##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937750_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1322315 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23627804267516 33.0 31.0 34.0 31.0 34.0 2 32.453299705440834 34.0 31.0 34.0 31.0 34.0 3 32.55144802864673 34.0 31.0 34.0 31.0 34.0 4 36.16197350858154 37.0 35.0 37.0 35.0 37.0 5 36.06896616918056 37.0 35.0 37.0 35.0 37.0 6 36.09742837372335 37.0 35.0 37.0 35.0 37.0 7 36.041018214268156 37.0 35.0 37.0 35.0 37.0 8 36.015446395147904 37.0 35.0 37.0 35.0 37.0 9 37.71242328794576 39.0 37.0 39.0 35.0 39.0 10-11 37.63498636860355 39.0 37.0 39.0 35.0 39.0 12-13 37.539678896480794 39.0 37.0 39.0 35.0 39.0 14-15 38.796846439766625 40.0 38.0 41.0 35.0 41.0 16-17 38.74288690667504 40.0 38.0 41.0 35.0 41.0 18-19 38.698138491962965 40.0 38.0 41.0 34.5 41.0 20-21 38.56731262974405 40.0 38.0 41.0 34.0 41.0 22-23 38.4141641741945 40.0 38.0 41.0 34.0 41.0 24-25 38.500401190336646 40.0 38.0 41.0 34.0 41.0 26-27 38.384202326979576 40.0 38.0 41.0 34.0 41.0 28-29 38.24003319935114 40.0 38.0 41.0 34.0 41.0 30-31 38.33175793967398 40.0 38.0 41.0 34.0 41.0 32-33 38.30123268661401 40.0 38.0 41.0 34.0 41.0 34-35 38.193465248446856 40.0 38.0 41.0 34.0 41.0 36-37 38.03976019329736 40.0 38.0 41.0 33.0 41.0 38-39 37.911539988580635 40.0 37.0 41.0 33.0 41.0 40-41 37.75318626802237 40.0 37.0 41.0 33.0 41.0 42-43 37.562659426838536 40.0 37.0 41.0 33.0 41.0 44-45 37.30661453587081 40.0 37.0 41.0 32.0 41.0 46-47 37.02987034103069 40.0 36.5 41.0 31.0 41.0 48-49 36.76907733785066 39.0 36.0 41.0 31.0 41.0 50-51 36.539613480902815 39.0 36.0 41.0 30.5 41.0 52-53 36.29853816980069 39.0 35.5 40.5 30.0 41.0 54-55 36.07356189712739 39.0 35.0 40.0 29.5 41.0 56-57 35.80382586600016 38.5 35.0 40.0 29.0 41.0 58-59 35.52262622748739 38.0 35.0 40.0 28.0 41.0 60-61 35.16594759947516 38.0 34.0 40.0 28.0 41.0 62-63 34.79822583877518 37.5 34.0 40.0 27.5 41.0 64-65 34.402073636009575 37.0 34.0 39.5 26.5 41.0 66-67 33.952281793672455 36.0 33.0 39.0 26.0 40.5 68-69 33.49660973368675 36.0 33.0 39.0 26.0 40.0 70-71 33.076289310792056 35.0 32.0 38.5 25.0 40.0 72-73 32.566296608599316 35.0 32.0 37.5 24.0 39.5 74-75 32.04778475627971 35.0 31.0 37.0 22.5 39.0 76-77 30.81687116912385 33.5 29.5 35.5 21.5 38.0 78-79 31.434089078623476 34.0 31.0 36.0 22.0 38.0 80-81 31.237276291957667 34.0 31.0 36.0 21.5 37.0 82-83 30.87649274189584 34.0 31.0 35.0 20.0 37.0 84-85 30.483984527136123 34.0 30.5 35.0 19.0 36.5 86-87 30.050720138544904 34.0 30.0 35.0 17.5 36.0 88-89 29.671315079992286 34.0 30.0 35.0 12.5 36.0 90-91 29.137283854452228 34.0 29.0 35.0 6.5 35.0 92-93 28.63900961571184 33.5 29.0 35.0 2.0 35.0 94-95 28.136094274057243 33.0 28.5 35.0 2.0 35.0 96-97 27.55992218193093 33.0 27.0 35.0 2.0 35.0 98-99 26.633071544979828 33.0 25.5 34.5 2.0 35.0 100-101 23.938383819286628 30.