##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937748_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1016988 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68482223979044 33.0 31.0 34.0 30.0 34.0 2 31.800165783667065 33.0 31.0 34.0 30.0 34.0 3 31.84986548513847 33.0 31.0 34.0 30.0 34.0 4 35.45146747060929 37.0 35.0 37.0 33.0 37.0 5 35.441629596416085 37.0 35.0 37.0 33.0 37.0 6 35.49612089818169 37.0 35.0 37.0 33.0 37.0 7 35.40260652043092 37.0 35.0 37.0 33.0 37.0 8 35.39714922889946 37.0 35.0 37.0 33.0 37.0 9 36.96811073483659 39.0 37.0 39.0 33.0 39.0 10-11 36.872544218810845 39.0 37.0 39.0 33.0 39.0 12-13 36.76410832772855 39.0 37.0 39.0 33.0 39.0 14-15 37.91298717388996 40.0 38.0 41.0 33.0 41.0 16-17 37.810676232167935 40.0 38.0 41.0 33.0 41.0 18-19 37.697890732240694 40.0 38.0 41.0 32.0 41.0 20-21 37.68416982304609 40.0 37.5 41.0 32.0 41.0 22-23 37.69051158912396 40.0 38.0 41.0 32.0 41.0 24-25 37.62252701113484 40.0 37.0 41.0 32.0 41.0 26-27 37.50513870370152 40.0 37.0 41.0 32.0 41.0 28-29 37.33871097790731 40.0 37.0 41.0 31.5 41.0 30-31 37.102431887101915 39.5 37.0 41.0 31.0 41.0 32-33 36.992741802263154 39.0 36.5 41.0 31.0 41.0 34-35 36.98933271582359 40.0 36.5 41.0 31.0 41.0 36-37 36.82308542480344 39.5 36.0 41.0 30.0 41.0 38-39 36.65101210633754 39.0 36.0 41.0 30.0 41.0 40-41 36.412598280412354 39.0 35.5 41.0 30.0 41.0 42-43 36.15749202547129 39.0 35.0 41.0 29.5 41.0 44-45 35.915871672035465 39.0 35.0 40.5 28.5 41.0 46-47 35.593200214751796 39.0 35.0 40.0 27.5 41.0 48-49 35.26104880293573 39.0 35.0 40.0 26.0 41.0 50-51 34.3955675976511 37.5 33.5 39.5 25.0 40.5 52-53 34.51114319932978 38.0 34.0 39.5 25.5 40.5 54-55 34.74413021589242 38.0 34.0 40.0 25.0 41.0 56-57 34.477490884848194 38.0 34.0 40.0 23.5 41.0 58-59 34.17140762722864 38.0 34.0 40.0 23.0 41.0 60-61 33.78862238295831 37.5 33.0 40.0 22.0 41.0 62-63 33.379238988070654 37.0 33.0 40.0 21.0 41.0 64-65 32.91751426762164 36.5 32.0 39.0 19.5 41.0 66-67 32.38572824851424 36.0 31.5 39.0 16.5 40.0 68-69 31.893849779938407 35.0 31.0 38.5 13.0 40.0 70-71 31.401245639083253 35.0 31.0 38.0 8.0 40.0 72-73 30.883610721070454 35.0 30.0 37.0 7.0 39.5 74-75 30.30295391882697 34.5 29.5 37.0 3.5 39.0 76-77 29.73708244345066 34.0 29.0 36.0 2.0 39.0 78-79 29.17306202236408 34.0 29.0 36.0 2.0 38.0 80-81 28.596841850641304 34.0 27.5 35.0 2.0 37.0 82-83 28.012173693298248 33.5 26.5 35.0 2.0 36.5 84-85 27.42982070584904 33.0 26.0 35.0 2.0 36.0 86-87 26.801120563861126 32.5 25.0 35.0 2.0 36.0 88-89 26.214876675044348 32.0 24.0 35.0 2.0 35.0 90-91 25.61480666438542 32.0 21.5 34.0 2.0 35.0 92-93 24.898760850668836 31.0 19.0 34.0 2.0 35.0 94-95 24.067805126510834 31.0 11.0 34.0 2.0 35.0 96-97 23.118878492174932 31.0 2.0 34.0 2.0 35.0 98-99 21.849731756913553 30.0 2.0 34.0 2.0 35.0 100-101 18.708731568120765 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.011067606316402134 1101 2 -0.1312936489211758 1101 3 -0.12158688297370546 1101 4 0.08867484126367486 1101 5 -0.07518953869875844 1101 6 0.032025356002179706 1101 7 -0.10645559772377311 1101 8 0.006906028452654311 1101 9 0.09666145979633001 1101 10-11 0.0843985904133433 1101 12-13 0.13266314427622916 1101 14-15 0.1742769140243965 1101 16-17 0.13730149276037196 1101 18-19 0.18946980497901222 1101 20-21 0.25675108179166983 1101 22-23 0.157651702005829 1101 24-25 0.20751058750133922 1101 26-27 