##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937751_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1400187 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.470442876558632 31.0 31.0 34.0 30.0 34.0 2 31.626046378090926 33.0 31.0 34.0 30.0 34.0 3 31.64700143623673 33.0 31.0 34.0 30.0 34.0 4 35.24140775482132 37.0 35.0 37.0 33.0 37.0 5 35.217911607520996 37.0 35.0 37.0 33.0 37.0 6 35.26975753952865 37.0 35.0 37.0 33.0 37.0 7 35.19211148225202 37.0 35.0 37.0 32.0 37.0 8 35.16886601575361 37.0 35.0 37.0 32.0 37.0 9 36.77341883619831 39.0 37.0 39.0 33.0 39.0 10-11 36.631653486284335 39.0 37.0 39.0 32.0 39.0 12-13 36.494225057081664 39.0 37.0 39.0 32.0 39.0 14-15 37.62801218694361 40.0 37.0 41.0 32.0 41.0 16-17 37.52318368903582 40.0 37.0 41.0 32.0 41.0 18-19 37.415006352722884 40.0 37.0 41.0 32.0 41.0 20-21 37.28474160951359 40.0 37.0 41.0 32.0 41.0 22-23 37.18999962147913 40.0 37.0 41.0 31.0 41.0 24-25 37.226952185672346 40.0 37.0 41.0 31.0 41.0 26-27 37.135182300649845 40.0 37.0 41.0 31.0 41.0 28-29 36.99878694774341 39.5 36.5 41.0 31.0 41.0 30-31 36.76243101814258 39.0 36.0 41.0 30.0 41.0 32-33 36.63618109581077 39.0 36.0 41.0 30.0 41.0 34-35 36.63572865624377 39.0 36.0 41.0 30.0 41.0 36-37 36.49006989780651 39.0 36.0 41.0 30.0 41.0 38-39 36.33637828375781 39.0 35.5 41.0 30.0 41.0 40-41 36.10118826985253 39.0 35.0 41.0 29.5 41.0 42-43 35.8352630755749 39.0 35.0 40.0 28.0 41.0 44-45 35.61720327356275 39.0 35.0 40.0 27.0 41.0 46-47 35.311573739793324 38.5 35.0 40.0 26.0 41.0 48-49 35.01303004527252 38.0 34.0 40.0 24.5 41.0 50-51 34.13749520599748 37.5 33.5 39.5 23.5 40.5 52-53 34.22909689919989 38.0 33.5 39.5 24.0 40.5 54-55 34.49226924689345 38.0 34.0 40.0 24.0 41.0 56-57 34.22653759819224 38.0 34.0 40.0 23.0 41.0 58-59 33.94509661923729 38.0 33.0 40.0 22.5 41.0 60-61 33.59628856716996 37.5 33.0 40.0 21.5 41.0 62-63 33.17854150909842 37.0 32.5 40.0 19.5 41.0 64-65 32.756533234489396 36.0 32.0 39.0 18.0 41.0 66-67 32.290144816370955 36.0 31.0 39.0 15.0 40.5 68-69 31.758653665546102 35.0 31.0 39.0 10.5 40.0 70-71 31.26546168476068 35.0 30.5 38.0 8.0 40.0 72-73 30.77249074587894 35.0 30.0 37.0 7.0 39.5 74-75 30.20616567644179 34.0 29.0 37.0 2.0 39.0 76-77 29.692753896443833 34.0 29.0 36.0 2.0 39.0 78-79 29.11496214434215 34.0 28.5 36.0 2.0 37.5 80-81 28.530723753327237 34.0 27.5 35.0 2.0 37.0 82-83 27.98775913502982 33.5 26.0 35.0 2.0 36.5 84-85 27.438881377987368 33.0 26.0 35.0 2.0 36.0 86-87 26.865412262790613 32.5 25.0 35.0 2.0 36.0 88-89 26.355889963269192 32.0 24.5 35.0 2.0 35.0 90-91 25.753910013448206 32.0 22.0 34.5 2.0 35.0 92-93 25.06318370331963 31.5 19.0 34.0 2.0 35.0 94-95 24.26355372532383 31.0 12.0 34.0 2.0 35.0 96-97 23.370813327077027 31.0 2.0 34.0 2.0 35.0 98-99 22.2708952447066 30.5 2.0 34.0 2.0 35.0 100-101 19.498604472116938 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1167353796573316 1101 2 0.3130036962183915 1101 3 0.27759998353892 1101 4 0.14762252501191853 1101 5 0.17918001246378878 1101 6 0.2159408728924106 1101 7 0.1792003913526088 1101 8 0.34779251493821306 1101 9 0.364766999254428 1101 10-11 0.3135662115766493 1101 12-13 0.327510312506881 1101 14-15 0.505699620293683 1101 16-17 0.6291599085718147 1101 18-19 0.6527791747551461 1101 20-21 0.4650235071941822 1101 22-23 0.46886154168353755 1101 24-25 0.539135630602452 1101 26-27 0.35220356369561756 1101 28-29 0.5471559473895837 