##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937748_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1016988 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3301022234284 33.0 31.0 34.0 31.0 34.0 2 32.57067241698034 34.0 31.0 34.0 31.0 34.0 3 32.67765794679976 34.0 31.0 34.0 31.0 34.0 4 36.25617706403615 37.0 35.0 37.0 35.0 37.0 5 36.08879652463943 37.0 35.0 37.0 35.0 37.0 6 36.18459608176301 37.0 36.0 37.0 35.0 37.0 7 36.14861335630312 37.0 36.0 37.0 35.0 37.0 8 36.134984876911034 37.0 36.0 37.0 35.0 37.0 9 37.84852623629777 39.0 38.0 39.0 35.0 39.0 10-11 37.786835242893716 39.0 37.5 39.0 35.0 39.0 12-13 37.715586614591324 39.0 37.0 39.0 35.0 39.0 14-15 39.01441167447403 40.0 38.0 41.0 36.0 41.0 16-17 38.97279958072268 40.0 38.0 41.0 36.0 41.0 18-19 38.91728220981959 40.0 38.0 41.0 35.5 41.0 20-21 38.81753914500466 40.0 38.0 41.0 35.0 41.0 22-23 38.67435456465563 40.0 38.0 41.0 34.5 41.0 24-25 38.62458750742388 40.0 38.0 41.0 34.0 41.0 26-27 38.56128243401102 40.0 38.0 41.0 34.0 41.0 28-29 38.45714797028087 40.0 38.0 41.0 34.0 41.0 30-31 38.57618673966654 40.0 38.0 41.0 34.5 41.0 32-33 38.54213471545387 40.0 38.0 41.0 34.5 41.0 34-35 38.4399663516187 40.0 38.0 41.0 34.0 41.0 36-37 38.29696958076201 40.0 38.0 41.0 34.0 41.0 38-39 38.17300744944876 40.0 38.0 41.0 34.0 41.0 40-41 38.01056403812041 40.0 38.0 41.0 33.0 41.0 42-43 37.832779737814015 40.0 37.5 41.0 33.0 41.0 44-45 37.586257163309696 40.0 37.0 41.0 33.0 41.0 46-47 37.31118262949022 40.0 37.0 41.0 32.5 41.0 48-49 37.04535845064052 40.0 37.0 41.0 31.5 41.0 50-51 36.821964467624 39.5 36.0 41.0 31.0 41.0 52-53 36.600656054938696 39.0 36.0 41.0 31.0 41.0 54-55 36.38548389951504 39.0 35.5 40.5 30.5 41.0 56-57 36.11124320050974 39.0 35.0 40.0 30.0 41.0 58-59 35.840281301254294 38.5 35.0 40.0 29.0 41.0 60-61 35.49912929159439 38.0 35.0 40.0 28.5 41.0 62-63 35.12820013608813 38.0 34.0 40.0 28.0 41.0 64-65 34.72457836277321 37.0 34.0 40.0 28.0 41.0 66-67 34.300769527270724 36.5 34.0 39.0 26.5 41.0 68-69 33.837837319614394 36.0 33.0 39.0 26.0 40.0 70-71 33.43797812756886 35.5 33.0 38.5 26.0 40.0 72-73 32.98352438770172 35.0 32.5 38.0 25.5 40.0 74-75 32.488338112150785 35.0 32.0 37.0 24.5 39.0 76-77 31.26293476422534 34.0 30.5 36.0 23.0 38.5 78-79 31.82391434313876 34.5 31.5 36.0 24.0 38.5 80-81 31.628806829579112 34.5 31.5 36.0 24.0 37.5 82-83 31.27960064425539 34.0 31.0 35.0 23.0 37.0 84-85 30.898351799627918 34.0 31.0 35.0 21.0 36.5 86-87 30.487406931055233 34.0 31.0 35.0 19.5 36.0 88-89 30.07370047630847 34.0 30.0 35.0 18.0 36.0 90-91 29.574788001431678 34.0 30.0 35.0 12.5 35.0 92-93 29.13043025089775 34.0 29.0 35.0 4.5 35.0 94-95 28.661708889387093 34.0 29.0 35.0 2.0 35.0 96-97 28.143325191644344 33.5 29.0 35.0 2.0 35.0 98-99 27.34788021097594 33.0 27.0 35.0 2.0 35.0 100-101 24.923731646784425 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12313963097876979 1101 2 0.04943390744785603 1101 3 0.04135167672461648 1101 4 0.044558143692441377 1101 5 -0.08998269335493347 1101 6 -0.05413452504529204 1101 7 0.0060712341741790965 1101 8 -0.03450071473925931 1101 9 0.16323443965652018 1101 10-11 0.03144436346584456 