##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937747_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 536203 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.618620186757628 33.0 31.0 34.0 30.0 34.0 2 31.763121429757014 33.0 31.0 34.0 30.0 34.0 3 31.783969877080136 33.0 31.0 34.0 30.0 34.0 4 35.40736810499009 37.0 35.0 37.0 33.0 37.0 5 35.380618907391415 37.0 35.0 37.0 33.0 37.0 6 35.44707694660418 37.0 35.0 37.0 33.0 37.0 7 35.37957825674231 37.0 35.0 37.0 33.0 37.0 8 35.3644216835788 37.0 35.0 37.0 33.0 37.0 9 36.98609668353217 39.0 37.0 39.0 33.0 39.0 10-11 36.86022737657193 39.0 37.0 39.0 33.0 39.0 12-13 36.74104117283939 39.0 37.0 39.0 32.0 39.0 14-15 37.87419316937802 40.0 38.0 41.0 33.0 41.0 16-17 37.772309181410776 40.0 37.0 41.0 33.0 41.0 18-19 37.680592984373455 40.0 37.0 41.0 32.0 41.0 20-21 37.570892926746026 40.0 37.0 41.0 32.0 41.0 22-23 37.46784053800519 40.0 37.0 41.0 32.0 41.0 24-25 37.480792722159336 40.0 37.0 41.0 32.0 41.0 26-27 37.36604233844272 40.0 37.0 41.0 32.0 41.0 28-29 37.2432567516407 39.5 37.0 41.0 31.5 41.0 30-31 36.97904058724028 39.0 36.0 41.0 31.0 41.0 32-33 36.814616852199634 39.0 36.0 41.0 31.0 41.0 34-35 36.786680604174165 39.0 36.0 41.0 30.5 41.0 36-37 36.62504032987506 39.0 35.0 41.0 30.0 41.0 38-39 36.44701540274859 39.0 35.0 41.0 30.0 41.0 40-41 36.18448236955034 39.0 35.0 41.0 30.0 41.0 42-43 35.89305263118632 39.0 35.0 40.0 29.0 41.0 44-45 35.638636859547596 38.5 35.0 40.0 27.5 41.0 46-47 35.23998280501974 38.0 35.0 40.0 26.5 41.0 48-49 34.87251376810649 38.0 34.0 40.0 25.5 41.0 50-51 33.92045736409531 37.5 33.0 39.5 23.5 40.5 52-53 33.96579560353075 37.5 33.0 39.5 24.0 40.5 54-55 34.204471067860496 38.0 34.0 40.0 23.5 41.0 56-57 33.91499301570487 37.5 33.0 40.0 22.5 41.0 58-59 33.62578445103813 37.0 33.0 40.0 22.0 41.0 60-61 33.25936911953122 36.5 33.0 40.0 21.0 41.0 62-63 32.8533680341214 36.0 32.0 39.0 19.5 41.0 64-65 32.429955632475014 35.5 32.0 39.0 18.0 40.5 66-67 31.962936611693706 35.0 31.0 38.5 15.5 40.0 68-69 31.446045620781682 35.0 31.0 38.0 10.5 40.0 70-71 30.96860330882147 35.0 30.0 37.0 7.5 39.5 72-73 30.521835013977913 34.5 30.0 36.5 7.0 39.0 74-75 30.018336339035777 34.0 29.0 36.0 2.0 39.0 76-77 29.558413511300756 34.0 29.0 35.5 2.0 38.0 78-79 29.027625731299526 34.0 29.0 35.0 2.0 37.0 80-81 28.493828829752914 34.0 27.5 35.0 2.0 36.5 82-83 27.999828423190472 33.0 26.5 35.0 2.0 36.0 84-85 27.531224181886337 33.0 26.0 35.0 2.0 36.0 86-87 27.014705251555846 33.0 25.5 35.0 2.0 35.0 88-89 26.55353849195174 32.0 25.0 35.0 2.0 35.0 90-91 26.009543960030065 32.0 23.5 34.5 2.0 35.0 92-93 25.37314505886763 32.0 20.5 34.0 2.0 35.0 94-95 24.626363522770294 31.0 17.5 34.0 2.0 35.0 96-97 23.76878626191946 31.0 4.5 34.0 2.0 35.0 98-99 22.69442822960707 31.0 2.0 34.0 2.0 35.0 100-101 19.941166871502023 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4522566279522948 1101 2 0.3772867618854221 1101 3 0.4159518320821185 1101 4 0.2926136833021076 1101 5 0.3331998884872931 1101 6 0.44074383284889507 1101 7 0.5731156740034749 1101 8 0.5454685269940356 1101 9 0.6903811856651672 1101 10-11 0.8201677565876295 1101 12-13 0.6770879194732871 1101 14-15 0.8814503629314316 1101 16-17 0.9563389836316816 1101 18-19 0.9314616464968068 1101 20-21 0.897459186073462 1101 22-23 0.8451210911854758 1101 24-25 1.1040926714809487 1101 