##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937750_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1322315 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.457853083418097 31.0 31.0 34.0 30.0 34.0 2 31.598531363555583 33.0 31.0 34.0 30.0 34.0 3 31.64526304246719 33.0 31.0 34.0 30.0 34.0 4 35.24110518295565 37.0 35.0 37.0 33.0 37.0 5 35.206040164408634 37.0 35.0 37.0 33.0 37.0 6 35.276788057308586 37.0 35.0 37.0 33.0 37.0 7 35.194195785421776 37.0 35.0 37.0 32.0 37.0 8 35.17252470099787 37.0 35.0 37.0 32.0 37.0 9 36.75330008356556 39.0 37.0 39.0 33.0 39.0 10-11 36.62711570238559 39.0 37.0 39.0 32.0 39.0 12-13 36.48579347583594 39.0 37.0 39.0 32.0 39.0 14-15 37.610249827007934 40.0 37.0 41.0 32.0 41.0 16-17 37.5331649417877 40.0 37.0 41.0 32.0 41.0 18-19 37.44176614498058 40.0 37.0 41.0 32.0 41.0 20-21 37.24184781992188 40.0 37.0 41.0 31.0 41.0 22-23 37.08281687797537 39.5 36.5 41.0 31.0 41.0 24-25 36.92848867327376 39.0 36.0 41.0 31.0 41.0 26-27 37.03589613669965 39.0 37.0 41.0 31.0 41.0 28-29 36.97027334636603 39.0 36.0 41.0 31.0 41.0 30-31 36.77074335540321 39.0 36.0 41.0 30.0 41.0 32-33 36.53730956693375 39.0 36.0 41.0 30.0 41.0 34-35 36.27976200829605 39.0 35.0 40.0 30.0 41.0 36-37 36.30403232210177 39.0 35.0 41.0 30.0 41.0 38-39 36.26234104581737 39.0 35.0 41.0 30.0 41.0 40-41 36.06463361604459 39.0 35.0 41.0 29.0 41.0 42-43 35.82403852334731 39.0 35.0 40.0 28.0 41.0 44-45 35.61589182607776 39.0 35.0 40.0 27.0 41.0 46-47 35.30487251524788 38.5 35.0 40.0 26.0 41.0 48-49 35.00771261008156 38.0 34.0 40.0 24.5 41.0 50-51 34.13136393370718 37.5 33.5 39.5 23.5 40.5 52-53 34.23562199627169 38.0 33.5 39.5 24.0 40.5 54-55 34.51642951944128 38.0 34.0 40.0 24.0 41.0 56-57 34.22782241750264 38.0 34.0 40.0 23.0 41.0 58-59 33.97157598605476 38.0 33.0 40.0 22.5 41.0 60-61 33.634342044066656 37.5 33.0 40.0 21.5 41.0 62-63 33.23536903082851 37.0 32.5 40.0 19.5 41.0 64-65 32.84054328960951 36.0 32.0 39.0 18.5 41.0 66-67 32.394958841123334 36.0 31.5 39.0 16.5 40.5 68-69 31.889211723379074 35.0 31.0 39.0 12.5 40.0 70-71 31.42820054223086 35.0 31.0 38.0 8.0 40.0 72-73 30.92523490998741 35.0 30.0 37.5 7.0 39.5 74-75 30.40341484442058 34.5 29.5 37.0 4.0 39.0 76-77 29.922246590260265 34.0 29.0 36.0 2.0 39.0 78-79 29.360920809338168 34.0 29.0 36.0 2.0 38.0 80-81 28.81469128006564 34.0 28.5 35.0 2.0 37.0 82-83 28.27146292676102 34.0 27.0 35.0 2.0 37.0 84-85 27.77333842541301 33.0 26.0 35.0 2.0 36.0 86-87 27.211356219962717 33.0 25.5 35.0 2.0 36.0 88-89 26.68988667601895 32.5 25.0 35.0 2.0 35.0 90-91 26.14476089282811 32.0 24.0 35.0 2.0 35.0 92-93 25.540618914555154 32.0 21.5 34.0 2.0 35.0 94-95 24.83762605733127 31.5 18.5 34.0 2.0 35.0 96-97 23.987580871426246 31.0 6.0 34.0 2.0 35.0 98-99 22.979515470973254 31.0 2.0 34.0 2.0 35.0 100-101 20.474384696536 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34353176075785186 1101 2 0.4496082180531964 1101 3 0.44895241944976405 1101 4 0.37041425898006963 1101 5 0.3489378486954138 1101 6 0.31339165382770773 1101 7 0.3664452201196724 1101 8 0.3555236721187498 1101 9 0.5556599897770553 1101 10-11 0.4070145683891013 1101 12-13 0.41964886909206456 1101 14-15 0.7017334722366684 1101 16-17 0.6188614250425744 1101 18-19 0.7683881013192106 1101 20-21 0.7252782061104099 1101 22-23 0.7351675500653982 1101 24-25 0.6793369148666599 1101 26-27 0.7246365769186411 1101 28-29 0.5333842654739414 