1101 12-13 -0.003275937063783374 1101 14-15 0.003087045134797961 1101 16-17 -0.13631272435325315 1101 18-19 0.005572692494119735 1101 20-21 0.06700339616234885 1101 22-23 0.034470639287945914 1101 24-25 -0.07466591607495587 1101 26-27 0.011053566225477596 1101 28-29 -0.010641403571199248 1101 30-31 0.11108505905377086 1101 32-33 0.0664113216314206 1101 34-35 -0.039962163951784646 1101 36-37 0.07986297176153556 1101 38-39 0.13148406996555906 1101 40-41 0.14281296886203876 1101 42-43 0.1674019098194819 1101 44-45 0.2837409906561632 1101 46-47 0.11592562268520368 1101 48-49 0.17377501778940285 1101 50-51 0.30761203929858993 1101 52-53 0.2815153485617401 1101 54-55 0.13728692437226897 1101 56-57 0.35238372271276575 1101 58-59 0.32451571744434204 1101 60-61 0.2746669315960375 1101 62-63 0.2678171873153161 1101 64-65 0.051718501989107324 1101 66-67 0.12493473044992243 1101 68-69 0.23857389266162699 1101 70-71 0.1214014272817181 1101 72-73 0.09885042416645007 1101 74-75 0.1589905682669297 1101 76-77 -0.05071965993267824 1101 78-79 -0.12100643492693663 1101 80-81 0.13561321570417917 1101 82-83 0.046369159820777384 1101 84-85 0.20697170842307244 1101 86-87 0.32478830473111486 1101 88-89 0.4364971644992863 1101 90-91 0.47325127813848766 1101 92-93 0.4942963606062243 1101 94-95 0.6285698167311082 1101 96-97 0.9246832167849632 1101 98-99 1.0518736361523757 1101 100-101 1.6600218185487776 1102 1 -0.0044834853068493885 1102 2 0.08902125076507161 1102 3 0.04635339808355354 1102 4 -0.003755315769559786 1102 5 -0.09972988832230101 1102 6 -0.03984333370842563 1102 7 0.03144562974246412 1102 8 0.04482459080195156 1102 9 0.03192409428932308 1102 10-11 -0.013095798967846406 1102 12-13 0.07501557507179513 1102 14-15 0.002643012773248188 1102 16-17 0.11131160470797852 1102 18-19 0.13388337430874486 1102 20-21 0.015616919784093852 1102 22-23 0.13131175003391604 1102 24-25 0.02013010289129369 1102 26-27 0.0502721866345297 1102 28-29 -0.06401331099338137 1102 30-31 -0.054635838243697776 1102 32-33 -0.012784881989688301 1102 34-35 -0.004710493451177911 1102 36-37 0.07151340288370278 1102 38-39 -0.1465232223368318 1102 40-41 0.022339360424247445 1102 42-43 0.162259350002266 1102 44-45 0.17741186682786036 1102 46-47 0.19311879045510238 1102 48-49 0.14084033553661612 1102 50-51 0.23475845652470184 1102 52-53 0.05638409494297747 1102 54-55 0.10940873398996587 1102 56-57 0.23983912386764672 1102 58-59 0.2707330311854861 1102 60-61 0.5068939005959336 1102 62-63 0.4822329815659785 1102 64-65 0.4938436760810916 1102 66-67 0.533060131448309 1102 68-69 0.3868190845931494 1102 70-71 0.4446687230268367 1102 72-73 0.3455240936342392 1102 74-75 0.2906530097798452 1102 76-77 0.085936678580385 1102 78-79 0.31280535794664033 1102 80-81 0.3286479715059407 1102 82-83 0.40335846392230934 1102 84-85 0.37295667782982633 1102 86-87 0.43073305693367203 1102 88-89 0.678753005383129 1102 90-91 0.7070978894086899 1102 92-93 0.8629195081892789 1102 94-95 0.9055198425514916 1102 96-97 0.9334543229928087 1102 98-99 0.7101750852236215 1102 100-101 1.663229805654236 1103 1 0.07120585956664627 1103 2 0.14605641389277935 1103 3 