FastQCFastQC Report
Thu 26 May 2016
SRR937752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437409
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.47552748114464954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.3891095062058622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.27319968267685396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT156.6107325E-495.016018
TACCGTA156.6107325E-495.016017
GGTATCA13700.039.8881071
GTATCAA22600.035.744281
CTGTCGC600.00394504431.6720039
TCAACGC27300.029.4029944
CAACGCA27650.029.0308025
TATCAAC27750.028.9261882
ATCAACG27750.028.9261883
AACGCAG28250.028.4142196
GTGTAGC2350.028.3091141
TACTCCG700.00837909527.147437
ACGCAGA31800.025.0985687
CGCAGAG31900.025.0198888
TAGCCCT2351.7644197E-1024.253864
CCTACGT1000.001652592823.7485712
GTAGCCC2002.0480002E-823.7485713
GCAGAGT33450.023.434449
GTACATG18900.023.3823011
GAGTACT19500.022.41403412-13
Produced by FastQC (version 0.11.5)