FastQCFastQC Report
Thu 26 May 2016
SRR937747_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937747_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536203
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27060.5046596158544432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.44013181574888616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.27862581895289656No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9810.18295309798714293No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8220.15330014938372222No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7060.13166655166047186No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7030.1311070620641809No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.10331907878173005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC650.005800801329.2724254
GGTATCA24250.028.2622551
GTATCAA32750.025.286811
GTCCTAA1351.32049845E-524.6785931
TAAGGAT1852.501747E-723.1410394
GAGTACT22050.022.8349412-13
GTGTAGC1302.8994534E-421.9666581
GTACATG17100.021.709741
AGTACTT22750.021.29714212-13
GTACTTT23750.021.1024114-15
TATCAAC38950.020.885332
ATCAACG39100.020.6835423
TCAACGC39150.020.6551914
AACGCAG39650.020.6057226
CAACGCA39800.020.3178585
AGAGTAC32050.020.3065310-11
GTGCTAT1200.004705844419.831011
TACATGG18050.019.7668192
GTCTATG3153.110472E-1019.6421431
TGCTATA1707.8515906E-519.588532
Produced by FastQC (version 0.11.5)