FastQCFastQC Report
Thu 26 May 2016
SRR937749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254595
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.2502002638301603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.20460786150112187No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18810.14992886150510723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16220.12928474926171393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.048.5506441
TAGTCCG405.378161E-447.5182655
GTATCAA34550.040.72821
ATCAACG43000.032.598443
TCAACGC44350.031.4990144
CAACGCA45050.031.0108095
AACGCAG46200.030.5389146
TATCAAC45600.030.535012
ACGCAGA53350.026.3570237
CGCAGAG54200.025.7683818
TACATGG39150.024.3983022
GTACATG40800.024.1191371
GCAGAGT58700.023.2245989
ACATGGG39750.023.1903783
GTGGTAT10300.023.0772651
TGGTATC11000.021.6009392
CCGTGCG1553.906031E-521.4521479
GAGTACT34350.021.43387412-13
CAGAGTA58100.020.27548610-11
GTACTTT36500.020.23599414-15
Produced by FastQC (version 0.11.5)