##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937752_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 437409 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80729934683557 33.0 31.0 34.0 30.0 34.0 2 31.923657263567964 33.0 31.0 34.0 30.0 34.0 3 31.9702863909979 34.0 31.0 34.0 30.0 34.0 4 35.55986730954324 37.0 35.0 37.0 33.0 37.0 5 35.55600593494876 37.0 35.0 37.0 33.0 37.0 6 35.61032351872046 37.0 35.0 37.0 33.0 37.0 7 35.54837691954212 37.0 35.0 37.0 33.0 37.0 8 35.54979207103649 37.0 35.0 37.0 33.0 37.0 9 37.12100345443281 39.0 37.0 39.0 34.0 39.0 10-11 37.063143419545554 39.0 37.0 39.0 33.0 39.0 12-13 36.970461284518606 39.0 37.0 39.0 33.0 39.0 14-15 38.13020308224111 40.0 38.0 41.0 33.0 41.0 16-17 38.04738699935301 40.0 38.0 41.0 33.0 41.0 18-19 37.98165904222364 40.0 38.0 41.0 33.0 41.0 20-21 37.98830385291569 40.0 38.0 41.0 33.0 41.0 22-23 37.994415981381266 40.0 38.0 41.0 33.0 41.0 24-25 37.915795056800384 40.0 38.0 41.0 33.0 41.0 26-27 37.81552048540382 40.0 38.0 41.0 33.0 41.0 28-29 37.659563474917064 40.0 37.5 41.0 33.0 41.0 30-31 37.426256661385565 40.0 37.0 41.0 32.0 41.0 32-33 37.29124915125203 40.0 37.0 41.0 32.0 41.0 34-35 37.281275648191965 40.0 37.0 41.0 32.0 41.0 36-37 37.12106632465267 40.0 37.0 41.0 31.0 41.0 38-39 36.956641267097844 40.0 36.0 41.0 31.0 41.0 40-41 36.72735471835284 39.5 36.0 41.0 30.5 41.0 42-43 36.470190371025744 39.0 36.0 41.0 30.0 41.0 44-45 36.20171509959786 39.0 35.5 41.0 30.0 41.0 46-47 35.81428022743016 39.0 35.0 41.0 28.5 41.0 48-49 35.45586739184607 39.0 35.0 40.0 27.0 41.0 50-51 34.57906444540464 38.0 34.0 39.5 25.0 40.5 52-53 34.68222075906074 38.0 34.5 39.5 26.0 40.5 54-55 34.958502225605784 39.0 35.0 40.0 26.0 41.0 56-57 34.73459622458614 38.0 34.5 40.0 24.5 41.0 58-59 34.46307346213727 38.0 34.0 40.0 23.5 41.0 60-61 34.133430039162434 38.0 34.0 40.0 22.5 41.0 62-63 33.778068123884054 37.5 33.5 40.0 22.0 41.0 64-65 33.33737874620778 37.0 33.0 40.0 20.5 41.0 66-67 32.82779846779559 36.5 32.0 39.0 18.0 41.0 68-69 32.370835991029 36.0 31.5 39.0 16.5 40.0 70-71 31.90920168537913 35.0 31.0 38.5 12.0 40.0 72-73 31.42997171983201 35.0 31.0 38.0 8.0 40.0 74-75 30.887804091822527 35.0 30.5 37.0 7.0 39.0 76-77 30.331862170188543 34.5 30.0 36.5 2.0 39.0 78-79 29.80823668465898 34.0 29.0 36.0 2.0 39.0 80-81 29.26209908803888 34.0 29.0 36.0 2.0 37.5 82-83 28.720880228801875 34.0 28.5 35.0 2.0 37.0 84-85 28.177673527522295 34.0 27.0 35.0 2.0 36.5 86-87 27.588477831960475 33.0 26.0 35.0 2.0 36.0 88-89 27.053220212661376 33.0 25.5 35.0 2.0 36.0 90-91 26.48223973443619 32.5 25.0 35.0 2.0 35.0 92-93 25.822661399285337 32.0 23.5 34.0 2.0 35.0 94-95 25.064560857229733 31.5 19.0 34.0 2.0 35.0 96-97 24.187362399950615 31.0 9.5 34.0 2.0 35.0 98-99 22.993967888177885 31.0 2.0 34.0 2.0 35.0 100-101 19.8140961891502 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06746018593221947 1101 2 -0.08861550859860401 1101 3 -0.07793278618444077 1101 4 -0.027975830932156498 1101 5 -0.05237685279103488 1101 6 -0.08763655272051807 1101 7 -0.1621088477457704 1101 8 0.018305366910063015 1101 9 0.17494472296755958 1101 10-11 0.1923953739204336 1101 12-13 0.14468517896279565 1101 14-15 0.37998738434649937 1101 16-17 0.2960462510584847 1101 18-19 0.20055492909489914 1101 20-21 0.3003358333386146 1101 22-23 0.26571323033850547 1101 24-25 0.24360626160329701 1101 