1101 30-31 0.5909056774477648 1101 32-33 0.7174541731545432 1101 34-35 0.7159852204347672 1101 36-37 0.7219092496813104 1101 38-39 0.6305052348103786 1101 40-41 0.7101355868948502 1101 42-43 0.5376905968796777 1101 44-45 0.6381776111439876 1101 46-47 0.6933957247026541 1101 48-49 0.7459040389925846 1101 50-51 0.5992498493501941 1101 52-53 0.5771291349962837 1101 54-55 0.8200828558625375 1101 56-57 1.027969560133947 1101 58-59 0.8734496205840898 1101 60-61 1.0308177153111515 1101 62-63 1.1504360898128425 1101 64-65 0.9227940048573515 1101 66-67 1.0841594798150709 1101 68-69 1.1374234804581285 1101 70-71 1.202619519961214 1101 72-73 1.228860201537497 1101 74-75 1.0721061528054072 1101 76-77 0.899322209687746 1101 78-79 1.059858282173046 1101 80-81 1.019638909269581 1101 82-83 1.1550169598931248 1101 84-85 1.2290124217126746 1101 86-87 1.4487487870477658 1101 88-89 1.3065773245695844 1101 90-91 1.4663971666351578 1101 92-93 1.7001170049063283 1101 94-95 1.8998867448545838 1101 96-97 1.9736544039976032 1101 98-99 2.388123563170911 1101 100-101 2.631903990900579 1102 1 0.24238803610406023 1102 2 0.2786396757379066 1102 3 0.35700493817190804 1102 4 0.21508554502898392 1102 5 0.25622545453678924 1102 6 0.20031211083161793 1102 7 0.20734053732955715 1102 8 0.1882273658022129 1102 9 0.24578149270704586 1102 10-11 0.2256257819091374 1102 12-13 0.38500350223527136 1102 14-15 0.5896146261757309 1102 16-17 0.5881179549547753 1102 18-19 0.4910887250713216 1102 20-21 0.5295105059936489 1102 22-23 0.6271818969713863 1102 24-25 0.663472565647723 1102 26-27 0.516277719232157 1102 28-29 0.453328212358997 1102 30-31 0.36412910069691407 1102 32-33 0.32343385740485786 1102 34-35 0.4629198449148859 1102 36-37 0.4972372346989218 1102 38-39 0.5599169060632434 1102 40-41 0.6394594123190274 1102 42-43 0.7601800168308657 1102 44-45 0.5416539696353411 1102 46-47 0.5287509138912654 1102 48-49 0.5784911500470074 1102 50-51 0.4540638241262869 1102 52-53 0.5355747871651246 1102 54-55 0.47608373058270814 1102 56-57 0.6623185407718779 1102 58-59 0.7280897727629707 1102 60-61 0.5724877112412727 1102 62-63 0.760313699079262 1102 64-65 0.792128941844318 1102 66-67 0.8897274568331142 1102 68-69 0.8141167204879771 1102 70-71 0.8542998284309071 1102 72-73 0.8032686556705251 1102 74-75 0.6208110576398731 1102 76-77 0.82800307840823 1102 78-79 0.9870247448785179 1102 80-81 1.080001142478764 1102 82-83 0.863619029036208 1102 84-85 1.0380299829349084 1102 86-87 1.155326196745289 1102 88-89 1.3125928947754595 1102 90-91 1.6466885811539278 1102 92-93 1.6471574181929185 1102 94-95 1.185462786867319 1102 96-97 1.4461366981568382 1102 98-99 1.7860462429546438 1102 100-101 2.0660633086562292 1103 1 0.06418282743549852 1103 2 0.07571712740822889 1103 3 0.05429920626847107 1103 4 0.00428843419354763 1103 5 0.11834293779260463 1103 6 0.1474598241995011 1103 7 0.20847246952401122 1103 8 0.16726147110173173 1103 9 0.051414980807507504 1103 10-11 0.11060802842287387 1103 12-13 0.030220877283177572 1103 14-15 0.2357338079975193 1103 16-17 0.09520666363068386 1103 18-19 0.06741180157666804 1103 20-21 0.11322029691780955 1103 22-23 0.1125709357904725 1103 24-25 0.07093165606921303 1103 26-27 -0.24787243471151044 1103 28-29 0.18634244140990575 1103 30-31 0.1870012974065247 1103 32-33 0.05109399821312621 1103 34-35 -0.0396193242566838 1103 36-37 0.06389259539715653 