26-27 0.8258730822321283 1101 28-29 0.9190394198448999 1101 30-31 0.7458600169931273 1101 32-33 0.9218152497547436 1101 34-35 0.983252085814236 1101 36-37 1.0108610188991705 1101 38-39 0.8190109619500348 1101 40-41 1.2418621960948784 1101 42-43 1.392103714425751 1101 44-45 1.5037433790319383 1101 46-47 1.5991235188288258 1101 48-49 1.4772624810793786 1101 50-51 1.4259714892526247 1101 52-53 1.335667708659173 1101 54-55 1.2924025916662814 1101 56-57 1.1514495097915827 1101 58-59 0.9962749896721093 1101 60-61 1.384646413671355 1101 62-63 1.2907377151799508 1101 64-65 1.5030298024660524 1101 66-67 1.54452398838637 1101 68-69 1.5223887425805458 1101 70-71 1.6240191038890117 1101 72-73 1.5345584252014497 1101 74-75 1.5721921216103816 1101 76-77 1.513631873062419 1101 78-79 2.000197066769104 1101 80-81 1.828783938862312 1101 82-83 1.7106523030732994 1101 84-85 1.3498088833566015 1101 86-87 1.6085184181448788 1101 88-89 1.6045361858481648 1101 90-91 1.8568722283103192 1101 92-93 1.7647233368963349 1101 94-95 1.6538392063911687 1101 96-97 2.2733932892936437 1101 98-99 2.52744290574066 1101 100-101 2.46795962703327 1102 1 0.45890526976363333 1102 2 0.5084966407710496 1102 3 0.5972755521592781 1102 4 0.31047252022460015 1102 5 0.26995478207740575 1102 6 0.2205164666913504 1102 7 0.16875018053352875 1102 8 0.34256241435450363 1102 9 0.5352093170885013 1102 10-11 0.6000352580896973 1102 12-13 0.5607959800621032 1102 14-15 0.6308021005907349 1102 16-17 0.7357321468356801 1102 18-19 0.627300118495036 1102 20-21 1.033701003737825 1102 22-23 0.9651365862365537 1102 24-25 0.7770996600652111 1102 26-27 0.48948026687622104 1102 28-29 0.7405814936519377 1102 30-31 0.3792083710392333 1102 32-33 0.35683485573773055 1102 34-35 0.28665467253908616 1102 36-37 0.7108697151013033 1102 38-39 0.5990681987713415 1102 40-41 0.7805879653134866 1102 42-43 0.6335142384114576 1102 44-45 0.29298301474012334 1102 46-47 0.255196630171838 1102 48-49 0.17929897350578017 1102 50-51 0.044366328451936 1102 52-53 0.05422303231600267 1102 54-55 0.2044286486501221 1102 56-57 0.03154074085757941 1102 58-59 0.09860557184331498 1102 60-61 -0.14434356197927656 1102 62-63 -0.007150688829788976 1102 64-65 0.3691359593771608 1102 66-67 0.4355052362123182 1102 68-69 -0.026645978982870133 1102 70-71 0.11210135414989253 1102 72-73 0.24980919204836383 1102 74-75 0.47881862763447813 1102 76-77 0.42378429376886473 1102 78-79 0.45726154515509165 1102 80-81 0.31956596460307196 1102 82-83 0.39012215096881775 1102 84-85 0.371470332301989 1102 86-87 0.4563427864843739 1102 88-89 0.5562762168382669 1102 90-91 0.7745033828497334 1102 92-93 0.7211118832075591 1102 94-95 0.7878358227779749 1102 96-97 0.7840619481811615 1102 98-99 1.0596900043714044 1102 100-101 1.5592065043061432 1103 1 0.20923840119912995 1103 2 0.27351784004421376 1103 3 0.40027373507871644 1103 4 0.17676567588783598 1103 5 0.16650232901865536 1103 6 0.10896286281491285 1103 7 0.28904091342268856 1103 8 0.395530312597991 1103 9 0.41918872350885295 1103 10-11 0.4816676021236219 1103 12-13 0.5377266281541608 1103 14-15 0.5812109437161084 1103 16-17 0.6206655205485774 1103 18-19 0.5781329470837662 1103 20-21 0.34162044649978185 1103 22-23 0.31210054747216276 1103 24-25 0.25053999925358283 1103 26-27 0.3688488313825786 1103 28-29 0.31203061539633126 1103 30-31 0.4257256333045234 