0.14399157662330708 1101 28-29 0.2136233354160808 1101 30-31 0.11729538669609951 1101 32-33 0.13995594294966196 1101 34-35 0.15004371475369993 1101 36-37 0.2417765791128943 1101 38-39 0.1277329919103849 1101 40-41 0.3263931598567211 1101 42-43 0.14998840809802516 1101 44-45 0.15741253111894338 1101 46-47 0.24513323282186406 1101 48-49 0.3805051181937458 1101 50-51 0.21634669485196412 1101 52-53 0.3867145757530537 1101 54-55 0.305041689393974 1101 56-57 0.5562416432899298 1101 58-59 0.5594952293268776 1101 60-61 0.5219486371114215 1101 62-63 0.6443022771225273 1101 64-65 0.6869604263676408 1101 66-67 0.5569671286550104 1101 68-69 0.7064601581602652 1101 70-71 0.7255864194662678 1101 72-73 0.6431666569988899 1101 74-75 0.884914686669628 1101 76-77 0.4694482912680513 1101 78-79 1.0126178919261406 1101 80-81 1.0457066666730626 1101 82-83 1.113929913938776 1101 84-85 1.3512864402679874 1101 86-87 1.5269125100498933 1101 88-89 1.7955579707971019 1101 90-91 1.6987287793842611 1101 92-93 1.6882520000234926 1101 94-95 1.9094921940345522 1101 96-97 1.9028939266410276 1101 98-99 2.4013630046712535 1101 100-101 2.7807286288408513 1102 1 0.015443378971831123 1102 2 -0.0011432647451563582 1102 3 -0.006997192348851655 1102 4 0.04172868850089628 1102 5 -0.1544385567417308 1102 6 -0.048395359770161406 1102 7 -0.09617439026872177 1102 8 0.03645471943742962 1102 9 0.23815912031304975 1102 10-11 0.13470823941261045 1102 12-13 -0.0444617559631979 1102 14-15 0.13384943018169793 1102 16-17 0.17095503797734324 1102 18-19 0.05215622032726941 1102 20-21 0.08124582382256307 1102 22-23 0.11259513189174442 1102 24-25 0.025633367339530366 1102 26-27 0.08936483765956638 1102 28-29 0.03392226504379181 1102 30-31 0.3487721952966396 1102 32-33 0.31868972696606335 1102 34-35 0.348736460008233 1102 36-37 0.37921730959502753 1102 38-39 0.3187001609008817 1102 40-41 0.3589855264122832 1102 42-43 0.5392492800445723 1102 44-45 0.5100749648074938 1102 46-47 0.42462108941700905 1102 48-49 0.4718505949319294 1102 50-51 0.24318620160438797 1102 52-53 0.35614187181113977 1102 54-55 0.5603573631495067 1102 56-57 0.5594731034717668 1102 58-59 0.7661443694298455 1102 60-61 0.7587166683462669 1102 62-63 0.5603371268165986 1102 64-65 0.8426475615569089 1102 66-67 1.0830652774117198 1102 68-69 0.9543749665417174 1102 70-71 1.176654835659555 1102 72-73 1.1179409242476872 1102 74-75 1.094287681677688 1102 76-77 1.2495769628484155 1102 78-79 1.4423395012402551 1102 80-81 1.5439784614244836 1102 82-83 1.6203106785351125 1102 84-85 1.7186117223361173 1102 86-87 1.6269938833813455 1102 88-89 1.5855590270855409 1102 90-91 1.9310075847611579 1102 92-93 1.8230559215921183 1102 94-95 1.7865480443076223 1102 96-97 1.7561236263421343 1102 98-99 1.7805578390295764 1102 100-101 2.399177684440744 1103 1 -0.01971535676401004 1103 2 -0.06488448567516159 1103 3 0.05997798616053274 1103 4 -0.08719529714807805 1103 5 -0.08085931184911033 1103 6 -0.13961814416518337 1103 7 -0.12249891232424659 1103 8 -0.061063717097560755 1103 9 -0.12016699066141712 1103 10-11 0.020829452066188026 1103 12-13 0.05807743997354464 1103 14-15 0.15310702049045943 1103 16-17 0.13775146310490527 1103 18-19 0.03242942069112331 1103 20-21 0.07165838643260969 1103 22-23 0.03526123638561529 1103 24-25 -0.00953073636573265 1103 26-27 0.04507884591257749 1103 28-29 -0.09682317023020204 1103 30-31 -0.2225151706505315 1103 