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3205108423698064 1101 2 0.2999226456047239 1101 3 0.2027657745490572 1101 4 0.06690082897894456 1101 5 0.1399173123374453 1101 6 0.21384589430075351 1101 7 0.20160018644799038 1101 8 0.09253355119145823 1101 9 0.3491356617530528 1101 10-11 0.3279775623018466 1101 12-13 0.31211260246832495 1101 14-15 0.38583266014661177 1101 16-17 0.3260091585495246 1101 18-19 0.3224021782909787 1101 20-21 0.317135083584823 1101 22-23 0.3164534541109987 1101 24-25 0.3940062304110512 1101 26-27 0.33329552291055364 1101 28-29 0.4293305312227389 1101 30-31 0.39490118715156086 1101 32-33 0.4937456580466275 1101 34-35 0.41958247144842886 1101 36-37 0.4751857753457571 1101 38-39 0.44980149047059825 1101 40-41 0.35851139664399057 1101 42-43 0.4791998384600902 1101 44-45 0.23233646362405835 1101 46-47 0.24831769071685272 1101 48-49 0.12937678339600467 1101 50-51 0.12763440943152915 1101 52-53 0.04661551426859489 1101 54-55 0.0882201702247869 1101 56-57 0.108662567300712 1101 58-59 0.027973285054244457 1101 60-61 -0.09612090359917858 1101 62-63 -0.04750092737106115 1101 64-65 -0.11025522534413312 1101 66-67 8.978773294785469E-4 1101 68-69 -0.042151005759116345 1101 70-71 -0.11222123608480672 1101 72-73 0.028065282859664364 1101 74-75 0.11514523420225942 1101 76-77 0.15513598626879244 1101 78-79 0.23167669708717398 1101 80-81 0.13646482088629597 1101 82-83 0.044025201789722246 1101 84-85 0.09036465630310886 1101 86-87 -0.08233833978729521 1101 88-89 0.06609489022025627 1101 90-91 0.05755429196065265 1101 92-93 0.29710316017153815 1101 94-95 0.25275196252832544 1101 96-97 0.3356130231484755 1101 98-99 0.23547936250601254 1101 100-101 0.6011316486319203 1102 1 0.200905943002347 1102 2 0.190703646421035 1102 3 0.1701922609074913 1102 4 0.08607972280371001 1102 5 0.05726934001074824 1102 6 0.14943340767037228 1102 7 0.058028795591525295 1102 8 0.06480697787500134 1102 9 0.08968674480334471 1102 10-11 0.12381053934559105 1102 12-13 0.11410156286434159 1102 14-15 0.15622726080555083 1102 16-17 0.20851816625145148 1102 18-19 0.16301433288281686 1102 20-21 0.2072504653427032 1102 22-23 0.26446647959142666 1102 24-25 0.10644080746376261 1102 26-27 0.18530357108371476 1102 28-29 0.03024015608833963 1102 30-31 0.12376340143650566 1102 32-33 0.07177705171602611 1102 34-35 0.20028447176336783 1102 36-37 0.257267609342378 1102 38-39 0.17440318206351435 1102 40-41 0.2302819526847344 1102 42-43 0.28557338041792946 1102 44-45 0.14834650608442956 1102 46-47 0.3027052394117149 1102 48-49 0.15905208558798734 1102 50-51 0.11080562515705594 1102 52-53 0.030955126242346864 1102 54-55 0.18930922486816115 1102 56-57 -0.038198369481598604 1102 58-59 0.07827203096159252 1102 60-61 -0.04684219728787298 1102 62-63 0.018282047640077792 1102 64-65 0.026794079996292908 1102 66-67 -0.012227597820661629 1102 68-69 -0.04692785632727947 1102 70-71 0.007824761486347143 1102 72-73 0.12416067595339086 1102 74-75 -0.264015819443042 1102 76-77 -0.15938268634335273 1102 78-79 0.2677000285654678 1102 80-81 -0.009994034323689505 1102 82-83 0.047543941220773434 1102 84-85 0.2609958332549809 1102 86-87 8.733878177444865E-4 1102 88-89 0.14316862417729226 1102 90-91 0.1076990740352457 1102 92-93 0.08060127452344545 1102 94-95 0.05473578300883375 