18341147885328013 1103 32-33 -0.2680774716921448 1103 34-35 -0.07568938738069875 1103 36-37 -0.027148479307307127 1103 38-39 -0.004123021078001443 1103 40-41 -0.05635074663615569 1103 42-43 -0.05086679537379979 1103 44-45 -0.097339605897929 1103 46-47 -0.16090957501647551 1103 48-49 -0.2692233760905083 1103 50-51 0.026108190429582123 1103 52-53 -0.04241557551587505 1103 54-55 -0.06499919640830143 1103 56-57 0.012424134684934529 1103 58-59 -0.04679336214004337 1103 60-61 -0.10472128970483396 1103 62-63 -0.39358356817597695 1103 64-65 -0.14460088213287037 1103 66-67 -0.04624744931258107 1103 68-69 0.10104191124474937 1103 70-71 -0.10433421792372144 1103 72-73 -0.24291657080929951 1103 74-75 -0.27618987172487053 1103 76-77 -0.1573566552715029 1103 78-79 -0.18040019347341385 1103 80-81 -0.12790195782618596 1103 82-83 0.038113441972893014 1103 84-85 0.02948641940275465 1103 86-87 0.17164955852857133 1103 88-89 0.11394623953185246 1103 90-91 0.04473369531120852 1103 92-93 0.3364194350818437 1103 94-95 0.4567167397710641 1103 96-97 0.43868975380839714 1103 98-99 0.7252246504539812 1103 100-101 1.404797118158534 1104 1 0.1738924583022694 1104 2 0.1840466269889589 1104 3 0.2638034919322507 1104 4 0.09999051249866397 1104 5 0.16422296124407865 1104 6 0.10382942400597273 1104 7 0.05548834441436412 1104 8 0.16624086639868096 1104 9 0.1737196102403047 1104 10-11 0.2243663247859402 1104 12-13 0.21298917823200725 1104 14-15 0.08063160750830889 1104 16-17 0.1401876027965656 1104 18-19 0.05809060606600269 1104 20-21 0.24244826129272923 1104 22-23 0.24255254173359475 1104 24-25 0.15942347815244773 1104 26-27 0.13296061603411857 1104 28-29 0.17048240916157908 1104 30-31 0.18199856511781576 1104 32-33 0.23740877833037644 1104 34-35 0.29426531248054033 1104 36-37 0.16816810356198886 1104 38-39 0.36549998085023816 1104 40-41 0.37700950547679923 1104 42-43 0.5256934801663817 1104 44-45 0.4783804432992369 1104 46-47 0.3937434317774233 1104 48-49 0.6258508251313089 1104 50-51 0.7045070524072088 1104 52-53 0.5814034897379514 1104 54-55 0.5243345469014287 1104 56-57 0.5247650998865083 1104 58-59 0.4031653023280697 1104 60-61 0.391629469877806 1104 62-63 0.33281632099524217 1104 64-65 0.42527869293335385 1104 66-67 0.48622910093881444 1104 68-69 0.44569802665085234 1104 70-71 0.4936630924509302 1104 72-73 0.637322454438042 1104 74-75 0.5561050049217933 1104 76-77 0.22374793826618955 1104 78-79 0.3676239230499867 1104 80-81 0.4927499271641729 1104 82-83 0.4815507786581179 1104 84-85 0.4469792335527991 1104 86-87 0.6642035312448691 1104 88-89 0.4088634333110299 1104 90-91 0.5034358947669766 1104 92-93 0.6933342951492456 1104 94-95 0.5471683445358018 1104 96-97 0.7837423299018162 1104 98-99 0.6724373741389016 1104 100-101 1.2250900485436418 1105 1 -0.014528423381968025 1105 2 0.008350152544807088 1105 3 -0.12590846956419455 1105 4 0.009614925644200412 1105 5 0.028916099106893967 1105 6 -0.02054496699411601 1105 7 -0.06805743761534444 1105 8 -0.006921799323116318 1105 9 0.030914667375569138 1105 10-11 -0.05418100742536325 1105 12-13 0.0667812176804361 1105 14-15 -0.03788346616222782 1105 16-17 -0.026681141113435558 1105 18-19 -0.1422565238835034 1105 20-21 -0.24780128103461152 1105 22-23 -0.14715941976285052 1105 24-25 -0.23670143695665757 1105 26-27 -0.14368405281756935 