1103 38-39 0.3499586179649157 1103 40-41 0.3991134407824717 1103 42-43 0.47812125339222433 1103 44-45 0.14503636820099786 1103 46-47 0.3964755343289852 1103 48-49 0.3570078960112397 1103 50-51 0.29657190633582076 1103 52-53 0.33615975975260426 1103 54-55 0.34740801112918973 1103 56-57 0.3835191455966793 1103 58-59 0.3495218828706399 1103 60-61 0.4362109535971683 1103 62-63 0.2615422216340022 1103 64-65 0.22376789330922264 1103 66-67 0.34048195217962984 1103 68-69 0.4526742569619664 1103 70-71 0.39979143998097655 1103 72-73 0.4064360627890977 1103 74-75 0.2877597058422019 1103 76-77 0.1257328488481093 1103 78-79 0.23637454041408645 1103 80-81 0.5290993579607282 1103 82-83 0.15743666732622685 1103 84-85 0.5739486885706668 1103 86-87 0.6575624638884072 1103 88-89 0.8655097233533873 1103 90-91 0.8287834435705257 1103 92-93 0.9103795034675173 1103 94-95 0.8196205656629303 1103 96-97 0.8177478565245231 1103 98-99 1.2265450231118677 1103 100-101 1.5093060117441404 1104 1 0.1061073611639145 1104 2 0.154786831442145 1104 3 0.3393265794260216 1104 4 0.24465109939667684 1104 5 0.3134067542559009 1104 6 0.24625253800103053 1104 7 0.26620742594112556 1104 8 0.42053782851262156 1104 9 0.4991821411980766 1104 10-11 0.4322587909969542 1104 12-13 0.2976543617636551 1104 14-15 0.4460306360182713 1104 16-17 0.44905348388959254 1104 18-19 0.41262889804742997 1104 20-21 0.4412304977011985 1104 22-23 0.44465863543817363 1104 24-25 0.3085084115111911 1104 26-27 0.5469658703823228 1104 28-29 0.39072091527665975 1104 30-31 0.3320310980655492 1104 32-33 0.39009523966905135 1104 34-35 0.5161859443890151 1104 36-37 0.3657491063123288 1104 38-39 0.5341039549412585 1104 40-41 0.5199113235789952 1104 42-43 0.6804363510002176 1104 44-45 0.518084857103311 1104 46-47 0.3053595740784445 1104 48-49 0.16705502560068197 1104 50-51 0.14322307569229764 1104 52-53 0.1975586602527457 1104 54-55 0.18329395901794499 1104 56-57 0.4472060981720105 1104 58-59 0.4700937265800036 1104 60-61 0.33906955647331927 1104 62-63 0.3396989740240173 1104 64-65 0.5324574690813506 1104 66-67 0.42786255054995337 1104 68-69 0.32299180669825844 1104 70-71 0.6317018264770375 1104 72-73 0.44169222356357807 1104 74-75 0.2562604554212342 1104 76-77 0.42721028253927784 1104 78-79 0.46345630983399033 1104 80-81 0.717279756501938 1104 82-83 0.7799777776355015 1104 84-85 0.7970829096131453 1104 86-87 0.8071823092407335 1104 88-89 0.6883586250189069 1104 90-91 0.8988568798879726 1104 92-93 1.0263661398959592 1104 94-95 1.262118464686786 1104 96-97 1.4160196689666087 1104 98-99 1.1620335116096001 1104 100-101 0.936974163496405 1105 1 0.005160024221293469 1105 2 0.21603875234449532 1105 3 0.25036044098212784 1105 4 0.12199976476703256 1105 5 0.24149069616628083 1105 6 0.1450020965374179 1105 7 0.31485976099611435 1105 8 0.29039322690788083 1105 9 0.3119785645647255 1105 10-11 0.16506832182994913 1105 12-13 0.24307525080710235 1105 14-15 0.27406618043593767 1105 16-17 0.3317461706360305 1105 18-19 0.32915201856259557 1105 20-21 0.6321322201197077 1105 22-23 0.3750821653699745 1105 24-25 0.4159010044846667 1105 26-27 0.43723504253261325 1105 28-29 0.4801326895341589 1105 30-31 0.3217740320541651 1105 32-33 0.4077456654361882 1105 34-35 0.32759381527370834 1105 36-37 0.3932281021162325 1105 38-39 0.3417728601611074 1105 40-41 0.3664484220839057 1105 42-43 0.4684194745734871 1105 44-45 