##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937751_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1400187 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.161920514902654 33.0 31.0 34.0 31.0 34.0 2 32.446011139940595 34.0 31.0 34.0 31.0 34.0 3 32.54760756956035 34.0 31.0 34.0 31.0 34.0 4 36.173322563343326 37.0 35.0 37.0 35.0 37.0 5 36.07596128231443 37.0 35.0 37.0 35.0 37.0 6 36.110807342162154 37.0 35.0 37.0 35.0 37.0 7 36.05102889828287 37.0 35.0 37.0 35.0 37.0 8 36.01996376198322 37.0 35.0 37.0 35.0 37.0 9 37.731191619405124 39.0 37.0 39.0 35.0 39.0 10-11 37.640959029043984 39.0 37.0 39.0 35.0 39.0 12-13 37.53790386569794 39.0 37.0 39.0 35.0 39.0 14-15 38.7922984572775 40.0 38.0 41.0 35.0 41.0 16-17 38.738416368670755 40.0 38.0 41.0 35.0 41.0 18-19 38.67736880859485 40.0 38.0 41.0 34.0 41.0 20-21 38.55863716774974 40.0 38.0 41.0 34.0 41.0 22-23 38.50687943824646 40.0 38.0 41.0 34.0 41.0 24-25 38.54150052814374 40.0 38.0 41.0 34.0 41.0 26-27 38.41286378176629 40.0 38.0 41.0 34.0 41.0 28-29 38.260964428322794 40.0 38.0 41.0 34.0 41.0 30-31 38.34874306074832 40.0 38.0 41.0 34.0 41.0 32-33 38.315467148316614 40.0 38.0 41.0 34.0 41.0 34-35 38.19527391698395 40.0 38.0 41.0 34.0 41.0 36-37 38.06497560682966 40.0 38.0 41.0 33.0 41.0 38-39 37.94801658635596 40.0 37.5 41.0 33.0 41.0 40-41 37.78141169715188 40.0 37.0 41.0 33.0 41.0 42-43 37.593509652639256 40.0 37.0 41.0 33.0 41.0 44-45 37.34650585957447 40.0 37.0 41.0 32.0 41.0 46-47 37.06691427645022 40.0 36.5 41.0 31.5 41.0 48-49 36.81016321391357 39.5 36.0 41.0 31.0 41.0 50-51 36.571166922703895 39.0 36.0 41.0 30.5 41.0 52-53 36.329073902271624 39.0 35.5 40.5 30.0 41.0 54-55 36.10025732277189 39.0 35.0 40.0 30.0 41.0 56-57 35.82346536569758 38.5 35.0 40.0 29.0 41.0 58-59 35.548583510631076 38.0 35.0 40.0 28.0 41.0 60-61 35.21342613522337 38.0 34.0 40.0 28.0 41.0 62-63 34.817992525284126 37.5 34.0 40.0 27.5 41.0 64-65 34.40083574551113 37.0 34.0 39.5 26.5 41.0 66-67 33.96450759791371 36.0 33.0 39.0 26.0 40.5 68-69 33.503392046919444 36.0 33.0 39.0 26.0 40.0 70-71 33.098702173352564 35.0 32.0 38.0 25.0 40.0 72-73 32.60181247219121 35.0 32.0 37.5 24.5 39.5 74-75 32.11244462346815 35.0 31.0 37.0 23.0 39.0 76-77 30.890471058508613 33.5 29.5 35.5 21.5 38.0 78-79 31.49243351066679 34.0 31.0 36.0 23.0 38.0 80-81 31.30081517682995 34.0 31.0 36.0 22.0 37.0 82-83 30.923252037049338 34.0 31.0 35.0 20.5 37.0 84-85 30.543040679566374 34.0 31.0 35.0 19.5 36.5 86-87 30.156334118228493 34.0 30.0 35.0 18.0 36.0 88-89 29.722847733909827 34.0 30.0 35.0 14.0 36.0 90-91 29.16450124161987 34.0 29.0 35.0 7.0 35.0 92-93 28.70345318161074 33.5 29.0 35.0 2.0 35.0 94-95 28.2049640512303 33.0 29.0 35.0 2.0 35.0 96-97 27.608672984394225 33.0 27.0 35.0 2.0 35.0 98-99 26.662561500713835 32.5 25.5 34.5 2.0 35.0 100-101 23.832413813297798 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2451150156913826 1101 2 0.16390781879835714 1101 3 0.18971876462254755 1101 4 0.09321439750584659 1101 5 0.024771085044093866 1101 6 0.05805832727397586 1101 7 0.15160602238161403 1101 8 0.09887896500573845 1101 9 0.11309203027404635 1101 10-11 0.2328277445007032 1101 12-13 0.1612034757932932 1101 14-15 0.2732310973757137 1101 16-17 0.2981985770721849 1101 18-19 