1105 28-29 -0.02825640738550561 1105 30-31 -0.26124172041087235 1105 32-33 -0.26316515794977846 1105 34-35 -0.2049789739930148 1105 36-37 -0.22270833958325653 1105 38-39 -0.24043647646809063 1105 40-41 -0.11216413700703498 1105 42-43 0.19148447877859098 1105 44-45 -0.1755756570344218 1105 46-47 -0.10455136847060942 1105 48-49 -0.060807647718363 1105 50-51 -0.25822307574114234 1105 52-53 0.038748582305927926 1105 54-55 0.21782765966606377 1105 56-57 0.12591629426451334 1105 58-59 -0.04000670139236462 1105 60-61 -0.04131410351222087 1105 62-63 -0.15606389425854417 1105 64-65 -0.07496306157693056 1105 66-67 0.030138917279202815 1105 68-69 0.12085931935466476 1105 70-71 -0.13459505707515973 1105 72-73 -0.18962099346109085 1105 74-75 0.05324455079240309 1105 76-77 0.15477593011666357 1105 78-79 -0.19815268101887185 1105 80-81 0.0761395468529571 1105 82-83 0.03964475657172528 1105 84-85 0.048350959716909614 1105 86-87 0.25065014594354196 1105 88-89 0.4259375762820632 1105 90-91 0.5109035254989571 1105 92-93 0.486228188910129 1105 94-95 0.6071308449048907 1105 96-97 0.7116718040211651 1105 98-99 0.5491010511696395 1105 100-101 1.1428750605021882 1106 1 -0.4017442907161133 1106 2 -0.4174792251949242 1106 3 -0.2890432018375151 1106 4 -0.2807645519580575 1106 5 -0.1335788213056972 1106 6 -0.2741957853480699 1106 7 -0.19438569568431774 1106 8 -0.20543639827347704 1106 9 -0.22807944462608987 1106 10-11 -0.4943369831816824 1106 12-13 -0.4164150748692137 1106 14-15 -0.4116978688459767 1106 16-17 -0.3367867100704629 1106 18-19 -0.3439788282437348 1106 20-21 -0.6445624496423292 1106 22-23 -0.7900363251485132 1106 24-25 -0.5554228507679468 1106 26-27 -0.4958529285643749 1106 28-29 -0.5711065098418615 1106 30-31 -0.6796095084775189 1106 32-33 -0.7085044743886186 1106 34-35 -0.8843165093339778 1106 36-37 -0.7096008425445461 1106 38-39 -0.8223540099069098 1106 40-41 -0.9037627155871704 1106 42-43 -0.6937427841235575 1106 44-45 -0.7440571413979384 1106 46-47 -0.7006917466512945 1106 48-49 -0.5499913688295592 1106 50-51 -1.0409421032271098 1106 52-53 -1.3726461210893888 1106 54-55 -1.0417889719838698 1106 56-57 -0.9746897977819344 1106 58-59 -1.111946628246308 1106 60-61 -1.6329023067648833 1106 62-63 -1.7827855501608028 1106 64-65 -2.25006400486712 1106 66-67 -2.210473572885853 1106 68-69 -2.2048913788184166 1106 70-71 -1.9489415411360582 1106 72-73 -2.1114385779119367 1106 74-75 -1.8579599382659495 1106 76-77 -1.6348772745626086 1106 78-79 -1.7291187104675068 1106 80-81 -2.018051134373753 1106 82-83 -1.9692477636817785 1106 84-85 -1.8874084710234555 1106 86-87 -1.9811389928178151 1106 88-89 -2.031692180157126 1106 90-91 -2.1980858311442795 1106 92-93 -1.8019422085513703 1106 94-95 -2.4524581192051187 1106 96-97 -2.8292502167467823 1106 98-99 -2.83185503525684 1106 100-101 -1.9294280028132498 1107 1 -0.08122426699354435 1107 2 -0.11430242961481696 1107 3 -0.07605899607542455 1107 4 -0.10339931800981361 1107 5 -0.17026296776136007 1107 6 -0.07435653697899625 1107 7 -0.27953723921211804 1107 8 -0.1545538249988283 1107 9 -0.22466542333820883 1107 10-11 -0.08149792209605522 1107 12-13 -0.16694766219011825 1107 14-15 -0.1803813442025941 1107 16-17 -0.07870230766074116 1107 18-19 0.07271124885461688 1107 20-21 -0.18882572335709114 1107 22-23 -0.13438621746052348 1107 24-25 -0.21235858132948948 1107 26-27 -0.39016458383796504 1107 28-29 -0.32402110858706124 1107 30-31 -0.31045626379168567 1107 32-33 -0.27926055329738375 1107 34-35 -0.35938635045518197 1107 36-37 -0.636536885245178 1107 38-39 -0.6092761624180696 1107 40-41 -0.3710017673086199 1107 42-43 -0.7353294651486237 1107 44-45 -0.8103847469789827 1107 46-47 -0.790718512578195 1107 48-49 -0.4194801630362619 1107 50-51 -0.6147786047721979 1107 52-53 -0.3822697593826163 1107 54-55 -0.3919290444001433 1107 56-57 -0.30856157104410187 1107 58-59 -0.19586132063323447 1107 60-61 -0.2533177638925821 1107 62-63 -0.6450678331461077 1107 64-65 -0.4549579556115688 1107 66-67 -0.4159235213829007 1107 68-69 -0.11182515851064778 1107 70-71 -0.06472953626668954 1107 72-73 -0.16335511191948626 1107 74-75 -0.16475364554191074 1107 76-77 -0.3412304755489721 1107 78-79 -0.174219297288996 1107 80-81 0.013566604490684142 1107 82-83 -0.04026525907772793 1107 84-85 0.05222187826018754 1107 86-87 0.12147088014157603 1107 88-89 0.01175758105646807 1107 90-91 0.08627287909700598 1107 92-93 0.3523325362749219 1107 94-95 0.5112713186323816 1107 96-97 0.28512144726670385 1107 98-99 0.1570971918555628 1107 100-101 0.7228314940185392 1108 1 0.18607799289345905 1108 2 0.2167160889037021 1108 3 -0.10132578829136918 1108 4 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1.8920685454341246 2316 1 0.06691132622679419 2316 2 0.011874040973417266 2316 3 0.024175301033245944 2316 4 0.03351992426336636 2316 5 -0.19751122299565793 2316 6 -0.003798211954070041 2316 7 -0.08603773771660173 2316 8 -0.10485791273561773 2316 9 0.05861972122809789 2316 10-11 0.06103946574042851 2316 12-13 -0.048914262389523344 2316 14-15 -0.04739280980578542 2316 16-17 0.22802750290755114 2316 18-19 0.2379754562324834 2316 20-21 0.20213888282436443 2316 22-23 0.07821846848165137 2316 24-25 0.33873812854090346 2316 26-27 -0.11196169251194732 2316 28-29 -0.2155913977196633 2316 30-31 -0.35416264464911507 2316 32-33 -0.17915337483277227 2316 34-35 -0.052120647563853595 2316 36-37 -0.1416515412770849 2316 38-39 -0.31386738873911213 2316 40-41 -0.33079987299156954 2316 42-43 0.028685988490465775 2316 44-45 -0.11652382973072406 2316 46-47 -0.07951960928806301 2316 48-49 -0.27534506832040506 2316 50-51 0.006617207790114321 2316 52-53 -0.08144473445739209 2316 54-55 0.03630874223993885 2316 56-57 0.03918518867673981 2316 58-59 -0.2774714901247606 2316 60-61 -0.2571163680800268 2316 62-63 -0.20955188100253253 2316 64-65 -0.504058155013368 2316 66-67 -0.42166629306784387 2316 68-69 -0.03455447057445582 2316 70-71 0.2208537139825637 2316 72-73 0.20768177741251748 2316 74-75 -0.0045442736575651566 2316 76-77 0.1609454965347794 2316 78-79 0.06710921716763352 2316 80-81 0.08052323460035282 2316 82-83 0.07017591739285933 2316 84-85 0.49317651435191223 2316 86-87 0.5641952071605196 2316 88-89 0.6738911802588596 2316 90-91 0.3547275151887277 2316 92-93 0.8352238622968571 2316 94-95 0.7340828839364697 2316 96-97 0.8254654153624195 2316 98-99 0.34150646403901064 2316 100-101 0.822709360419335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 18.0 6 38.0 7 56.0 8 154.0 9 311.0 10 527.0 11 907.0 12 1395.0 13 2062.0 14 2667.0 15 3679.0 16 4948.0 17 7118.0 18 6623.0 19 4201.0 20 3524.0 21 3463.0 22 3558.0 23 3813.0 24 4367.0 25 5195.0 26 6118.0 27 7574.0 28 9193.0 29 11449.0 30 14122.0 31 17991.0 32 22930.0 33 30178.0 34 41831.0 35 61319.0 36 96360.0 37 150452.0 38 122343.0 39 10518.