0.08878134041399477 1103 4 0.0509252607698798 1103 5 -0.12490127294609721 1103 6 -0.012486209534600334 1103 7 0.03577221309824097 1103 8 0.028878305631991452 1103 9 0.2149274439022406 1103 10-11 0.10240094316270643 1103 12-13 0.0895508162790648 1103 14-15 0.11866923188790679 1103 16-17 0.031366192077044275 1103 18-19 -0.007927885934314816 1103 20-21 -0.04633627933048956 1103 22-23 0.03299080207004579 1103 24-25 0.031071976566373394 1103 26-27 0.13316803045980663 1103 28-29 0.09212139966330568 1103 30-31 0.05300732062087121 1103 32-33 0.030906973141505034 1103 34-35 0.02283328965943099 1103 36-37 0.11788363075888242 1103 38-39 -0.022485064858180692 1103 40-41 -0.03181222712830589 1103 42-43 0.11820686492522015 1103 44-45 0.1831487621505019 1103 46-47 0.46526556830705346 1103 48-49 0.4407234114026082 1103 50-51 0.5803271001657109 1103 52-53 0.5009136949023727 1103 54-55 0.5353014761049053 1103 56-57 0.6243977694215985 1103 58-59 0.5241050919782282 1103 60-61 0.3052656490704493 1103 62-63 0.5022040238109184 1103 64-65 0.4810283810770031 1103 66-67 0.45465288605597465 1103 68-69 0.37368244341946166 1103 70-71 0.33764117265575777 1103 72-73 0.3852253514958761 1103 74-75 0.386562519479277 1103 76-77 0.367445195107873 1103 78-79 0.16998857943100631 1103 80-81 0.22519695276294271 1103 82-83 0.4104722434022179 1103 84-85 0.5789268846235309 1103 86-87 0.7755462808775491 1103 88-89 0.7841091652744367 1103 90-91 0.7631892659122101 1103 92-93 0.9618199528523483 1103 94-95 1.0988314014488516 1103 96-97 1.1487713845468264 1103 98-99 1.376570181437156 1103 100-101 2.003624458766293 1104 1 0.050851004336777805 1104 2 0.04354397024553691 1104 3 -1.620406756970283E-4 1104 4 -0.006895000742225932 1104 5 -0.08669870828075688 1104 6 -0.13445842355776705 1104 7 0.04732506422605809 1104 8 -0.06167309993882242 1104 9 0.04438350578116257 1104 10-11 0.009261158053710972 1104 12-13 -0.027441668338241243 1104 14-15 -0.12004621538962823 1104 16-17 -0.15720003234504532 1104 18-19 -0.06958227924187099 1104 20-21 -0.07051038191205805 1104 22-23 -0.08657416852687305 1104 24-25 -0.17595827417343202 1104 26-27 -0.1949799663211209 1104 28-29 -0.22780231125556583 1104 30-31 -0.21004671495547456 1104 32-33 -0.3513530404310856 1104 34-35 -0.31625401643284334 1104 36-37 -0.30305524967867115 1104 38-39 -0.4429605093101898 1104 40-41 -0.3672442670287097 1104 42-43 -0.13906771774272642 1104 44-45 -0.2024824517894146 1104 46-47 -0.3045159835601865 1104 48-49 -0.2701805788897005 1104 50-51 -0.2115157201084159 1104 52-53 -0.26366839280483845 1104 54-55 -0.4384120916962573 1104 56-57 -0.3137631692570082 1104 58-59 -0.2108231473434472 1104 60-61 -0.319395855973859 1104 62-63 -0.2829710702259618 1104 64-65 -0.41295653770186647 1104 66-67 -0.3051608818022018 1104 68-69 -0.4915427027129837 1104 70-71 -0.3620835912744411 1104 72-73 -0.3691419713477089 1104 74-75 -0.19123443148026809 1104 76-77 -0.5383100390446067 1104 78-79 -0.5820885573065766 1104 80-81 -0.4065904166512908 1104 82-83 -0.3807921017957838 1104 84-85 -0.29640202374127256 1104 86-87 -0.014679937439399993 1104 88-89 -0.14620060775040855 1104 90-91 -0.23944528062942894 1104 