0.11992148228824817 1101 20-21 0.28156947127605036 1101 22-23 0.22653791251831734 1101 24-25 0.10865232083835963 1101 26-27 0.17028666383295388 1101 28-29 0.01490887782137662 1101 30-31 0.1511870326277318 1101 32-33 0.20992024698127665 1101 34-35 0.15575742844961837 1101 36-37 0.0976311411248929 1101 38-39 0.221407284318218 1101 40-41 0.22030554817012415 1101 42-43 0.348157916828427 1101 44-45 0.21348076311824116 1101 46-47 0.09867545586051563 1101 48-49 -0.029569530823479795 1101 50-51 0.16456453915377978 1101 52-53 0.18334645008671657 1101 54-55 0.061784814636553165 1101 56-57 -0.06737320068765484 1101 58-59 -0.06274182919609217 1101 60-61 0.13642714788080212 1101 62-63 -0.03408824865512372 1101 64-65 -0.07310208425951004 1101 66-67 -0.06576285073394672 1101 68-69 -0.01262000349437642 1101 70-71 -0.1262283035995111 1101 72-73 -0.07328128464186534 1101 74-75 -0.20331838552667847 1101 76-77 -0.02237731443424451 1101 78-79 -0.014914875984853637 1101 80-81 0.004908821311889966 1101 82-83 0.02049949069261814 1101 84-85 0.20546547701089324 1101 86-87 0.06482946595329508 1101 88-89 0.09956556174648412 1101 90-91 0.2176950695491051 1101 92-93 -0.1136305056985023 1101 94-95 -0.016057544347823693 1101 96-97 -0.051527931846052866 1101 98-99 -0.18106336323105765 1101 100-101 -0.46204318309641934 1102 1 0.22243197392871394 1102 2 0.16515027591164255 1102 3 0.053224046753399534 1102 4 0.07315758981997789 1102 5 -0.014587216725395535 1102 6 -0.011194669502678778 1102 7 0.0347962432409048 1102 8 0.11740054715139792 1102 9 0.14238961273996864 1102 10-11 0.12772990823783203 1102 12-13 0.1889846432110147 1102 14-15 0.268218101255016 1102 16-17 0.23618761971943059 1102 18-19 0.29221835429635235 1102 20-21 0.27629698682967074 1102 22-23 0.29396870317152946 1102 24-25 0.20708931364841732 1102 26-27 0.22945241368909564 1102 28-29 0.08283380563008791 1102 30-31 0.19548767059280436 1102 32-33 0.2605349400977488 1102 34-35 0.1866782599119503 1102 36-37 0.10896590952191332 1102 38-39 0.2669443503927269 1102 40-41 0.23122841502415525 1102 42-43 0.22937400066727065 1102 44-45 0.10691956704104655 1102 46-47 -0.14494115544381714 1102 48-49 -0.08921518938603867 1102 50-51 -0.03518142274003111 1102 52-53 0.13347187748279765 1102 54-55 -0.023717794475153653 1102 56-57 -0.1206835820052703 1102 58-59 -0.09312824975722123 1102 60-61 -0.1091329731044226 1102 62-63 -0.12074601490437686 1102 64-65 -0.10173599398169131 1102 66-67 -0.09048851900078603 1102 68-69 -0.22033558499662576 1102 70-71 -0.18151860978127132 1102 72-73 -0.07187821486603241 1102 74-75 -0.12575573332447476 1102 76-77 -0.2505587112435066 1102 78-79 -0.2171327294569103 1102 80-81 -0.060370579607699426 1102 82-83 -0.16761435233430788 1102 84-85 -0.23965708299095922 1102 86-87 -0.45244797095800493 1102 88-89 -0.22254747387625073 1102 90-91 -0.26637485531986016 1102 92-93 -0.4848632826432038 1102 94-95 -0.5218369474378584 1102 96-97 -0.6997464094405856 1102 98-99 -0.6949061972294324 1102 100-101 -0.5866373708473169 1103 1 0.16456206024476216 1103 2 0.13554402862667558 1103 3 0.11808473604063607 1103 4 0.004817490323567597 1103 5 -0.033801545245069065 1103 6 0.020783671365592227 1103 7 -0.029554552443201487 1103 8 0.026027706094637892 1103 9 0.08871138570625448 