FastQCFastQC Report
Thu 26 May 2016
SRR937749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254595
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32650.2602433454620814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.23760655829171964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19660.1567039562568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.053.5692061
GTATCAA32700.040.684391
ATCAACG42600.030.2173253
TCAACGC43700.029.4567094
CAACGCA44750.028.8716895
TATCAAC45350.028.5944482
AACGCAG46200.028.0661226
GTACATG38550.025.020111
TACATGG38100.024.1865042
ACGCAGA55450.023.4717457
CGCAGAG56200.022.9894688
ACATGGG37750.022.9008063
TGTACCG1302.9415917E-421.9232145
GAGTACT38750.020.7775512-13
GGACCGA3001.6734703E-1020.5818236
GCAGAGT62200.020.399
CAGAGTA59800.019.46082710-11
GTACTTT42100.019.17911514-15
AGAGTAC59800.019.06366710-11
GATCGTG1250.006031223619.000127
Produced by FastQC (version 0.11.5)