1102 96-97 0.15193183435143354 1102 98-99 0.13018468881355005 1102 100-101 0.4266784772339598 1103 1 0.14801893725454818 1103 2 0.1276359276761454 1103 3 0.06771211423257739 1103 4 0.07484853399893154 1103 5 -0.002302430855550597 1103 6 0.048046913139302205 1103 7 0.022192881693150923 1103 8 -0.014620566223840115 1103 9 0.11024090640123774 1103 10-11 0.06132725904792835 1103 12-13 0.11959697571246153 1103 14-15 0.1337297189634583 1103 16-17 0.1423484280558398 1103 18-19 0.039055961992744415 1103 20-21 0.02382136511529609 1103 22-23 0.14035098244707456 1103 24-25 0.09804122961260475 1103 26-27 0.032864174858033834 1103 28-29 0.007541198863627585 1103 30-31 0.08999416515467829 1103 32-33 0.06726045085989085 1103 34-35 0.03387218938656389 1103 36-37 -0.05315667160470383 1103 38-39 -0.07609167661409799 1103 40-41 -0.1254191709237773 1103 42-43 0.03781376489207133 1103 44-45 0.10955991247034547 1103 46-47 0.04315848411337697 1103 48-49 -0.06293593097570493 1103 50-51 -0.03587907764508458 1103 52-53 -0.18305127720890368 1103 54-55 -0.07379490443650383 1103 56-57 -0.1806504559891735 1103 58-59 -0.16219679283044286 1103 60-61 -0.24787388916373487 1103 62-63 -0.32652706621681205 1103 64-65 -0.13111379950701263 1103 66-67 -0.2187718395440541 1103 68-69 -0.3121616472852722 1103 70-71 -0.2639769514123884 1103 72-73 -0.16908320669421784 1103 74-75 -0.07157510567143532 1103 76-77 -0.2053080642809597 1103 78-79 -0.005073374455363933 1103 80-81 -0.026473596115032194 1103 82-83 0.0531328286111048 1103 84-85 0.05140343122186408 1103 86-87 0.15533962370030707 1103 88-89 0.06748206479269214 1103 90-91 0.22196791839167318 1103 92-93 0.2394407836307586 1103 94-95 -0.03835951962834727 1103 96-97 0.23892436217457913 1103 98-99 0.15054233131437655 1103 100-101 0.2996757295275394 1104 1 0.17795944148002718 1104 2 0.13010916469220035 1104 3 0.017586847240401937 1104 4 -0.0335442445359746 1104 5 -0.006872466252218601 1104 6 0.0405137427827853 1104 7 0.062340326022230386 1104 8 0.05666222318644998 1104 9 0.10885828599254666 1104 10-11 0.18161919248689884 1104 12-13 0.048274167708918014 1104 14-15 0.09461858029374781 1104 16-17 0.02189136294910554 1104 18-19 0.04111604001581526 1104 20-21 0.05709484646465057 1104 22-23 0.14126063585527504 1104 24-25 0.008934774437605597 1104 26-27 0.06578122686857313 1104 28-29 0.014214189347555362 1104 30-31 0.12591239177689317 1104 32-33 0.10501865324226856 1104 34-35 0.12102520949304818 1104 36-37 0.07492591445835473 1104 38-39 0.10885491175685047 1104 40-41 0.10906317977718061 1104 42-43 0.07715248182560686 1104 44-45 -0.004033304246171099 1104 46-47 -0.0409909426752435 1104 48-49 -0.08848626359178269 1104 50-51 -0.08369426034755634 1104 52-53 -0.06485922050387671 1104 54-55 -0.07044015895696276 1104 56-57 -0.02365135794669726 1104 58-59 0.10783284291680673 1104 60-61 -0.059210409032523614 1104 62-63 0.06770137310643065 1104 64-65 0.0019858428323047406 1104 66-67 0.06872877062955496 1104 68-69 0.017316928035221224 1104 70-71 0.036355114958666945 1104 72-73 0.042766650580126964 1104 