1103 32-33 0.3583112336904861 1103 34-35 0.48937272220594963 1103 36-37 0.6107258628905186 1103 38-39 0.5978416693952013 1103 40-41 0.6927851185539495 1103 42-43 0.44518140982273025 1103 44-45 0.2067322576232229 1103 46-47 0.2391760365921911 1103 48-49 0.2330239886481209 1103 50-51 -0.06918606403745997 1103 52-53 0.14308584273393166 1103 54-55 0.49832628644539767 1103 56-57 0.031237894098040897 1103 58-59 0.08793779473852936 1103 60-61 0.47734026600376467 1103 62-63 0.17146832994671257 1103 64-65 0.24641488124269983 1103 66-67 0.10252522409844644 1103 68-69 0.13334796408194194 1103 70-71 0.29840510944971044 1103 72-73 0.490451227178589 1103 74-75 0.3220878584037088 1103 76-77 0.2772594603345837 1103 78-79 0.37003410723867347 1103 80-81 0.20954930803129912 1103 82-83 0.3296436530887483 1103 84-85 0.2588643359361491 1103 86-87 0.3991501759453122 1103 88-89 0.6808143755299731 1103 90-91 0.5629194942973434 1103 92-93 0.28772302796831184 1103 94-95 0.5050980880717368 1103 96-97 0.885553468471894 1103 98-99 1.2463647469516559 1103 100-101 1.293685607879734 1104 1 0.4031162462005682 1104 2 0.4169059126457668 1104 3 0.3114664749231473 1104 4 0.20845683117449454 1104 5 0.28139717200338055 1104 6 0.1393359541430641 1104 7 0.3991388679026642 1104 8 0.42810017719032345 1104 9 0.6012832207718901 1104 10-11 0.28256791903518064 1104 12-13 0.3630903561864329 1104 14-15 0.4331628307803541 1104 16-17 0.3890247830089635 1104 18-19 0.2560859865536642 1104 20-21 0.4531877181794144 1104 22-23 0.2422827716150664 1104 24-25 0.21700499493205427 1104 26-27 0.379627425447687 1104 28-29 0.5604928741113966 1104 30-31 0.6107803725727408 1104 32-33 0.6483393235781278 1104 34-35 0.3655791468674323 1104 36-37 0.3563215125592052 1104 38-39 0.5814991168166941 1104 40-41 0.8456481263326836 1104 42-43 0.7786047748472171 1104 44-45 0.7413116295621478 1104 46-47 0.9561019945293836 1104 48-49 1.0298548731194543 1104 50-51 1.056346923574175 1104 52-53 0.8880908845086353 1104 54-55 0.8529143014266651 1104 56-57 0.9861140104910149 1104 58-59 0.9598371032951434 1104 60-61 1.0265623921921758 1104 62-63 1.0602539824964126 1104 64-65 1.3040315623144352 1104 66-67 1.3734125764464729 1104 68-69 1.0679417410914454 1104 70-71 1.1197210126476378 1104 72-73 1.1612449465678125 1104 74-75 1.2484815489827916 1104 76-77 1.0495621108675728 1104 78-79 1.1479115200879981 1104 80-81 1.3606981124104394 1104 82-83 1.6331512877761547 1104 84-85 1.8088247219920426 1104 86-87 1.8010300376566306 1104 88-89 2.160794928684684 1104 90-91 2.114295178538196 1104 92-93 2.1810966954992814 1104 94-95 2.559743723335348 1104 96-97 2.861803108345132 1104 98-99 2.4457453289165088 1104 100-101 2.9531137265775165 1105 1 0.09750306158444744 1105 2 0.2500612235345798 1105 3 0.2143997370475006 1105 4 0.18667989329748025 1105 5 0.14320040242604648 1105 6 0.09249893369101869 1105 7 0.17796652163531945 1105 8 0.4056625021634517 1105 9 0.3367179036522927 1105 10-11 0.28825950199705375 1105 12-13 0.24569140104124187 1105 14-15 0.3523577585100881 1105 16-17 0.40490472741118566 1105 18-19 0.319087102617452 1105 20-21 0.4285983680298102 1105 22-23 0.49254809352757434 1105 24-25 0.31707072629618693 1105 26-27 0.562163765990924 1105 28-29 0.25520492280738694 1105 30-31 0.4751666401952548 1105 32-33 0.43040903990620905 1105 34-35 0.40816930496505677 1105 36-37 0.3904983983988899 