1103 10-11 0.03012024869486396 1103 12-13 0.13973313552833844 1103 14-15 0.0730756598603648 1103 16-17 -0.0825993892474699 1103 18-19 -0.09721045371966852 1103 20-21 -0.09117282015174766 1103 22-23 -0.19549604284959088 1103 24-25 -0.16584876646555102 1103 26-27 -0.04452508986070569 1103 28-29 -0.07418911040557674 1103 30-31 -0.07606517541006497 1103 32-33 0.047182906034443306 1103 34-35 -0.02649732595626375 1103 36-37 -0.13272388991993722 1103 38-39 -0.22742968458383928 1103 40-41 -0.19502225997124611 1103 42-43 0.05302769167638388 1103 44-45 0.14985807322414502 1103 46-47 -0.023675396674896376 1103 48-49 -0.02395945991400339 1103 50-51 -0.10014654527657996 1103 52-53 -0.11273332852625373 1103 54-55 -0.06123179223551034 1103 56-57 -0.052225155700867276 1103 58-59 -0.14243556323387452 1103 60-61 0.008011956163471723 1103 62-63 -0.0907259711304036 1103 64-65 -0.0908733151368537 1103 66-67 -0.31734046090348045 1103 68-69 -0.11206539788744152 1103 70-71 -0.1142397466078151 1103 72-73 -0.10071614835664633 1103 74-75 -0.1642809505965772 1103 76-77 -0.06227088857718144 1103 78-79 -0.04914432224225962 1103 80-81 0.21634580747425503 1103 82-83 0.03178530699563353 1103 84-85 -0.005191239378707024 1103 86-87 0.08605038543400667 1103 88-89 -0.11112553298314864 1103 90-91 0.008407085276839865 1103 92-93 0.024372923600189722 1103 94-95 -0.07222795849064667 1103 96-97 -0.1647950785766028 1103 98-99 -0.1885943535264083 1103 100-101 -0.24754747303907365 1104 1 0.24090544769125444 1104 2 0.17478861004861557 1104 3 0.16136205473251408 1104 4 0.06005172976833961 1104 5 0.0366287454447729 1104 6 0.03516630867541437 1104 7 0.11033547821828193 1104 8 0.07409967738217915 1104 9 0.06051391626869673 1104 10-11 0.12039863169133724 1104 12-13 0.08514130822562294 1104 14-15 0.2531161192807829 1104 16-17 0.144338477019879 1104 18-19 0.12009453160982275 1104 20-21 -0.027091383683860215 1104 22-23 0.042529870194350394 1104 24-25 -0.21093447863849946 1104 26-27 -0.115130343775256 1104 28-29 -0.06913762500081333 1104 30-31 -0.02361318135749002 1104 32-33 -0.020711408254960872 1104 34-35 0.04174955573690653 1104 36-37 0.15055741661298327 1104 38-39 0.14188665156738978 1104 40-41 0.18058350892940922 1104 42-43 0.2121597156245585 1104 44-45 -0.044789504624581866 1104 46-47 0.07339948230336546 1104 48-49 0.076404727688832 1104 50-51 0.3129033344279506 1104 52-53 0.29975798933636355 1104 54-55 0.04878640393985734 1104 56-57 0.2708130773950401 1104 58-59 0.17420165679195776 1104 60-61 0.11344413513565854 1104 62-63 0.08164713761617293 1104 64-65 0.0486093483184149 1104 66-67 0.35016933043471 1104 68-69 0.33875607095854576 1104 70-71 0.07659627389655554 1104 72-73 0.03787849420339029 1104 74-75 -0.025160097885240873 1104 76-77 -0.06922188038758392 1104 78-79 -0.2637245029363555 1104 80-81 0.06292022560598909 1104 82-83 -0.02721559356979597 1104 84-85 0.0696151126446587 1104 86-87 -0.10970441251978258 1104 88-89 -0.10556962530035108 1104 90-91 0.07950223622058772 1104 92-93 0.03587493996882429 1104 94-95 -0.04418894631320569 1104 96-97 0.07939614236299519 1104 98-99 0.20858801229330126 1104 100-101 -0.19242019224488516 1105 1 0.19874422181458584 1105 2 0.1745305791564462 1105 3 0.1122127050895827 1105 4 0.07978938984542339 1105 