0.2845992047272077 1105 46-47 0.24614030276516274 1105 48-49 0.3093846995783949 1105 50-51 0.17517353975344463 1105 52-53 0.26079722380794124 1105 54-55 0.2782964781232238 1105 56-57 0.37418564808886146 1105 58-59 0.5735980568701109 1105 60-61 0.6303369889299546 1105 62-63 0.570640036883475 1105 64-65 0.6464894944194484 1105 66-67 0.4849400185627317 1105 68-69 0.7021642862511079 1105 70-71 0.717250596761243 1105 72-73 0.46541285862167214 1105 74-75 0.4418979920294497 1105 76-77 0.6018805774392284 1105 78-79 0.6672496719351848 1105 80-81 0.46442653712147575 1105 82-83 0.6315902981976684 1105 84-85 0.5096559287100817 1105 86-87 0.5070276447463193 1105 88-89 0.36976307244628615 1105 90-91 0.5629985776431496 1105 92-93 0.666226774963075 1105 94-95 0.844908622991241 1105 96-97 0.8930130435167456 1105 98-99 0.9630927536588594 1105 100-101 1.0469136012284963 1106 1 0.0393309512011335 1106 2 0.1918866721492023 1106 3 0.11134268122669155 1106 4 0.12778504127310697 1106 5 0.1610709471104741 1106 6 0.2054718007022629 1106 7 0.10829269538110964 1106 8 0.12295410795795902 1106 9 0.13765276376188496 1106 10-11 -0.04196736277579305 1106 12-13 -0.31794909790293246 1106 14-15 -0.15608361996622477 1106 16-17 -0.02750976164910668 1106 18-19 -0.13801497211046865 1106 20-21 0.05557388227254023 1106 22-23 -0.17114462859905188 1106 24-25 -0.02590583823372583 1106 26-27 -0.06736558556987404 1106 28-29 0.2515627913364682 1106 30-31 0.24848373777720667 1106 32-33 0.26766012218132573 1106 34-35 0.08881632419625163 1106 36-37 0.014188842928419376 1106 38-39 0.28180586058405055 1106 40-41 0.033543670690804106 1106 42-43 0.26472649722673225 1106 44-45 0.08957857714412398 1106 46-47 0.4911702980905659 1106 48-49 0.7742579846322926 1106 50-51 0.6130060482307869 1106 52-53 0.4425968134822824 1106 54-55 0.4284282293901924 1106 56-57 0.6192796408617838 1106 58-59 0.3829494288269828 1106 60-61 0.18597674080535853 1106 62-63 0.4347140958427502 1106 64-65 0.7177527391085832 1106 66-67 0.8269783187163284 1106 68-69 0.7697952120437499 1106 70-71 0.6227778429789019 1106 72-73 0.48059764429515184 1106 74-75 0.5244222368595679 1106 76-77 0.6974779240494016 1106 78-79 0.8003826808911221 1106 80-81 0.7985168644174045 1106 82-83 0.8196954914514123 1106 84-85 0.7234714008813938 1106 86-87 0.49993250743932904 1106 88-89 0.8438814380442246 1106 90-91 1.1488288935122917 1106 92-93 1.214101271330989 1106 94-95 1.0236483926746303 1106 96-97 0.9858176899169386 1106 98-99 1.252311595249406 1106 100-101 1.00211979358442 1107 1 0.33786574973593275 1107 2 0.44331117357370076 1107 3 0.45726758965159675 1107 4 0.3947518707399382 1107 5 0.42277220881173605 1107 6 0.2795713123017691 1107 7 0.42147950856791994 1107 8 0.1917626142664659 1107 9 0.4677046563137779 1107 10-11 0.46972118016275033 1107 12-13 0.4146261971723675 1107 14-15 0.6126002424676358 1107 16-17 0.6849826070932679 1107 18-19 0.6731114015159037 1107 20-21 0.8588527793014364 1107 22-23 0.7147807885763626 1107 24-25 0.7113753990475118 1107 26-27 0.8142354522811672 1107 28-29 0.7571717656954462 1107 30-31 0.7890184345619105 1107 32-33 0.8296895279607313 1107 34-35 0.8515732432031697 1107 36-37 0.8691440728836497 1107 38-39 0.8180543343325226 1107 40-41 0.8889949323920661 1107 42-43 0.9525953413455781 1107 44-45 0.8980848856504338 1107 46-47 0.8488982183184888 1107 48-49 0.6691246919990022 1107 50-51 0.7794692146939539 1107 