1101 30-31 0.5156373240377761 1101 32-33 0.4198617261585227 1101 34-35 0.4882429806999724 1101 36-37 0.5943390592797115 1101 38-39 0.5714517238040671 1101 40-41 0.6247542176659309 1101 42-43 0.5403763543569156 1101 44-45 0.7219598393613254 1101 46-47 0.8831485324592379 1101 48-49 0.9868540643709736 1101 50-51 0.9087925817632083 1101 52-53 0.8547607229110739 1101 54-55 0.9576419496561002 1101 56-57 0.71856089295229 1101 58-59 0.8413093119692618 1101 60-61 0.9750869955069774 1101 62-63 0.772050160342765 1101 64-65 0.7430148184077083 1101 66-67 0.7974186207568437 1101 68-69 0.8778790598483717 1101 70-71 0.9326168311436618 1101 72-73 0.9752198492695108 1101 74-75 1.0166187673145082 1101 76-77 0.8902271463470832 1101 78-79 0.9229785152481362 1101 80-81 1.1032672580165332 1101 82-83 1.100788360862687 1101 84-85 0.9797033726960187 1101 86-87 1.0567912150242726 1101 88-89 1.1981641529538685 1101 90-91 1.0826340751342265 1101 92-93 1.1676774857951493 1101 94-95 1.0677542050630713 1101 96-97 1.1473181920030413 1101 98-99 1.5316897541719818 1101 100-101 1.893699066579284 1102 1 0.22596308355703343 1102 2 0.2671876078457345 1102 3 0.31949046692515637 1102 4 0.09670348778113436 1102 5 0.1433604947937468 1102 6 0.07034862937467068 1102 7 0.016295260380942977 1102 8 0.10254710795392441 1102 9 0.17106137573725988 1102 10-11 0.21422366238842017 1102 12-13 0.2658196158386019 1102 14-15 0.4841030050246786 1102 16-17 0.47214776712269924 1102 18-19 0.4192869490350333 1102 20-21 0.5662888262900623 1102 22-23 0.4252269880637414 1102 24-25 0.43541313069771803 1102 26-27 0.304652810335412 1102 28-29 0.4207600521283368 1102 30-31 0.45699199779390653 1102 32-33 0.45521291080954285 1102 34-35 0.36213503066256436 1102 36-37 0.6234697589190787 1102 38-39 0.531177978090092 1102 40-41 0.4063986501702672 1102 42-43 0.5517938406867486 1102 44-45 0.6259286602473395 1102 46-47 0.42263392562987434 1102 48-49 0.6410058052337746 1102 50-51 0.43968495632737614 1102 52-53 0.3773477829324605 1102 54-55 0.4762694011093487 1102 56-57 0.3777490224707307 1102 58-59 0.4611678377765287 1102 60-61 0.4704733606012681 1102 62-63 0.6037150673441758 1102 64-65 0.5750918681122599 1102 66-67 0.7230851030163805 1102 68-69 0.5194362116697882 1102 70-71 0.5960267103175987 1102 72-73 0.5613458573792443 1102 74-75 0.7579248231836004 1102 76-77 0.5594231810953687 1102 78-79 0.6876780706935044 1102 80-81 0.5118781707064279 1102 82-83 0.8277203069141947 1102 84-85 0.8047228704944693 1102 86-87 0.6931381017190326 1102 88-89 0.9641367928520772 1102 90-91 0.7931225160868856 1102 92-93 0.753410161693747 1102 94-95 0.6810702970531288 1102 96-97 0.5545134945891022 1102 98-99 0.6566128843729011 1102 100-101 0.8363010206517778 1103 1 0.3188577324066024 1103 2 0.30011395253381323 1103 3 0.1934337460087754 1103 4 0.2806273436697353 1103 5 0.25752896067626097 1103 6 0.10191479466912057 1103 7 0.19847454841942636 1103 8 0.22292090025634081 1103 9 0.24443048035297466 1103 10-11 0.2656027370218368 1103 12-13 0.18959816362827553 1103 14-15 0.23823494937263234 1103 16-17 0.15090646101703697 1103 18-19 0.28448330669969124 1103 20-21 0.262724757996736 1103 22-23 0.13961733407819565 1103 24-25 0.22530501102415457 1103 26-27 0.1694198570372194 1103 28-29 0.12474643829330745 1103 30-31 0.1302414325576322 1103 32-33 0.24773530491052753 1103 34-35 0.195740446186484 1103 36-37 0.20065727657547683 