74-75 -0.05665586627097596 1104 76-77 -0.19384647531412824 1104 78-79 0.1442502893304045 1104 80-81 -0.020424631827012263 1104 82-83 0.12771781161199414 1104 84-85 0.2509812130747946 1104 86-87 0.2369357762863622 1104 88-89 0.18684895305682403 1104 90-91 0.21961187868443588 1104 92-93 0.25302929486433 1104 94-95 0.02518648834106685 1104 96-97 0.28843066026560393 1104 98-99 0.8131442522948049 1104 100-101 0.8546425866563219 1105 1 0.10042196725180474 1105 2 0.14938697306609328 1105 3 0.11996716493182902 1105 4 0.029730628414966986 1105 5 -0.019182027982026284 1105 6 -0.024402044226746966 1105 7 0.007946595955750979 1105 8 0.03298218248384188 1105 9 0.13385358381303547 1105 10-11 0.06912073168954436 1105 12-13 0.037610932618434845 1105 14-15 0.04558668801493582 1105 16-17 0.029669015936832466 1105 18-19 0.023132740533654328 1105 20-21 0.1066215388320515 1105 22-23 -0.03791473040729443 1105 24-25 -0.07462802976634464 1105 26-27 -0.12300349991111403 1105 28-29 -0.013310813392564569 1105 30-31 0.04451642405932432 1105 32-33 0.0750228073979855 1105 34-35 0.07959641447335031 1105 36-37 0.127590550505154 1105 38-39 0.11692506962829441 1105 40-41 0.06793185117354028 1105 42-43 0.0018682198512891546 1105 44-45 0.07924060346120854 1105 46-47 0.0993881725211665 1105 48-49 0.08897444219973494 1105 50-51 0.10659178101267486 1105 52-53 0.2813914622706335 1105 54-55 0.14576921229511441 1105 56-57 0.18835852722991575 1105 58-59 0.2760177384788065 1105 60-61 0.37370583985056527 1105 62-63 0.20422853501521132 1105 64-65 0.23186288252952636 1105 66-67 0.2863463149687391 1105 68-69 0.2782784188764609 1105 70-71 0.2494565372495714 1105 72-73 0.2829237597568408 1105 74-75 0.19985377691816808 1105 76-77 0.09869426675971127 1105 78-79 0.2242380839608522 1105 80-81 0.28311763163306125 1105 82-83 0.3802410890920065 1105 84-85 0.3768340892074349 1105 86-87 0.4879981080171234 1105 88-89 0.46955390831958965 1105 90-91 0.4814355485314472 1105 92-93 0.6494830170735462 1105 94-95 0.7117441636737283 1105 96-97 0.797091917057827 1105 98-99 0.8538991810133716 1105 100-101 0.9480350885180258 1106 1 0.006120800828760764 1106 2 0.0043608732374949 1106 3 0.05611858103766565 1106 4 -0.041523573156581506 1106 5 -0.0810675597358852 1106 6 0.022495563567211718 1106 7 0.016116009256833763 1106 8 -0.029613349937825717 1106 9 -0.05847775972525682 1106 10-11 -0.1689782936942592 1106 12-13 -0.24080903532719589 1106 14-15 0.007102889571648063 1106 16-17 0.004238841789572234 1106 18-19 -0.01099436210711957 1106 20-21 -0.0962443410679299 1106 22-23 -0.06125084314668783 1106 24-25 0.13176628911531196 1106 26-27 0.2142746422708015 1106 28-29 0.16639285987542962 1106 30-31 0.22640412616141248 1106 32-33 0.20293770563276325 1106 34-35 0.08180826828462529 1106 36-37 -0.055682371711050394 1106 38-39 -0.011422043252068192 1106 40-41 0.0340702451900583 1106 42-43 -0.11197164555002104 1106 44-45 0.09184044875433273 1106 46-47 0.09222764651779158 1106 48-49 0.2792632005379474 1106 50-51 0.4307012889928856 1106 52-53 0.41003722990609504 1106 54-55 0.342160683754102 