##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937747_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 536203 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.030357905494746 31.0 31.0 34.0 30.0 34.0 2 32.307385449167576 33.0 31.0 34.0 31.0 34.0 3 32.393682989464814 34.0 31.0 34.0 31.0 34.0 4 36.077252458490534 37.0 35.0 37.0 35.0 37.0 5 35.96158171438802 37.0 35.0 37.0 35.0 37.0 6 35.996137656820274 37.0 35.0 37.0 35.0 37.0 7 35.92832005788852 37.0 35.0 37.0 35.0 37.0 8 35.90553018166627 37.0 35.0 37.0 35.0 37.0 9 37.57301059486799 39.0 37.0 39.0 35.0 39.0 10-11 37.48338315899016 39.0 37.0 39.0 35.0 39.0 12-13 37.37834085225185 39.0 37.0 39.0 34.0 39.0 14-15 38.57661184290278 40.0 38.0 41.0 34.0 41.0 16-17 38.522824378080685 40.0 38.0 41.0 34.0 41.0 18-19 38.483777599155545 40.0 38.0 41.0 34.0 41.0 20-21 38.380440803203264 40.0 38.0 41.0 34.0 41.0 22-23 38.31272484488151 40.0 38.0 41.0 34.0 41.0 24-25 38.2990192147377 40.0 38.0 41.0 34.0 41.0 26-27 38.13642874060757 40.0 37.5 41.0 33.5 41.0 28-29 37.95758602618784 40.0 37.0 41.0 33.0 41.0 30-31 37.99211492662294 40.0 37.0 41.0 33.0 41.0 32-33 37.90424708552544 40.0 37.0 41.0 33.0 41.0 34-35 37.73573907643187 40.0 37.0 41.0 33.0 41.0 36-37 37.56449516321244 40.0 36.5 41.0 33.0 41.0 38-39 37.41084160290039 40.0 36.0 41.0 32.5 41.0 40-41 37.201028341877986 40.0 36.0 41.0 32.0 41.0 42-43 36.93786308543592 39.0 36.0 41.0 31.0 41.0 44-45 36.634046247410026 39.0 35.0 41.0 31.0 41.0 46-47 36.23281014839529 39.0 35.0 41.0 30.0 41.0 48-49 35.86629317627839 39.0 35.0 40.0 29.0 41.0 50-51 35.56333515478279 39.0 35.0 40.0 28.0 41.0 52-53 35.273700445540214 38.0 35.0 40.0 27.5 41.0 54-55 34.985488704837536 38.0 34.0 40.0 27.0 41.0 56-57 34.65576097112474 38.0 34.0 40.0 26.0 41.0 58-59 34.35329716543921 37.0 34.0 40.0 26.0 41.0 60-61 33.975163324337984 37.0 33.0 40.0 25.5 41.0 62-63 33.56660443899045 36.0 33.0 39.0 24.5 41.0 64-65 33.136611507209025 36.0 32.5 39.0 23.0 40.0 66-67 32.673329876930936 35.0 32.0 38.5 22.0 40.0 68-69 32.21288485890605 35.0 31.5 38.0 21.0 40.0 70-71 31.80743766819656 35.0 31.0 37.0 20.0 39.5 72-73 31.33133813126745 35.0 31.0 36.5 18.5 39.0 74-75 30.88072521041471 34.0 30.5 36.0 17.5 39.0 76-77 29.662785363006176 33.5 29.0 35.0 15.5 37.0 78-79 30.280278178227277 34.0 30.0 35.0 15.5 37.0 80-81 30.136631089344895 34.0 30.0 35.0 12.0 37.0 82-83 29.78336376335082 34.0 30.0 35.0 7.5 36.0 84-85 29.451846595412558 34.0 30.0 35.0 4.5 36.0 86-87 29.10026333310332 34.0 29.0 35.0 2.0 35.5 88-89 28.685576171711084 34.0 29.0 35.0 2.0 35.0 90-91 28.1619871578488 33.0 28.0 35.0 2.0 35.0 92-93 27.7483658241375 33.0 27.0 35.0 2.0 35.0 94-95 27.285991499488066 33.0 26.5 35.0 2.0 35.0 96-97 26.72115691258721 33.0 25.0 34.5 2.0 35.0 98-99 25.84310606244277 32.0 24.5 34.0 2.0 35.0 100-101 23.19088945791053 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4495753637435769 1101 2 0.3584766219262008 1101 3 0.4266146335803356 1101 4 0.12729061243643258 1101 5 0.12877803684639844 1101 6 