26-27 0.23459753752012347 1101 28-29 0.22192968774947275 1101 30-31 0.22017077098505666 1101 32-33 0.28191447035684547 1101 34-35 0.40259067875398813 1101 36-37 0.36742524401460486 1101 38-39 0.43270285035456624 1101 40-41 0.5192148502481047 1101 42-43 0.46609346473029234 1101 44-45 0.636024866434596 1101 46-47 0.3211583484768781 1101 48-49 0.5503055105375481 1101 50-51 0.4057172363380701 1101 52-53 0.6930290174275839 1101 54-55 0.8254662312180443 1101 56-57 0.9220783249884406 1101 58-59 0.9213763753859823 1101 60-61 1.0213748116472985 1101 62-63 1.0203197120908598 1101 64-65 1.055795647501533 1101 66-67 0.9488331632781595 1101 68-69 1.1865560662264585 1101 70-71 1.0500303481911004 1101 72-73 1.4297617678032246 1101 74-75 1.5208577388213023 1101 76-77 1.4132047157297976 1101 78-79 1.7075971875327483 1101 80-81 1.9270411784901142 1101 82-83 1.9971848166131565 1101 84-85 1.8480786231638646 1101 86-87 1.9354364084607454 1101 88-89 2.005594385795032 1101 90-91 2.0369541872074386 1101 92-93 1.7722157806595789 1101 94-95 2.289124977895227 1101 96-97 2.171811742255745 1101 98-99 2.2802285834474176 1101 100-101 2.896703305457155 1102 1 -0.09020264170663239 1102 2 -0.023164504249233886 1102 3 -0.10182372873430268 1102 4 -0.07933942483766998 1102 5 -0.28848833669857754 1102 6 -0.10954767781298358 1102 7 0.0674522748236086 1102 8 -0.07907180442037998 1102 9 0.03172897976835287 1102 10-11 0.11750910069059728 1102 12-13 -0.07832880322957436 1102 14-15 0.05219285160595888 1102 16-17 0.08707941729551294 1102 18-19 0.045331442705069946 1102 20-21 0.06965220580627118 1102 22-23 0.022685269174708367 1102 24-25 -0.06296535807849324 1102 26-27 0.02924945361623088 1102 28-29 0.1399916568948143 1102 30-31 0.3761039424829775 1102 32-33 0.3073375167633827 1102 34-35 0.20030841304402003 1102 36-37 0.008196814523060425 1102 38-39 0.3615281980304772 1102 40-41 0.35718600976191084 1102 42-43 0.3141269068963197 1102 44-45 0.5434270646332209 1102 46-47 0.6439346237359231 1102 48-49 0.6003732044045123 1102 50-51 0.4918307065049916 1102 52-53 0.6740978182315374 1102 54-55 0.5682134319343888 1102 56-57 0.6370635246719303 1102 58-59 0.5912885183021714 1102 60-61 0.7757464611766594 1102 62-63 0.8807339921147772 1102 64-65 0.9508798165848091 1102 66-67 1.0467133413758418 1102 68-69 1.0908317847960518 1102 70-71 1.074562798837917 1102 72-73 0.8750505216026134 1102 74-75 0.8569360468289311 1102 76-77 0.7306443951797412 1102 78-79 1.005315458649637 1102 80-81 1.4898075117157248 1102 82-83 1.687783045728711 1102 84-85 1.5517883114929276 1102 86-87 1.359340664316722 1102 88-89 1.485463378568273 1102 90-91 1.51786999235647 1102 92-93 1.5812224469755165 1102 94-95 1.7644615414947147 1102 96-97 1.84976286739116 1102 98-99 2.028475038511253 1102 100-101 2.539853018952659 1103 1 -0.15702925514796462 1103 2 -0.27581465017407325 1103 3 -0.1041649085496772 1103 4 0.048447797600523757 1103 5 0.06349803978170598 1103 6 -0.2182631330810736 1103 7 -0.18720832663041165 1103 8 -0.20670817016662824 1103 9 -0.2722196941433026 1103 10-11 -0.0668421285013352 1103 12-13 -0.08104930059248971 1103 14-15 -0.1916858480093495 1103 16-17 -0.09851420378728903 1103 18-19 0.03246728335398075 1103 20-21 -0.0634519897504191 1103 22-23 -0.13222714691125503 1103 24-25 -0.16993218121063336 1103 26-27 -0.15510757775616213 1103 28-29 -0.24119854438282573 1103 30-31 -0.0851461953188064 1103 32-33 -0.11368610391327394 1103 34-35 -0.1688205056036125 1103 36-37 -0.30281964046072574 1103 38-39 -0.0780957626539518 1103 40-41 0.06431877525518104 1103 42-43 -0.22497616303736834 1103 44-45 0.3046738661812398 1103 46-47 -0.10402881092282001 1103 48-49 -0.1691199461214481 1103 50-51 -0.0687411564010958 1103 52-53 0.09627679809216971 1103 54-55 0.2570642520725954 1103 56-57 0.1298767989003835 1103 58-59 0.37247523827154083 1103 60-61 0.1949344170104652 1103 62-63 0.4662595826317002 1103 64-65 0.43623221201502105 1103 66-67 0.6706276083573712 1103 68-69 0.5908042244388696 1103 70-71 0.7632032899344736 1103 72-73 0.6235071416004097 1103 74-75 0.518491234268371 1103 76-77 0.6399780727757829 1103 78-79 0.7143520646061567 1103 80-81 0.9149180432534756 1103 82-83 0.9228699914028979 1103 84-85 0.8193943028244703 1103 86-87 0.5453941082009308 1103 88-89 0.874793313757916 1103 90-91 1.172589485942158 1103 92-93 0.858407838245558 1103 94-95 0.700971907090107 1103 96-97 0.7775561821188326 1103 98-99 1.1697008361660117 1103 100-101 1.8084577105957784 1104 1 0.06992061274483774 1104 2 0.0449999536759087 1104 3 -0.04393603712257743 1104 4 0.025380508714683003 1104 5 0.038792278535439095 1104 6 0.09441915769200904 1104 7 0.2804425672377491 1104 8 0.2176047008487103 1104 9 0.26598845189519693 1104 10-11 0.14956489891519453 1104 12-13 -0.007163633365372846 1104 14-15 0.24028752467018677 1104 16-17 0.30905794046690005 1104 18-19 0.0250383982014597 1104 20-21 0.08026176655469897 1104 22-23 -0.012824545943615817 1104 24-25 0.010407008303147336 1104 26-27 0.11871460938998268 1104 28-29 -0.09960365024014806 1104 30-31 0.02068968293669826 1104 32-33 0.11496657425987422 1104 34-35 0.20172302114380614 1104 36-37 0.3197183618039716 1104 38-39 0.1657449538131246 1104 40-41 0.49455502409710306 1104 42-43 0.36818514499543653 1104 44-45 0.5620255458298686 1104 46-47 0.5238792630572036 1104 48-49 0.42109897437403987 1104 50-51 0.30191817860217185 1104 52-53 0.5443089155817873 1104 54-55 0.6757968808342198 1104 56-57 0.7273101448153554 1104 58-59 0.825594340628875 1104 60-61 0.817468130080492 1104 62-63 0.5551831663396243 1104 64-65 0.8443791083537633 1104 66-67 0.54999400852207 1104 68-69 0.5146023920949148 1104 70-71 0.6510665847222441 1104 72-73 0.7543833382857237 1104 74-75 0.8536145915068154 1104 76-77 0.8712171353293954 1104 78-79 0.9486579013422514 1104 80-81 0.8957453431196569 1104 82-83 1.0641346175566895 1104 84-85 1.293770653920788 1104 86-87 1.3645745288851465 1104 88-89 1.1991164788404411 1104 90-91 1.331860151282033 1104 92-93 1.1247925618498549 1104 94-95 1.5819038633060067 1104 96-97 1.4177956907486937 1104 98-99 1.885524533108672 1104 100-101 2.38307254091524 1105 1 -0.19526660070396318 1105 2 0.0711238711491653 1105 3 -0.15403537487092223 1105 4 -0.17539636341980014 1105 5 -0.22714315192813928 1105 6 -0.25039744954181486 1105 7 -0.16328196154472607 1105 8 -0.2852130525899028 1105 9 -0.25276473200699456 1105 10-11 -0.22416949996406998 1105 12-13 -0.2604908111544688 1105 14-15 -0.2308822409946103 1105 16-17 -0.4271000447597899 1105 18-19 -0.20908188962329888 1105 20-21 -0.15368340664957714 1105 22-23 -0.24534492291763144 1105 24-25 -0.10812967025008646 1105 26-27 -0.3330731516899945 1105 28-29 -0.3656505172803932 1105 30-31 -0.2961802228197996 1105 32-33 0.166187600749943 1105 