1103 38-39 0.27776965658943453 1103 40-41 0.19027577738987844 1103 42-43 0.24958254188909734 1103 44-45 0.1601667884330169 1103 46-47 0.1482470252179766 1103 48-49 0.29549592552420734 1103 50-51 0.1909451020985955 1103 52-53 0.25717104760382625 1103 54-55 0.199262407091922 1103 56-57 0.05710953901471072 1103 58-59 0.1167796313701146 1103 60-61 0.10743791442815365 1103 62-63 0.23949102722698257 1103 64-65 0.2736836170056378 1103 66-67 0.36615657055593687 1103 68-69 0.3390737379864994 1103 70-71 0.3628522729835666 1103 72-73 0.3631822616521596 1103 74-75 0.3843629222854297 1103 76-77 0.4205438633246743 1103 78-79 0.4117033680851314 1103 80-81 0.5692099405512927 1103 82-83 0.6179998175274832 1103 84-85 0.8432453090548329 1103 86-87 0.6463420489716718 1103 88-89 0.7026653445782607 1103 90-91 0.9678094710541565 1103 92-93 0.8441547823393911 1103 94-95 0.7046814737727125 1103 96-97 1.0227097494414252 1103 98-99 1.1023666423489615 1103 100-101 1.3308049604018954 1104 1 0.23464958294971439 1104 2 0.18597586759543816 1104 3 0.1294051286275959 1104 4 0.09239068246984061 1104 5 0.1513454301956827 1104 6 0.21778138421991855 1104 7 0.15182605151858297 1104 8 0.32754230624760794 1104 9 0.312788942366808 1104 10-11 0.2113515928283647 1104 12-13 0.3746656796258563 1104 14-15 0.395241665319503 1104 16-17 0.3699155221313575 1104 18-19 0.398629314501072 1104 20-21 0.41887778545473964 1104 22-23 0.41256458629725046 1104 24-25 0.31749016305069233 1104 26-27 0.08536884436328052 1104 28-29 0.24823284133992018 1104 30-31 0.32918848406416146 1104 32-33 0.3253859647529609 1104 34-35 0.2934630192154728 1104 36-37 0.48620386168946794 1104 38-39 0.4926812770526041 1104 40-41 0.5004142690352253 1104 42-43 0.34500550393066476 1104 44-45 0.47753489257112847 1104 46-47 0.4215838182014906 1104 48-49 0.33392247134079867 1104 50-51 0.317739300450107 1104 52-53 0.35263164324467056 1104 54-55 0.40972401950018167 1104 56-57 0.28143418096733086 1104 58-59 0.4092791890102703 1104 60-61 0.4387601610351908 1104 62-63 0.368495704357116 1104 64-65 0.4065539910439995 1104 66-67 0.5369252995438387 1104 68-69 0.4488925421095651 1104 70-71 0.33150216430829715 1104 72-73 0.3869541518624082 1104 74-75 0.54637820812912 1104 76-77 0.4301536359120952 1104 78-79 0.5179172695405576 1104 80-81 0.7854565634309374 1104 82-83 0.8321759322082869 1104 84-85 0.5604921933889564 1104 86-87 0.7090746180128207 1104 88-89 0.7503193848422534 1104 90-91 0.718871794935513 1104 92-93 0.7271413583685025 1104 94-95 0.6325477675366287 1104 96-97 0.6897223811260105 1104 98-99 0.9716849592159456 1104 100-101 0.950024901719889 1105 1 0.09803201879540069 1105 2 0.19498821390525478 1105 3 0.13251384734667582 1105 4 0.034513842403008255 1105 5 0.21032720452851095 1105 6 0.11104260515737963 1105 7 0.1899043430304772 1105 8 0.1457467689046581 1105 9 0.25147850310115416 1105 10-11 0.2040613685373529 1105 12-13 0.23391323429348887 1105 14-15 0.2712248226297902 1105 16-17 0.42107802368550296 1105 18-19 0.27024191390257357 1105 20-21 0.2708280902377638 1105 22-23 0.4733206423589351 1105 24-25 0.2610393055876443 1105 26-27 0.2330172291580226 1105 28-29 0.2356578803851832 1105 30-31 0.3589286733549457 1105 32-33 0.4010709814185205 1105 34-35 0.5355747678814637 1105 36-37 0.5801771371830071 1105 38-39 0.5493546116462937 1105 40-41 0.6904180415660832 1105 42-43 0.4087576563170714 1105 44-45 0.623789442175223 