0.23573616262579833 1101 7 0.2985628451929685 1101 8 0.13087085316849567 1101 9 0.2830912611592993 1101 10-11 0.42257617071634 1101 12-13 0.46018721061548007 1101 14-15 0.525834317169334 1101 16-17 0.5496291114371701 1101 18-19 0.4837227234232131 1101 20-21 0.5551615202318558 1101 22-23 0.3308137638498678 1101 24-25 0.32950590205532393 1101 26-27 0.3549918366930527 1101 28-29 0.29828491802580714 1101 30-31 0.1464487696893002 1101 32-33 0.3199364674685157 1101 34-35 0.5683801432000948 1101 36-37 0.34912792699816464 1101 38-39 0.5190028307550634 1101 40-41 0.6666902854341359 1101 42-43 0.675818206924049 1101 44-45 0.452445732325657 1101 46-47 0.5363895613473844 1101 48-49 0.6256876768601884 1101 50-51 0.7274452665798279 1101 52-53 0.5752978598026104 1101 54-55 0.7112889163637561 1101 56-57 0.604241741720152 1101 58-59 0.16753905070546438 1101 60-61 0.44080461256985615 1101 62-63 0.27961072054602454 1101 64-65 0.07116254953486845 1101 66-67 -0.01853872551165381 1101 68-69 0.3291350963159303 1101 70-71 0.14735552360074067 1101 72-73 -0.08461958334269681 1101 74-75 0.06780841368045287 1101 76-77 0.18634288994141457 1101 78-79 0.08112042013161513 1101 80-81 0.016316626250404198 1101 82-83 0.152836334241087 1101 84-85 0.2297810350173144 1101 86-87 0.12513897679354002 1101 88-89 -0.21588462343077452 1101 90-91 -0.1837986007818806 1101 92-93 -0.49133872304089365 1101 94-95 -0.5449780143995504 1101 96-97 -0.588184070512451 1101 98-99 -0.3426963608698408 1101 100-101 -0.38420701821340586 1102 1 0.30024576187152263 1102 2 0.19783405164507428 1102 3 0.2115891774250045 1102 4 0.13920440935639533 1102 5 0.1193624216296243 1102 6 0.1544582952507909 1102 7 0.059923247431839854 1102 8 0.21866296650372874 1102 9 0.1974731983569029 1102 10-11 0.3110671424773983 1102 12-13 0.4578550473166203 1102 14-15 0.45864034442797674 1102 16-17 0.4482630171387143 1102 18-19 0.46563462298998104 1102 20-21 0.40085531904917815 1102 22-23 0.44420828682365254 1102 24-25 0.35319514722498013 1102 26-27 0.26012812989737455 1102 28-29 0.34641444400373445 1102 30-31 0.49228486999604115 1102 32-33 0.4161401799562654 1102 34-35 0.2580798870574128 1102 36-37 0.28189047324615046 1102 38-39 0.3788714390464918 1102 40-41 0.751723331002232 1102 42-43 0.7722274660076209 1102 44-45 0.46306695738336856 1102 46-47 0.22843332696102436 1102 48-49 0.2820256270862842 1102 50-51 0.08901358710603091 1102 52-53 0.4197741903215402 1102 54-55 0.4872154571726597 1102 56-57 0.22222944054696114 1102 58-59 0.21166832370297328 1102 60-61 0.10616289609767193 1102 62-63 0.053280201619934076 1102 64-65 -0.11885737267365215 1102 66-67 0.057861363031499025 1102 68-69 0.1344429418713844 1102 70-71 -0.06126636553058873 1102 72-73 -0.31731809392044497 1102 74-75 -0.3793937809247403 1102 76-77 -0.531639274938577 1102 78-79 -0.3223556890294397 1102 80-81 -0.06716296139150302 1102 82-83 -0.0787751510702357 1102 84-85 -0.006514540987492268 1102 86-87 -0.364919683042654 1102 88-89 -0.427593664318735 1102 90-91 -0.05263615572606639 1102 