34-35 -0.22874851477060787 1105 36-37 -0.11047458266877896 1105 38-39 -0.09435597588630884 1105 40-41 -0.0584330681087053 1105 42-43 -0.2886220378879045 1105 44-45 -0.13155570735403188 1105 46-47 -0.06915435894992328 1105 48-49 0.0534537884341475 1105 50-51 -0.17149623810694692 1105 52-53 0.023040448949082304 1105 54-55 -0.07106697550810992 1105 56-57 -0.08425856379391661 1105 58-59 0.185025058917212 1105 60-61 0.14015661709017735 1105 62-63 -0.0985197119079615 1105 64-65 0.08028238140521182 1105 66-67 0.43881214402895097 1105 68-69 0.18808526270112225 1105 70-71 0.060134338160857226 1105 72-73 0.2575433612098159 1105 74-75 0.22761962207227882 1105 76-77 0.3183484394557361 1105 78-79 0.5879695416886577 1105 80-81 0.5437729157126228 1105 82-83 0.4574579746631571 1105 84-85 0.49782694531151606 1105 86-87 0.3971776873161197 1105 88-89 0.8742390590747746 1105 90-91 0.9354245297843278 1105 92-93 1.1417627605253493 1105 94-95 0.8288552769585777 1105 96-97 1.0346369413854752 1105 98-99 1.3075109696853389 1105 100-101 1.9524020111139144 1106 1 -0.5036210456602035 1106 2 -0.27032304271541463 1106 3 -0.5140653149906811 1106 4 -0.5529297581687658 1106 5 -0.5854889603113733 1106 6 -0.3375116826141351 1106 7 -0.5258884792784855 1106 8 -0.3108780687115029 1106 9 -0.5484522032184174 1106 10-11 -0.8460531365370798 1106 12-13 -0.761570382780441 1106 14-15 -0.9891013796403954 1106 16-17 -0.5816011387257021 1106 18-19 -0.6243499112723114 1106 20-21 -0.2898605715505411 1106 22-23 -0.42374773268272037 1106 24-25 -0.796320596090716 1106 26-27 -0.7608210213353246 1106 28-29 -0.5975712911173403 1106 30-31 -0.09551664612703092 1106 32-33 -0.13237153098658183 1106 34-35 -0.500620635912945 1106 36-37 -0.8039482123325215 1106 38-39 -0.5372034540069919 1106 40-41 -0.4829998000294822 1106 42-43 -0.3483007573720087 1106 44-45 -0.3586975774599779 1106 46-47 -0.3115541747030335 1106 48-49 -0.13191585689058627 1106 50-51 -0.4202583293091564 1106 52-53 -0.3616325128196891 1106 54-55 -0.16827160471124358 1106 56-57 -0.5368221865484131 1106 58-59 -1.018251081110428 1106 60-61 -0.5495313147384735 1106 62-63 -0.561454419416016 1106 64-65 0.09400733534349115 1106 66-67 -0.040287349292100316 1106 68-69 -0.3410703683444751 1106 70-71 -0.46416322056784054 1106 72-73 -0.7111706530693169 1106 74-75 0.03851465892165251 1106 76-77 0.299707836047169 1106 78-79 0.27781581068911976 1106 80-81 -0.012515948865910786 1106 82-83 -0.16597304790708733 1106 84-85 -0.2547928151675265 1106 86-87 0.25634397013509513 1106 88-89 0.31256673065469087 1106 90-91 0.9993711557631393 1106 92-93 0.5553106647169663 1106 94-95 0.24000653214052292 1106 96-97 0.7317307345683375 1106 98-99 0.8976839890312647 1106 100-101 1.404684945245652 1107 1 -0.11492673616598736 1107 2 0.13255045557103173 1107 3 0.052725631418383045 1107 4 0.01806033711237376 1107 5 0.28484584178255545 1107 6 0.24180138935513895 1107 7 0.18167752079683197 1107 8 0.0965146929350098 1107 9 0.1362350260965357 1107 10-11 0.19746360356761272 1107 12-13 0.2487768472877221 1107 14-15 0.35881599316117274 1107 16-17 0.3609759712053773 1107 18-19 0.5240231539180655 1107 20-21 0.1543641864805565 1107 22-23 0.20455227108333673 1107 24-25 0.07967270033530127 1107 26-27 0.4280465265876998 1107 28-29 0.16325611068283763 1107 30-31 -0.2907299930181537 1107 32-33 0.33924184603296226 1107 34-35 0.3313098427523684 