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.50256903865741 19.97236486251292 15.43087961764835 22.094186481181318 2 15.347725450447044 20.759443881333112 40.56701108909094 23.325819579128908 3 19.316803074083598 24.73321129786236 30.2567283399646 25.693257288089438 4 12.849729958699566 17.389449478827853 37.53801001497708 32.2228105474955 5 13.677069322629599 37.67756557576711 34.25614226730191 14.38922283430138 6 31.524333973767416 37.04316122297696 17.261160950666405 14.171343852589219 7 28.319238740714965 33.27181131904207 20.52026444380569 17.88868549643727 8 26.167531504818385 35.90354155005219 19.42693756524107 18.501989379888354 9 26.187803512806163 17.388995627902755 20.5986293702062 35.82457148908488 10-11 24.83789957791864 27.48732999500764 27.08552064265291 20.58924978442081 12-13 25.78499692870046 24.56842946147864 28.084991784651976 21.561581825168922 14-15 22.53785873133538 26.059363701003015 26.71578342233854 24.686994145323066 16-17 21.75156200360055 29.466120028441324 27.156548312430978 21.625769655527147 18-19 21.539840547041646 27.570687281584245 30.063160920409675 20.826311250964434 20-21 22.463578463260767 26.649445545453172 30.282143991770173 20.604831999515895 22-23 21.968296424497503 26.717856937539903 30.172557254333597 21.141289383628997 24-25 21.97334382233249 26.811394683892832 30.259980938261144 20.955280555513532 26-27 21.857803213735416 27.244588366377638 30.036467114922473 20.861141304964477 28-29 21.56419416988087 27.244161673589275 30.202413469924664 20.98923068660519 30-31 21.715556496875994 26.975461793316292 30.18063266818959 21.12834904161813 32-33 21.63318255397044 27.02644438056913 30.140239935855735 21.200133129604694 34-35 22.08786553909926 27.011316016399146 29.911196502322202 20.989621942179394 36-37 22.03699193203118 26.88390021921033 29.915628985384462 21.163478863374024 38-39 21.952012828852816 27.165020196366168 29.899472020090467 20.98349495469055 40-41 22.054590908575168 27.003778323241416 29.83687779639414 21.104752971789274 42-43 21.973913879029947 27.12304390031316 29.627169272277577 21.27587294837932 44-45 22.04285865569356 27.481581216623045 29.260298633908715 21.21526149377468 46-47 21.99043887384457 27.718340115883272 28.7593984962406 21.531822514031557 48-49 22.377271145671017 27.746251947776884 28.56106564197213 21.31541126457996 50-51 22.3913405243491 27.87295199770049 28.21197863874979 21.52372883920062 52-53 22.38491096957686 28.04450764738809 28.078773392233096 21.491807990801952 54-55 22.187485817158592 28.054794935023676 28.261826598689883 21.49589264912785 56-57 22.29255230631912 28.35320191827658 28.04715511111783 21.30709066428647 58-59 22.35646964493729 27.994281478343748 28.224913390115127 21.424335486603834 60-61 22.492095429721186 27.663878004871336 28.569840093190724 21.27418647221676 62-63 22.28287015325033 27.87121223582094 28.49404698869911 21.35187062222962 64-65 22.24784799019682 27.734376181903453 28.457058138303502 21.560717689596224 66-67 22.27500340388194 27.95797340433579 28.181873194051526 21.585149997730745 68-69 22.242250495453927 27.95532594060604 