52-53 0.5766780100634747 1107 54-55 0.7172586119705713 1107 56-57 0.7632305973127416 1107 58-59 0.7838829860105463 1107 60-61 1.0095929394215517 1107 62-63 0.7638756750043285 1107 64-65 0.6562320838379279 1107 66-67 0.8526066255946319 1107 68-69 1.1117009914495277 1107 70-71 1.0363665690676278 1107 72-73 1.0270338469813574 1107 74-75 1.0409566283939604 1107 76-77 0.8803737319452125 1107 78-79 0.9699365066949461 1107 80-81 1.2270628554633944 1107 82-83 1.2418566636125803 1107 84-85 1.397057074467071 1107 86-87 1.437114538371361 1107 88-89 1.5691180070307915 1107 90-91 1.818115625299022 1107 92-93 1.569298157777876 1107 94-95 1.7796770862033213 1107 96-97 1.4820020611013085 1107 98-99 1.6069361373739497 1107 100-101 1.5505909983056263 1108 1 -0.039552307422916044 1108 2 0.07135985926222332 1108 3 0.06956513748802706 1108 4 0.18547453799739344 1108 5 0.15141246098541217 1108 6 -0.05688989967103453 1108 7 0.029282006206706512 1108 8 0.017354472259420106 1108 9 0.14670501778343947 1108 10-11 0.20786069968016818 1108 12-13 0.15684682581864706 1108 14-15 0.19440801834089427 1108 16-17 -0.08022243948968111 1108 18-19 0.08411990188319862 1108 20-21 0.06318176722523816 1108 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78-79 -0.3694740944990933 2316 80-81 -0.08229348765090805 2316 82-83 -0.08518481932062016 2316 84-85 -0.044613221502590505 2316 86-87 0.2937516262186399 2316 88-89 -0.0481134699140604 2316 90-91 0.05417728715686465 2316 92-93 0.1498632402537865 2316 94-95 0.1359961563758958 2316 96-97 0.30870737984484364 2316 98-99 0.26255429840612265 2316 100-101 0.6000361686106679 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6420.0 3 1468.0 4 1474.0 5 1620.0 6 1894.0 7 2146.0 8 2664.0 9 3305.0 10 3991.0 11 5067.0 12 5887.0 13 7068.0 14 7870.0 15 9198.0 16 10680.0 17 13135.0 18 11927.0 19 10066.0 20 10580.0 21 11034.0 22 12091.0 23 13786.0 24 14922.0 25 17046.0 26 19669.0 27 22813.0 28 26820.0 29 31769.0 30 38074.0 31 46454.0 32 58236.0 33 74189.0 34 97109.0 35 132421.0 36 184076.0 37 230773.0 38 157590.0 39 16980.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.249810658665005 18.54209448701779 14.509161995741149 23.698932858576065 2 15.222189690665347 19.22984151737969 41.23362192026869 24.31434687168627 3 19.35172172134971 23.056905808747516 29.61841579337372 27.972956676529055 4 12.823651842547587 15.914614166348095 36.796946583413735 34.46478740769058 5 14.072023211271878 36.614416020328825 34.398725655661536 14.914835112737753 6 33.040606555351665 35.44002625596994 17.217898021630855 14.301469167047545 7 29.361070332057288 31.851670518045143 20.145046273383958 18.642212876513614 8 26.09612042602632 35.79516904404793 19.347924777691293 18.760785752234455 9 25.819640445145826 17.030723915294345 20.27836015820535 36.87127548135447 10-11 25.12687498507743 26.233589439332132 27.342350417130767 21.297185158459662 12-13 25.70942836061119 23.413798186916406 27.967047522102668 22.90972593036974 14-15 22.63302324294641 25.170794783629624 25.765748421661783 26.430433551762178 16-17 22.38782782995186 28.464288488463076 26.558967237371334 22.58891644421373 18-19 22.268945775667827 27.09090743734383 28.823051498981023 21.81709528800732 20-21 22.883214126442788 26.29433600240068 28.871546413392597 21.950903457763932 22-23 22.67766178638865 25.98362478964482 28.749267238324975 22.589446185641545 24-25 22.413565744600227 