5 0.03017328941135844 1105 6 0.07573464087613502 1105 7 0.05169987404277521 1105 8 0.025204952283012005 1105 9 0.04927381809773124 1105 10-11 0.12481049157459978 1105 12-13 0.1586503069465408 1105 14-15 0.1924326610196161 1105 16-17 0.16547806236508933 1105 18-19 0.23516843233861096 1105 20-21 0.05222990991101284 1105 22-23 0.052023363217294616 1105 24-25 0.07811888010618873 1105 26-27 -0.05390527611665874 1105 28-29 0.04815764068585082 1105 30-31 0.09997595170980134 1105 32-33 0.11166865847540208 1105 34-35 0.01972091345628968 1105 36-37 0.021464050306207128 1105 38-39 0.1563131757805678 1105 40-41 0.1521745631772049 1105 42-43 0.14873712744697087 1105 44-45 0.20607400469773296 1105 46-47 0.11820999565710366 1105 48-49 0.08939249549168693 1105 50-51 0.24299420728769405 1105 52-53 0.2009468475113536 1105 54-55 -0.06568074097812371 1105 56-57 0.14587396587289447 1105 58-59 0.13681932483989812 1105 60-61 0.013469086637776684 1105 62-63 -0.022854947920642132 1105 64-65 0.035858960420483754 1105 66-67 0.13269442244870078 1105 68-69 -0.03079951979594142 1105 70-71 -0.04534248336535285 1105 72-73 0.05232971444254986 1105 74-75 -0.1396481066872468 1105 76-77 -0.21751193606434782 1105 78-79 -0.06218726047758594 1105 80-81 -0.06279808811529008 1105 82-83 -0.17738531487939824 1105 84-85 -0.011566260079689528 1105 86-87 0.012410836169198802 1105 88-89 -0.017990065775261854 1105 90-91 0.15215028038048573 1105 92-93 0.19239792685949197 1105 94-95 0.18277242910215463 1105 96-97 0.03680294892839342 1105 98-99 0.1716489314282974 1105 100-101 0.03189636652569661 1106 1 -0.0615281446446474 1106 2 0.10561472506255143 1106 3 0.08886275863888926 1106 4 0.09072324770527018 1106 5 -0.05953754434779768 1106 6 -0.12758296599497498 1106 7 0.030563648813703992 1106 8 -0.06944956720823114 1106 9 -0.07622405042997826 1106 10-11 -0.1982221840006133 1106 12-13 -0.30544365983189437 1106 14-15 -0.06937006054500472 1106 16-17 -0.08668053457315494 1106 18-19 0.045712395267095474 1106 20-21 0.10215063594156959 1106 22-23 0.12249319710945628 1106 24-25 -0.010854338241976791 1106 26-27 -0.03559351416956957 1106 28-29 0.06863062453705737 1106 30-31 0.15660171557487246 1106 32-33 0.055399014980764605 1106 34-35 -0.47142945158896765 1106 36-37 -0.09013462391102678 1106 38-39 -0.03226962264305655 1106 40-41 -0.09550409200027588 1106 42-43 -0.12644592017341694 1106 44-45 -0.23095656390486852 1106 46-47 -0.27067694881261417 1106 48-49 -0.03265179293256182 1106 50-51 0.11174241356101078 1106 52-53 0.21026372639192203 1106 54-55 0.07632641292887143 1106 56-57 0.13229064245609834 1106 58-59 0.14366611083168124 1106 60-61 0.04197446606784894 1106 62-63 -0.1257824848626612 1106 64-65 -0.05754170037092621 1106 66-67 -0.3874539406225779 1106 68-69 0.17587016849811477 1106 70-71 0.3261155626800516 1106 72-73 0.24344822990185122 1106 74-75 0.36912890421161393 1106 76-77 0.1550442207056122 1106 78-79 0.02311013065633105 1106 80-81 -0.11711913194172041 1106 82-83 -0.39280576441355564 1106 84-85 -0.5454414447022877 1106 86-87 -0.34877746838284196 1106 88-89 -0.3753100456155245 1106 90-91 -0.5061754113855201 1106 92-93 -0.2918584028082236 1106 94-95 -0.48628149819679223 1106 96-97 -0.18994947829005326 1106 98-99 -0.11159801136144765 1106 