1107 36-37 -0.010949304487837708 1107 38-39 0.21718399750508155 1107 40-41 0.5346441573388034 1107 42-43 0.25960607048171624 1107 44-45 0.4365986225927543 1107 46-47 0.5415132753026128 1107 48-49 0.5579591101564532 1107 50-51 0.2500990691015659 1107 52-53 0.3016447063269254 1107 54-55 0.47899047490310664 1107 56-57 0.7336463104199211 1107 58-59 0.5530402983947127 1107 60-61 0.956889805285634 1107 62-63 0.8138986480339909 1107 64-65 0.8610863847917614 1107 66-67 1.09417015080205 1107 68-69 1.4724723575245378 1107 70-71 1.5221565926359375 1107 72-73 1.2484949214420347 1107 74-75 1.4251658847718396 1107 76-77 1.2621226291025636 1107 78-79 1.5401496336093174 1107 80-81 1.239913155190127 1107 82-83 1.5395969441842041 1107 84-85 1.729352102544219 1107 86-87 1.8100949587239548 1107 88-89 1.7826736865542152 1107 90-91 1.7779272511195963 1107 92-93 2.021086659896419 1107 94-95 2.4687848246121824 1107 96-97 2.754044597408697 1107 98-99 2.1399447505271425 1107 100-101 2.6271268538812116 1108 1 -0.17495365718991351 1108 2 -0.012904300863326057 1108 3 0.08166573214014505 1108 4 -0.4117361306636269 1108 5 -0.2828396463930858 1108 6 -0.21736452305870557 1108 7 -0.10167785992678091 1108 8 -0.3591468448124431 1108 9 0.00967140196973304 1108 10-11 -0.09494281106256608 1108 12-13 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-1.6099207718793167 2316 74-75 -1.1764363913587381 2316 76-77 -1.5763020489447541 2316 78-79 -1.3988665388974226 2316 80-81 -1.3475007411860318 2316 82-83 -1.8379861645309816 2316 84-85 -1.8801492451646737 2316 86-87 -2.0535243883859593 2316 88-89 -1.8646383829276694 2316 90-91 -2.1847486997639045 2316 92-93 -2.0120070807444925 2316 94-95 -1.5951564751781717 2316 96-97 -1.415130305256774 2316 98-99 -1.8840145187151478 2316 100-101 -1.8605940206321172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1943.0 3 318.0 4 325.0 5 396.0 6 403.0 7 480.0 8 544.0 9 737.0 10 907.0 11 1194.0 12 1557.0 13 2043.0 14 2351.0 15 3040.0 16 3684.0 17 5056.0 18 4329.0 19 3063.0 20 3088.0 21 3122.0 22 3480.0 23 3832.0 24 4307.0 25 4850.0 26 5585.0 27 6503.0 28 7731.0 29 9279.0 30 11114.0 31 13923.0 32 17820.0 33 22995.0 34 31167.0 35 43839.0 36 63511.0 37 85143.0 38 58649.0 39 5101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70993978115591 19.040767055886025 15.329872585738416 22.91942057721965 2 14.687540853514491 19.618631725491905 42.119772756984375 23.57405466400923 3 18.830148605348324 24.206914426347577 30.35709780337487 26.60583916492923 4 12.215466686237589 16.458381591712325 38.30297164586109 33.02318007618899 5 13.476278502263666 36.74710677082139 35.020572545170154 14.75604218174479 6 31.171250865123273 36.18209068783602 18.18798498466841 14.458673462372293 7 27.707607699358388 32.83913840513291 21.174839596700277 18.278414298808432 8 25.572623169840202 34.997673986923914 20.229438846480846 19.20026399675504 9 25.44602445256729 17.135130799463738 21.142876786103056 36.27596796186591 10-11 24.300536836617184 26.669836886462207 27.93635879249002 21.09326748443059 12-13 25.295725261692365 23.981161587514396 28.50375565066433 22.21935750012891 14-15 22.109535327933376 25.21716512570426 27.283685659711043 25.38961388665132 16-17 21.408029844384817 28.624165610671014 27.70401721368387 22.2637873312603 18-19 21.03091224460336 26.989155166132406 30.626287437360943 21.35364515190329 20-21 