32-33 0.08655628119817038 1103 34-35 -0.08220924932501106 1103 36-37 0.04321707145238207 1103 38-39 -0.05170534526935455 1103 40-41 -0.02100082918543933 1103 42-43 -0.0018696097422292723 1103 44-45 0.17488943802425894 1103 46-47 -0.08032451841280164 1103 48-49 0.28361835510276734 1103 50-51 0.21736041270807505 1103 52-53 0.2672456141642172 1103 54-55 0.24640020344948965 1103 56-57 0.2638556113090047 1103 58-59 0.25870289981646266 1103 60-61 0.2887472852918691 1103 62-63 0.41767504341981976 1103 64-65 0.39133358024134424 1103 66-67 0.7416487581202134 1103 68-69 0.6844549200063064 1103 70-71 0.7629361602011357 1103 72-73 0.5081439896510318 1103 74-75 0.6632980525946159 1103 76-77 0.6893876296868555 1103 78-79 1.0017136350451885 1103 80-81 1.1970126817415654 1103 82-83 1.2967411935869038 1103 84-85 1.4373328085008126 1103 86-87 1.2396919614530937 1103 88-89 1.5167921667815776 1103 90-91 1.6223608108131842 1103 92-93 1.5756764666654952 1103 94-95 1.6559687720871565 1103 96-97 1.724080996856955 1103 98-99 2.13197464135947 1103 100-101 2.746374530807511 1104 1 0.1268721953000096 1104 2 0.03203847093037737 1104 3 0.048273263250848686 1104 4 -0.07165795611520309 1104 5 -0.12792644573594458 1104 6 -0.06671363417873266 1104 7 -0.014230982245813095 1104 8 -0.021160793944176248 1104 9 -0.057825894712259185 1104 10-11 -0.048790389149601765 1104 12-13 0.052634779139559384 1104 14-15 0.08742903407819114 1104 16-17 0.20259920029103995 1104 18-19 0.0010620953247553189 1104 20-21 -2.1430364048313777E-4 1104 22-23 0.040215485642022486 1104 24-25 0.04799284538644599 1104 26-27 0.05503780016687898 1104 28-29 0.07251739845645488 1104 30-31 0.03680514005592528 1104 32-33 -0.047085841273052154 1104 34-35 0.17428478302216632 1104 36-37 -0.018160319834414906 1104 38-39 0.1585101764303971 1104 40-41 0.31368421346228104 1104 42-43 0.13381016784886413 1104 44-45 0.3533435750166447 1104 46-47 0.382291309048739 1104 48-49 0.5069150323399114 1104 50-51 0.39483596059436366 1104 52-53 0.3677695970395476 1104 54-55 0.4489373704100643 1104 56-57 0.5227614280991588 1104 58-59 0.7069451479224753 1104 60-61 0.6809327000506755 1104 62-63 0.7718723304146025 1104 64-65 0.8036407447043601 1104 66-67 0.7490213510659984 1104 68-69 0.7854128222583903 1104 70-71 0.8820866368817519 1104 72-73 0.8359102887476197 1104 74-75 0.9101219898074895 1104 76-77 0.9920650636087558 1104 78-79 1.0404089000261294 1104 80-81 1.1169911371399515 1104 82-83 1.2377743253495481 1104 84-85 1.4670045686745858 1104 86-87 1.5502679894257554 1104 88-89 1.6476148224186211 1104 90-91 1.630533884097627 1104 92-93 1.6580739721336322 1104 94-95 1.7436815376420327 1104 96-97 2.1849386940064583 1104 98-99 2.6327165930380083 1104 100-101 2.9420789812561843 1105 1 0.08759421696066028 1105 2 0.12313212138694851 1105 3 0.02760079209632238 1105 4 9.717600311844876E-4 1105 5 -0.1828085863178046 1105 6 -0.012904615986620627 1105 7 -0.15205783862013078 1105 8 2.3936178598660263E-4 1105 9 0.13615725811565937 1105 10-11 0.17054704979710067 1105 12-13 0.09018415267959057 1105 14-15 0.07310044343616084 1105 16-17 0.17036871965112965 1105 18-19 0.050226107500016326 1105 20-21 0.2428295131642173 1105 22-23 0.16367411096941709 1105 24-25 0.3442892989859345 1105 26-27 0.1532072628978156 1105 28-29 0.11220876958974912 1105 30-31 0.18844384607985631 1105 32-33 0.26792513062473233 1105 34-35 0.2904778884231689 