100-101 -0.31455712957885495 1107 1 0.1550504348273165 1107 2 0.03173042840625584 1107 3 -0.09645067721492495 1107 4 -0.059057812618185324 1107 5 -0.09116787795190362 1107 6 -0.0888606254322184 1107 7 0.04216169927442337 1107 8 -0.007477959640169729 1107 9 -0.13057896280181325 1107 10-11 -0.09273195784429333 1107 12-13 -0.08493601960417152 1107 14-15 -0.09419513541330815 1107 16-17 -0.15438013399721484 1107 18-19 0.02681359560038743 1107 20-21 0.019982017527631513 1107 22-23 -0.10748323549861283 1107 24-25 -0.10634977641766596 1107 26-27 -0.11154387970818647 1107 28-29 -0.183234982061812 1107 30-31 -0.13654735515170557 1107 32-33 -0.1829554337130972 1107 34-35 -0.08780870182516054 1107 36-37 -0.12586964066270667 1107 38-39 -0.1338007187010959 1107 40-41 -0.20425880286393294 1107 42-43 -0.09355093103324208 1107 44-45 -0.19505899838856777 1107 46-47 -0.35263987549222264 1107 48-49 -0.44666829010692055 1107 50-51 -0.4302670726628648 1107 52-53 -0.3339264408459215 1107 54-55 -0.4909936155193364 1107 56-57 -0.522403051174777 1107 58-59 -0.4094468069578667 1107 60-61 -0.4084616701349475 1107 62-63 -0.35756874326028765 1107 64-65 -0.21070926592209815 1107 66-67 -0.4613665634710955 1107 68-69 -0.26443259927592067 1107 70-71 -0.19287649552722286 1107 72-73 -0.35106581466004627 1107 74-75 -0.5398036951392413 1107 76-77 -0.36967582935766075 1107 78-79 -0.36923508631358715 1107 80-81 -0.28493264623729786 1107 82-83 -0.27084363958307733 1107 84-85 -0.27736192567231654 1107 86-87 -0.4695241816729663 1107 88-89 -0.35450293216782214 1107 90-91 -0.3818007665523915 1107 92-93 -0.6468078165867794 1107 94-95 -0.7438145221790542 1107 96-97 -0.5426689670589369 1107 98-99 -0.5911822351540543 1107 100-101 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0.08457459694336933 2316 72-73 0.24790265138997825 2316 74-75 0.32455101943839537 2316 76-77 0.23123760793883008 2316 78-79 0.3344382605884455 2316 80-81 0.1869247273012924 2316 82-83 0.22471268037413594 2316 84-85 0.3758988484782968 2316 86-87 0.2229999020827691 2316 88-89 0.11814957787090563 2316 90-91 0.4553830416512952 2316 92-93 0.4938364174997716 2316 94-95 0.5775145295977069 2316 96-97 0.5774589080382881 2316 98-99 0.6261829744088132 2316 100-101 0.9128535294671956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 8.0 5 29.0 6 59.0 7 131.0 8 298.0 9 569.0 10 953.0 11 1487.0 12 2323.0 13 3315.0 14 4395.0 15 5858.0 16 7517.0 17 10297.0 18 9349.0 19 6487.0 20 6094.0 21 6304.0 22 6615.0 23 7627.0 24 8913.0 25 10767.0 26 13095.0 27 16341.0 28 20765.0 29 25874.0 30 32738.0 31 41661.0 32 53572.0 33 70892.0 34 97544.0 35 140738.0 36 217048.0 37 319829.0 38 232577.0 39 18113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32615185046819 19.391289502501174 14.996896302206588 23.28566234482404 2 15.79353329233881 19.856704854423015 39.97058964266916 24.379172210569017 3 19.60195316768403 23.161691974000615 29.816303108084846 27.420051750230506 4 13.404352418641224 16.580713861791317 36.21730525994028 33.79762845962718 5 14.094174552651225 37.040942817234495 34.28658185884177 14.57830077127251 6 33.361543850928484 35.71080148580154 16.765546316313465 14.162108346956515 7 29.517057364480603 32.13699313020332 19.879130430435364 18.466819074880714 8 26.46189401844182 