21.80762947399293 26.127133459658737 30.682752916215094 21.382484150133237 22-23 21.391662809402252 26.198310747331615 30.534066901005026 21.875959542261107 24-25 21.31271503648531 26.191744661252013 30.739433352008493 21.75610695025418 26-27 21.250488400418448 26.557868431515665 30.442270211183768 21.74937295688212 28-29 20.94213360834193 26.60765440586685 30.760627936289676 21.689584049501548 30-31 21.215308932273135 26.306139684438623 30.70884999661956 21.769701386668682 32-33 20.994300407703044 26.489009893502192 30.51115282103464 22.005536877760132 34-35 21.438246126273956 26.49420058349395 30.322090494273922 21.74546279595817 36-37 21.42040402929396 26.43078431911715 30.21615292252133 21.932658729067562 38-39 21.264033359299823 26.49452412592219 30.355588140952207 21.88585437382578 40-41 21.44566443860222 26.470780280770484 30.315995669879893 21.7675596107474 42-43 21.327792843419292 26.4638590726668 30.01522603617816 22.19312204773574 44-45 21.27524529235733 26.78578793725282 29.873996947880244 22.06496982250961 46-47 21.31652247043605 26.812020133620695 29.364929322650095 22.50652807329316 48-49 21.523476666571177 26.889964763514477 29.348497436044347 22.238061133869998 50-51 21.4511525368185 27.162904321740932 29.086988256350498 22.29895488509007 52-53 21.571813792621334 27.198695388592903 28.89948051620622 22.33001030257954 54-55 21.42482303146133 27.052754842716276 28.971966900180345 22.55045522564205 56-57 21.525949852811475 27.43032885467853 28.832915249189604 22.21080604332039 58-59 21.57325991804912 27.097385954746073 28.96258156723614 22.366772559968666 60-61 21.605056349014536 26.89793491943677 29.271248429340663 22.225760302208027 62-63 21.476299447258167 26.948626850012943 29.233583246860047 22.341490455868847 64-65 21.462288735016006 26.757730394094033 29.254823800278345 22.525157070611616 66-67 21.40090077706913 27.134878308041934 28.953270471373067 22.51095044351587 68-69 21.315936869667407 27.056609522238677 29.129684291066617 22.4977693170273 70-71 21.369353065151653 26.86406121139925 29.024443324475396 22.7421423989737 72-73 21.317417403656695 26.80680932960638 28.99830454108051 22.877468725656417 74-75 21.230531986315963 26.90085949117939 28.824460784426048 23.0441477380786 76-77 21.3424973305409 26.894170267954117 28.846083342116962 22.917249059388016 78-79 21.336249364170857 26.813086853115447 28.996329375535584 22.854334407178108 80-81 21.04706643831301 27.01454322585819 29.010499304055948 22.927891031772855 82-83 21.402275309728026 26.936487427607947 28.791419250788064 22.86981801187596 84-85 21.402342506202167 26.718567099081188 28.65034441209417 23.22874598262248 86-87 21.27037364132769 26.733699655098555 28.764735058184222 23.231191645389533 88-89 21.426108394135916 26.42916141123854 28.91350395861186 23.23122623601369 90-91 21.161728284751387 26.487070397430344 29.203466833455906 23.147734484362363 92-93 21.215005727376862 26.44306987399771 29.137915234822454 23.204009163802976 94-95 21.007541844407076 26.637922739623093 28.910556291208668 23.443979124761167 96-97 20.890480009164854 26.743727803872154 28.91224653454004 23.453545652422957 98-99 21.108187278540687 26.588750410984957 28.694368102755973 23.608694207718383 100-101 21.461425164980405 25.94813625399996 29.08064512434517 23.509793456674462 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 606.0 