##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937752_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 437409 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32481041770974 33.0 31.0 34.0 31.0 34.0 2 32.55349112615424 34.0 31.0 34.0 31.0 34.0 3 32.65471903870291 34.0 31.0 34.0 31.0 34.0 4 36.23331938757547 37.0 35.0 37.0 35.0 37.0 5 36.08471704971777 37.0 35.0 37.0 35.0 37.0 6 36.1741916604368 37.0 36.0 37.0 35.0 37.0 7 36.14162031416821 37.0 36.0 37.0 35.0 37.0 8 36.130552869282525 37.0 36.0 37.0 35.0 37.0 9 37.830947694263266 39.0 38.0 39.0 35.0 39.0 10-11 37.783156039313326 39.0 37.5 39.0 35.0 39.0 12-13 37.71356785068437 39.0 37.0 39.0 35.0 39.0 14-15 38.97394886707864 40.0 38.0 41.0 36.0 41.0 16-17 38.93481272676145 40.0 38.0 41.0 36.0 41.0 18-19 38.916406612575415 40.0 38.0 41.0 35.0 41.0 20-21 38.846864147742735 40.0 38.0 41.0 35.0 41.0 22-23 38.7045636921051 40.0 38.0 41.0 34.5 41.0 24-25 38.64626585186862 40.0 38.0 41.0 34.5 41.0 26-27 38.57159660637984 40.0 38.0 41.0 34.0 41.0 28-29 38.455255836071046 40.0 38.0 41.0 34.0 41.0 30-31 38.54623933206678 40.0 38.0 41.0 34.5 41.0 32-33 38.47317613492177 40.0 38.0 41.0 34.5 41.0 34-35 38.352954557405084 40.0 38.0 41.0 34.0 41.0 36-37 38.19625682141886 40.0 38.0 41.0 34.0 41.0 38-39 38.06145621146341 40.0 38.0 41.0 34.0 41.0 40-41 37.870547931112526 40.0 37.5 41.0 33.0 41.0 42-43 37.67572455070655 40.0 37.0 41.0 33.0 41.0 44-45 37.37259178480552 40.0 37.0 41.0 33.0 41.0 46-47 36.99494637741793 40.0 37.0 41.0 31.5 41.0 48-49 36.66159704075591 40.0 36.5 41.0 31.0 41.0 50-51 36.40950117624466 39.5 36.0 41.0 30.0 41.0 52-53 36.19849500124597 39.0 36.0 41.0 30.0 41.0 54-55 35.99062319248118 39.0 35.5 40.5 29.5 41.0 56-57 35.72017150995978 39.0 35.0 40.0 28.5 41.0 58-59 35.464174262532325 38.5 35.0 40.0 28.0 41.0 60-61 35.138996911357566 38.0 34.5 40.0 28.0 41.0 62-63 34.76955549611462 38.0 34.0 40.0 27.0 41.0 64-65 34.38186114140312 37.0 34.0 40.0 26.0 41.0 66-67 33.96791332597181 37.0 34.0 39.5 26.0 41.0 68-69 33.52367921099017 36.0 33.0 39.0 25.0 40.0 70-71 33.114917617150084 36.0 33.0 39.0 24.5 40.0 72-73 32.66818812598734 35.0 32.0 38.0 23.5 40.0 74-75 32.17690308155525 35.0 32.0 37.0 22.0 39.0 76-77 30.934741854877245 34.0 30.5 36.0 20.5 38.5 78-79 31.487963210633524 34.5 31.0 36.0 21.0 39.0 80-81 31.289760841683645 35.0 31.0 36.0 20.0 37.5 82-83 30.95257756470489 34.0 31.0 35.5 19.5 37.0 84-85 30.570704992352695 34.0 31.0 35.0 18.0 37.0 86-87 30.156007306662644 34.0 30.5 35.0 15.0 36.0 88-89 29.739843030207425 34.0 30.0 35.0 8.5 36.0 90-91 29.2710243730696 34.0 29.5 35.0 4.5 35.5 92-93 28.841046937763053 34.0 29.0 35.0 2.0 35.0 94-95 28.371893353817594 34.0 29.0 35.0 2.0 35.0 96-97 27.8642186146147 33.5 28.5 35.0 2.0 35.0 98-99 27.106234668239566 33.0 27.0 35.0 2.0 35.0 100-101 24.747100539769413 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12314714384461922 1101 2 0.12523149965761604 1101 3 0.09066618537956828 1101 4 0.011349755128193806 1101 5 -0.0695671949270178 1101 6 -0.008364225280054427 1101 7 -0.10130447274382703 1101 8 0.01625010370933211 1101 9 0.21378193402994583 1101 10-11 0.09024193067627806 1101 12-13 0.10044152172659437 1101 14-15 0.10108360433430619 1101 16-17 -0.16735507691317508 1101 18-19 0.1338465610136339 1101 