1105 46-47 0.5966390436251103 1105 48-49 0.6670260890531381 1105 50-51 0.45835506605246223 1105 52-53 0.4678792677424468 1105 54-55 0.6387543257819956 1105 56-57 0.4834519314489043 1105 58-59 0.5752997139626217 1105 60-61 0.7534005187698938 1105 62-63 0.6400466062730104 1105 64-65 0.6027935101357329 1105 66-67 0.5302624820508797 1105 68-69 0.5554077795575942 1105 70-71 0.6265792244252353 1105 72-73 0.6109311402757136 1105 74-75 0.4946374789321162 1105 76-77 0.5339940382699702 1105 78-79 0.5780282483718082 1105 80-81 0.4207535288854558 1105 82-83 0.5115210276626811 1105 84-85 0.615278198065873 1105 86-87 0.7404257972054786 1105 88-89 0.42692918252218703 1105 90-91 0.4205103762595286 1105 92-93 0.531772161295919 1105 94-95 0.7825554743999099 1105 96-97 0.8861718164386048 1105 98-99 0.7965092342172646 1105 100-101 1.2025311225636806 1106 1 0.11519754564355011 1106 2 0.1364339751103607 1106 3 0.0974110536829933 1106 4 0.19737722517903222 1106 5 0.11030200485610919 1106 6 0.09145685977207307 1106 7 0.17848399149504246 1106 8 0.2055977408420233 1106 9 0.19078404212914535 1106 10-11 0.05323257931161862 1106 12-13 -0.11021541703404125 1106 14-15 -0.09285881928286699 1106 16-17 -0.07370441914273584 1106 18-19 0.08946751396977248 1106 20-21 0.05473739941674438 1106 22-23 0.1375708821058197 1106 24-25 0.19330031119456947 1106 26-27 0.1172658589258404 1106 28-29 0.14544567814381537 1106 30-31 0.16869302141841303 1106 32-33 0.051543650273828234 1106 34-35 0.36217827203591213 1106 36-37 0.3130466098798621 1106 38-39 0.34205493154212974 1106 40-41 0.36336129330381794 1106 42-43 0.4069359299995057 1106 44-45 0.28486784815594746 1106 46-47 0.5409328928050741 1106 48-49 0.6032954175518626 1106 50-51 0.49659819389179916 1106 52-53 0.6193432987100422 1106 54-55 0.5165280397237169 1106 56-57 0.5378945986942298 1106 58-59 0.5306472944440728 1106 60-61 0.42012085140557076 1106 62-63 0.5971520389023866 1106 64-65 0.7830262921095681 1106 66-67 0.8355145137744628 1106 68-69 0.6988490398212086 1106 70-71 0.5197597380785588 1106 72-73 0.7645966426241806 1106 74-75 0.7763120671624684 1106 76-77 0.840960172710222 1106 78-79 0.8099352335810011 1106 80-81 0.7471006613727305 1106 82-83 0.5778355405740143 1106 84-85 0.8622472374683632 1106 86-87 0.7762530814507862 1106 88-89 0.95455169842948 1106 90-91 1.0180156086915098 1106 92-93 1.0954188338891733 1106 94-95 1.3080062685395895 1106 96-97 1.4587451229856185 1106 98-99 1.2785632175154795 1106 100-101 1.4184704403525465 1107 1 0.0217045513645715 1107 2 -0.010405519097151483 1107 3 -0.13705311934907982 1107 4 -0.19022531563683032 1107 5 -0.10990319879613963 1107 6 -0.22915031433726796 1107 7 -0.10349235744180874 1107 8 -0.1220325857894764 1107 9 -0.2602682977014936 1107 10-11 -0.10246952676256171 1107 12-13 -0.24713943659916282 1107 14-15 -0.13728823389813272 1107 16-17 0.069262230905899 1107 18-19 0.032210256363541134 1107 20-21 0.025549219370240905 1107 22-23 -0.12345062139617369 1107 24-25 -0.20922403189924665 1107 26-27 -0.11618292906176464 1107 28-29 -0.22847444025095598 1107 30-31 -0.19962156142828036 1107 32-33 -0.15364344204689928 1107 34-35 0.048940265781489245 1107 36-37 -0.1164198641105898 1107 38-39 -0.167018165722169 1107 40-41 -0.24433794005868492 1107 42-43 -0.2306391561992811 1107 44-45 -0.3142790540916707 1107 46-47 -0.21295651128156834 1107 48-49 -0.0455142722719728 1107 50-51 -0.14039903798554576 1107 52-53 -0.31888773494321754 1107 54-55 -0.19129818213747996 1107 56-57 -0.1883031198706675 1107 58-59 -0.19131718363929906 1107 60-61 -0.06523537878788233 1107 62-63 0.05147365710367069 1107 64-65 0.018586035099495746 1107 66-67 0.13935519681047737 1107 68-69 0.06988947393535838 1107 70-71 -0.157633258640832 1107 72-73 -0.16646830018305891 1107 74-75 0.030340487484810552 1107 76-77 0.05699532507899008 1107 78-79 0.20878196534851767 1107 80-81 0.22474475889153211 1107 82-83 0.06411128254593734 1107 84-85 0.1059012988342225 1107 86-87 0.019120110460292494 1107 88-89 0.10316005439471354 1107 90-91 0.1333376382762559 1107 92-93 0.21838196277422028 1107 94-95 0.22321159421235492 1107 96-97 0.17294560967317807 1107 98-99 0.19525196408583412 1107 100-101 0.2727182361390028 1108 1 0.11684002397040771 1108 2 -0.009898884436662314 1108 3 0.12294561197763798 1108 4 0.19231556986373732 1108 5 0.12310283908711028 1108 6 0.1940941172720656 1108 7 0.2825073320670981 1108 8 0.15922977751515077 1108 9 0.23588895769078988 1108 10-11 0.3154694300207552 1108 12-13 0.45638497117246146 1108 14-15 0.32518073785885804 1108 16-17 0.41707610651915417 1108 18-19 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-0.26976702965338006 2316 68-69 -0.27993945433496137 2316 70-71 -0.5126606852361562 2316 72-73 -0.5617261632562638 2316 74-75 -0.7260711298779476 2316 76-77 -0.7659801389888017 2316 78-79 -0.6027145448290234 2316 80-81 -0.482822613360959 2316 82-83 -0.6372261595030047 2316 84-85 -0.6864236435464512 2316 86-87 -0.5854530982370072 2316 88-89 -0.9587478036769959 2316 90-91 -0.9551710571895278 2316 92-93 -1.1065736871860743 2316 94-95 -1.188285834608127 2316 96-97 -1.503936536603355 2316 98-99 -2.005258188960081 2316 100-101 -1.9134457763300396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6806.0 3 1576.0 4 1554.0 5 1744.0 6 1885.0 7 2268.0 8 2814.0 9 3437.0 10 4419.0 11 5262.0 12 6316.0 13 7658.0 14 8599.0 15 10012.0 16 11725.0 17 13648.0 18 12907.0 19 10936.0 20 11288.0 21 12303.0 22 13386.0 23 14584.0 24 16950.0 25 18779.0 26 21399.0 27 25242.0 28 29316.0 29 34454.0 30 41494.0 31 50211.0 32 62429.0 33 79293.0 34 103862.0 35 141180.0 36 195988.0 37 243021.0 38 157427.0 39 14014.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27515554924902 18.57496678319423 14.469197593770291 23.68068007378646 2 15.193243913131932 19.178039995529943 41.30846530979487 24.32025078154326 3 19.429099057022757 23.137039721949222 29.511448391265354 27.922412829762667 4 12.804262595481092 15.924201103615657 36.75929845976631 34.51223784113694 5 14.105788939381023 36.6360346664355 34.41504151332159 14.84313488086189 6 33.146923013062555 35.3561501092125 17.193310412545554 14.303616465179392 7 29.348617215193133 31.938489460662755 20.112732584311477 18.60016073983263 8 26.196174303364806 35.79355150835046 19.198905307996633 18.8113688802881 9 25.913314554635704 16.97547032622262 20.233331043248256 36.87788407589342 10-11 25.185425349253826 26.32853872013387 27.20973408827556 21.276301842336743 12-13 25.734951554277885 23.372457753569826 27.951167112872554 22.94142357927973 14-15 22.6719849540226 25.074159979446325 25.813970630858996 26.439884435672077 16-17 22.45279764448061 28.49371863882012 26.472790072350342 22.580693644348923 18-19 22.30446558058201 27.109797984532797 28.774814496220863 21.81092193866433 20-21 22.890948103288935 