FastQCFastQC Report
Thu 26 May 2016
SRR937750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322315
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35550.2688466817664475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31420.23761357921523993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.155938637919104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.047.9146351
GTATCAA37300.043.0583041
ATCAACG47400.033.8744623
TCAACGC48400.033.1745764
CAACGCA49150.032.57175
TATCAAC50150.032.206392
AACGCAG51100.031.416966
ACGCAGA58700.026.946817
CGCAGAG60450.025.8523988
GCAGAGT65800.023.17299
GAGTACT41950.022.58978712-13
CCGTCCA1903.2871685E-722.5003939
GTACATG39700.022.1426831
TACATGG39450.021.4341872
ACATGGG39300.021.0324923
GTACTTT44900.020.89243714-15
GTGGTAT11450.020.7497841
CAGAGTA63500.020.4590210-11
GTACACG2108.580173E-720.364431
TGGTATC11900.019.9598432
Produced by FastQC (version 0.11.5)