26.144562334217508 28.9240621447518 22.517809776430468 26-27 22.312171846390765 26.52095029647504 28.649780523590685 22.51709733354351 28-29 22.291211508544816 26.52577845065912 28.885659226864384 22.297350813931676 30-31 22.337649443155136 26.429363997823252 28.650225330036882 22.582761228984737 32-33 22.141110830240493 26.40390567648783 28.707419519514172 22.747563973757504 34-35 22.70514631287463 26.484796095746375 28.475122623744536 22.334934967634457 36-37 22.581226958066996 26.305333793066865 28.523004990129625 22.590434258736515 38-39 22.34473007439378 26.460521589956766 28.628420575661462 22.566327759988 40-41 22.626102360922 26.352781256890346 28.469799156072106 22.551317226115543 42-43 22.367305274596863 26.292391039937435 28.519789116156623 22.82051456930908 44-45 22.46181232728729 26.525868115220995 28.371092520016962 22.64122703747475 46-47 22.56039661434908 26.82201537017011 27.948102751731817 22.669485263749 48-49 22.612980010376248 26.721450825192072 27.932114029572684 22.733455134859 50-51 22.667569597554053 26.91011947033781 27.798435206119116 22.623875725989024 52-53 22.777513926724037 26.882396173557478 27.671738292436 22.668351607282492 54-55 22.620558784367013 26.803143209429326 27.79329661860205 22.78300138760162 56-57 22.663633367303255 27.12258343151777 27.699301785504566 22.514481415674403 58-59 22.679287128772888 26.80738148521532 27.798393876469348 22.714937509542445 60-61 22.65436394278612 26.74884055868347 28.019898107808437 22.57689739072197 62-63 22.522164845151995 26.872585146608287 28.036109291637956 22.569140716601765 64-65 22.485867701532523 26.75324980357601 27.84401852030043 22.916863974591035 66-67 22.306280150046558 27.027928025536102 27.72629913695196 22.939492687465386 68-69 22.374158499486672 26.91945462053394 27.861745774976036 22.84464110500335 70-71 22.43009454838061 26.8734176112223 27.71066536192402 22.98582247847307 72-73 22.554972187632238 26.773935816563764 27.672311474273325 22.99878052153067 74-75 22.523395682668195 27.07666403083581 27.523530189609318 22.876410096886676 76-77 22.60077613507766 26.939797816925925 27.483577313639117 22.975848734357292 78-79 22.617743639833492 26.95698232561946 27.564327220607137 22.86094681393991 80-81 22.41735826811329 27.105396953847016 27.578360660209444 22.898884117830246 82-83 22.582438852185426 27.067988142774567 27.48907418644449 22.860498818595513 84-85 22.656532603454526 26.937285446273645 27.425718910045287 22.98046304022654 86-87 22.539923286077173 26.95904221993092 27.52909348628642 22.971941007705485 88-89 22.627687731620995 26.86240988492014 27.416955480659848 23.092946902799017 90-91 22.52185528610763 26.965763461325725 27.56830866073959 22.944072591827055 92-93 22.647933017696424 26.64033075959696 27.674977327329714 23.036758895376895 94-95 22.47027394790156 26.810497270218793 27.581965412164383 23.137263369715264 96-97 22.527172368854725 26.76420558941973 27.580191163155444 23.128430878570104 98-99 22.546824000106085 26.589818538647915 27.462363676661976 23.400993784584028 100-101 22.88329233396392 26.104388157331513 27.778859477597074 23.233460031107498 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1576.0 1 915.5 2 230.0 3 191.0 4 164.5 5 163.5 6 238.5 7 412.0 8 654.5 9 800.0 10 815.5 11 810.0 12 851.5 13 956.5 14 1085.5 15 1166.5 16 1230.0 17 1336.5 18 1417.0 19 1519.0 20 1733.0 21 2075.5 22 2561.5 