1105 36-37 0.35438162112969707 1105 38-39 0.23290105913726933 1105 40-41 0.26102901419845637 1105 42-43 0.2270472316274379 1105 44-45 0.4393873210349071 1105 46-47 0.4649231487882446 1105 48-49 0.3745387316391202 1105 50-51 0.31400775927773594 1105 52-53 0.26786303513680565 1105 54-55 0.3240332860326234 1105 56-57 0.46026125113306904 1105 58-59 0.7023383665817775 1105 60-61 0.7213744074195461 1105 62-63 0.7693863107359746 1105 64-65 0.854131294714982 1105 66-67 1.1944181090471702 1105 68-69 0.8883929312695109 1105 70-71 0.8567068676455349 1105 72-73 0.8000994301081334 1105 74-75 0.913780232888115 1105 76-77 0.9816891876265927 1105 78-79 1.0924638303014866 1105 80-81 1.1829895798383312 1105 82-83 1.2968710904093612 1105 84-85 1.5612864402679847 1105 86-87 1.6321225940835014 1105 88-89 1.6454459259791783 1105 90-91 1.8096391435299175 1105 92-93 1.595997098062707 1105 94-95 1.6799963957152215 1105 96-97 1.9518715176774428 1105 98-99 2.29723135201019 1105 100-101 2.675392494387072 1106 1 -0.21670958050769684 1106 2 -0.3089751012489401 1106 3 -0.24510211185006625 1106 4 -0.15074826975288147 1106 5 -0.2007683778293483 1106 6 -0.18285249387194824 1106 7 -0.06291413050396955 1106 8 -0.11339677621351285 1106 9 -0.15245033682104747 1106 10-11 -0.4671943252339261 1106 12-13 -0.721089116121604 1106 14-15 -0.7959142003960551 1106 16-17 -0.6438432436151871 1106 18-19 -0.12090320498446516 1106 20-21 -0.11474308549549761 1106 22-23 -0.21373963934083662 1106 24-25 -0.5705679857000874 1106 26-27 -0.274933715012331 1106 28-29 -0.062186862080643834 1106 30-31 0.08504349859908444 1106 32-33 0.19532150689676087 1106 34-35 0.11559081967161688 1106 36-37 -0.2133026677757357 1106 38-39 -0.24114709176866 1106 40-41 -0.10416830523564613 1106 42-43 0.17151980546709922 1106 44-45 0.0913654793868588 1106 46-47 0.05663821456138862 1106 48-49 0.16172165544156059 1106 50-51 0.14081838226110222 1106 52-53 0.1772350877553137 1106 54-55 0.16638622720910234 1106 56-57 -0.06674793228713582 1106 58-59 -0.12604088385256773 1106 60-61 0.1051569837412103 1106 62-63 -0.10330170117764936 1106 64-65 0.05945271442210043 1106 66-67 0.35769932026583007 1106 68-69 0.20236662201162758 1106 70-71 0.09415536603223273 1106 72-73 0.21203787637271532 1106 74-75 0.1788435240273536 1106 76-77 0.5083608183041797 1106 78-79 0.603692423004393 1106 80-81 0.7203636180612136 1106 82-83 0.6883280276146237 1106 84-85 0.7460369987192159 1106 86-87 0.9737656816982962 1106 88-89 1.1255352781757502 1106 90-91 1.5728955346939806 1106 92-93 1.6859251199043577 1106 94-95 1.5273431318780446 1106 96-97 1.7458352803977206 1106 98-99 1.7541685572720418 1106 100-101 2.133064373265203 1107 1 -0.00849166247782307 1107 2 -0.00793505692946539 1107 3 -0.04583519501528599 1107 4 0.08025933633338411 1107 5 0.0012259986144300683 1107 6 0.03558747449119437 1107 7 0.06389462328949236 1107 8 0.06923500628656143 1107 9 0.08705183559982999 1107 10-11 0.1068136257916592 1107 12-13 0.15630063395418858 1107 14-15 0.33965636060071347 1107 16-17 0.07916002808202194 1107 18-19 0.3369455582861107 1107 20-21 0.33596369169112705 1107 22-23 0.2501493947245379 1107 24-25 0.2240784863371772 1107 26-27 0.3544439656714147 1107 28-29 0.33295228086709017 1107 30-31 0.478664211682748 1107 32-33 0.2881906703666459 1107 34-35 0.2987682334629227 1107 36-37 0.29877857066095714 1107 38-39 0.47178708753707355 1107 