FastQCFastQC Report
Thu 26 May 2016
SRR937748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016988
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25560.2513303991787514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21180.20826204438990434No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14910.1466093995209383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13780.13549815730372433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.049.6533621
GTATCAA26750.046.0483131
ATCAACG34450.035.3226173
TCAACGC35450.034.1938064
CAACGCA35850.033.678035
TATCAAC36300.033.5224272
AACGCAG37400.032.263196
GTGGTAT6050.028.2998981
ACGCAGA43400.027.3657
CGCAGAG43800.026.8981728
GCAGAGT47450.024.225999
TACATGG30550.024.116962
GTACATG31450.024.0444151
CGCGTTA500.00165364923.75106670-71
TGGTATC7100.022.7626422
ACCGTCC1302.9388178E-421.9256828
ACATGGG31250.021.9035533
CCTATAC2005.348138E-721.3901883
CGTAAGA1353.7773588E-421.1261122
GAGTACT29550.021.05536312-13
Produced by FastQC (version 0.11.5)