92-93 -0.2995123624805416 1102 94-95 -0.9077954257773442 1102 96-97 -0.7748322379854891 1102 98-99 -0.5954751927327244 1102 100-101 -0.3822819952870553 1103 1 0.2847854347970227 1103 2 0.3305263593373837 1103 3 0.2148599224280332 1103 4 0.09981918827826064 1103 5 0.08508016844973554 1103 6 0.1029743461290451 1103 7 0.17374820200483043 1103 8 0.10417174905975912 1103 9 0.29330905541323204 1103 10-11 0.1223406131012581 1103 12-13 0.10805946887931128 1103 14-15 0.2450728096006003 1103 16-17 0.2300392133834137 1103 18-19 0.3235010675690262 1103 20-21 0.3125603463053821 1103 22-23 0.19029399245660272 1103 24-25 2.272489814885148E-4 1103 26-27 0.1089924545490959 1103 28-29 0.2663417607814438 1103 30-31 0.2293457421947096 1103 32-33 0.4051619849229482 1103 34-35 0.4214732849980578 1103 36-37 0.5238498917803796 1103 38-39 0.4666742858060289 1103 40-41 0.24117669260126462 1103 42-43 0.2955239529852136 1103 44-45 0.6189270421804594 1103 46-47 0.6297128545200792 1103 48-49 0.5013093944999767 1103 50-51 0.20953902623383414 1103 52-53 0.34777237324097854 1103 54-55 0.24758795868689987 1103 56-57 0.3797476113525349 1103 58-59 0.2823830420554785 1103 60-61 0.3458131080904039 1103 62-63 0.4897126667925562 1103 64-65 0.3325735782651762 1103 66-67 0.11162423401876964 1103 68-69 0.015696727455875248 1103 70-71 0.19551740188310518 1103 72-73 0.2241037716155958 1103 74-75 0.34705231235206213 1103 76-77 0.22823352942241826 1103 78-79 0.20761099782700754 1103 80-81 0.18420742019541336 1103 82-83 0.15331903427198057 1103 84-85 -0.09134191833455318 1103 86-87 0.14554367249944278 1103 88-89 0.02256533023002305 1103 90-91 0.2754074542073077 1103 92-93 0.1630557780403521 1103 94-95 -0.01744866623767294 1103 96-97 -0.2535648242968165 1103 98-99 -0.06559178873514426 1103 100-101 0.26550722336830646 1104 1 0.36625331148961493 1104 2 0.26337712280612635 1104 3 0.17822782793077607 1104 4 0.014908499715886592 1104 5 0.08417264900288757 1104 6 0.24090289697894463 1104 7 0.28540910664326447 1104 8 0.18996114692780708 1104 9 0.14717681686526873 1104 10-11 0.148366974380437 1104 12-13 0.1647470228983181 1104 14-15 0.3983355580880641 1104 16-17 0.4090330192030649 1104 18-19 0.3107273712279124 1104 20-21 0.17633159378448937 1104 22-23 0.4281469166859324 1104 24-25 0.3748671222168696 1104 26-27 0.3228069172399657 1104 28-29 0.17060566167455704 1104 30-31 0.2995668600281789 1104 32-33 0.26251577345652066 1104 34-35 0.4967001648597673 1104 36-37 0.43440160598377275 1104 38-39 0.44208843158533995 1104 40-41 0.20902817632353532 1104 42-43 0.15861456087918668 1104 44-45 0.43376426488284636 1104 46-47 0.6635611691268224 1104 48-49 0.547430829245009 1104 50-51 0.20785003170775695 1104 52-53 0.5951074351827828 1104 54-55 0.2892186126770966 1104 56-57 0.43752995073599266 1104 58-59 0.45855705530814816 1104 60-61 0.4713424944343956 1104 62-63 0.41853721303282754 1104 64-65 0.29286960246569294 1104 66-67 0.3159565589971649 1104 68-69 0.28371747075751585 1104 70-71 -0.05007464651793825 1104 72-73 -0.26768893941868654 1104 74-75 -0.19407056290002345 1104 76-77 -0.10100876386481872 1104 78-79 0.001013475499149763 1104 80-81 -0.23998949711948114 1104 82-83 -0.07051775627829215 1104 84-85 -0.19781699756244464 1104 86-87 -0.3614139765930382 1104 88-89 -0.3036627020373359 1104 90-91 -0.23722579269560384 1104 92-93 0.10231679791935022 1104 94-95 0.15985254378825786 1104 96-97 0.2862687971379252 1104 98-99 0.7322438494094818 1104 100-101 0.5205129853965396 1105 1 0.02944511697955221 1105 2 0.03238615402167255 1105 3 -0.09503320090201584 1105 4 -0.07297296472412995 1105 5 -0.0409428944106196 1105 6 0.019617574929498005 1105 7 0.10492173596630039 1105 8 -0.13780898501421035 1105 9 0.1101104898106513 1105 10-11 0.06184806634271922 1105 12-13 -0.07373458259024801 1105 14-15 0.016585255107557373 1105 16-17 -0.056167016401431624 1105 18-19 0.12910681922139133 1105 20-21 -0.03167393860532286 1105 22-23 -0.14146841789356301 1105 24-25 -0.013606951552894486 1105 26-27 -0.2372106111238068 1105 28-29 -0.06825252975434637 1105 30-31 0.08896288465005853 1105 32-33 0.1500466706211654 1105 34-35 -0.1095895050455411 1105 36-37 -0.1168089466389759 1105 38-39 -0.155613367126044 1105 40-41 -0.014636484167120045 1105 42-43 -0.008858342050643842 1105 44-45 -0.4687821407755237 1105 46-47 -0.6407853173263618 1105 48-49 -0.6922164809043281 1105 50-51 -0.4047012353327588 1105 52-53 -0.14066103978110078 1105 54-55 -0.2501620061563088 1105 56-57 -0.3911970518453316 1105 58-59 -0.32945073659730895 1105 60-61 -0.3691408365024813 1105 62-63 -0.7130436262745263 1105 64-65 -0.3244454767200651 1105 66-67 -0.43576888931127655 1105 68-69 -0.17438905513447622 1105 70-71 -0.13674247717750987 1105 72-73 0.04740394036823048 1105 74-75 -0.37903930626697857 1105 76-77 -0.42938143331137724 1105 78-79 -0.39224837497569 1105 80-81 -0.008084451552580418 1105 82-83 0.09556520218085751 1105 84-85 0.23803158750147446 1105 86-87 -0.1481773231411161 1105 88-89 -0.08752291333628293 1105 90-91 0.17660278538435747 1105 92-93 0.13031425082899162 1105 94-95 -0.16522499868036533 1105 96-97 -0.11726015547386837 1105 98-99 -0.034683688608581065 1105 100-101 0.16733186125327038 1106 1 0.18045401854971743 1106 2 0.15390881800021106 1106 3 0.10425604032882774 1106 4 0.11568906606402152 1106 5 0.04298814832033315 1106 6 -0.026824359837888778 1106 7 0.08127875556341735 1106 8 0.17856067788793695 1106 9 0.2280861941623371 1106 10-11 0.14062781655201206 1106 12-13 -0.02043621767712267 1106 14-15 0.21705339910455024 1106 16-17 0.34649320331871536 1106 18-19 0.32045469805069615 1106 20-21 0.36780836931040994 1106 22-23 0.37723683932935614 1106 24-25 0.412826098729866 1106 26-27 0.39105571838806696 1106 28-29 0.5628910059288188 1106 30-31 0.3438946278884458 1106 32-33 0.4734402020329398 1106 34-35 0.13643554236685418 1106 36-37 0.38580172499388965 1106 38-39 0.4928514964458586 1106 40-41 0.3821633928169348 1106 42-43 0.18275436276235268 1106 44-45 0.3466768624536414 1106 46-47 0.28520350872568656 1106 48-49 0.3157863894676254 