1 352.0 2 92.0 3 88.5 4 80.5 5 70.0 6 101.0 7 211.5 8 366.5 9 456.5 10 446.0 11 434.0 12 484.5 13 530.5 14 571.0 15 642.5 16 686.5 17 707.5 18 745.0 19 811.5 20 904.5 21 1034.0 22 1218.5 23 1508.5 24 1875.5 25 2259.0 26 2786.5 27 3489.0 28 4181.0 29 4866.0 30 5594.5 31 6292.5 32 7120.5 33 8012.5 34 9011.5 35 10086.5 36 10838.5 37 11672.0 38 12637.5 39 13290.5 40 13862.5 41 14624.5 42 15291.0 43 15838.0 44 16364.5 45 16728.5 46 16705.0 47 16793.0 48 17756.0 49 17949.5 50 17158.5 51 16170.0 52 14970.5 53 14126.5 54 13361.5 55 12233.5 56 10850.0 57 9448.5 58 8244.5 59 7157.5 60 5985.5 61 4810.0 62 3650.5 63 2692.0 64 1982.0 65 1519.5 66 1162.5 67 834.0 68 600.0 69 415.0 70 300.5 71 236.5 72 191.0 73 150.0 74 108.0 75 89.0 76 63.0 77 35.0 78 23.0 79 14.0 80 10.0 81 8.5 82 10.0 83 7.5 84 1.5 85 3.0 86 4.0 87 4.0 88 3.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38065060389703914 2 0.32006657384736026 3 0.3250961914363902 4 0.31732314607152573 5 0.31800900301548435 6 0.2411930252921179 7 0.2306765521514189 8 0.23776374057232477 9 0.24096440631079835 10-11 0.24153595376409723 12-13 0.24382214357729265 14-15 0.23947838293222135 16-17 0.23136240909537756 18-19 0.22404660169315216 20-21 0.22176041187995676 22-23 0.22930483826350168 24-25 0.2287332908102028 26-27 0.23673495515638682 28-29 0.24210750121739605 30-31 0.2458797144091685 32-33 0.2427933581613547 34-35 0.24507954797455014 36-37 0.22976207622614075 38-39 0.21695941327224635 40-41 0.21295858109915433 42-43 0.22530400609040965 44-45 0.22656141048766715 46-47 0.23422014636187183 48-49 0.24496523848389035 50-51 0.23902114496958224 52-53 0.25388137875535255 54-55 0.23593478872176843 56-57 0.20438536929967147 58-59 0.1842668989435517 60-61 0.203127964902414 62-63 0.1961550859721679 64-65 0.20610001165956804 66-67 0.2050712262436301 68-69 0.2019848699958163 70-71 0.20324227439307377 72-73 0.21695941327224635 74-75 0.22587555354370853 76-77 0.22656141048766715 78-79 0.2219890308612763 80-81 0.2175309607255452 82-83 0.2050712262436301 84-85 0.19844127578536336 86-87 0.20747172554748528 88-89 0.21844543665082336 90-91 0.21261565262717502 92-93 0.20781465401946464 94-95 0.2082718919821037 96-97 0.21924560308544178 98-99 0.21890267461346247 100-101 0.19249718227105525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 437409.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.19949915861197 #Duplication Level Percentage of deduplicated Percentage of total 1 87.53248166247204 66.69931262790756 2 8.294365063511226 12.640529273564882 3 1.9235952022139098 4.397309729778264 4 0.7705630685132964 2.3486607956334558 5 0.39648578336361406 1.510600905790866 6 0.2438765219002399 1.1149961295205524 7 0.15524346933520602 0.8280632227691623 8 0.1132645027328594 0.6904558704554499 9 0.08907168708594966 0.6108496150645771 >10 0.4501160578162874 5.987283653803542 >50 0.022827287127848442 1.19585663825256 >100 0.0069082577900990046 0.7870910826177915 >500 6.007180687042602E-4 0.382216373356124 >1k 6.007180687042602E-4 0.8067740814852318 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1998 0.45678072467644704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1527 0.34910118447494226 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 984 0.22496107761843034 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 686 