26.226753781954887 28.925832144568158 21.956465970188024 22-23 22.696211358732153 25.973959061965214 28.7274674971296 22.602362082173038 24-25 22.454725692062787 26.138535285389242 28.90864703232471 22.498091990223262 26-27 22.29987945914402 26.53537528851627 28.643248084101668 22.52149716823804 28-29 22.329156973654687 26.514212183118573 28.825219472692993 22.331411370533743 30-31 22.374495730190556 26.39609331134163 28.64041380095169 22.588997157516122 32-33 22.15604413537214 26.360370638640205 28.694183040404937 22.789402185582713 34-35 22.68422308929147 26.54445069914467 28.40259767680632 22.368728534757544 36-37 22.583668891397252 26.29767503430116 28.48311059194768 22.63554548235391 38-39 22.326571759943274 26.509083442027453 28.584240866652884 22.580103931376385 40-41 22.633026383999947 26.367363936202143 28.438672682344112 22.560936997453794 42-43 22.416563590285428 26.275872112057414 28.481175933045495 22.826388364611663 44-45 22.502160335348453 26.58971161535833 28.30273678259423 22.60539126669899 46-47 22.610213474308868 26.85257612657454 27.865056526428262 22.672153872688327 48-49 22.66444145618652 26.688284223882704 27.915632843133398 22.731641476797385 50-51 22.68993865553165 26.923612306395277 27.72925271967156 22.657196318401514 52-53 22.832841052102587 26.840685576183805 27.597684297636622 22.728789074076992 54-55 22.643494824941037 26.875422190927615 27.696762439605234 22.784320544526114 56-57 22.635198344088813 27.178400747821623 27.71018263031876 22.4762182777708 58-59 22.697653553261812 26.85683601917911 27.745853535160215 22.699656892398867 60-61 22.706752985297264 26.810369682500735 27.915739890840175 22.56713744136183 62-63 22.576351054760543 26.96893583788953 27.888987110052344 22.565725997297587 64-65 22.52682723358161 26.766276011156954 27.838936075756237 22.8679606795052 66-67 22.379863535192595 27.092456802957383 27.651008103894693 22.87667155795533 68-69 22.467201648593434 26.941623048824553 27.86376922554032 22.72740607704169 70-71 22.47724735983515 26.88614378523797 27.710819667439395 22.925789187487478 72-73 22.615167116450646 26.817225057021925 27.534077217845216 23.033530608682216 74-75 22.544776132751643 27.14804698609628 27.428088985241274 22.879087895910807 76-77 22.585430999971738 26.973653848424217 27.43265351535431 23.008261636249742 78-79 22.666669051827153 27.011870943736447 27.52477585454337 22.796684149893025 80-81 22.52313583432993 27.131271019364732 27.461606624455925 22.883986521849405 82-83 22.557530886809893 27.184292014503992 27.427587351647443 22.830589747038672 84-85 22.7258483734367 27.002861000040777 27.336071995037788 22.935218631484737 86-87 22.5743093446384 26.986441213902317 27.497945042311738 22.94130439914754 88-89 22.66434674111033 26.850801218533267 27.384946907378023 23.09990513297838 90-91 22.540838014189067 27.048232877834582 27.494096236795905 22.916832871180443 92-93 22.767170466268606 26.63133916830856 27.54464585935678 23.056844506066057 94-95 22.58713625852832 26.792306767709146 27.481941709785247 23.13861526397729 96-97 22.633165146065366 26.690979593953845 27.505146673487165 23.170708586493628 98-99 22.64143451236417 26.56523722219738 27.421830702499665 23.371497562938785 100-101 22.939312687565653 26.008316818793563 27.805120204748107 23.24725028889268 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1721.0 1 964.0 2 193.0 3 174.0 4 157.0 5 140.5 