92-93 0.10750232891412637 1104 94-95 0.2611555947246167 1104 96-97 0.30282588179320413 1104 98-99 0.422619118460819 1104 100-101 1.064979397248127 1105 1 0.02367184386392296 1105 2 0.015232226775587776 1105 3 0.020595374203608685 1105 4 -0.031268186839774614 1105 5 -0.21830202108602492 1105 6 -0.09769194801447867 1105 7 -0.0022480935569149096 1105 8 -0.11800211529948257 1105 9 0.05844452369013453 1105 10-11 0.04792895730337676 1105 12-13 0.015085407474025203 1105 14-15 -0.020288305005252028 1105 16-17 -0.03660684200031028 1105 18-19 0.02226737036526316 1105 20-21 0.00711544098027872 1105 22-23 0.13250985497421652 1105 24-25 0.11172063854689185 1105 26-27 0.05847653541314202 1105 28-29 0.014274562815359104 1105 30-31 0.022905787345081308 1105 32-33 0.028091993900332568 1105 34-35 0.006410385067823654 1105 36-37 0.07807025467469231 1105 38-39 0.045461661001972686 1105 40-41 0.03404546185923607 1105 42-43 -0.026477642001239587 1105 44-45 0.1879146601239441 1105 46-47 0.04755027254514488 1105 48-49 -0.09105691498371016 1105 50-51 0.07504901408850628 1105 52-53 0.30864420010235705 1105 54-55 0.001600649862460557 1105 56-57 0.23798596360913393 1105 58-59 0.17492187990933417 1105 60-61 0.3055492845372143 1105 62-63 0.3530832937578978 1105 64-65 0.3470406308406453 1105 66-67 0.3663773074807324 1105 68-69 0.44809770218544287 1105 70-71 0.41638742167947385 1105 72-73 0.2804750740263984 1105 74-75 0.34100737498961564 1105 76-77 0.12999462578160959 1105 78-79 0.19420364910667942 1105 80-81 0.21842834175459913 1105 82-83 0.2529517928740006 1105 84-85 0.31076722663035383 1105 86-87 0.3955586128543622 1105 88-89 0.45369604405110664 1105 90-91 0.33602438738218154 1105 92-93 0.6257949600460009 1105 94-95 0.6119731780756474 1105 96-97 0.7096411999782397 1105 98-99 0.8097727958162402 1105 100-101 1.0961282611258092 1106 1 -0.1952608937880953 1106 2 -0.09369802887097478 1106 3 -0.14820085314809006 1106 4 -0.06600633526370103 1106 5 -0.15627174061940963 1106 6 -0.19041967201547294 1106 7 -0.21812300048171096 1106 8 -0.23730715377056555 1106 9 -0.1496666701544953 1106 10-11 -0.039988640115339535 1106 12-13 -0.24010446594369483 1106 14-15 -0.1397372997930475 1106 16-17 -0.11549490355405112 1106 18-19 -0.0451736820050499 1106 20-21 -0.05424966202294712 1106 22-23 -0.2579096039485904 1106 24-25 -0.21659407974548373 1106 26-27 -0.3078575425218233 1106 28-29 -0.27421885987016736 1106 30-31 -0.1822915320890104 1106 32-33 -0.18561860426298438 1106 34-35 -0.551087753428078 1106 36-37 -0.23744724346379797 1106 38-39 -0.10968353631745487 1106 40-41 -0.15804686874389517 1106 42-43 -0.10707332331173802 1106 44-45 -0.019427112027948112 1106 46-47 -0.06959542117538575 1106 48-49 0.022504762851987437 1106 50-51 0.10071295054917329 1106 52-53 -0.03171817269485899 1106 54-55 -0.05280987384069391 1106 56-57 -0.12096663008657771 1106 58-59 -0.004264022299658166 1106 60-61 -0.02172795547271278 1106 62-63 0.01759557968492942 1106 64-65 -0.0033341519838785416 1106 66-67 -0.06448394841402916 1106 68-69 -0.3264617418453355 1106 70-71 -0.2693708309886773 1106 72-73 -0.00832611733622457 1106 74-75 -0.23222667812530062 1106 