1105 38-39 0.6318418100224008 1105 40-41 0.9032811809924226 1105 42-43 0.8491769800660123 1105 44-45 0.5463856115818757 1105 46-47 0.6408002807210025 1105 48-49 0.9216120915872281 1105 50-51 0.9316541834663994 1105 52-53 1.0294116056364757 1105 54-55 0.9442586447634937 1105 56-57 0.9631919090045216 1105 58-59 1.0677032988870323 1105 60-61 0.993491299613666 1105 62-63 1.3456667549220995 1105 64-65 1.0722463395467372 1105 66-67 1.3573768624052995 1105 68-69 1.4985181229906743 1105 70-71 1.5505901045277568 1105 72-73 1.4884929934561875 1105 74-75 1.4486540938136372 1105 76-77 1.6358465085697134 1105 78-79 1.4544473402579428 1105 80-81 1.3871104808621233 1105 82-83 1.57735494545269 1105 84-85 1.4438119523051576 1105 86-87 1.6690383773234565 1105 88-89 1.6357451166353023 1105 90-91 1.5088237271759795 1105 92-93 1.7836307765268806 1105 94-95 1.941945929444259 1105 96-97 2.0825458042896408 1105 98-99 2.5048094101495195 1105 100-101 2.5862077566633097 1106 1 0.33580202449172347 1106 2 0.25209440366750613 1106 3 0.16925288676815242 1106 4 0.33629119709559774 1106 5 0.3792269875377059 1106 6 0.4335674638213831 1106 7 0.4649934655434649 1106 8 0.4583987525692308 1106 9 0.4076502619703888 1106 10-11 0.2860349637339752 1106 12-13 0.17413029458119667 1106 14-15 0.37935975751913276 1106 16-17 0.228016347291927 1106 18-19 0.31042985578254445 1106 20-21 0.40359025239482804 1106 22-23 0.5886372117424798 1106 24-25 0.43163273829024007 1106 26-27 0.5305760060770339 1106 28-29 0.6262739655041685 1106 30-31 0.4750965894511765 1106 32-33 0.5035215140643174 1106 34-35 0.8091876035862597 1106 36-37 0.5843868981169749 1106 38-39 0.5234886859300687 1106 40-41 0.8437107116524416 1106 42-43 0.8660387067314232 1106 44-45 0.930294443101296 1106 46-47 0.7341796311284909 1106 48-49 0.5542676982095855 1106 50-51 0.5432330588956731 1106 52-53 0.7181846924823105 1106 54-55 0.7396814266323162 1106 56-57 0.5993363843927924 1106 58-59 0.41102370415621436 1106 60-61 0.5988715384696093 1106 62-63 0.8154834269991866 1106 64-65 0.9119702371017979 1106 66-67 0.7381470788791766 1106 68-69 0.5837613357569893 1106 70-71 0.6419619031233275 1106 72-73 0.8982693436415623 1106 74-75 0.6003139547372811 1106 76-77 0.9549823215854794 1106 78-79 1.0288856528892865 1106 80-81 0.8105108464928357 1106 82-83 0.6076271182217461 1106 84-85 0.7296497421187524 1106 86-87 0.93313651670735 1106 88-89 1.152929760145355 1106 90-91 1.3150582433987061 1106 92-93 1.405336255861915 1106 94-95 2.1230168515512453 1106 96-97 2.0176976093777164 1106 98-99 2.0959693479581247 1106 100-101 2.2561047308128757 1107 1 0.3051700868420042 1107 2 0.21928290530386363 1107 3 0.33369500694764653 1107 4 0.24063682279147258 1107 5 0.39110660560848487 1107 6 0.3726882168832262 1107 7 0.2125760425534864 1107 8 0.3472242864941322 1107 9 0.4101773377826632 1107 10-11 0.21866426971595843 1107 12-13 0.11422274034521962 1107 14-15 0.15934896093354922 1107 16-17 0.24319398486404253 1107 18-19 0.17211378573969682 1107 20-21 0.17756879418098492 1107 22-23 0.340644701873714 1107 24-25 0.1773242258572978 1107 26-27 0.14163069753086432 1107 28-29 0.28947785227497747 1107 30-31 0.2816892545208489 1107 32-33 0.19788704596485474 1107 34-35 0.35224067555224536 1107 36-37 0.3117359989638402 1107 38-39 0.33021462135299373 1107 40-41 0.2044381316058761 1107 42-43 0.267852195715534 