35.970409666708804 18.993105920852 18.574590393997372 9 26.091943433269986 16.850892059417777 19.980045522490926 37.07711898482131 10-11 25.34675725456671 26.521314652971352 26.941937041266627 21.189991051195303 12-13 25.782184341707413 23.570002431910957 27.850415692503606 22.79739753387802 14-15 22.58916130488285 25.392715401585647 25.609615001424807 26.408508292106696 16-17 22.448572940614362 28.664956895043304 26.44368216531078 22.44278799903156 18-19 22.40561439293466 27.277535072101085 28.672741569518926 21.64410896544533 20-21 22.870445161967652 26.438754252110613 28.91256667859364 21.778233907328094 22-23 22.729011143230053 26.165753984762645 28.70446782640058 22.400767045606717 24-25 22.609976538814514 26.321021864967843 28.798265943428902 22.27073565278874 26-27 22.45187646522125 26.7216003742396 28.57076538783614 22.255757772703006 28-29 22.424062143682804 26.68935193434818 28.762486390089236 22.124099531879775 30-31 22.497959201163845 26.57595021236449 28.597715876522205 22.328374709949454 32-33 22.33390968492066 26.624015220824077 28.55754267108608 22.484532423169192 34-35 22.745890370357674 26.720930847093992 28.36542547531151 22.16775330723682 36-37 22.75956711496393 26.528849360835373 28.433059298507985 22.27852422569271 38-39 22.543631672055234 26.714003201001006 28.470911385407803 22.27145374153595 40-41 22.731533541568393 26.59635668401324 28.334632570586436 22.337477203831934 42-43 22.586321302885437 26.580161439282417 28.323015704652256 22.510501553179893 44-45 22.688255211625304 26.79442103090516 28.1869850241432 22.330338733326336 46-47 22.73489183944716 27.1287692286816 27.763363036508693 22.372975895362547 48-49 22.900226898264304 26.977825104789577 27.741151717592004 22.380796279354115 50-51 22.953791172179145 27.137982283794948 27.60920505618178 22.299021487844122 52-53 23.124982591610976 27.157729646111555 27.361773820211155 22.35551394206631 54-55 22.843302849266347 27.17269503672729 27.522398825869438 22.461603288136924 56-57 22.87794416031573 27.42951477195546 27.495684504998263 22.19685656273055 58-59 22.91233242416906 27.05263654069064 27.535322067695244 22.499708967445063 60-61 22.87176641405755 27.11548528875072 27.751650315279313 22.261097981912418 62-63 22.743676380369195 27.21265088163224 27.701371316831253 22.342301421167317 64-65 22.693683058048673 27.051029612473194 27.585601066143305 22.669686263334825 66-67 22.67879218990035 27.272642868416863 27.486007226177644 22.562557715505143 68-69 22.730713826081804 27.175120180375906 27.598527910914754 22.495638082627533 70-71 22.71378751552471 27.17558440408317 27.45165467184026 22.65897340855186 72-73 22.875694460811307 27.055743268577697 27.399161683403715 22.66940058720728 74-75 22.81423838387301 27.444762735263218 27.13387568946148 22.60712319140229 76-77 22.746890236804084 27.322493352673604 27.262965589596245 22.66765082092606 78-79 22.849840771268408 27.32285044783304 27.296246858455337 22.531061922443214 80-81 22.683005912781653 27.471509162704695 27.299353586342395 22.546131338171257 82-83 22.785336731946416 27.49759137938792 27.185274168517026 22.53179772014864 84-85 22.930409627574612 27.310076193421377 27.099354264592613 22.660159914411402 86-87 22.729392573991902 27.305317075504913 