76-77 -0.2966323287976884 1106 78-79 0.04184326191382226 1106 80-81 0.08140178429617251 1106 82-83 0.009194036606935185 1106 84-85 -0.026527387417797144 1106 86-87 -0.07251783291381741 1106 88-89 0.03307554268600654 1106 90-91 0.10911448418039527 1106 92-93 0.18870387044560388 1106 94-95 0.01845121232732083 1106 96-97 0.4881569607129137 1106 98-99 0.6823367074568445 1106 100-101 1.2998314132727415 1107 1 0.026755990817505904 1107 2 0.0646012460595955 1107 3 0.04212481835121196 1107 4 -0.0022796665761788404 1107 5 -0.09681953213668493 1107 6 -0.11769810076459919 1107 7 -5.227328924135577E-4 1107 8 -0.03282085204273244 1107 9 0.17147183866236304 1107 10-11 -0.011064258480047329 1107 12-13 0.012905390958756868 1107 14-15 -0.06349421164537006 1107 16-17 -0.06855353975456069 1107 18-19 0.10460768221255279 1107 20-21 0.04017416736100188 1107 22-23 0.08581371987278885 1107 24-25 0.13604958399790945 1107 26-27 -0.13065803170849932 1107 28-29 -0.07412199812115006 1107 30-31 -0.0036394434639959172 1107 32-33 -0.0911557514405743 1107 34-35 0.01283612299305048 1107 36-37 -0.04811423068013454 1107 38-39 -0.04231897726489109 1107 40-41 -0.10688748935593395 1107 42-43 0.011852695914271294 1107 44-45 0.01580896235351048 1107 46-47 0.11524153402083215 1107 48-49 -0.16236145992306916 1107 50-51 -0.07991609334174399 1107 52-53 0.08192539697416379 1107 54-55 -0.09537608302722589 1107 56-57 0.024774066133375072 1107 58-59 0.020664986400475982 1107 60-61 -0.07868510285936026 1107 62-63 -0.09385117945335963 1107 64-65 -0.03906289727580514 1107 66-67 0.01169781718750329 1107 68-69 -0.07724029428482737 1107 70-71 0.032967787930026304 1107 72-73 -0.17217821850448445 1107 74-75 0.023913416018743305 1107 76-77 -0.036813230308805345 1107 78-79 0.0936071561742402 1107 80-81 0.07225810487372186 1107 82-83 0.08068288531097068 1107 84-85 0.26295161483650276 1107 86-87 0.15577590208219405 1107 88-89 0.27329546763557033 1107 90-91 0.4795618948707272 1107 92-93 0.47717592861840075 1107 94-95 0.5454651875962178 1107 96-97 0.9187854819684915 1107 98-99 1.0488336918509553 1107 100-101 1.6435348769513283 1108 1 -0.05664472874610027 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 9.0 5 12.0 6 26.0 7 74.0 8 166.0 9 349.0 10 575.0 11 942.0 12 1497.0 13 2240.0 14 2893.0 15 3913.0 16 5199.0 17 7465.0 18 6827.0 19 4513.0 20 4080.0 21 4069.0 22 4375.0 23 4737.0 24 5594.0 25 6727.0 26 8143.0 27 10356.0 28 13024.0 29 16407.0 30 20978.0 31 26595.0 32 35062.0 33 46781.0 34 65714.0 35 98283.0 36 157744.0 37 244847.0 38 190904.0 39 15864.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.725365185201944 19.36285769655665 14.854029210881237 23.05774790736017 2 15.767934331575201 19.866016118184284 40.161142511022746 24.20490703921777 3 19.648314434388606 23.26487628172604 29.8255239983166 27.26128528556876 4 13.510778888246469 16.515632436174272 36.27869748708933 33.69489118848993 5 14.253990202625289 36.96694443269738 34.287074290693475 14.491991073983849 6 33.43962760622544 35.71458070301715 16.71189827215267 14.133893418604742 7 29.517162444394625 32.18818707792029 19.921473999693212 18.373176477991873 8 26.457735981152187 