FastQCFastQC Report
Thu 26 May 2016
SRR937751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400187
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36840.2631077134697008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32010.22861232106854298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21190.1513369285673985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.049.863881
GTATCAA38050.041.9738161
ATCAACG49800.031.6783733
CCGTGCG1805.456968E-1231.6670599
TCAACGC51050.030.9027024
TATCAAC51700.030.7899062
CAACGCA52000.030.3381335
AACGCAG53150.029.6679336
TGCACCG2450.029.0923865
GTGGTAT10650.028.1180171
ACCGTGC2600.025.5772428
ACGCAGA62350.025.1406487
CGCAGAG62600.024.9643698
TGGTATC11750.023.0510712
GCACCGT2903.6379788E-1222.9288646
AGGACCG4050.022.2921895
GCAGAGT70300.022.1624379
GTACATG44600.020.8888721
GTCTTAG4900.020.371221
AGAGTAC66300.020.31133810-11
Produced by FastQC (version 0.11.5)