1106 56-57 0.2182550739647482 1106 58-59 0.05738877712956025 1106 60-61 0.28347106196807914 1106 62-63 0.23397737749616 1106 64-65 0.22570938882539338 1106 66-67 -0.014055616826674111 1106 68-69 -0.014304694330384393 1106 70-71 0.18993218668454404 1106 72-73 0.18375994669616347 1106 74-75 -0.11061268099933486 1106 76-77 0.06164229526732612 1106 78-79 0.24072345544285056 1106 80-81 0.20883746379280055 1106 82-83 0.01726820360477177 1106 84-85 -0.010226183403144518 1106 86-87 0.15148144271078934 1106 88-89 0.04129296011856809 1106 90-91 0.25378920770144475 1106 92-93 0.19055361089565892 1106 94-95 0.38674935766946916 1106 96-97 0.40876978173198 1106 98-99 0.4957798990461839 1106 100-101 0.5170046275880047 1107 1 0.16930252651049216 1107 2 0.2125161438927634 1107 3 0.17580213822122204 1107 4 0.11954683791382337 1107 5 0.09958974592142056 1107 6 0.158611762183412 1107 7 0.17586366900449235 1107 8 0.096124338299866 1107 9 0.22186920812170996 1107 10-11 0.18476865380268492 1107 12-13 0.15441379739564098 1107 14-15 0.18735319482195223 1107 16-17 0.2247821873329201 1107 18-19 0.3588291014663483 1107 20-21 0.2926699853464072 1107 22-23 0.2304539798081393 1107 24-25 0.19163350523253087 1107 26-27 0.20514257063873487 1107 28-29 0.12690110163367052 1107 30-31 0.2469322091894952 1107 32-33 0.08316511711017682 1107 34-35 -0.016444173717815147 1107 36-37 0.04906935804068269 1107 38-39 0.054168616088588806 1107 40-41 -0.056207532587720266 1107 42-43 0.034879188800815086 1107 44-45 0.12143700960089632 1107 46-47 -0.06875373321359035 1107 48-49 0.11662838165312905 1107 50-51 -0.01809297230138185 1107 52-53 -0.10142237530350684 1107 54-55 -0.1446626780692597 1107 56-57 -0.18129213808246192 1107 58-59 -0.14594608245530338 1107 60-61 -0.050536365789341176 1107 62-63 -0.12595088868211235 1107 64-65 -0.0848573592413544 1107 66-67 -0.09773235675341851 1107 68-69 -0.11380357507926675 1107 70-71 -0.2123495593472029 1107 72-73 -0.030080610894387405 1107 74-75 -0.08161390200055507 1107 76-77 -0.09827070839567753 1107 78-79 -0.049416959697570206 1107 80-81 0.06768463513997247 1107 82-83 0.014126669213240461 1107 84-85 0.035663112486151505 1107 86-87 0.14728425101359832 1107 88-89 0.02306697314257633 1107 90-91 0.37772083163306647 1107 92-93 0.3795544248964724 1107 94-95 0.57976930068941 1107 96-97 0.600950286412484 1107 98-99 0.9068630288793145 1107 100-101 0.9032861701195465 1108 1 0.08856555593722959 1108 2 0.09638915532375592 1108 3 -0.008530872510149834 1108 4 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>>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 11.0 5 21.0 6 48.0 7 95.0 8 265.0 9 521.0 10 870.0 11 1438.0 12 2140.0 13 3169.0 14 4169.0 15 5535.0 16 7123.0 17 9936.0 18 9141.0 19 6327.0 20 5895.0 21 6010.0 22 6370.0 23 7155.0 24 8549.0 25 10254.0 26 12657.0 27 15821.0 28 19696.0 29 24993.0 30 31127.0 31 39998.0 32 50770.0 33 67120.0 34 92496.0 35 132786.0 36 203148.0 37 298611.0 38 219497.0 39 18544.