FastQCFastQC Report
Thu 26 May 2016
SRR937748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016988
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.2764044413503404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.24002249780725043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.15722899385243486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.048.345341
GTATCAA28350.042.4170151
ATCAACG36750.032.445473
TCAACGC37750.031.5859874
TATCAAC38000.031.5031972
CAACGCA37950.031.2943485
AACGCAG38950.030.6083476
GTGGTAT7550.029.5885431
GTACATG29750.027.6396031
ACATGGG28650.027.0271743
ACGCAGA44700.026.6749657
TACATGG29750.026.3472122
CGCAGAG45150.026.3038868
TGGTATC9300.023.4970072
GTATAGG2252.7903297E-923.2371461
GCAGAGT51200.022.8245819
GGACCGA2503.8198777E-1022.7989486
CATGGGG21050.022.7932854
ACCGAGT2554.8930815E-1022.355218
TGTACCG1150.003720799220.6542685
Produced by FastQC (version 0.11.5)