1107 44-45 0.23249593437968485 1107 46-47 0.24298053534253228 1107 48-49 0.2640014948963838 1107 50-51 -0.3494623761729869 1107 52-53 -0.38356300480842265 1107 54-55 0.1316295355428707 1107 56-57 -0.09605313617132794 1107 58-59 -0.30785851184297286 1107 60-61 0.009789585637193454 1107 62-63 0.10259162903030017 1107 64-65 0.11259441470562592 1107 66-67 -0.07575858595431484 1107 68-69 0.16951152975813955 1107 70-71 0.24332665901927442 1107 72-73 0.27344900557347884 1107 74-75 0.02484750850090478 1107 76-77 -0.09214140053739683 1107 78-79 0.40228556516702696 1107 80-81 0.1404747454098043 1107 82-83 0.1735689516169323 1107 84-85 0.21916355837436186 1107 86-87 -0.02832135973922334 1107 88-89 0.021521817907089513 1107 90-91 0.013509608154603114 1107 92-93 0.34059722957897876 1107 94-95 0.7023835643283327 1107 96-97 1.0857582726929458 1107 98-99 0.9906857688900068 1107 100-101 0.8800157939391653 1108 1 0.007551491617480366 1108 2 -0.09058472405834905 1108 3 -0.0885926616824122 1108 4 -0.1282275764738401 1108 5 -0.13579186801239018 1108 6 -0.0292625083051945 1108 7 0.06994509695844897 1108 8 -0.07093392491888295 1108 9 -0.06778833452083433 1108 10-11 0.0584894644717977 1108 12-13 0.29138722194633004 1108 14-15 0.218694074620295 1108 16-17 0.2082836042192966 1108 18-19 0.315143743305093 1108 20-21 0.034332997107071606 1108 22-23 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1108 88-89 1.1108244969874583 1108 90-91 1.2225348789127253 1108 92-93 1.1554018403839663 1108 94-95 0.9792883714725455 1108 96-97 0.8592208099023928 1108 98-99 0.7471744635508486 1108 100-101 1.007686957407401 1109 1 0.13327513645668887 1109 2 -0.03193720760113905 1109 3 0.16076952295332347 1109 4 0.1869162075823496 1109 5 -0.07803571821239075 1109 6 0.03075198034012061 1109 7 0.08915830052391982 1109 8 0.23927427061483542 1109 9 0.33260537866697604 1109 10-11 0.2038641219413293 1109 12-13 0.2039277744046828 1109 14-15 0.24905723566472915 1109 16-17 0.2037307049153796 1109 18-19 0.39827864519175904 1109 20-21 0.27850761678013924 1109 22-23 0.22054378735616353 1109 24-25 0.10568431633685549 1109 26-27 0.028500467896868997 1109 28-29 0.37545935452076407 1109 30-31 0.4571197777597078 1109 32-33 0.35483277767612265 1109 34-35 0.4133386799465981 1109 36-37 0.5149293568830586 1109 38-39 0.2416097953446581 1109 40-41 0.4312138576783795 1109 42-43 0.5692233239229907 1109 44-45 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-0.6686781357044858 2316 96-97 -0.7932052727868459 2316 98-99 -1.0445443439574333 2316 100-101 -1.6171535529594259 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1961.0 3 345.0 4 329.0 5 426.0 6 475.0 7 615.0 8 723.0 9 939.0 10 1184.0 11 1647.0 12 2245.0 13 2790.0 14 3220.0 15 3942.0 16 4899.0 17 6011.0 18 5425.0 19 4165.0 20 4344.0 21 4586.0 22 4967.0 23 5546.0 24 6161.0 25 7041.0 26 8080.0 27 9636.0 28 11149.0 29 13747.0 30 16596.0 31 20069.0 32 25431.0 33 32430.0 34 43117.0 35 58874.0 36 78654.0 37 89898.0 38 50815.0 39 3721.