FastQCFastQC Report
Thu 26 May 2016
SRR937747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937747_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536203
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28750.5361775297788337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26760.49906471989153367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18030.33625324737086515No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11480.21409801884733956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9760.182020615326658No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.1284961106148231No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6400.11935778054207082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.10947346434093058No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.10723550595576675No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG5530.10313258224963306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG804.4860138E-429.6925435
ATATAAG908.927569E-426.3958321
GCCTACA1457.8563244E-726.2137931
GTATCAA39450.023.8465771
GTTCTAA1401.773568E-523.756251
GTAAGGT1401.7748493E-523.7540343
GGTATCA30050.023.5586091
GTACATG15800.021.6512641
GAGTACT26450.021.28036312-13
TATCAAC45500.020.36062
AACCTAT1404.84764E-420.3568047
AACGCAG45600.020.3102686
AGTACTT27100.020.1564912-13
TCAACGC46000.020.139294
CAACGCA46300.020.0087975
ATCAACG46650.019.8586793
GTACTTT28050.019.81065614-15
CTAGCCT1200.00475556919.7950294
TCGAACG600.00476632519.78949252-53
AGAGTAC35250.019.53868110-11
Produced by FastQC (version 0.11.5)