20-21 0.14924113055170096 1101 22-23 0.11718987606647602 1101 24-25 0.06907542081422235 1101 26-27 0.12088010871639199 1101 28-29 0.07103269951870317 1101 30-31 0.09710150776734139 1101 32-33 0.08325429043642174 1101 34-35 -0.0013875522907156324 1101 36-37 0.07144167889357789 1101 38-39 0.25531275802818243 1101 40-41 0.2884545578615416 1101 42-43 0.39089181626832215 1101 44-45 0.26262795125820304 1101 46-47 0.2284533464801939 1101 48-49 0.2708502992566011 1101 50-51 0.18325444095894028 1101 52-53 0.36265507292070254 1101 54-55 0.39508039472319467 1101 56-57 0.27405643825076 1101 58-59 0.4339054013918684 1101 60-61 0.5534126139839444 1101 62-63 0.6368862860300766 1101 64-65 0.6363656242541609 1101 66-67 0.6029860639717626 1101 68-69 0.5438887479810433 1101 70-71 0.49162894944592495 1101 72-73 0.5844225227095379 1101 74-75 0.41177433452867973 1101 76-77 0.45106778377905243 1101 78-79 0.4029026894640033 1101 80-81 0.5670918393180777 1101 82-83 0.590732256038752 1101 84-85 0.5528338817800744 1101 86-87 0.894535468940493 1101 88-89 0.8450789416125666 1101 90-91 0.5512422347109514 1101 92-93 0.7790143829470288 1101 94-95 0.7992922935635711 1101 96-97 1.0945461552878122 1101 98-99 1.4109163567989462 1101 100-101 2.018033917524594 1102 1 0.0030171029062060484 1102 2 0.0310978362115506 1102 3 -0.016163433290834917 1102 4 0.033072990819874803 1102 5 -0.15050887513066868 1102 6 -0.07872504808531744 1102 7 0.015370550527613602 1102 8 -0.043410686134762955 1102 9 0.1143014750644582 1102 10-11 -0.13177892182624618 1102 12-13 -0.07478305979002187 1102 14-15 0.011035730114990372 1102 16-17 -0.03131080372843087 1102 18-19 0.09815670092145723 1102 20-21 -0.016853636567446983 1102 22-23 -0.005243688925546053 1102 24-25 -0.2377807171030355 1102 26-27 -0.1481925443571015 1102 28-29 -0.29698358566090377 1102 30-31 -0.15738931732480665 1102 32-33 -0.09392058306033135 1102 34-35 -0.07527747486954439 1102 36-37 -0.1305201011813324 1102 38-39 -0.13596423496838383 1102 40-41 -0.23050133537003603 1102 42-43 0.12133172440650952 1102 44-45 0.22526050723045188 1102 46-47 0.24483944953592385 1102 48-49 0.044599251102148685 1102 50-51 0.27642825798042736 1102 52-53 0.1951146310692451 1102 54-55 0.4178638634621521 1102 56-57 0.2970707425392547 1102 58-59 0.4722353759784852 1102 60-61 0.3361866989894011 1102 62-63 0.3328468786439842 1102 64-65 0.43746805189258 1102 66-67 0.5298643406287127 1102 68-69 0.29047587122253304 1102 70-71 0.4248686243865265 1102 72-73 0.1921207272384251 1102 74-75 0.19349194427487504 1102 76-77 0.1271244469277093 1102 78-79 0.28214885571498627 1102 80-81 0.3210189962078758 1102 82-83 0.2297698757914084 1102 84-85 0.20538826502506424 1102 86-87 -0.021528430634237594 1102 88-89 0.22504123489396122 1102 90-91 0.4266737092991768 1102 92-93 0.6718830675816463 1102 94-95 0.4584727307338916 1102 96-97 0.36169857868530286 1102 98-99 0.4344631933319434 1102 100-101 1.366664847985117 1103 1 -0.1271881782384483 1103 2 -0.09634560703442219 1103 3 -0.12873008583886758 1103 4 -0.16562703064818862 1103 5 -0.23109681007662175 1103 6 -0.11267795758870847 1103 7 -0.1272223858978947 1103 8 -0.0329043878678732 1103 9 -0.03269966796509749 1103 10-11 -0.07296802068960773 1103 12-13 -0.1371826095408295 1103 14-15 -0.22191601315013543 1103 