0.15683262118520652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2861898131954303E-4 0.0 12-13 0.0 0.0 0.0 2.2861898131954303E-4 0.0 14-15 2.2861898131954303E-4 0.0 0.0 3.429284719793145E-4 0.0 16-17 2.2861898131954303E-4 0.0 0.0 4.5723796263908605E-4 0.0 18-19 2.2861898131954303E-4 0.0 0.0 4.5723796263908605E-4 0.0 20-21 2.2861898131954303E-4 0.0 0.0 4.5723796263908605E-4 0.0 22-23 2.2861898131954303E-4 0.0 0.0 8.001664346184007E-4 0.0 24-25 2.2861898131954303E-4 0.0 0.0 0.0011430949065977152 0.0 26-27 2.2861898131954303E-4 0.0 0.0 0.0012574043972574866 0.0 28-29 2.2861898131954303E-4 0.0 0.0 0.00297204675715406 0.0 30-31 2.2861898131954303E-4 0.0 0.0 0.005944093514308119 0.0 32-33 2.2861898131954303E-4 0.0 0.0 0.008458902308823093 0.0 34-35 2.2861898131954303E-4 0.0 0.0 0.010287854159379436 0.0 36-37 2.2861898131954303E-4 0.0 0.0 0.01291697244455418 0.0 38-39 2.2861898131954303E-4 0.0 0.0 0.01588901920170824 0.0 40-41 2.2861898131954303E-4 0.0 0.0 0.02171880322535659 0.0 42-43 2.2861898131954303E-4 0.0 0.0 0.030406324515499225 0.0 44-45 2.2861898131954303E-4 0.0 0.0 0.040808488165538434 0.0 46-47 2.2861898131954303E-4 0.0 0.0 0.0517821992688765 0.0 48-49 2.2861898131954303E-4 0.0 0.0 0.08687521290142636 0.0 50-51 2.2861898131954303E-4 0.0 0.0 0.12059651264605895 0.0 52-53 2.2861898131954303E-4 0.0 0.0 0.1604905248863192 0.0 54-55 2.2861898131954303E-4 0.0 0.0 0.23502031279649022 0.0 56-57 2.2861898131954303E-4 2.2861898131954303E-4 0.0 0.3040632451549923 0.0 58-59 2.2861898131954303E-4 2.2861898131954303E-4 0.0 0.38202231778495643 0.0 60-61 2.2861898131954303E-4 2.2861898131954303E-4 0.0 0.4740414577660724 0.0 62-63 2.2861898131954303E-4 2.2861898131954303E-4 0.0 0.5973813981879659 0.0 64-65 2.2861898131954303E-4 2.2861898131954303E-4 0.0 0.6816274928042175 0.0 66-67 2.2861898131954303E-4 2.2861898131954303E-4 0.0 0.8158268348387894 0.0 68-69 2.2861898131954303E-4 2.2861898131954303E-4 0.0 0.9922063789268167 0.0 70-71 2.2861898131954303E-4 2.2861898131954303E-4 0.0 1.1153177003673909 0.0 72-73 2.2861898131954303E-4 2.2861898131954303E-4 0.0 1.3311340187330394 0.0 74-75 2.2861898131954303E-4 2.2861898131954303E-4 0.0 1.6197654826489623 0.0 76-77 2.2861898131954303E-4 2.2861898131954303E-4 0.0 1.8323811352761374 0.0 78-79 2.2861898131954303E-4 2.2861898131954303E-4 0.0 2.0289934592109446 0.0 80-81 2.2861898131954303E-4 2.2861898131954303E-4 0.0 2.3177392326175275 0.0 82-83 2.2861898131954303E-4 2.2861898131954303E-4 0.0 2.6566668724237497 0.0 84-85 2.2861898131954303E-4 2.2861898131954303E-4 0.0 3.003367557594837 0.0 86-87 2.2861898131954303E-4 2.2861898131954303E-4 0.0 3.4942125104878956 0.0 88-89 2.2861898131954303E-4 2.2861898131954303E-4 0.0 4.10233900079788 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1140 0.0 43.397556 1 GTATCAA 1880 0.0 38.96724 1 TATCAAC 2370 0.0 30.692396 2 TCAACGC 2360 0.0 30.620993 4 ATCAACG 2360 0.0 30.617481 3 CAACGCA 2375 0.0 30.22395 5 AACGCAG 2455 0.0 29.415827 6 CCTACGT 115 3.830184E-6 28.93929 2 GTATAGC 175 4.8657967E-9 27.183083 1 CACGGAC 70 0.008353597 27.164381 3 ACGCAGA 2795 0.0 25.497547 7 CGCAGAG 2835 0.0 25.137794 8 ACGTCTA 95 0.0012196846 25.019827 5 CGTCTAT 100 0.0016498977 23.755215 6 GTACATG 1615 0.0 23.26973 1 GCAGAGT 2940 0.0 23.267748 9 GAGTACT 1625 0.0 22.504896 12-13 TACATGG 1610 0.0 22.442713 2 ACATGGG 1595 0.0 21.757051 3 CATGGGG 785 0.0 21.197567 4 >>END_MODULE