35.95430821209297 18.96925037463569 18.61870543211916 9 26.247605674796553 16.926060091171184 20.057758793614084 36.768575440418175 10-11 25.341695280573617 26.49505205567814 26.933552804949517 21.22969985879873 12-13 25.824889287907492 23.53109501597686 27.886123019117658 22.757892676997994 14-15 22.63714026124202 25.40221713530543 25.67011606823286 26.290526535219687 16-17 22.554346757287206 28.646159050057623 26.459456748752196 22.340037443902975 18-19 22.470176639252383 27.202828351956953 28.7705951299327 21.55639987885796 20-21 22.870536756843126 26.433166582676776 29.060239186813995 21.6360574736661 22-23 22.777342668757157 26.13785925378557 28.840156678579316 22.24464139887796 24-25 22.6444342836915 26.332050128566305 28.844771555136017 22.178744032606186 26-27 22.46627376962375 26.720014395627032 28.69597153520414 22.117740299545073 28-29 22.314943510921943 26.847836187327704 28.82036920586863 22.016851095881723 30-31 22.507575075972003 26.597993188700148 28.661294913944285 22.233136821383567 32-33 22.319732386222846 26.63266429889045 28.693898059760787 22.353705255125924 34-35 22.76005223267138 26.77937202798853 28.414445401518996 22.046130337821097 36-37 22.82976790286611 26.534138062592678 28.445763371839195 22.190330662702017 38-39 22.542891361549987 26.721603401416733 28.589029565737256 22.146475671296024 40-41 22.82637553245466 26.607737751084574 28.32801370320987 22.237873013250894 42-43 22.61448237509103 26.640265402526676 28.37244270533957 22.37280951704273 44-45 22.70002202582528 26.893680161417834 28.208739926134825 22.197557886622064 46-47 22.83566767749472 27.18861972805972 27.76222531632625 22.21348727811931 48-49 22.950664117964024 27.077703965041866 27.72677750376602 22.244854413228083 50-51 23.060547420421873 27.19412618437976 27.554159931090634 22.191166464107738 52-53 23.193352917317075 27.307956095822224 27.287110215218966 22.211580771641735 54-55 22.91693707300381 27.22106848851707 27.495063855227396 22.366930583251722 56-57 22.913987185689507 27.54604774097629 27.499587015775994 22.0403780575582 58-59 22.944026871506843 27.173821126699625 27.53233076496478 22.349821236828753 60-61 22.91128312231806 27.162759049270985 27.785234437377827 22.14072339103313 62-63 22.7676727749 27.339358969820687 27.738429558657522 22.15453869662179 64-65 22.721802027162564 27.20681069983127 27.592262642233734 22.479124630772436 66-67 22.667671594945073 27.379133283775225 27.54939094659917 22.403804174680527 68-69 22.792058510031584 27.307500186826196 27.588329459147992 22.312111843994227 70-71 22.7812422565458 27.315022399477673 27.41305698788973 22.490678356086796 72-73 22.920832360279746 27.224621738221956 27.245860937327155 22.608684964171143 74-75 22.862670386804165 27.594931107806143 27.09015597536843 22.452242530021262 76-77 22.79132103820301 27.377166692232358 27.23670289128289 22.59480937828175 78-79 22.928638292683885 27.400569131592505 27.282819462963182 22.387973112760427 80-81 22.748706350864687 27.6532897405327 27.172212047837363 22.42579186076525 82-83 22.75746446604424 27.59652305589561 27.16288834151929 22.48312413654085 84-85 22.91211071167295 