27.278427809999666 22.68686254050352 88-89 22.792277031567927 27.33738422082193 27.115128193591286 22.755210554018856 90-91 22.66866760546982 27.439063806352742 27.224743832095427 22.667524756082013 92-93 22.874968351865125 27.069805875965024 27.33020440856287 22.725021363606984 94-95 22.723000570638067 27.24278971308832 27.26828630747179 22.765923408801825 96-97 22.754532073215934 27.160050764647863 27.29156891186677 22.793848250269427 98-99 22.709408051719322 27.029659906311576 27.166607269244587 23.094324772724516 100-101 22.737481168445708 27.038897472767914 27.318272375886753 22.905348982899625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 119.0 1 87.0 2 82.0 3 111.0 4 95.5 5 90.0 6 187.0 7 403.5 8 672.5 9 861.5 10 859.0 11 859.5 12 955.5 13 1091.0 14 1221.5 15 1297.0 16 1397.0 17 1471.0 18 1583.0 19 1756.5 20 1904.5 21 2226.0 22 2746.0 23 3424.5 24 4333.0 25 5531.0 26 7127.0 27 9017.5 28 10621.0 29 12542.5 30 14954.0 31 17362.5 32 19960.0 33 22061.5 34 24493.0 35 27130.5 36 30099.0 37 33481.5 38 35997.5 39 37558.5 40 39450.0 41 43367.5 42 46414.0 43 49037.0 44 52328.0 45 54972.0 46 56462.0 47 57047.0 48 57425.5 49 56702.5 50 56693.0 51 55894.5 52 53159.5 53 51140.5 54 49772.0 55 46361.0 56 40896.0 57 36092.0 58 32210.0 59 27959.0 60 22717.0 61 17891.5 62 14376.5 63 11182.5 64 8540.5 65 6482.5 66 4694.5 67 3694.5 68 2974.5 69 2129.5 70 1378.5 71 839.5 72 590.5 73 462.5 74 364.0 75 300.0 76 187.5 77 104.5 78 69.5 79 49.5 80 30.0 81 11.0 82 5.5 83 5.0 84 5.0 85 2.5 86 1.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017426243780295062 2 0.0 3 0.0 4 0.0 5 0.039423305601323254 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003820918205925352 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.78480802921324E-4 24-25 1.4283806377291035E-4 26-27 0.0010355759623536 28-29 8.927378985806896E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.6422370726195857E-4 42-43 0.011141368974287006 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.4283806377291035E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.713522550916414E-4 84-85 3.570951594322759E-5 86-87 0.0 88-89 0.0 90-91 0.012676878159845792 92-93 0.0018211853131046068 94-95 0.0 96-97 0.0 98-99 9.998664464103723E-4 100-101 0.019711652800661627 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1400187.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.92790531841082 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33161117623678 46.94249378008088 2 12.519285942419428 15.005091652228463 3 3.856691570308348 6.933703418032556 4 1.692797967919482 4.057833453787079 5 0.9470525841177833 2.837743879628341 6 0.5792890509208714 2.0829347637346824 7 0.3816780078037802 1.6011214459649223 8 0.2787915798872624 1.336591632244322 9 0.19992973792060134 1.0783233363996438 >10 1.1223842594660156 12.012711486744244 >50 0.06315863268896221 2.5797112994168416 >100 0.02637477112375845 2.661080921351525 >500 5.966994918039678E-4 0.2267117471106787 >1k 3.5801969508238065E-4 0.6439471832758836 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3684 0.2631077134697008 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3201 0.22861232106854298 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2119 