23 3258.5 24 4073.5 25 5181.0 26 6810.0 27 8607.5 28 10046.0 29 11810.5 30 14205.5 31 16423.5 32 18738.5 33 20875.5 34 23257.5 35 25617.0 36 28351.5 37 31655.5 38 34120.5 39 35809.5 40 37503.0 41 40915.5 42 43926.0 43 46086.5 44 48881.0 45 51677.0 46 53078.5 47 53177.0 48 53587.0 49 53366.0 50 52889.0 51 51925.0 52 49513.0 53 47721.5 54 46533.5 55 43444.0 56 38642.5 57 34355.0 58 30631.5 59 26401.0 60 21733.0 61 17528.5 62 13959.5 63 10705.5 64 8361.0 65 6455.5 66 4760.5 67 3713.0 68 2942.0 69 2145.0 70 1456.5 71 948.5 72 662.5 73 484.0 74 370.0 75 276.0 76 184.0 77 129.0 78 81.5 79 53.5 80 37.5 81 27.5 82 21.0 83 13.0 84 12.0 85 8.0 86 3.5 87 5.5 88 6.0 89 4.5 90 4.0 91 4.5 92 3.0 93 2.0 94 2.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3470428755629332 2 0.29773541100267337 3 0.2902485413838609 4 0.2966766617636494 5 0.3017435331218356 6 0.22657233715113267 7 0.2173460937824951 8 0.2167410942173385 9 0.22816046100966864 10-11 0.2272151491891115 12-13 0.2238876515807504 14-15 0.224492651145907 16-17 0.20777953815845693 18-19 0.21568234497831457 20-21 0.20346891625671643 22-23 0.2133001591905106 24-25 0.21288422198946544 26-27 0.21258172220688715 28-29 0.222904527287371 30-31 0.2205979664452116 32-33 0.2272151491891115 34-35 0.22184577804834704 36-37 0.20494360269678555 38-39 0.195187984708636 40-41 0.1916336122633412 42-43 0.2061536018270987 44-45 0.20770391321281237 46-47 0.20766610073999012 48-49 0.22350952685252756 50-51 0.2199173419344105 52-53 0.24037388973126678 54-55 0.2096323493267489 56-57 0.1905748630243172 58-59 0.1931082987034103 60-61 0.18078143256334533 62-63 0.18819267723651323 64-65 0.1864911159595104 66-67 0.18588611639435385 68-69 0.1890623641114258 70-71 0.19015892582327207 72-73 0.18819267723651323 74-75 0.20271266680027072 76-77 0.19439392277936798 78-79 0.19938516919190963 80-81 0.19299486128494345 82-83 0.17212237628704205 84-85 0.17231143865115348 86-87 0.17741612248216196 88-89 0.18191580674801391 90-91 0.1888354892744921 92-93 0.18482736715532985 94-95 0.1836929929706613 96-97 0.1890623641114258 98-99 0.19757017049643996 100-101 0.1796848708514991 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1322315.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.1530380333716 #Duplication Level Percentage of deduplicated Percentage of total 1 80.66767453865421 50.94408718203272 2 11.24365166971774 14.201415230633332 3 3.4172601880174533 6.474310878713786 4 1.5389035569905047 3.8874573945724875 5 0.83163379152223 2.626010023292021 6 0.4918959227416416 1.8638833150417988 7 0.34374572881935483 1.5196010960156432 8 0.2391688439933833 1.2083391280889326 9 0.18614504336745397 1.0580062503157583 >10 0.965265178625699 10.902282109016978 >50 0.053665179280338086 2.333760904719394 >100 0.02027069934365701 2.170345606414077 >500 2.3988630875112574E-4 0.09604814180275309 >1k 4.797726175022515E-4 0.7144527393403526 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3196 0.24169732628004673 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2676 0.20237235454487018 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1924 0.14550239542015328 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1636 0.12372241107451705 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.562494564457032E-5 0.0 4 0.0 0.0 0.0 7.562494564457032E-5 0.0 5 0.0 0.0 0.0 7.562494564457032E-5 0.0 6 0.0 0.0 0.0 