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.480124350314526 18.265309369133647 14.701561116757158 23.55300516379467 2 16.260614326042912 19.924345742203066 38.42577782267645 25.389262109077578 3 19.202399013755418 22.49879806118383 30.706964510534974 27.591838414525782 4 13.64644873555305 16.05580104089108 35.27131637925551 35.02643384430036 5 14.747390459155705 35.803330707733295 33.756111068494484 15.693167764616517 6 33.609352895775984 34.258568846978264 16.386816094724978 15.745262162520767 7 28.622739496143808 32.65796196317413 20.222270810090578 18.497027730591483 8 26.561229810112746 33.326605706896004 19.740537873733665 20.371626609257586 9 26.12932824287682 16.184437480261398 20.602146438455744 37.084087838406035 10-11 25.65179810401297 25.249987151393956 26.79882820712887 22.2993865374642 12-13 25.90901786081347 23.019482824990938 27.629975819321373 23.441523494874218 14-15 23.120247186035147 25.14419029662137 25.886701417470263 25.84886109987321 16-17 22.671501868330147 27.943939817250307 26.672596983574202 22.71196133084534 18-19 22.392759271180694 26.145612499170777 29.125583142339522 22.33604508730901 20-21 22.922657518530766 25.36916726925528 29.263758172316052 22.444417039897903 22-23 22.85132382892057 24.999532876174815 29.40796726396233 22.74117603094228 24-25 23.06079605810815 25.39302601301071 29.15151973795463 22.39465819092651 26-27 22.608214714136768 25.524677401875234 29.36063349938152 22.506474384606474 28-29 22.211850057747725 25.869766729086543 29.37705340823718 22.54132980492855 30-31 22.504737839545168 25.40313687964206 29.225274458278978 22.8668508225338 32-33 22.560549946872484 25.69605454611721 29.028649607921746 22.714745899088562 34-35 23.139835549155364 25.613980658008966 28.551070126632194 22.695113666203472 36-37 23.034123520267848 25.47642891306947 28.63702095539878 22.8524266112639 38-39 22.749084332915164 25.433079471776153 28.76133432847811 23.056501866830573 40-41 22.886171684588817 25.59331803154336 28.754847201631446 22.76566308223638 42-43 22.942748234238948 25.839530625210212 28.21508651294892 23.00263462760193 44-45 22.85767142179085 25.66440309301083 28.21867840685276 23.25924707834556 46-47 23.12633732935911 25.93152873681358 27.854666753875335 23.08746717995197 48-49 23.192886756012033 25.92538606223528 27.544665674355674 23.337061507397014 50-51 22.827697822231443 26.417567918607933 27.5434553877953 23.211278871365323 52-53 23.326020651859967 26.32462510433891 27.135968204323863 23.213386039477264 54-55 22.992522058246475 26.051994304656056 27.200966414661398 23.754517222436068 56-57 23.14341094322879 26.3413745592116 27.215338268606526 23.299876228953085 58-59 23.48796499723505 25.90701549866236 27.249230297867257 23.355789206235333 60-61 23.028036405481743 25.950674756773722 28.035004408709817 22.98628442903472 62-63 22.66418935897328 26.052458765250808 27.980032077958594 23.303319797817316 64-65 22.64883935335201 26.254405375842893 27.638251505977674 23.458503764827423 66-67 22.761723106583545 26.320454931821875 27.321035926387427 23.59678603520715 68-69 22.505128559631416 25.979937896786105 27.76465796524163 23.75027557834085 70-71 22.888646847052186 26.15453021983668 27.452495137886 23.504327795225134 72-73 22.791528346263995 26.10056959672403 27.48336885918127 23.624533197830715 74-75 22.54469602777744 26.72579118905119 27.09057093401363 23.63894184915774 76-77 22.7373062246323 26.438550030125967 26.99324150751281 23.830902237728925 78-79 22.89352582774528 26.374904478353724 27.09227437740439 23.63929531649661 80-81 23.02364314184484 26.534104655301093 26.94090755812704 23.50134464472702 82-83 22.80050179767241 26.344284391869234 27.119305852780485 23.735907957677867 