27.465918834514603 27.09929556413867 22.52267488967378 86-87 22.713689837048225 27.471071438404387 27.222936750482795 22.59230197406459 88-89 22.771999276294313 27.432427914587 27.107841980436348 22.687730828682344 90-91 22.76101368317902 27.48524813760964 27.188711242096737 22.56502693711461 92-93 22.94129273393965 27.10718503462768 27.375582741127737 22.57593949030494 94-95 22.708970017345337 27.355730844415078 27.260990296837328 22.674308841402258 96-97 22.713739001836796 27.29850303051757 27.25336975460871 22.73438821303693 98-99 22.76051269192761 27.335948907801 26.943760232451808 22.959778167819582 100-101 22.721057059561385 27.37726045462569 27.186083412785557 22.715599073027366 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 95.0 1 71.0 2 63.5 3 76.5 4 72.0 5 80.0 6 143.5 7 301.0 8 500.5 9 639.0 10 678.5 11 691.0 12 732.0 13 820.5 14 937.5 15 1033.5 16 1083.0 17 1150.5 18 1251.0 19 1379.0 20 1503.0 21 1664.5 22 2009.5 23 2503.0 24 3144.0 25 4014.5 26 5205.0 27 6526.0 28 7769.0 29 9339.0 30 11003.0 31 12745.5 32 14647.0 33 15951.5 34 17702.5 35 19816.5 36 21986.5 37 24244.0 38 26140.0 39 27508.0 40 28846.0 41 31558.0 42 33909.5 43 36032.5 44 38404.0 45 40163.0 46 40952.5 47 41215.5 48 41464.0 49 41144.0 50 41224.0 51 40519.0 52 38229.0 53 36716.0 54 35928.0 55 33416.5 56 29448.0 57 25971.5 58 23445.0 59 20335.0 60 16263.0 61 12758.5 62 10240.0 63 8010.5 64 6158.5 65 4674.5 66 3372.0 67 2700.5 68 2173.0 69 1499.0 70 970.0 71 630.0 72 432.5 73 304.5 74 246.5 75 190.5 76 106.5 77 62.5 78 44.5 79 27.5 80 12.5 81 5.5 82 5.0 83 4.5 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04414998013742542 2 0.0 3 0.0 4 0.0 5 0.018190971771544995 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0047689844914590935 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.916478857174323E-5 22-23 1.474943657152297E-4 24-25 2.949887314304594E-4 26-27 0.0012291197142935808 28-29 0.002114085908584959 30-31 1.474943657152297E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011553725314359659 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.916478857174323E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.916478857174323E-5 74-75 4.916478857174323E-5 76-77 0.0 78-79 0.0 80-81 4.916478857174323E-5 82-83 2.949887314304594E-4 84-85 3.9331830857394583E-4 86-87 0.0 88-89 0.0 90-91 0.008112190114337631 92-93 0.0026548985828741345 94-95 0.0 96-97 0.0 98-99 2.949887314304594E-4 100-101 0.012733680240081495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1016988.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.631312628433356 #Duplication Level Percentage of deduplicated Percentage of total 1 80.70545723074011 51.35394179869881 2 11.295594975744407 14.37507070451507 3 3.4231866009653453 6.534655703744701 4 1.4500027246014406 3.6906230672477767 5 0.8116220037702154 2.5822286729009045 6 0.5102630713488212 1.9481225409444858 7 0.3497390062824794 1.5578046432982646 8 0.24830844107135594 1.2640153633672297 9 0.18205345614200108 1.0425870344572605 >10 0.9565247406910102 10.754318336287698 >50 0.04700334174856137 2.0609694938310468 >100 0.019626257503253003 2.093436530419851 >500 