16-17 -0.2799835549483305 1103 18-19 -0.21772125601476233 1103 20-21 -0.21688090681560368 1103 22-23 -0.25874095319746004 1103 24-25 -0.3242890519352599 1103 26-27 -0.3925774842174974 1103 28-29 -0.3693476550781867 1103 30-31 -0.502510263776486 1103 32-33 -0.22558293677285235 1103 34-35 -0.37482954653807354 1103 36-37 -0.397006996956776 1103 38-39 -0.4585923035965678 1103 40-41 -0.5056204251239649 1103 42-43 -0.5274013694901782 1103 44-45 -0.4828818376627595 1103 46-47 -0.6856098132970487 1103 48-49 -0.8279703605579058 1103 50-51 -0.6980552029041718 1103 52-53 -0.7922351649841062 1103 54-55 -0.8553499352834137 1103 56-57 -0.6369882018120379 1103 58-59 -0.8657278556039429 1103 60-61 -0.8586454439299089 1103 62-63 -0.6761486768857807 1103 64-65 -0.9192717609290781 1103 66-67 -0.5728531105894561 1103 68-69 -1.1442561260620465 1103 70-71 -0.9248019568178947 1103 72-73 -0.6503072822075282 1103 74-75 -0.5849786299669262 1103 76-77 -0.8255449912471562 1103 78-79 -0.6595566372092563 1103 80-81 -0.43291995905151026 1103 82-83 -0.5364609987712541 1103 84-85 -0.5211245084752179 1103 86-87 -0.5657686060921705 1103 88-89 -0.3233881148556712 1103 90-91 -0.37375739270895636 1103 92-93 -0.35416437104579046 1103 94-95 -0.5616434289189058 1103 96-97 -0.2614280422988635 1103 98-99 -0.36159741624513586 1103 100-101 0.3355056883729368 1104 1 -0.11799214488566889 1104 2 -0.03597090298885064 1104 3 -0.2046659881138524 1104 4 -0.045967078000899164 1104 5 -0.17866569454287173 1104 6 -0.19270575157010938 1104 7 -0.0830179457235829 1104 8 -0.08391149077423421 1104 9 -0.17817199408113993 1104 10-11 -0.10753035126641208 1104 12-13 -0.14018504580339197 1104 14-15 -0.19808524761467083 1104 16-17 -0.16494306495073374 1104 18-19 -0.15132881186007552 1104 20-21 -0.136930318338095 1104 22-23 -0.06441898908943244 1104 24-25 -0.20818055246712674 1104 26-27 -0.006181094003729015 1104 28-29 -0.03037260103433681 1104 30-31 0.14572430370112954 1104 32-33 -0.05317875738158051 1104 34-35 0.1392433011440417 1104 36-37 -0.09785711533854169 1104 38-39 -0.016333442120398445 1104 40-41 0.10242009918718509 1104 42-43 0.121180622754963 1104 44-45 0.010894540884400783 1104 46-47 0.2253936866857913 1104 48-49 0.1982065944570195 1104 50-51 0.2874558975316077 1104 52-53 0.431094622614971 1104 54-55 0.2605355260623625 1104 56-57 0.350002670781727 1104 58-59 0.5350630718915141 1104 60-61 0.37591345529116893 1104 62-63 0.5609408471851722 1104 64-65 0.4428863902564615 1104 66-67 0.49907668387233883 1104 68-69 0.2504098314577661 1104 70-71 0.25382155483156055 1104 72-73 0.20793912344734622 1104 74-75 0.5266905530272794 1104 76-77 0.053186608107751 1104 78-79 0.2254328971557733 1104 80-81 0.4539439533802927 1104 82-83 0.4951803902415435 1104 84-85 0.5068453299079287 1104 86-87 0.39217584025858443 1104 88-89 0.6437652324318393 1104 90-91 0.2757982467241824 1104 92-93 0.35834806761872784 1104 94-95 0.5008544264837624 1104 96-97 0.47031285603006623 1104 98-99 0.431959355535394 1104 100-101 1.077793716372799 1105 1 0.08402644910312063 1105 2 0.10451878116805347 1105 3 0.09665851519740443 1105 4 -0.057142574689642345 1105 5 0.007369710449481204 1105 6 -0.007880229410147876 1105 7 0.023833811790424875 1105 8 -0.04148599408549103 1105 9 -0.06933029769805898 1105 10-11 -0.01034969665612806 1105 12-13 -0.1267887773367562 1105 14-15 0.16647951295588115 1105 16-17 -0.1064707012217525 1105 18-19 -0.15805480523756188 1105 20-21 -0.1557073815067156 1105 22-23 -0.10887422342463537 1105 24-25 -0.2962711663234643 1105 26-27 -0.42055800434503965 1105 28-29 -0.37610458014793835 1105 30-31 -0.12404772890202764 1105 32-33 -0.411980654987282 1105 34-35 -0.36761800807654055 1105 36-37 -0.1882958570603961 1105 38-39 -0.3683297487938546 1105 40-41 -0.19189981930491484 1105 42-43 0.03988764007539203 1105 44-45 0.06084200649825533 1105 46-47 0.24058090013459577 1105 48-49 0.2498494879873263 1105 50-51 0.13992182977817436 1105 52-53 0.20120096494814277 1105 54-55 -0.2233937276309561 1105 56-57 0.013663203130171553 1105 58-59 0.1657463373015986 1105 60-61 0.4618071766360501 1105 62-63 0.36145345543784657 1105 64-65 0.3475364794057185 1105 66-67 0.4827591851458948 1105 68-69 0.7133253306936282 1105 70-71 0.7121363154021125 1105 72-73 0.4640124168757751 1105 74-75 0.5278526773268126 1105 76-77 0.266293141475046 1105 78-79 0.5602520515113518 1105 80-81 0.8024140883777413 1105 82-83 0.761696882854892 1105 84-85 0.9814537099384957 1105 86-87 0.714323063895872 1105 88-89 0.7289982940721877 1105 90-91 0.9298433944572473 1105 92-93 0.6886938393966204 1105 94-95 0.6934009114557433 1105 96-97 0.9469032151742347 1105 98-99 1.0820803438186353 1105 100-101 1.597549829465006 1106 1 -0.3419581202197719 1106 2 -0.1773933560958838 1106 3 -0.21920974827565232 1106 4 0.04877059896305269 1106 5 -0.13424129830224274 1106 6 -0.06836502904165798 1106 7 -0.2929556861367928 1106 8 -0.26996653300764706 1106 9 -0.28036495914439286 1106 10-11 -0.08440111305409914 1106 12-13 -0.22469152952469074 1106 14-15 -0.2211946579331041 1106 16-17 -0.37024603516923804 1106 18-19 -0.14673369745313636 1106 20-21 -0.17569385087567468 1106 22-23 -0.3941618213058007 1106 24-25 -0.5009939323299122 1106 26-27 -0.553827523000038 1106 28-29 -0.39093789428888925 1106 30-31 -0.2672708979485492 1106 32-33 -0.069130298008929 1106 34-35 -0.37482954653807354 1106 36-37 -0.14319855167117623 1106 38-39 -0.4303547372784351 1106 40-41 -0.3403912978862138 1106 42-43 -0.3198760057099648 1106 44-45 -0.32333435232255425 1106 46-47 -0.41139399622652917 1106 48-49 -0.09008602560087553 1106 50-51 -0.08918486068704823 1106 52-53 -0.16658749659032424 1106 54-55 -0.031395512522713886 1106 56-57 -0.6973066840186064 1106 58-59 -0.42264898743354706 1106 60-61 -0.31927728904796737 1106 62-63 -0.5977963142535359 1106 64-65 -0.5081535827776094 1106 66-67 -0.3341310727997495 1106 68-69 -0.7764619217246107 1106 70-71 -0.40551540039705714 1106 72-73 -0.41193040901091393 1106 74-75 -0.5258236239168461 1106 76-77 -0.3654793711919311 1106 78-79 -0.08299990611186203 1106 80-81 0.044101386857708036 1106 82-83 -0.2653283935154427 1106 84-85 -0.09974447062345604 1106 86-87 -0.12434575231785416 1106 88-89 0.1557313663890909 1106 90-91 0.3826805086903171 1106 92-93 0.5063038117595866 1106 94-95 0.2553107456335404 1106 96-97 0.2789290673161062 1106 98-99 0.7287581420682905 1106 100-101 1.6176874388615978 1107 1 -0.29614408790703806 1107 2 -0.11574307175889231 1107 3 -0.12818425209819395 1107 4 -0.04967402123052267 1107 5 -0.5900843292215399 1107 6 -0.16357084382718057 1107 7 -0.22785946348775354 1107 8 -0.39938693748171517 1107 9 -0.13121043799800702 1107 10-11 -0.45466059409202586 1107 12-13 -0.34494159543061187 1107 14-15 -0.6496867303919629 1107 16-17 -0.43117135434221154 1107 18-19 -0.5934911259922799 1107 20-21 -0.4764696577524745 1107 22-23 -0.16486569657413241 1107 24-25 -0.007000786546008442 1107 26-27 -0.4290017029420454 1107 28-29 -0.49842104120260444 1107 30-31 -0.28028261037276536 1107 32-33 -0.2069960758871332 1107 34-35 -0.47545847735568003 1107 36-37 -0.33589805831825714 1107 38-39 -0.40868110341408226 1107 40-41 0.10690279317694262 1107 42-43 -0.07388685194202793 1107 44-45 -0.21730113573686083 1107 46-47 -0.13614261220212143 1107 48-49 -0.5494973915482362 1107 50-51 -0.28376739373415916 1107 52-53 -0.3898579368418922 1107 54-55 -1.176972283509052 1107 56-57 -0.517609474663729 1107 58-59 -0.31654986492385007 1107 60-61 -0.5233691054152345 1107 62-63 -0.6344494231301212 1107 64-65 -0.3446880609909897 1107 66-67 -0.2749663833108045 1107 68-69 -0.33563602634555423 1107 70-71 -0.32513841851177006 1107 72-73 -0.3510804108939425 1107 74-75 -0.02997569712891135 1107 76-77 -0.5974964690716433 1107 78-79 -0.20776923537786374 1107 80-81 -0.2518538724350279 1107 82-83 -0.5354956062597687 1107 84-85 -0.62185120055981 1107 86-87 -0.21673644263533376 1107 88-89 -0.11646009973526716 1107 90-91 0.0418339000208654 1107 92-93 -0.0545179409613894 1107 94-95 -0.07974555685581919 1107 96-97 -0.09206479401782985 1107 98-99 -0.11303939493317827 1107 100-101 0.8361655769250902 1108 1 -0.012428986605080183 1108 2 0.014742667060758663 1108 3 -0.014965101038761475 1108 4 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-0.4273514896170951 2316 64-65 -0.7719117806675371 2316 66-67 -0.583441974805261 2316 68-69 -0.614761513018216 2316 70-71 -0.5719563280655322 2316 72-73 -0.33610586761751193 2316 74-75 -0.22752735930321677 2316 76-77 -0.29531018575328005 2316 78-79 -0.4186856774263781 2316 80-81 -0.3704148188261627 2316 82-83 -0.7821637703807589 2316 84-85 -0.6960874988527124 2316 86-87 -0.6933387187659115 2316 88-89 -0.6338680062941151 2316 90-91 -0.590016953528103 2316 92-93 -0.44557813862535767 2316 94-95 -0.09486984556501099 2316 96-97 0.007590791486808968 2316 98-99 0.1613691106074029 2316 100-101 0.24815269881787216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 13.0 6 16.0 7 39.0 8 96.0 9 186.0 10 316.0 11 498.0 12 823.0 13 1151.0 14 1566.0 15 2274.0 16 3167.0 17 4581.0 18 4155.0 19 2342.0 20 2028.0 21 1988.0 22 2075.0 23 2216.0 24 2377.0 25 2856.0 26 3473.0 27 4398.0 28 5466.0 29 6794.0 30 8477.0 31 11006.0 32 14054.0 33 18957.0 34 27112.0 35 40168.0 36 64429.0 37 103723.0 38 86734.0 39 7853.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.702331804718504 19.996340473222556 15.440000914881693 21.861326807177246 2 15.389258108543721 20.817815819976268 40.655999304998296 23.136926766481714 3 19.47216449592944 24.822077277788065 30.276697553091044 25.42906067319145 4 12.890909880683754 17.37115605760284 37.77082776074566 31.967106300967746 5 13.741370912973986 37.84450608123404 34.17221359662862 14.241909409163354 6 31.518327240637483 37.06713853624411 17.256160709999108 14.1583735131193 7 28.231243527225093 33.456558964264566 20.450653735977085 17.86154377253326 8 26.125662709272103 