84-85 22.8409706525191 26.279445554912108 26.904597336122993 23.974986456445798 86-87 22.935278541840816 26.107419220764665 26.92485511341082 24.03244712398369 88-89 22.829046488841314 26.105150284746603 26.590485157402245 24.475318069009834 90-91 22.99653065151637 25.817594846606966 26.86447121860632 24.321403283270342 92-93 22.953070534303233 25.542391941925676 27.090455321549594 24.4140822022215 94-95 22.856966714678833 25.430007790300795 27.23999170529124 24.473033789729133 96-97 22.758712808676425 25.554956710340853 27.209014934909053 24.477315546073665 98-99 22.634699327729365 25.70452038249462 26.77714560072337 24.88363468905265 100-101 22.848716234137374 24.813029298925247 27.344044768369262 24.994209698568113 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1129.0 1 701.0 2 298.0 3 319.5 4 277.5 5 262.5 6 403.5 7 758.0 8 1135.5 9 1274.0 10 1055.5 11 845.5 12 868.5 13 899.0 14 937.0 15 992.0 16 1044.5 17 1066.0 18 1066.5 19 1088.0 20 1158.0 21 1297.5 22 1455.0 23 1696.5 24 1947.0 25 2281.0 26 2740.5 27 3210.5 28 3614.5 29 4100.0 30 4842.0 31 5623.5 32 6419.0 33 6623.0 34 6818.5 35 7352.0 36 7772.0 37 8463.5 38 9742.0 39 10714.0 40 11329.0 41 13083.5 42 14962.0 43 16132.5 44 17125.0 45 18249.5 46 18756.5 47 19878.5 48 22649.5 49 23096.0 50 21544.5 51 20518.5 52 20207.5 53 20892.5 54 20948.0 55 19722.0 56 17934.0 57 16492.5 58 15623.5 59 14241.0 60 12182.5 61 10032.0 62 8377.5 63 6832.0 64 5262.0 65 4044.5 66 2982.5 67 2484.5 68 1885.0 69 1165.5 70 936.5 71 748.5 72 589.0 73 452.5 74 323.0 75 270.0 76 188.5 77 105.0 78 72.5 79 47.0 80 28.5 81 17.5 82 9.0 83 9.0 84 8.0 85 5.5 86 9.0 87 9.0 88 5.0 89 2.0 90 2.5 91 2.0 92 0.0 93 0.5 94 0.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35546239017685466 2 0.3107032224735781 3 0.30771927796002635 4 0.30977073981309317 5 0.32114702827100927 6 0.20440019917829627 7 0.20328121998571436 8 0.20440019917829627 9 0.20216224079313244 10-11 0.20990184687515734 12-13 0.1986188066832897 14-15 0.19759307575675633 16-17 0.2050529370406357 18-19 0.19833906188514425 20-21 0.1887344904821495 22-23 0.18892098701424648 24-25 0.19181168326174974 26-27 0.18966697314263442 28-29 0.208316626352333 30-31 0.21428451537943652 32-33 0.2181076942874247 34-35 0.214377763645485 36-37 0.18108813266617307 38-39 0.17484049884092406 40-41 0.18118138093222155 42-43 0.19078595233521634 44-45 0.1978728205549018 46-47 0.2029082269215204 48-49 0.2030014751875689 50-51 0.20775713675604204 52-53 0.24011428507486904 54-55 0.19190493152779825 56-57 0.17539998843721502 58-59 0.17362827138229364 60-61 0.1676603823551901 62-63 0.17614597456560294 64-65 0.1714835612631783 66-67 0.17045783033664488 68-69 0.17996915347359116 70-71 0.17567973323536049 72-73 0.17008483727245094 74-75 0.1768919606939909 76-77 0.17726495375818488 78-79 0.1835125875834339 80-81 0.17372151964834215 82-83 0.17297553351995418 84-85 0.1665414031626082 86-87 0.16234523119042601 88-89 0.16831312021752956 90-91 0.17605272629955446 92-93 0.16579541703422024 94-95 0.17194980259342077 96-97 0.1703645820705964 98-99 0.17297553351995418 100-101 0.15385963898001317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 536203.