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32757727338397 19.41390001868343 15.060282429935242 23.198240277997346 2 15.793816148194646 19.92785380185508 39.961204402884334 24.31712564706594 3 19.60901903101757 23.190313956961845 29.846519172814347 27.354147839206238 4 13.423448154861973 16.572173154162442 36.2852063013825 33.71917238959308 5 14.168422247016773 36.94729476729893 34.29489967662021 14.589383309064088 6 33.35665102490707 35.67984935510828 16.772100445052804 14.191399174931844 7 29.46816756975456 32.13666940176887 19.901385070879478 18.493777957597093 8 26.452471612286026 35.97720664138272 18.976340735755095 18.59398101057615 9 26.12123435036281 16.80991291787509 19.959540654080154 37.10931207768194 10-11 25.282440265746057 26.538494987956728 26.950121567100123 21.228943179197092 12-13 25.776074805087944 23.56025238549366 27.89123646354772 22.772436345870677 14-15 22.616169369628267 25.41334704665681 25.617534399897153 26.352949183817774 16-17 22.435614811901853 28.70715374173325 26.43556943693447 22.42166200943043 18-19 22.365812987071916 27.302042251657134 28.698986247603635 21.63315851366732 20-21 22.803303297625753 26.444909117721572 29.020089766810482 21.731697817842193 22-23 22.761654837575296 26.168735654784637 28.741515762248497 22.32809374539157 24-25 22.56308065778578 26.381497600798596 28.849706764273265 22.20571497714236 26-27 22.501296026916524 26.69078102306531 28.61767338306699 22.19024956695118 28-29 22.40421930047126 26.687801485966357 28.82558442476752 22.082394788794865 30-31 22.491690709097302 26.552977165047665 28.680798448176116 22.27453367767892 32-33 22.348154562263908 26.5949490098804 28.6388644158162 22.41803201203949 34-35 22.79381236694736 26.644596786696063 28.413199578012804 22.148391268343776 36-37 22.770368633797545 26.476482532528177 28.48492983895668 22.268218994717596 38-39 22.599842926928503 26.646799985933754 28.559469021930866 22.193888065206878 40-41 22.708821826726123 26.602021998698483 28.382019132492452 22.30713704208294 42-43 22.55960329643358 26.604621224412305 28.386489478256298 22.449286000897818 44-45 22.712439925433802 26.84961601433849 28.19502160982822 22.242922450399487 46-47 22.761104577956086 27.121374256512254 27.828732185598742 22.28878897993292 48-49 22.910804157859513 26.955528750713707 27.73488162805383 22.398785463372946 50-51 22.90014104052363 27.160358915992028 27.618041087032967 22.321458956451377 52-53 23.136137431753188 27.20211417177986 27.341264124381908 22.320484272085046 54-55 22.90732541035986 27.19340701723871 27.52006896995043 22.379198602451 56-57 22.840397333464416 27.45529620400585 27.516363347613844 22.187943114915885 58-59 22.89420448229053 27.119937382545007 27.54109270483962 22.444765430324846 60-61 22.91776165285881 27.029225260244345 27.826009687555537 22.227003399341307 62-63 22.759667703988836 27.226076993757157 27.747284119139536 22.26697118311446 64-65 22.73036303755157 27.142057679146042 27.545441139214184 22.58213814408821 66-67 22.678181825056814 27.32155348763343 27.494658988213853 22.505605699095906 68-69 22.696256187065867 27.215489501366918 27.57353580652114 22.514718505046076 70-71 