6 208.0 7 428.0 8 708.5 9 875.0 10 853.5 11 807.5 12 870.5 13 965.0 14 1071.5 15 1174.5 16 1269.5 17 1406.0 18 1509.5 19 1594.5 20 1789.0 21 2124.0 22 2612.0 23 3348.0 24 4264.0 25 5517.5 26 7189.5 27 9049.0 28 10708.5 29 12628.5 30 15056.5 31 17421.5 32 19826.5 33 22034.5 34 24493.0 35 27035.0 36 30058.0 37 33502.0 38 35790.5 39 37423.5 40 39537.5 41 43327.5 42 46379.5 43 48580.0 44 51600.0 45 54575.0 46 56085.0 47 56450.0 48 56903.5 49 56501.5 50 56221.5 51 55102.0 52 52732.5 53 51120.0 54 49525.0 55 45996.0 56 40891.0 57 36391.0 58 32632.0 59 28243.5 60 23258.0 61 18717.0 62 14849.0 63 11410.5 64 8822.0 65 6774.0 66 5001.0 67 3905.5 68 3106.0 69 2274.5 70 1570.0 71 1036.5 72 722.0 73 536.0 74 388.0 75 281.5 76 198.5 77 128.0 78 89.0 79 61.5 80 39.0 81 30.5 82 21.0 83 11.5 84 13.5 85 13.0 86 7.5 87 3.0 88 4.5 89 5.5 90 3.0 91 2.0 92 3.0 93 2.5 94 0.5 95 0.0 96 1.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3434541243419629 2 0.3021739239115918 3 0.298745810381042 4 0.30210250487970536 5 0.31210116934380905 6 0.207900801821471 7 0.20697235440694708 8 0.20704377343883354 9 0.20418701216337534 10-11 0.21382858146804679 12-13 0.2035442408763972 14-15 0.20497262151412632 16-17 0.21125749632013435 18-19 0.20308001716913526 20-21 0.19486682850219292 22-23 0.1952239236616252 24-25 0.19915197041538024 26-27 0.1953667617253981 28-29 0.20836502552873293 30-31 0.21379287195210356 32-33 0.21647108564784562 34-35 0.21536409065360554 36-37 0.1885105346642984 38-39 0.18097582680027738 40-41 0.18686789693090994 42-43 0.19715223752255948 44-45 0.2024372458821572 46-47 0.20540113570544508 48-49 0.2046869453865805 50-51 0.20807934940118714 52-53 0.23489719587455105 54-55 0.19390267157172578 56-57 0.18051160309301542 58-59 0.18001166986981024 60-61 0.1728340571652215 62-63 0.18193998373074455 64-65 0.17808335600887595 66-67 0.1759764945682255 68-69 0.18797489192515 70-71 0.18190427421480132 72-73 0.17704778004652236 74-75 0.18136863147565288 76-77 0.18443964984677047 78-79 0.1897603677223114 80-81 0.1797974127741509 82-83 0.17876183681179728 84-85 0.1731197332927673 86-87 0.16769188686939673 88-89 0.17433385683483707 90-91 0.18293985017715492 92-93 0.1720484478144705 94-95 0.17790480842915982 96-97 0.1742267282870074 98-99 0.17851187020019468 100-101 0.15544352290086966 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1400187.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.17438900410899 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1104764627651 49.80819926900475 2 11.513109147983123 14.31641053626937 3 3.597906440864344 6.7109290396406704 4 1.5503969290743607 3.855799271161811 5 0.8247489938938881 2.5639132388553056 6 0.501197959424841 1.8697006138407417 7 0.36326642249545715 1.5810107505054491 8 0.2539212935492146 1.2629921025244337 9 0.1827183311743115 1.022436054055193 >10 1.02362893953225 11.42845017714735 >50 0.05522630284185607 2.351348324528676 >100 0.022711088002450558 2.4149352522546264 >500 2.3056279960684118E-4 0.10005900632457254 >1k 4.6112559921368236E-4 0.713816363887119 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3401 0.242896127445834 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2809 0.2006160605690526 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1964 0.14026697862499796 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1785 0.12748297191732247 