1.5453734772297756E-4 0.068538897571536 >1k 4.636120431689327E-4 0.6736872127153417 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2811 0.2764044413503404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2441 0.24002249780725043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1599 0.15722899385243486 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.916478857174323E-5 0.0 24-25 0.0 0.0 0.0 9.832957714348646E-5 0.0 26-27 0.0 0.0 0.0 9.832957714348646E-5 0.0 28-29 0.0 0.0 0.0 2.949887314304594E-4 0.0 30-31 0.0 0.0 0.0 9.341309828631213E-4 0.0 32-33 0.0 0.0 0.0 0.0016224380228675264 0.0 34-35 0.0 0.0 0.0 0.002163250697156702 0.0 36-37 0.0 0.0 0.0 0.0027532281600176206 0.0 38-39 0.0 0.0 0.0 0.0035890295657372553 0.0 40-41 0.0 4.916478857174323E-5 0.0 0.005703115474322214 0.0 42-43 0.0 9.832957714348646E-5 0.0 0.007964695748622403 0.0 44-45 0.0 9.832957714348646E-5 0.0 0.013618646434372873 0.0 46-47 0.0 9.832957714348646E-5 0.0 0.025221536537304273 0.0 48-49 0.0 9.832957714348646E-5 0.0 0.03362871538307237 0.0 50-51 0.0 9.832957714348646E-5 0.0 0.04616573646886689 0.0 52-53 0.0 9.832957714348646E-5 0.0 0.0624392814861139 0.0 54-55 0.0 9.832957714348646E-5 0.0 0.08190853776052422 0.0 56-57 0.0 9.832957714348646E-5 0.0 0.10319689121208903 0.0 58-59 0.0 9.832957714348646E-5 0.0 0.13176163337227184 4.916478857174323E-5 60-61 0.0 9.832957714348646E-5 0.0 0.16593116142963338 9.832957714348646E-5 62-63 0.0 9.832957714348646E-5 0.0 0.20600046411560413 9.832957714348646E-5 64-65 0.0 9.832957714348646E-5 0.0 0.24243157244726582 9.832957714348646E-5 66-67 0.0 9.832957714348646E-5 0.0 0.30133098915621426 9.832957714348646E-5 68-69 0.0 9.832957714348646E-5 0.0 0.37709392834527056 9.832957714348646E-5 70-71 0.0 9.832957714348646E-5 0.0 0.43063438309989893 9.832957714348646E-5 72-73 0.0 9.832957714348646E-5 0.0 0.524047481386211 9.832957714348646E-5 74-75 0.0 9.832957714348646E-5 0.0 0.6035469445067199 9.832957714348646E-5 76-77 0.0 9.832957714348646E-5 0.0 0.6805881681986414 9.832957714348646E-5 78-79 0.0 9.832957714348646E-5 0.0 0.7833917411021566 9.832957714348646E-5 80-81 0.0 9.832957714348646E-5 0.0 0.8867361266799608 9.832957714348646E-5 82-83 0.0 9.832957714348646E-5 0.0 1.005419926292149 9.832957714348646E-5 84-85 0.0 9.832957714348646E-5 0.0 1.1709086046246366 9.832957714348646E-5 86-87 0.0 9.832957714348646E-5 0.0 1.3963291602260792 9.832957714348646E-5 88-89 0.0 9.832957714348646E-5 0.0 1.6661455199078061 9.832957714348646E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1750 0.0 48.34534 1 GTATCAA 2835 0.0 42.417015 1 ATCAACG 3675 0.0 32.44547 3 TCAACGC 3775 0.0 31.585987 4 TATCAAC 3800 0.0 31.503197 2 CAACGCA 3795 0.0 31.294348 5 AACGCAG 3895 0.0 30.608347 6 GTGGTAT 755 0.0 29.588543 1 GTACATG 2975 0.0 27.639603 1 ACATGGG 2865 0.0 27.027174 3 ACGCAGA 4470 0.0 26.674965 7 TACATGG 2975 0.0 26.347212 2 CGCAGAG 4515 0.0 26.303886 8 TGGTATC 930 0.0 23.497007 2 GTATAGG 225 2.7903297E-9 23.237146 1 GCAGAGT 5120 0.0 22.824581 9 GGACCGA 250 3.8198777E-10 22.798948 6 CATGGGG 2105 0.0 22.793285 4 ACCGAGT 255 4.8930815E-10 22.35521 8 TGTACCG 115 0.0037207992 20.654268 5 >>END_MODULE