22.71020898953729 27.235416674544265 27.443498712485297 22.610875623433145 72-73 22.89870416655638 27.13022237515267 27.332783792061647 22.6382896662293 74-75 22.825272344335502 27.466110571233028 27.140280492923395 22.568336591508075 76-77 22.768402385210788 27.304689124754695 27.312894431357126 22.614014058677395 78-79 22.89968729084976 27.323368486328903 27.30378162540696 22.473162597414383 80-81 22.64620959923286 27.509275406597826 27.304218211408184 22.54029678276113 82-83 22.812016223505026 27.50852883544244 27.169341719863958 22.51011322118857 84-85 22.893822271916534 27.400379564042073 27.099995349035943 22.605802815005447 86-87 22.78371643670381 27.386969065615986 27.236097299055068 22.593217198625137 88-89 22.78734643409475 27.336980976544922 27.15733391816625 22.71833867119408 90-91 22.749244415938815 27.446420845951614 27.16831448028826 22.636020257821315 92-93 22.89216034752147 27.096457779680215 27.338691258570435 22.672690614227868 94-95 22.677803700328592 27.306012561303472 27.283060390300346 22.733123348067593 96-97 22.732329286138324 27.18210108786484 27.3296453568174 22.75592426917943 98-99 22.71100624293369 27.1890916928522 27.07262751504014 23.027274549173974 100-101 22.737398179041506 27.16442103973296 27.28688227609979 22.811298505125748 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 108.0 1 83.5 2 75.5 3 103.0 4 98.0 5 92.0 6 181.5 7 406.0 8 668.0 9 817.5 10 800.0 11 794.0 12 926.5 13 1050.0 14 1164.5 15 1256.5 16 1299.5 17 1430.5 18 1561.5 19 1678.5 20 1832.0 21 2115.5 22 2568.0 23 3246.0 24 4130.0 25 5194.0 26 6756.5 27 8538.0 28 10194.5 29 12098.5 30 14254.5 31 16497.0 32 18806.5 33 20796.0 34 23192.0 35 25528.0 36 28658.5 37 31786.0 38 33797.5 39 35631.0 40 37399.0 41 40840.5 42 44050.0 43 46770.0 44 49827.5 45 52253.0 46 53427.0 47 53790.0 48 54057.5 49 53575.0 50 53554.0 51 52418.0 52 49608.0 53 47864.0 54 46733.5 55 43425.5 56 38344.5 57 34058.5 58 30605.0 59 26394.5 60 21452.0 61 17090.0 62 13465.5 63 10324.0 64 7977.5 65 6071.0 66 4351.0 67 3490.5 68 2804.5 69 1971.5 70 1281.5 71 800.0 72 575.5 73 445.0 74 354.5 75 278.5 76 165.5 77 94.0 78 67.0 79 36.0 80 19.5 81 13.0 82 11.0 83 9.0 84 6.5 85 3.5 86 0.0 87 0.0 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02177998434563625 2 0.0 3 0.0 4 1.5124989128914064E-4 5 0.025712481519153906 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003932497173517656 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.562494564457032E-4 24-25 0.0 26-27 3.403122554005664E-4 28-29 7.940619292679884E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.781247282228516E-5 40-41 7.184369836234181E-4 42-43 0.016599675568983185 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.781247282228516E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.562494564457032E-5 82-83 0.0027224980432045314 84-85 6.428120379788477E-4 86-87 0.0 88-89 0.0 90-91 0.01202436635748668 92-93 0.0027981229888491017 94-95 0.0 96-97 0.0 98-99 0.0015124989128914065 100-101 0.019964985650166564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1322315.