FastQCFastQC Report
Thu 26 May 2016
SRR937751_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937751_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400187
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.242896127445834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28090.2006160605690526No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19640.14026697862499796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17850.12748297191732247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.048.079871
GTATCAA37800.045.0431481
ATCAACG49000.034.3408743
CAACGCA49700.033.8644755
TCAACGC49850.033.856744
TATCAAC50900.033.246962
AACGCAG52100.032.371416
ACGCAGA59350.027.778637
CGCAGAG59550.027.6833538
GTGGTAT10950.025.1913471
GCAGAGT65050.024.7575599
GTACATG43750.024.2417681
ACATGGG42750.022.7940713
TACATGG44300.022.641152
GAGTACT39850.022.11312-13
TGGTATC12100.021.214272
GTACTTT42500.021.06800814-15
CAGAGTA62350.020.91479310-11
GGACCGA3700.020.54426
AGAGTAC61300.020.53681410-11
Produced by FastQC (version 0.11.5)