40-41 0.5323423025195666 1107 42-43 0.6504053378757249 1107 44-45 0.5032557817139747 1107 46-47 0.583536513209161 1107 48-49 0.8053484659374632 1107 50-51 0.6036886258440148 1107 52-53 0.6893124935328743 1107 54-55 0.9300801440822042 1107 56-57 0.7677080217488239 1107 58-59 0.5115017544802996 1107 60-61 1.0268526218266913 1107 62-63 0.8419006806457858 1107 64-65 0.9347219587262501 1107 66-67 1.0225238715833953 1107 68-69 0.9560391805206585 1107 70-71 1.0701768567972607 1107 72-73 1.1035805913587602 1107 74-75 0.9387127955085397 1107 76-77 1.0543360035784062 1107 78-79 1.2219672386012235 1107 80-81 1.408739162908553 1107 82-83 1.6833386022695969 1107 84-85 1.876962028422323 1107 86-87 1.8820557234045978 1107 88-89 1.8063779057074818 1107 90-91 1.9633354891201797 1107 92-93 2.3044762353823742 1107 94-95 2.296188491050465 1107 96-97 2.448928236318654 1107 98-99 2.56000883294476 1107 100-101 2.855288221464029 1108 1 0.10892524548269833 1108 2 -0.013039519265856114 1108 3 0.018419508519798455 1108 4 0.009048089958142214 1108 5 0.01692907192612836 1108 6 0.16902362981473118 1108 7 0.03056930731170837 1108 8 0.01576091007687097 1108 9 0.05641078735795446 1108 10-11 0.13587523119930012 1108 12-13 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-0.10744933732237882 2316 52-53 0.007970968218295127 2316 54-55 0.011034545251909833 2316 56-57 0.006453012241173894 2316 58-59 -0.06492912757183689 2316 60-61 0.16472033294571986 2316 62-63 -0.2883866700159601 2316 64-65 -0.5325497630292233 2316 66-67 -0.2930148824980776 2316 68-69 -0.46251550549969167 2316 70-71 -0.602929757646784 2316 72-73 -0.4149392459127341 2316 74-75 -0.6283604398947595 2316 76-77 -0.442423062778154 2316 78-79 -0.45874322132650036 2316 80-81 -0.9067064086488088 2316 82-83 -0.9948756266260759 2316 84-85 -1.2299781756701336 2316 86-87 -1.0155154703165685 2316 88-89 -1.4026418595937677 2316 90-91 -1.4936240333004456 2316 92-93 -1.5686871976739347 2316 94-95 -1.481118912793498 2316 96-97 -1.4138093601782877 2316 98-99 -1.6143174877091866 2316 100-101 -1.4853810260302716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4435.0 3 951.0 4 943.0 5 1054.0 6 1198.0 7 1441.0 8 1828.0 9 2216.0 10 2799.0 11 3394.0 12 4257.0 13 5169.0 14 5855.0 15 6943.0 16 8085.0 17 10167.0 18 9111.0 19 7598.0 20 7916.0 21 8487.0 22 9238.0 23 10357.0 24 11868.0 25 13308.0 26 15207.0 27 17732.0 28 20931.0 29 24735.0 30 29636.0 31 36254.0 32 45268.0 33 58349.0 34 77065.0 35 105615.0 36 148281.0 37 181011.0 38 110173.0 39 8113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23362357510499 18.510807098424326 14.599351889860749 23.656217436609932 2 15.149500874775882 19.14595443275666 41.42384321491478 24.28070147755268 3 19.323755354203424 23.077294824163175 29.64495331499532 27.953996506638077 4 12.736385431901764 15.87622501279625 36.7949118760041 34.592477679297886 5 14.052431324669074 36.60054399816958 34.44102117016807 14.906003506993276 6 32.98214528561827 35.28548030738727 17.31034170127303 14.422032705721433 7 29.27311138832887 31.880797677677204 20.243169899669507 18.602921034324417 8 26.045584848541587 35.71371556229164 19.36543068507352 18.875268904093254 9 25.819676170015672 16.954460792526092 20.290816276250826 36.93504676120741 10-11 25.087632680855425 26.18913877055069 27.363421621349953 21.359806927243934 12-13 25.637834167551144 23.37879401570675 28.056178878373707 