28.17188847369934 21.630535090240695 70-71 22.331507844056823 28.024840773967114 28.074007957519555 21.569643424456515 72-73 22.393987988078848 27.822650186835297 27.965159377316528 21.818202447769323 74-75 22.32817960393943 28.03338829972315 27.694739867778097 21.943692228559325 76-77 22.15344699777613 28.022798444804163 28.008199573380132 21.815554984039576 78-79 22.346560566405955 27.88097003071058 27.917353746539387 21.85511565634408 80-81 22.073493593137776 28.274836991876068 27.869548115762242 21.782121299223913 82-83 22.236594179054915 28.195736975035913 27.68522993996304 21.882438905946135 84-85 22.314446550652107 28.020344654913927 27.616341078563227 22.04886771587074 86-87 22.259875039711957 27.91228574454244 27.749428904252582 22.07841031149302 88-89 22.314715359828142 27.76516240298936 27.813573168333306 22.106549068849183 90-91 22.303461736314944 27.791857052077347 27.9596483795806 21.94503283202711 92-93 22.225979187421377 27.77837031926892 27.918764830872266 22.076885662437434 94-95 22.15420341598463 27.84352732938987 27.761758521051117 22.24051073357438 96-97 22.226668280358844 27.87968411975613 27.758505922754573 22.135141677130452 98-99 22.21053125245838 27.84158643518042 27.553691051793937 22.394191260567258 100-101 22.246186969918654 27.864907413781754 27.58144084536131 22.307464770938285 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 148.0 1 94.5 2 64.5 3 86.5 4 74.0 5 71.5 6 147.0 7 323.5 8 529.5 9 673.5 10 669.5 11 661.5 12 739.0 13 829.5 14 936.5 15 1029.5 16 1081.0 17 1128.0 18 1211.0 19 1340.5 20 1489.5 21 1631.5 22 1863.0 23 2281.5 24 2861.5 25 3423.0 26 4110.5 27 5215.0 28 6335.0 29 7432.5 30 8586.0 31 9760.0 32 10892.5 33 12300.5 34 14076.0 35 15307.0 36 16593.5 37 17976.0 38 19100.0 39 20072.5 40 20918.5 41 21870.5 42 23181.5 43 24361.0 44 25014.0 45 25494.5 46 25303.5 47 25324.0 48 26902.5 49 27175.0 50 25719.0 51 24402.0 52 22699.5 53 20969.5 54 19613.5 55 18040.5 56 16001.5 57 14004.0 58 12443.0 59 11050.0 60 9189.0 61 7159.5 62 5453.0 63 4097.0 64 2985.5 65 2259.0 66 1695.5 67 1305.0 68 942.5 69 581.0 70 426.5 71 349.0 72 291.0 73 215.0 74 159.5 75 129.5 76 81.0 77 46.0 78 27.5 79 22.5 80 16.0 81 8.5 82 6.0 83 2.5 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03887989591685451 2 0.0 3 0.0 4 0.0 5 0.008623167576889912 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007866749368390796 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001815403700397876 24-25 0.0 26-27 5.294927459493805E-4 28-29 0.0017397618795479647 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.51283641699823E-4 38-39 0.0 40-41 3.782091042495575E-4 42-43 0.02428102449282159 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.56418208499115E-5 84-85 1.51283641699823E-4 86-87 0.0 88-89 0.0 90-91 0.01058985491898761 92-93 0.0023448964463472564 94-95 0.0 96-97 0.0 98-99 9.07701850198938E-4 100-101 0.012934751365334868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 661010.