FastQCFastQC Report
Thu 26 May 2016
SRR937750_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937750_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322315
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31960.24169732628004673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26760.20237235454487018No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19240.14550239542015328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16360.12372241107451705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.049.478231
GTATCAA33900.043.20891
ATCAACG44100.032.7699623
TCAACGC44500.032.3685724
CAACGCA45400.031.7257025
TATCAAC46350.031.0766262
AACGCAG47300.030.6313976
GTGGTAT10350.026.6507741
ACGCAGA55100.026.1227637
CGCAGAG55050.025.8013238
GTACATG42000.024.6847881
TACATGG43200.024.0991272
GCAGAGT62550.022.4806529
ACATGGG44550.022.1950723
TGGTATC12050.022.0923522
ATAAGCG1100.002864265621.6081833
GAGTACT37200.020.55786912-13
GTATAGG3052.055458E-1020.2705571
GTCTAGA5000.019.9742971
CAGAGTA59850.019.92108310-11
Produced by FastQC (version 0.11.5)