35.86528855144727 19.533663001904397 18.475385737376232 9 26.36571263965762 17.39493243166007 20.60794359512493 35.63141133355738 10-11 24.907466467310915 27.53349839623785 26.9579501107659 20.601085025685343 12-13 25.83533672395049 24.683944410340125 28.006086192094582 21.474632673614803 14-15 22.656712596220014 26.068507963942217 26.642570226035588 24.632209213802188 16-17 21.87997960718687 29.439723462480195 27.216060940675664 21.464235989657276 18-19 21.625526680978215 27.609171279054618 30.02430219771427 20.7409998422529 20-21 22.30980879429641 26.84332837610609 30.328057182244972 20.51880564735253 22-23 21.940842417148293 26.816610578681686 30.267279062111346 20.97526794205867 24-25 22.092607220057314 26.979823116483182 30.202454696032184 20.725114967427324 26-27 21.98779376748259 27.347653575278436 29.977286210889588 20.68726644634939 28-29 21.549252625755035 27.393700931417154 30.17903602668508 20.878010416142732 30-31 21.794158092492488 27.08977991968606 30.176939862851317 20.939122124970137 32-33 21.545281418535055 27.202915349249785 30.1597589441461 21.09204428806906 34-35 21.983086767761982 27.24360952792467 29.8971900441006 20.876113660212752 36-37 21.95885315574211 27.073631315313584 29.944857101705725 21.02265842723858 38-39 21.95759575134485 27.213774750862463 29.963718167664588 20.864911330128095 40-41 22.050186438779264 27.170908691865055 29.761733297668773 21.01717157168691 42-43 21.91742139644635 27.272134865886365 29.633842685428018 21.176601052239267 44-45 21.985144338593855 27.660267621379532 29.27694674778068 21.07764129224593 46-47 22.15809459796209 27.909805239489817 28.6185240815804 21.313576080967696 48-49 22.442953848686244 27.85196463721597 28.47243655251721 21.23264496158058 50-51 22.379169152898086 27.998623713732457 28.270908920484032 21.35129821288542 52-53 22.476675148430875 28.227585623523975 28.035545679215563 21.260193548829584 54-55 22.314469981184658 28.130193937481852 28.193292776326047 21.362043305007443 56-57 22.315841695072574 28.531763178169633 27.954385940847125 21.198009185910667 58-59 22.454613416733537 28.075096762983843 28.153055835613806 21.317233984668814 60-61 22.53417282223274 27.789665964806392 28.557368504077417 21.118792708883447 62-63 22.271603922187243 28.064351670861825 28.501242544163475 21.162801862787457 64-65 22.229766648605768 27.967531532273 28.233186788566307 21.569515030554925 66-67 22.125287774142734 28.128136366649976 28.292170485746748 21.454405373460535 68-69 22.180956496094044 28.141281958075854 28.194778799704622 21.48298274612548 70-71 22.28909327425819 28.12242089211699 28.095786780793265 21.49269905283156 72-73 22.474274649127018 27.924436854294264 27.919521546195895 21.681766950382823 74-75 22.32635816821327 28.059322053272794 27.84156247356593 21.772757304948 76-77 22.231252671984343 28.036574464631503 27.958729701492196 21.773443161891958 78-79 22.31915667030171 27.965473961441123 27.904432693428806 21.810936674828366 80-81 22.04220997077103 28.389366004547234 27.783525011516698 21.784899013165038 82-83 22.198585532745703 28.339184080784896 27.622691374310282 21.839539012159115 84-85 22.25788678330807 28.048348342169454 27.738455312990816 21.955309561531656 86-87 22.168268142630808 28.050177294020013 27.64094931745803 