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.06525995259013 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80238750963863 58.716360655105234 2 10.339451802163628 14.488727565717424 3 2.714780020097498 5.706353034666874 4 1.1331257877378842 3.175710115073533 5 0.5661838618594355 1.9834909731071362 6 0.3312392855936058 1.392501999097574 7 0.20570848988424417 1.0089113172736028 8 0.16280981430270933 0.9125849569561804 9 0.10671086585980676 0.6729052100610002 >10 0.5864113851040649 7.392177026275386 >50 0.03671951392320599 1.7299525022326554 >100 0.013607683905006112 1.6112897880730885 >500 2.1599498258585522E-4 0.09852067736769864 >1k 6.479849477575657E-4 1.1105141789925845 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3047 0.4609612562593607 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2553 0.3862271372596481 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1738 0.2629309692742924 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.51283641699823E-4 0.0 3 0.0 0.0 0.0 1.51283641699823E-4 0.0 4 0.0 0.0 0.0 1.51283641699823E-4 0.0 5 0.0 0.0 0.0 1.51283641699823E-4 0.0 6 0.0 0.0 0.0 1.51283641699823E-4 0.0 7 0.0 0.0 0.0 1.51283641699823E-4 0.0 8 0.0 0.0 0.0 1.51283641699823E-4 0.0 9 0.0 0.0 0.0 3.02567283399646E-4 0.0 10-11 0.0 0.0 0.0 3.02567283399646E-4 0.0 12-13 0.0 0.0 0.0 3.02567283399646E-4 0.0 14-15 0.0 0.0 0.0 3.02567283399646E-4 0.0 16-17 0.0 0.0 0.0 3.02567283399646E-4 0.0 18-19 0.0 0.0 0.0 3.02567283399646E-4 0.0 20-21 0.0 0.0 0.0 4.53850925099469E-4 0.0 22-23 0.0 0.0 0.0 4.53850925099469E-4 0.0 24-25 0.0 0.0 0.0 8.320600293490265E-4 0.0 26-27 0.0 0.0 0.0 0.001058985491898761 0.0 28-29 0.0 0.0 0.0 0.0018910455212477876 0.0 30-31 0.0 0.0 0.0 0.003933374684195398 0.0 32-33 0.0 0.0 0.0 0.005219285638643894 0.0 34-35 0.0 0.0 0.0 0.007110331159891681 0.0 36-37 0.0 0.0 0.0 0.008018033010090618 0.0 38-39 0.0 0.0 0.0 0.010665496739837521 0.0 40-41 0.0 0.0 0.0 0.014977080528282477 0.0 42-43 0.0 0.0 0.0 0.0236758899260223 0.0 44-45 0.0 0.0 0.0 0.03471959577010938 0.0 46-47 0.0 0.0 0.0 0.04848640716479327 0.0 48-49 0.0 0.0 0.0 0.07670080634181026 0.0 50-51 0.0 0.0 0.0 0.10491520551882724 0.0 52-53 0.0 0.0 0.0 0.1353988593213416 0.0 54-55 0.0 0.0 0.0 0.19061738854177696 0.0 56-57 0.0 0.0 0.0 0.24545770865796282 0.0 58-59 0.0 0.0 0.0 0.3060468071587419 0.0 60-61 0.0 0.0 0.0 0.3738218786402626 0.0 62-63 0.0 0.0 0.0 0.46882800562775145 0.0 64-65 0.0 0.0 0.0 0.5339556133795252 0.0 66-67 0.0 0.0 0.0 0.6445439554620959 0.0 68-69 0.0 0.0 0.0 0.7861454440931301 0.0 70-71 0.0 0.0 0.0 0.8844798111980151 0.0 72-73 0.0 0.0 0.0 1.0426468585951802 0.0 74-75 0.0 0.0 0.0 1.2412066383261977 0.0 76-77 0.0 0.0 0.0 1.389918458117124 0.0 78-79 0.0 0.0 0.0 1.5443790562926432 0.0 80-81 0.0 0.0 0.0 1.7616223657735888 0.0 82-83 0.0 0.0 0.0 2.0157032420084415 0.0 84-85 0.0 0.0 0.0 2.2774239421491353 0.0 86-87 0.0 0.0 0.0 2.643303429600157 0.0 88-89 0.0 0.0 0.0 3.104340327680368 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGTG 25 0.0050212527 56.997364 9 GGTATCA 2380 0.0 34.34173 1 GTATCAA 3470 0.0 31.086159 1 TCAACGC 4160 0.0 25.57961 4 ATCAACG 4200 0.0 25.335997 3 GTCCGCG 95 0.0012263333 24.998844 8 CAACGCA 4260 0.0 24.97915 5 TATCAAC 4295 0.0 24.886202 2 AACGCAG 4390 0.0 24.235785 6 CGCGGAA 55 0.002878363 21.589912 82-83 GTACATG 2630 0.0 21.139797 1 ACGCAGA 5000 0.0 21.089025 7 CGGTTAC 80 4.99179E-5 20.780289 28-29 GAGTACT 3030 0.0 20.770493 12-13 CGCAGAG 5010 0.0 20.66771 8 TACATGG 2640 0.0 20.333576 2 GGTCCGC 120 0.00476267 19.790752 7 GGGCGTA 120 0.00476267 19.790752 9 GTACTTT 3195 0.0 19.623507 14-15 AGTACTT 3125 0.0 19.379107 12-13 >>END_MODULE