7.562494564457032E-5 7.562494564457032E-5 7 0.0 0.0 0.0 7.562494564457032E-5 7.562494564457032E-5 8 0.0 0.0 0.0 7.562494564457032E-5 7.562494564457032E-5 9 0.0 0.0 0.0 7.562494564457032E-5 7.562494564457032E-5 10-11 0.0 0.0 0.0 7.562494564457032E-5 7.562494564457032E-5 12-13 0.0 0.0 0.0 7.562494564457032E-5 1.5124989128914064E-4 14-15 0.0 0.0 0.0 7.562494564457032E-5 1.5124989128914064E-4 16-17 0.0 0.0 0.0 7.562494564457032E-5 1.5124989128914064E-4 18-19 0.0 0.0 0.0 7.562494564457032E-5 1.5124989128914064E-4 20-21 0.0 0.0 0.0 7.562494564457032E-5 1.5124989128914064E-4 22-23 0.0 0.0 0.0 1.5124989128914064E-4 1.5124989128914064E-4 24-25 0.0 0.0 0.0 1.5124989128914064E-4 1.5124989128914064E-4 26-27 0.0 0.0 0.0 1.8906236411142578E-4 1.5124989128914064E-4 28-29 0.0 0.0 0.0 4.1593720104513677E-4 1.5124989128914064E-4 30-31 0.0 0.0 0.0 0.0012478116031354103 1.5124989128914064E-4 32-33 0.0 0.0 0.0 0.0020040610595811136 1.5124989128914064E-4 34-35 0.0 0.0 0.0 0.002873747934493672 1.5124989128914064E-4 36-37 0.0 0.0 0.0 0.003932497173517657 1.5124989128914064E-4 38-39 0.0 0.0 0.0 0.005407183613586778 1.5124989128914064E-4 40-41 0.0 0.0 0.0 0.007524682091634747 1.5124989128914064E-4 42-43 0.0 0.0 0.0 0.010398430026128419 1.5124989128914064E-4 44-45 0.0 0.0 0.0 0.016750925460272327 1.5124989128914064E-4 46-47 0.0 0.0 0.0 0.02919122901880414 1.5124989128914064E-4 48-49 0.0 0.0 0.0 0.03871997217002 1.5124989128914064E-4 50-51 0.0 0.0 0.0 0.051273713147018674 1.5124989128914064E-4 52-53 0.0 0.0 0.0 0.06738182656931216 1.5124989128914064E-4 54-55 0.0 0.0 0.0 0.08795181178463528 1.8906236411142578E-4 56-57 0.0 0.0 0.0 0.10814367227173555 2.2687483693371096E-4 58-59 0.0 0.0 0.0 0.13517959033966945 2.2687483693371096E-4 60-61 0.0 0.0 0.0 0.1666395677278107 2.2687483693371096E-4 62-63 0.0 0.0 0.0 0.20244797949051474 2.2687483693371096E-4 64-65 0.0 0.0 0.0 0.2384076411445079 2.2687483693371096E-4 66-67 0.0 0.0 0.0 0.29278197706295395 2.2687483693371096E-4 68-69 0.0 0.0 0.0 0.3567984935510828 2.2687483693371096E-4 70-71 0.0 0.0 0.0 0.40421533447022834 3.024997825782813E-4 72-73 0.0 0.0 0.0 0.48252496568518094 3.024997825782813E-4 74-75 0.0 0.0 0.0 0.5543308515747004 3.024997825782813E-4 76-77 0.0 7.562494564457032E-5 0.0 0.6234142394210154 3.024997825782813E-4 78-79 0.0 7.562494564457032E-5 0.0 0.7101560520753376 3.024997825782813E-4 80-81 0.0 7.562494564457032E-5 0.0 0.8024562982345356 3.024997825782813E-4 82-83 0.0 7.562494564457032E-5 0.0 0.9116587197452952 3.7812472822285157E-4 84-85 0.0 7.562494564457032E-5 0.0 1.0487667461989012 3.7812472822285157E-4 86-87 0.0 7.562494564457032E-5 0.0 1.2336697382998756 3.7812472822285157E-4 88-89 0.0 7.562494564457032E-5 0.0 1.4500705202618136 3.7812472822285157E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2105 0.0 49.47823 1 GTATCAA 3390 0.0 43.2089 1 ATCAACG 4410 0.0 32.769962 3 TCAACGC 4450 0.0 32.368572 4 CAACGCA 4540 0.0 31.725702 5 TATCAAC 4635 0.0 31.076626 2 AACGCAG 4730 0.0 30.631397 6 GTGGTAT 1035 0.0 26.650774 1 ACGCAGA 5510 0.0 26.122763 7 CGCAGAG 5505 0.0 25.801323 8 GTACATG 4200 0.0 24.684788 1 TACATGG 4320 0.0 24.099127 2 GCAGAGT 6255 0.0 22.480652 9 ACATGGG 4455 0.0 22.195072 3 TGGTATC 1205 0.0 22.092352 2 ATAAGCG 110 0.0028642656 21.608183 3 GAGTACT 3720 0.0 20.557869 12-13 GTATAGG 305 2.055458E-10 20.270557 1 GTCTAGA 500 0.0 19.974297 1 CAGAGTA 5985 0.0 19.921083 10-11 >>END_MODULE