22.140605245891145 88-89 22.30692555480111 27.826473620798843 27.745542501411723 22.121058322988326 90-91 22.21909752707053 27.907566944688323 27.896935359964882 21.976400168276268 92-93 22.181590805690888 27.70901301094419 27.96815736589537 22.141238817469553 94-95 22.10871289799707 27.981134361661514 27.76554666227718 22.14460607806424 96-97 22.161295263700566 28.13179427035109 27.660610549851512 22.046299916096835 98-99 22.16372109824112 28.048380404130235 27.310283179224914 22.477615318403736 100-101 22.17293531360002 28.16929748244249 27.176953848018226 22.480813355939254 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 101.0 1 64.0 2 37.0 3 52.0 4 54.5 5 61.5 6 101.0 7 209.5 8 349.0 9 441.5 10 463.0 11 474.5 12 542.0 13 606.0 14 687.0 15 747.0 16 766.5 17 821.5 18 846.0 19 880.5 20 962.0 21 1078.5 22 1230.0 23 1512.0 24 1887.0 25 2256.0 26 2817.5 27 3517.0 28 4211.0 29 4962.0 30 5745.5 31 6522.0 32 7235.0 33 8198.0 34 9319.5 35 10130.0 36 10967.0 37 11829.5 38 12700.5 39 13291.5 40 13772.5 41 14555.5 42 15499.0 43 16248.5 44 16664.0 45 16972.5 46 16773.0 47 16904.5 48 17939.0 49 17734.0 50 16787.5 51 15954.5 52 14921.0 53 13979.0 54 12892.5 55 11804.0 56 10484.0 57 9170.0 58 8168.0 59 7286.5 60 6020.5 61 4641.5 62 3513.0 63 2626.0 64 1999.5 65 1490.0 66 1076.0 67 874.0 68 639.0 69 376.5 70 247.0 71 174.0 72 156.0 73 127.5 74 94.5 75 79.0 76 50.5 77 28.5 78 21.0 79 15.0 80 10.5 81 5.5 82 4.0 83 3.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04435208237599135 2 0.0 3 0.0 4 0.0 5 0.018289518505563443 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005829784023648347 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.1430949065977153E-4 22-23 2.2861898131954305E-4 24-25 5.715474532988576E-4 26-27 0.002171880322535659 28-29 0.0025148087945149737 30-31 1.1430949065977153E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01280266295389441 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1430949065977153E-4 82-83 1.1430949065977153E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0075444263835449206 92-93 0.0018289518505563444 94-95 0.0 96-97 0.0 98-99 1.1430949065977153E-4 100-101 0.013831448369832354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 437409.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.37020345028586 #Duplication Level Percentage of deduplicated Percentage of total 1 85.83306971904456 62.975897880488695 2 9.15668608182925 13.436558415084257 3 2.374618549219333 5.226787382191353 4 0.9171435628434806 2.691640391957848 5 0.4873068277885164 1.787690054877843 6 0.2878966975220634 1.267382356191552 7 0.17032548699000336 0.8747770943257894 8 0.12549946354910024 0.7366336938799375 9 0.0987621070861907 0.6521576301083458 >10 0.5121645212379703 6.72950792608194 >50 0.02497571489392812 1.23737758132078 >100 0.010614678698639874 1.243562166352719 >500 0.0 0.0 >1k 9.365892969388116E-4 1.1400274271389716 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2080 0.47552748114464954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1702 0.3891095062058622 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1195 0.27319968267685396 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 2.2861898131954303E-4 3 0.0 0.0 0.0 0.0 2.2861898131954303E-4 4 0.0 0.0 0.0 0.0 