0.1513369285673985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.141903188645516E-5 0.0 10-11 0.0 0.0 0.0 7.141903188645516E-5 0.0 12-13 0.0 0.0 0.0 7.141903188645516E-5 0.0 14-15 0.0 0.0 0.0 7.141903188645516E-5 7.141903188645516E-5 16-17 0.0 0.0 0.0 1.0712854782968275E-4 7.141903188645516E-5 18-19 0.0 0.0 0.0 1.4283806377291032E-4 7.141903188645516E-5 20-21 0.0 0.0 0.0 1.4283806377291032E-4 7.141903188645516E-5 22-23 0.0 0.0 0.0 1.4283806377291032E-4 7.141903188645516E-5 24-25 0.0 0.0 0.0 1.4283806377291032E-4 7.141903188645516E-5 26-27 0.0 0.0 0.0 1.7854757971613792E-4 7.141903188645516E-5 28-29 0.0 0.0 0.0 3.928046753755034E-4 7.141903188645516E-5 30-31 0.0 0.0 0.0 0.001106994994240055 7.141903188645516E-5 32-33 0.0 0.0 0.0 0.0016426377333884688 7.141903188645516E-5 34-35 0.0 0.0 0.0 0.001964023376877517 7.141903188645516E-5 36-37 0.0 0.0 0.0 0.0026782136957420686 7.141903188645516E-5 38-39 0.0 0.0 0.0 0.003678080142152441 7.141903188645516E-5 40-41 0.0 0.0 0.0 0.005249298843654455 7.141903188645516E-5 42-43 0.0 0.0 0.0 0.007606126895907475 7.141903188645516E-5 44-45 0.0 0.0 0.0 0.012962554287391613 7.141903188645516E-5 46-47 0.0 0.0 0.0 0.02321118536309793 7.141903188645516E-5 48-49 0.0 0.0 0.0 0.031138697902494454 7.141903188645516E-5 50-51 0.0 0.0 0.0 0.041780133653576274 7.141903188645516E-5 52-53 0.0 0.0 0.0 0.05442130229747884 7.141903188645516E-5 54-55 0.0 0.0 0.0 0.06981210366900993 7.141903188645516E-5 56-57 0.0 0.0 0.0 0.08798824728411277 7.141903188645516E-5 58-59 0.0 0.0 0.0 0.11141368974287005 7.141903188645516E-5 60-61 0.0 0.0 0.0 0.13837437428000687 7.141903188645516E-5 62-63 0.0 0.0 0.0 0.17186990023475435 7.141903188645516E-5 64-65 0.0 0.0 0.0 0.2026157934618733 7.141903188645516E-5 66-67 0.0 0.0 0.0 0.2503594162779686 7.141903188645516E-5 68-69 0.0 0.0 0.0 0.30417365680441255 7.141903188645516E-5 70-71 0.0 3.570951594322758E-5 0.0 0.3460609190058185 7.141903188645516E-5 72-73 0.0 1.0712854782968275E-4 0.0 0.4136590326863483 7.141903188645516E-5 74-75 0.0 1.4283806377291032E-4 0.0 0.47793616138415795 7.141903188645516E-5 76-77 0.0 1.4283806377291032E-4 0.0 0.5411420046036708 7.141903188645516E-5 78-79 0.0 1.4283806377291032E-4 0.0 0.6240237911079021 7.141903188645516E-5 80-81 0.0 1.4283806377291032E-4 0.0 0.7111907195253205 7.141903188645516E-5 82-83 0.0 1.4283806377291032E-4 0.0 0.8066779651575111 7.141903188645516E-5 84-85 0.0 1.4283806377291032E-4 0.0 0.9354464796487898 7.141903188645516E-5 86-87 0.0 1.4283806377291032E-4 0.0 1.1031383665181864 7.141903188645516E-5 88-89 0.0 1.4283806377291032E-4 0.0 1.306575478846754 7.141903188645516E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2345 0.0 49.86388 1 GTATCAA 3805 0.0 41.973816 1 ATCAACG 4980 0.0 31.678373 3 CCGTGCG 180 5.456968E-12 31.667059 9 TCAACGC 5105 0.0 30.902702 4 TATCAAC 5170 0.0 30.789906 2 CAACGCA 5200 0.0 30.338133 5 AACGCAG 5315 0.0 29.667933 6 TGCACCG 245 0.0 29.092386 5 GTGGTAT 1065 0.0 28.118017 1 ACCGTGC 260 0.0 25.577242 8 ACGCAGA 6235 0.0 25.140648 7 CGCAGAG 6260 0.0 24.964369 8 TGGTATC 1175 0.0 23.051071 2 GCACCGT 290 3.6379788E-12 22.928864 6 AGGACCG 405 0.0 22.292189 5 GCAGAGT 7030 0.0 22.162437 9 GTACATG 4460 0.0 20.888872 1 GTCTTAG 490 0.0 20.37122 1 AGAGTAC 6630 0.0 20.311338 10-11 >>END_MODULE