22.9271929383684 14-15 22.521493585552328 25.06327729324449 25.957510966668128 26.457718154535048 16-17 22.36045653452732 28.376835099910036 26.63413682437925 22.62857154118339 18-19 22.180878395121635 27.0347082879074 28.9390751386425 21.845338178328465 20-21 22.72420715561303 26.174370343001417 29.11336904981834 21.988053451567215 22-23 22.501545791137136 25.924506270615684 28.973767123793852 22.60018081445333 24-25 22.25009619311735 26.10811761523837 29.0456903759094 22.59609581573488 26-27 22.19212882099835 26.34278458834641 28.902245607154487 22.562840983500745 28-29 22.142622033183407 26.430487502020256 29.028041122512132 22.39884934228421 30-31 22.26678992318806 26.251076687763504 28.813790670217195 22.668342718831244 32-33 22.026380923425943 26.299126696727786 28.870943978602764 22.803548401243507 34-35 22.545394519643448 26.465115820698422 28.572308480706404 22.417181178951726 36-37 22.503892085763834 26.19265331861895 28.663487308844392 22.63996728677282 38-39 22.17147180905409 26.389152985894427 28.795139651759772 22.64423555329171 40-41 22.473460392428077 26.26346068305716 28.672480233527857 22.5905986909869 42-43 22.270408904754422 26.192989880468236 28.65747348186086 22.879127732916483 44-45 22.362965444530936 26.447841638010033 28.549300909458168 22.639892008000867 46-47 22.46421726341514 26.755682126122753 28.093131192692194 22.686969417769916 48-49 22.53215341791288 26.623833220574838 28.061245356411295 22.782768005100987 50-51 22.557954015206725 26.788312572031398 27.931699332396153 22.722034080365727 52-53 22.663263093571977 26.793775009363113 27.78005558731348 22.762906309751436 54-55 22.566488319922424 26.71919675477577 27.886095901243962 22.82821902405784 56-57 22.521601298787015 27.074941311258684 27.83801808884042 22.56543930111388 58-59 22.5720587865945 26.694192206339114 28.00513543048135 22.728613576585037 60-61 22.536877631477843 26.69256851939782 28.123196015422895 22.647357833701438 62-63 22.378591572603725 26.856772482759368 28.143626457733482 22.621009486903425 64-65 22.3841059602649 26.64941052460478 27.983568080898635 22.98291543423169 66-67 22.281165802215554 26.863199996059485 27.820351789733966 23.035282411990995 68-69 22.392540433404324 26.820660686456655 27.990121423350402 22.796677456788615 70-71 22.33667581835717 26.809133564440607 27.840792108858814 23.013398508343403 72-73 22.4014727122615 26.705225034926176 27.778035030610898 23.115267222201425 74-75 22.47671868864993 26.93754750973842 27.671248128553533 22.914485673058117 76-77 22.461969885302445 26.892465315086955 27.605697779998057 23.039867019612537 78-79 22.545842034710923 26.79526723882689 27.698831096469647 22.960059629992546 80-81 22.361668188880646 26.94042872245359 27.681781414013628 23.016121674652133 82-83 22.4738377333621 26.939672369513758 27.66407876214035 22.9224111349838 84-85 22.602192545659975 26.76283803551534 27.55891395208867 23.076055466736015 86-87 22.435409922627855 26.772742228798514 27.680490673770112 23.111357174803516 88-89 22.48353919803899 26.667336625986344 27.601929876934385 23.247194299040284 90-91 22.414937971910025 26.781829789246363 27.708852204479896 23.09438003436372 92-93 22.52977568491464 26.504103084455565 27.794678304386807 23.171442926242992 94-95 22.357998499608648 26.601850022929487 27.754481594099317 23.28566988336255 96-97 22.349033260146594 26.582648880499043 