FastQCFastQC Report
Thu 26 May 2016
SRR937752_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937752_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437409
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19980.45678072467644704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.34910118447494226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.22496107761843034No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6860.15683262118520652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11400.043.3975561
GTATCAA18800.038.967241
TATCAAC23700.030.6923962
TCAACGC23600.030.6209934
ATCAACG23600.030.6174813
CAACGCA23750.030.223955
AACGCAG24550.029.4158276
CCTACGT1153.830184E-628.939292
GTATAGC1754.8657967E-927.1830831
CACGGAC700.00835359727.1643813
ACGCAGA27950.025.4975477
CGCAGAG28350.025.1377948
ACGTCTA950.001219684625.0198275
CGTCTAT1000.001649897723.7552156
GTACATG16150.023.269731
GCAGAGT29400.023.2677489
GAGTACT16250.022.50489612-13
TACATGG16100.022.4427132
ACATGGG15950.021.7570513
CATGGGG7850.021.1975674
Produced by FastQC (version 0.11.5)