2.2861898131954303E-4 5 0.0 0.0 0.0 0.0 2.2861898131954303E-4 6 0.0 0.0 0.0 0.0 2.2861898131954303E-4 7 0.0 0.0 0.0 0.0 2.2861898131954303E-4 8 0.0 0.0 0.0 0.0 2.2861898131954303E-4 9 0.0 0.0 0.0 0.0 2.2861898131954303E-4 10-11 0.0 0.0 0.0 0.0 2.2861898131954303E-4 12-13 0.0 0.0 0.0 0.0 2.2861898131954303E-4 14-15 0.0 0.0 0.0 1.1430949065977151E-4 2.2861898131954303E-4 16-17 0.0 0.0 0.0 3.429284719793145E-4 2.2861898131954303E-4 18-19 0.0 0.0 0.0 4.5723796263908605E-4 2.2861898131954303E-4 20-21 0.0 0.0 0.0 5.715474532988576E-4 2.2861898131954303E-4 22-23 0.0 0.0 0.0 0.0010287854159379438 2.2861898131954303E-4 24-25 0.0 0.0 0.0 0.0013717138879172583 2.2861898131954303E-4 26-27 0.0 0.0 0.0 0.00148602337857703 2.2861898131954303E-4 28-29 0.0 0.0 0.0 0.0032006657384736027 2.2861898131954303E-4 30-31 0.0 0.0 0.0 0.00605840300496789 2.2861898131954303E-4 32-33 0.0 0.0 0.0 0.00823028332750355 2.2861898131954303E-4 34-35 0.0 0.0 0.0 0.010173544668719666 2.2861898131954303E-4 36-37 0.0 0.0 0.0 0.012688353463234638 2.2861898131954303E-4 38-39 0.0 0.0 0.0 0.0156604002203887 2.2861898131954303E-4 40-41 0.0 0.0 0.0 0.021604493734696816 2.2861898131954303E-4 42-43 0.0 0.0 0.0 0.030634943496818768 2.2861898131954303E-4 44-45 0.0 0.0 0.0 0.041494345109497066 2.2861898131954303E-4 46-47 0.0 0.0 0.0 0.052468056212835124 2.2861898131954303E-4 48-49 0.0 0.0 0.0 0.08721814137340567 2.2861898131954303E-4 50-51 0.0 0.0 0.0 0.12105375060869804 2.2861898131954303E-4 52-53 0.0 0.0 0.0 0.16117638183027783 2.2861898131954303E-4 54-55 0.0 0.0 0.0 0.23593478872176843 2.2861898131954303E-4 56-57 0.0 0.0 0.0 0.30749252987478537 2.2861898131954303E-4 58-59 0.0 0.0 0.0 0.387737792317945 2.2861898131954303E-4 60-61 0.0 0.0 0.0 0.47929969433642194 2.2861898131954303E-4 62-63 0.0 0.0 0.0 0.6025253252676557 2.2861898131954303E-4 64-65 0.0 0.0 0.0 0.6875715863185257 2.2861898131954303E-4 66-67 0.0 0.0 0.0 0.8239428086756331 2.2861898131954303E-4 68-69 0.0 0.0 0.0 1.0034087090114743 2.2861898131954303E-4 70-71 0.0 0.0 0.0 1.1280060538306254 2.2861898131954303E-4 72-73 0.0 0.0 0.0 1.345308395574851 2.2861898131954303E-4 74-75 0.0 0.0 0.0 1.6356545018506707 2.2861898131954303E-4 76-77 0.0 0.0 0.0 1.8520423676696183 2.2861898131954303E-4 78-79 0.0 0.0 0.0 2.0520839763242185 2.2861898131954303E-4 80-81 0.0 0.0 0.0 2.344373343941254 2.2861898131954303E-4 82-83 0.0 0.0 0.0 2.688444910827166 2.2861898131954303E-4 84-85 0.0 0.0 0.0 3.0401752135872835 2.2861898131954303E-4 86-87 0.0 0.0 0.0 3.5379930454105883 2.2861898131954303E-4 88-89 0.0 0.0 0.0 4.158007722749189 2.2861898131954303E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTAT 15 6.6107325E-4 95.01601 8 TACCGTA 15 6.6107325E-4 95.01601 7 GGTATCA 1370 0.0 39.888107 1 GTATCAA 2260 0.0 35.74428 1 CTGTCGC 60 0.003945044 31.672003 9 TCAACGC 2730 0.0 29.402994 4 CAACGCA 2765 0.0 29.030802 5 TATCAAC 2775 0.0 28.926188 2 ATCAACG 2775 0.0 28.926188 3 AACGCAG 2825 0.0 28.414219 6 GTGTAGC 235 0.0 28.309114 1 TACTCCG 70 0.008379095 27.14743 7 ACGCAGA 3180 0.0 25.098568 7 CGCAGAG 3190 0.0 25.019888 8 TAGCCCT 235 1.7644197E-10 24.25386 4 CCTACGT 100 0.0016525928 23.748571 2 GTAGCCC 200 2.0480002E-8 23.748571 3 GCAGAGT 3345 0.0 23.43444 9 GTACATG 1890 0.0 23.382301 1 GAGTACT 1950 0.0 22.414034 12-13 >>END_MODULE