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.86061280101993 #Duplication Level Percentage of deduplicated Percentage of total 1 78.91219023000744 48.026442548689104 2 12.177445947623648 14.822536454473441 3 3.7920321412721303 6.923561996369568 4 1.6696098129146735 4.064539054103331 5 0.914910353067928 2.78410023728558 6 0.5481843937907965 2.0017702880438097 7 0.3674607923259478 1.5654722300913855 8 0.2751686898532297 1.3397548070497085 9 0.19555877680245554 1.071164429533379 >10 1.0637082900634456 11.594075568601157 >50 0.05753364677230642 2.3900654986938235 >100 0.025079537649731688 2.4847740635433797 >500 7.449252375501218E-4 0.2699039303885564 >1k 3.724626187750609E-4 0.661838893133641 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3555 0.2688466817664475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3142 0.23761357921523993 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.155938637919104 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.1343741846685548E-4 0.0 24-25 0.0 0.0 0.0 1.5124989128914064E-4 0.0 26-27 0.0 0.0 0.0 1.5124989128914064E-4 0.0 28-29 0.0 0.0 0.0 3.403122554005664E-4 0.0 30-31 0.0 0.0 0.0 0.00117218665749084 0.0 32-33 0.0 0.0 0.0 0.0019284361139365433 0.0 34-35 0.0 0.0 0.0 0.002646873097559961 0.0 36-37 0.0 0.0 0.0 0.0037812472822285163 0.0 38-39 0.0 0.0 0.0 0.0052559337222976375 0.0 40-41 0.0 0.0 0.0 0.007411244673167891 0.0 42-43 0.0 0.0 0.0 0.010360617553306133 0.0 44-45 0.0 0.0 0.0 0.01678873793309461 0.0 46-47 0.0 0.0 0.0 0.02938029138291557 0.0 48-49 0.0 0.0 0.0 0.038984659479776 0.0 50-51 0.0 0.0 0.0 0.05153840045677467 0.0 52-53 0.0 0.0 0.0 0.06787338871600186 0.0 54-55 0.0 0.0 0.0 0.08848118640414727 0.0 56-57 0.0 0.0 0.0 0.10840835958149156 0.0 58-59 0.0 0.0 0.0 0.13544427764942546 0.0 60-61 0.0 0.0 0.0 0.16671519267345525 0.0 62-63 0.0 0.0 0.0 0.20263704185462617 0.0 64-65 0.0 0.0 0.0 0.23882357834555307 0.0 66-67 0.0 0.0 0.0 0.2938029138291557 0.0 68-69 0.0 0.0 0.0 0.35755474300752843 0.0 70-71 0.0 0.0 0.0 0.4053875211277192 0.0 72-73 0.0 0.0 0.0 0.484264339435006 0.0 74-75 0.0 0.0 0.0 0.556523974998393 0.0 76-77 0.0 0.0 0.0 0.6257208002631749 0.0 78-79 0.0 0.0 0.0 0.7129541750641867 0.0 80-81 0.0 0.0 0.0 0.8056703584244298 0.0 82-83 0.0 0.0 0.0 0.9153643420818791 0.0 84-85 0.0 0.0 0.0 1.054173929812488 0.0 86-87 0.0 0.0 0.0 1.2416481700653779 0.0 88-89 0.0 0.0 0.0 1.4624351988747009 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2390 0.0 47.914635 1 GTATCAA 3730 0.0 43.058304 1 ATCAACG 4740 0.0 33.874462 3 TCAACGC 4840 0.0 33.174576 4 CAACGCA 4915 0.0 32.5717 5 TATCAAC 5015 0.0 32.20639 2 AACGCAG 5110 0.0 31.41696 6 ACGCAGA 5870 0.0 26.94681 7 CGCAGAG 6045 0.0 25.852398 8 GCAGAGT 6580 0.0 23.1729 9 GAGTACT 4195 0.0 22.589787 12-13 CCGTCCA 190 3.2871685E-7 22.500393 9 GTACATG 3970 0.0 22.142683 1 TACATGG 3945 0.0 21.434187 2 ACATGGG 3930 0.0 21.032492 3 GTACTTT 4490 0.0 20.892437 14-15 GTGGTAT 1145 0.0 20.749784 1 CAGAGTA 6350 0.0 20.45902 10-11 GTACACG 210 8.580173E-7 20.36443 1 TGGTATC 1190 0.0 19.959843 2 >>END_MODULE