27.810944709938635 23.25737314941573 98-99 22.37473379715725 26.459047743846238 27.54647065401537 23.619747804981138 100-101 22.724243786855187 25.837502025449993 28.070003491900415 23.3682506957944 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1268.0 1 730.5 2 169.5 3 146.0 4 128.5 5 113.0 6 160.0 7 304.5 8 502.5 9 636.5 10 631.5 11 626.5 12 704.0 13 760.0 14 817.5 15 882.5 16 956.5 17 1010.0 18 1094.5 19 1223.5 20 1365.5 21 1631.0 22 1996.5 23 2512.0 24 3205.0 25 4097.5 26 5235.0 27 6564.0 28 7903.0 29 9263.0 30 10805.0 31 12598.5 32 14429.5 33 15950.5 34 17841.5 35 19855.5 36 21990.0 37 24224.5 38 25997.0 39 27475.0 40 29077.5 41 31681.5 42 33624.0 43 35251.5 44 37894.5 45 39833.0 46 40670.5 47 41108.0 48 41342.0 49 41135.0 50 40812.5 51 39858.5 52 38190.0 53 36982.0 54 35738.5 55 33186.5 56 29590.0 57 26339.0 58 23554.5 59 20213.5 60 16550.0 61 13329.0 62 10473.0 63 8123.5 64 6415.0 65 4992.0 66 3660.0 67 2746.0 68 2182.0 69 1639.0 70 1142.5 71 731.5 72 479.5 73 357.5 74 267.0 75 201.0 76 135.5 77 93.5 78 65.0 79 40.0 80 22.0 81 17.0 82 18.5 83 11.5 84 6.5 85 7.0 86 7.5 87 4.0 88 1.5 89 3.0 90 4.0 91 2.0 92 1.5 93 2.0 94 1.0 95 0.0 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3520198861736815 2 0.29636534551046817 3 0.30138015394478596 4 0.2968569933961856 5 0.29636534551046817 6 0.22045491195569664 7 0.210621954241348 8 0.21838999083568342 9 0.2220281851899924 10-11 0.22035658237855313 12-13 0.2227164922299968 14-15 0.21716087112138982 16-17 0.20895035142990873 18-19 0.20231310497272337 20-21 0.20162479793271898 22-23 0.20998281198991534 24-25 0.20673793594418025 26-27 0.21480096126994616 28-29 0.22261816265285334 30-31 0.22635468658430583 32-33 0.22394561194429038 34-35 0.2235031288471447 36-37 0.20727874861846945 38-39 0.19656082470982944 40-41 0.19159518106408335 42-43 0.2034930598984452 44-45 0.20629545284703457 46-47 0.21465346690423093 48-49 0.22443725983000784 50-51 0.2202090880128379 52-53 0.23284443867577592 54-55 0.21676755281281587 56-57 0.18554791206975896 58-59 0.16602949100677689 60-61 0.1842696275668936 62-63 0.17689490928113213 64-65 0.18589206558976115 66-67 0.18613788953261984 68-69 0.18308967264117176 70-71 0.1843679571440371 72-73 0.19567585851553806 74-75 0.20300141201272776 76-77 0.2066396063670368 78-79 0.20280475285844082 80-81 0.19852741625269915 82-83 0.18495793460689802 84-85 0.17905815997828883 86-87 0.18623621910976335 88-89 0.1967574838641164 90-91 0.1931192895098074 92-93 0.1866295374183373 94-95 0.18874362332692224 96-97 0.19710163738411857 98-99 0.19857658104127088 100-101 0.1751741416811211 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1016988.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.81469467984205 #Duplication Level Percentage of deduplicated Percentage of total 1 82.14146909551135 54.06115709074761 2 10.620468099169168 13.979657306076426 3 3.0821603203106513 6.085543213067091 4 1.335324099454067 3.5153579161681776 5 0.7407680743166368 2.4376712319862 6 0.46874685982358844 1.8510258876854517 7 0.30265560654911994 1.3943430436720912 8 0.2113822340461917 1.1129645755594415 9 0.1634987592301604 0.9684558827339413 >10 0.871915939870856 10.074376288864494 >50 0.044271813600574465 1.968250609015732 >100 0.016591723721125367 1.754544580306668 >500 1.49474879294228E-4 0.05459892166396002 >1k 5.97899517176912E-4 0.7420534524527035 