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.141903188645516E-5 0.0 5 0.0 0.0 0.0 7.141903188645516E-5 0.0 6 0.0 0.0 0.0 7.141903188645516E-5 0.0 7 0.0 0.0 0.0 7.141903188645516E-5 7.141903188645516E-5 8 0.0 0.0 0.0 7.141903188645516E-5 7.141903188645516E-5 9 0.0 0.0 0.0 7.141903188645516E-5 7.141903188645516E-5 10-11 0.0 0.0 0.0 7.141903188645516E-5 7.141903188645516E-5 12-13 0.0 0.0 0.0 7.141903188645516E-5 7.141903188645516E-5 14-15 0.0 0.0 0.0 1.0712854782968275E-4 7.141903188645516E-5 16-17 0.0 0.0 0.0 1.4283806377291032E-4 7.141903188645516E-5 18-19 0.0 0.0 0.0 1.4283806377291032E-4 7.141903188645516E-5 20-21 0.0 0.0 0.0 1.4283806377291032E-4 7.141903188645516E-5 22-23 0.0 0.0 0.0 1.4283806377291032E-4 1.4283806377291032E-4 24-25 0.0 0.0 0.0 1.4283806377291032E-4 1.4283806377291032E-4 26-27 0.0 0.0 0.0 1.7854757971613792E-4 1.4283806377291032E-4 28-29 0.0 0.0 0.0 3.5709515943227585E-4 1.4283806377291032E-4 30-31 0.0 0.0 0.0 0.0010355759623536 1.4283806377291032E-4 32-33 0.0 0.0 0.0 0.0016069282174452412 1.4283806377291032E-4 34-35 0.0 0.0 0.0 0.001964023376877517 1.4283806377291032E-4 36-37 0.0 0.0 0.0 0.0026782136957420686 1.4283806377291032E-4 38-39 0.0 0.0 0.0 0.0037137896580956686 1.4283806377291032E-4 40-41 0.0 0.0 0.0 0.005285008359597682 1.4283806377291032E-4 42-43 0.0 0.0 0.0 0.007427579316191337 1.4283806377291032E-4 44-45 0.0 0.0 0.0 0.012748297191732247 1.4283806377291032E-4 46-47 0.0 0.0 0.0 0.02303263778338179 1.4283806377291032E-4 48-49 0.0 0.0 0.0 0.030960150322778317 1.4283806377291032E-4 50-51 0.0 0.0 0.0 0.041423038494144 1.4283806377291032E-4 52-53 0.0 0.0 0.0 0.05399278810616011 1.4283806377291032E-4 54-55 0.0 0.0 0.0 0.06916933238203182 1.4283806377291032E-4 56-57 0.0 0.0 0.0 0.08720263793336176 1.4283806377291032E-4 58-59 0.0 0.0 0.0 0.11034240426457323 1.4283806377291032E-4 60-61 0.0 0.0 0.0 0.13730308880171005 1.4283806377291032E-4 62-63 0.0 0.0 0.0 0.1709057433042872 1.4283806377291032E-4 64-65 0.0 0.0 0.0 0.20179447459517907 1.4283806377291032E-4 66-67 0.0 0.0 0.0 0.24950238789533113 1.4283806377291032E-4 68-69 0.0 0.0 0.0 0.3038522711609235 1.4283806377291032E-4 70-71 0.0 0.0 0.0 0.3452753096550675 1.4283806377291032E-4 72-73 0.0 0.0 0.0 0.41190926640513015 1.4283806377291032E-4 74-75 0.0 0.0 0.0 0.4753650762362456 1.4283806377291032E-4 76-77 0.0 0.0 0.0 0.5383923718760423 1.4283806377291032E-4 78-79 0.0 0.0 0.0 0.6204885490295224 1.4283806377291032E-4 80-81 0.0 0.0 0.0 0.7071198347077926 1.4283806377291032E-4 82-83 0.0 0.0 0.0 0.8006073474471624 1.4283806377291032E-4 84-85 0.0 0.0 0.0 0.9274475480775068 1.4283806377291032E-4 86-87 0.0 7.141903188645516E-5 0.0 1.093175411570026 1.4283806377291032E-4 88-89 0.0 7.141903188645516E-5 0.0 1.2924345105332358 1.4283806377291032E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2285 0.0 48.07987 1 GTATCAA 3780 0.0 45.043148 1 ATCAACG 4900 0.0 34.340874 3 CAACGCA 4970 0.0 33.864475 5 TCAACGC 4985 0.0 33.85674 4 TATCAAC 5090 0.0 33.24696 2 AACGCAG 5210 0.0 32.37141 6 ACGCAGA 5935 0.0 27.77863 7 CGCAGAG 5955 0.0 27.683353 8 GTGGTAT 1095 0.0 25.191347 1 GCAGAGT 6505 0.0 24.757559 9 GTACATG 4375 0.0 24.241768 1 ACATGGG 4275 0.0 22.794071 3 TACATGG 4430 0.0 22.64115 2 GAGTACT 3985 0.0 22.113 12-13 TGGTATC 1210 0.0 21.21427 2 GTACTTT 4250 0.0 21.068008 14-15 CAGAGTA 6235 0.0 20.914793 10-11 GGACCGA 370 0.0 20.5442 6 AGAGTAC 6130 0.0 20.536814 10-11 >>END_MODULE