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.31587133906658 #Duplication Level Percentage of deduplicated Percentage of total 1 69.06865994750667 33.37112487585473 2 15.177150800417913 14.665945307332063 3 6.244743833183664 9.05160718768594 4 3.191462890771514 6.167932416556878 5 1.8468882546199696 4.461700764392584 6 1.1490794771081065 3.331126570459031 7 0.7305445336172085 2.4707826985595207 8 0.5071686100597022 1.960343464868624 9 0.362434154070616 1.5760189761263383 >10 1.569302893553762 13.505364990080611 >50 0.09926355337765062 3.2926347633976505 >100 0.04982489616674202 4.121391885815491 >500 0.002317437031011257 0.7998318144715959 >1k 0.0011587185155056284 1.2241942843988962 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2706 0.5046596158544432 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2360 0.44013181574888616 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.27862581895289656 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 981 0.18295309798714293 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 822 0.15330014938372222 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 706 0.13166655166047186 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 703 0.1311070620641809 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 554 0.10331907878173005 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.8649653209698567E-4 0.0 22-23 0.0 0.0 0.0 3.7299306419397133E-4 0.0 24-25 0.0 0.0 0.0 3.7299306419397133E-4 0.0 26-27 0.0 0.0 0.0 4.662413302424642E-4 0.0 28-29 0.0 0.0 0.0 0.0028906962475032773 0.0 30-31 0.0 0.0 0.0 0.007459861283879426 0.0 32-33 0.0 0.0 0.0 0.01286826071469201 0.0 34-35 0.0 0.0 0.0 0.020514618530668424 0.0 36-37 0.0 0.0 0.0 0.027881231548499356 0.0 38-39 0.0 0.0 0.0 0.03431536190584536 0.0 40-41 0.0 0.0 0.0 0.039910257868754934 0.0 42-43 0.0 0.0 0.0 0.04522540903351902 0.0 44-45 0.0 0.0 0.0 0.05156629112481653 0.0 46-47 0.0 0.0 0.0 0.06322232438087813 0.0 48-49 0.0 0.0 0.0 0.07618383336161864 0.0 50-51 0.0 0.0 0.0 0.08672088742509833 0.0 52-53 0.0 0.0 0.0 0.1090072230106881 0.0 54-55 0.0 0.0 0.0 0.1584288040163893 0.0 56-57 0.0 0.0 0.0 0.23591811310268684 0.0 58-59 0.0 0.0 0.0 0.3135006704550329 0.0 60-61 0.0 0.0 0.0 0.3805461737438992 0.0 62-63 0.0 0.0 0.0 0.45673000710551787 0.0 64-65 0.0 0.0 0.0 0.5595828445570055 0.0 66-67 0.0 0.0 0.0 0.721648330949286 0.0 68-69 0.0 0.0 0.0 0.9112220558258719 0.0 70-71 0.0 0.0 0.0 1.0782297003187225 0.0 72-73 0.0 0.0 0.0 1.2707873697088603 0.0 74-75 0.0 0.0 0.0 1.4857246229506362 0.0 76-77 0.0 0.0 0.0 1.7187520398058198 0.0 78-79 0.0 0.0 0.0 1.9487955121474516 0.0 80-81 0.0 0.0 0.0 2.2024307957993523 0.0 82-83 0.0 0.0 0.0 2.442731577406318 0.0 84-85 0.0 0.0 0.0 2.811435221362059 0.0 86-87 0.0 0.0 0.0 3.246997872074569 0.0 88-89 0.0 0.0 0.0 3.7531494601857878 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGCGC 65 0.0058008013 29.272425 4 GGTATCA 2425 0.0 28.262255 1 GTATCAA 3275 0.0 25.28681 1 GTCCTAA 135 1.32049845E-5 24.678593 1 TAAGGAT 185 2.501747E-7 23.141039 4 GAGTACT 2205 0.0 22.83494 12-13 GTGTAGC 130 2.8994534E-4 21.966658 1 GTACATG 1710 0.0 21.70974 1 AGTACTT 2275 0.0 21.297142 12-13 GTACTTT 2375 0.0 21.10241 14-15 TATCAAC 3895 0.0 20.88533 2 ATCAACG 3910 0.0 20.683542 3 TCAACGC 3915 0.0 20.655191 4 AACGCAG 3965 0.0 20.605722 6 CAACGCA 3980 0.0 20.317858 5 AGAGTAC 3205 0.0 20.30653 10-11 GTGCTAT 120 0.0047058444 19.83101 1 TACATGG 1805 0.0 19.766819 2 GTCTATG 315 3.110472E-10 19.642143 1 TGCTATA 170 7.8515906E-5 19.58853 2 >>END_MODULE