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2556 0.2513303991787514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2118 0.20826204438990434 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1491 0.1466093995209383 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1378 0.13549815730372433 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.832957714348646E-5 0.0 10-11 0.0 0.0 0.0 9.832957714348646E-5 0.0 12-13 0.0 0.0 0.0 1.9665915428697292E-4 0.0 14-15 0.0 0.0 0.0 1.9665915428697292E-4 0.0 16-17 0.0 0.0 0.0 1.9665915428697292E-4 0.0 18-19 0.0 0.0 0.0 1.9665915428697292E-4 0.0 20-21 0.0 0.0 0.0 1.9665915428697292E-4 0.0 22-23 0.0 0.0 0.0 2.4582394285871617E-4 0.0 24-25 0.0 0.0 0.0 2.949887314304594E-4 0.0 26-27 0.0 0.0 0.0 2.949887314304594E-4 0.0 28-29 0.0 0.0 0.0 5.899774628609186E-4 0.0 30-31 0.0 0.0 0.0 0.0012291197142935808 0.0 32-33 0.0 0.0 0.0 0.001917426754297986 0.0 34-35 0.0 0.0 0.0 0.002409074640015418 0.0 36-37 0.0 0.0 0.0 0.0029498873143045936 0.0 38-39 0.0 0.0 0.0 0.0037856887200242283 0.0 40-41 0.0 0.0 0.0 0.005850609840037444 0.0 42-43 0.0 0.0 0.0 0.008013860537194146 0.0 44-45 0.0 0.0 0.0 0.013520316857229386 0.0 46-47 0.0 0.0 0.0 0.024779053440158585 0.0 48-49 0.0 0.0 0.0 0.03323539707449842 9.832957714348646E-5 50-51 0.0 0.0 0.0 0.04552659421743423 9.832957714348646E-5 52-53 0.0 0.0 0.0 0.06145598571467903 9.832957714348646E-5 54-55 0.0 0.0 0.0 0.08107273635480458 9.832957714348646E-5 56-57 0.0 9.832957714348646E-5 0.0 0.10211526586351069 9.832957714348646E-5 58-59 0.0 9.832957714348646E-5 0.0 0.13048334886940652 9.832957714348646E-5 60-61 0.0 9.832957714348646E-5 0.0 0.1644562177724811 9.832957714348646E-5 62-63 0.0 9.832957714348646E-5 0.0 0.20418136693844963 1.9665915428697292E-4 64-65 0.0 9.832957714348646E-5 0.0 0.24085829921297006 1.9665915428697292E-4 66-67 0.0 9.832957714348646E-5 0.0 0.29926606803620104 2.4582394285871617E-4 68-69 0.0 9.832957714348646E-5 0.0 0.37473401849382687 2.949887314304594E-4 70-71 0.0 9.832957714348646E-5 0.0 0.42827447324845525 2.949887314304594E-4 72-73 0.0 9.832957714348646E-5 0.0 0.5213434180147651 2.949887314304594E-4 74-75 0.0 9.832957714348646E-5 0.0 0.5996629262095521 2.949887314304594E-4 76-77 0.0 9.832957714348646E-5 0.0 0.6752292062443215 2.949887314304594E-4 78-79 0.0 9.832957714348646E-5 0.0 0.7771969777421169 2.949887314304594E-4 80-81 0.0 9.832957714348646E-5 0.0 0.8785747717770515 2.949887314304594E-4 82-83 0.0 9.832957714348646E-5 0.0 0.9950953206920828 2.949887314304594E-4 84-85 0.0 9.832957714348646E-5 0.0 1.158568242693129 2.949887314304594E-4 86-87 0.0 9.832957714348646E-5 0.0 1.3815305588659847 2.949887314304594E-4 88-89 0.0 9.832957714348646E-5 0.0 1.6471679115191131 2.949887314304594E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1590 0.0 49.653362 1 GTATCAA 2675 0.0 46.048313 1 ATCAACG 3445 0.0 35.322617 3 TCAACGC 3545 0.0 34.193806 4 CAACGCA 3585 0.0 33.67803 5 TATCAAC 3630 0.0 33.522427 2 AACGCAG 3740 0.0 32.26319 6 GTGGTAT 605 0.0 28.299898 1 ACGCAGA 4340 0.0 27.365 7 CGCAGAG 4380 0.0 26.898172 8 GCAGAGT 4745 0.0 24.22599 9 TACATGG 3055 0.0 24.11696 2 GTACATG 3145 0.0 24.044415 1 CGCGTTA 50 0.001653649 23.751066 70-71 TGGTATC 710 0.0 22.762642 2 ACCGTCC 130 2.9388178E-4 21.925682 8 ACATGGG 3125 0.0 21.903553 3 CCTATAC 200 5.348138E-7 21.390188 3 CGTAAGA 135 3.7773588E-4 21.126112 2 GAGTACT 2955 0.0 21.055363 12-13 >>END_MODULE