1106 50-51 0.2577327717406632 1106 52-53 0.29525619782761225 1106 54-55 -0.01136243671209769 1106 56-57 0.13353086081335164 1106 58-59 0.3691192030907118 1106 60-61 0.30782784568924626 1106 62-63 0.06987082638996611 1106 64-65 -0.2575504332373484 1106 66-67 -0.36825534901501555 1106 68-69 0.14531570660756898 1106 70-71 0.16618598563579567 1106 72-73 0.29476336902753175 1106 74-75 0.18124533895163353 1106 76-77 0.23340966170135147 1106 78-79 0.3723845420398071 1106 80-81 0.3012545086210068 1106 82-83 0.2897766187435842 1106 84-85 -0.07309964658761103 1106 86-87 0.3680095244045454 1106 88-89 0.7816325480589228 1106 90-91 0.4077582809506559 1106 92-93 0.09502917847169812 1106 94-95 0.07389748760848036 1106 96-97 0.35438808727758797 1106 98-99 0.004924027224593175 1106 100-101 -0.8695934236482294 1107 1 0.22897454893197988 1107 2 0.11480156067034386 1107 3 0.031119294824591748 1107 4 0.0751107739489072 1107 5 -0.05422835695987516 1107 6 0.060972124523935634 1107 7 0.061230845714909776 1107 8 0.04016869435272952 1107 9 0.03658175744045167 1107 10-11 0.11712331235146678 1107 12-13 0.1106663836252082 1107 14-15 0.13891479793717565 1107 16-17 0.039581701581141715 1107 18-19 0.023872492173303783 1107 20-21 0.05593718607210718 1107 22-23 0.09468513380622312 1107 24-25 0.009634358117835973 1107 26-27 0.10757618129166957 1107 28-29 -0.01720447637489997 1107 30-31 0.034778260939496874 1107 32-33 -0.14652504645722075 1107 34-35 -0.08241799520188664 1107 36-37 0.11126311033077485 1107 38-39 -0.11627836623507193 1107 40-41 0.10441954180981838 1107 42-43 0.09597174526513186 1107 44-45 -0.19409711777510807 1107 46-47 -0.5204225245412957 1107 48-49 -0.49967297150946877 1107 50-51 -0.34044361747624663 1107 52-53 -0.08793992889731328 1107 54-55 -0.3564803556702216 1107 56-57 -0.4944019304414127 1107 58-59 -0.2769754694690931 1107 60-61 -0.5207274362028471 1107 62-63 -0.6188821679755634 1107 64-65 -0.8614523867445456 1107 66-67 -0.567739137266976 1107 68-69 -0.29461985127225176 1107 70-71 -0.2784342782057614 1107 72-73 -0.20678695318307305 1107 74-75 -0.6157670433824549 1107 76-77 -0.5996059596084073 1107 78-79 -0.5149244090044256 1107 80-81 -0.1979121299295521 1107 82-83 -0.30309932174023757 1107 84-85 -0.5173555256658524 1107 86-87 -0.7164113399970837 1107 88-89 -0.565108232669175 1107 90-91 -0.4857283414050251 1107 92-93 -0.7072094260696176 1107 94-95 -0.8585934871207606 1107 96-97 -0.8005968987205883 1107 98-99 -0.2927057848473389 1107 100-101 -0.1611450954320297 1108 1 0.01626316759054447 1108 2 0.15222001655735795 1108 3 0.041917952117643154 1108 4 -0.14643587245048906 1108 5 -0.01269311090248948 1108 6 0.019101201729583295 1108 7 -0.10853924738788123 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-0.548188891823191 2316 72-73 -0.3557039206920969 2316 74-75 -0.4647483869486351 2316 76-77 -0.527186050997166 2316 78-79 -0.5112701210541104 2316 80-81 -0.7863345378465461 2316 82-83 -0.7116285181755728 2316 84-85 -0.867390869383506 2316 86-87 -1.058652131696359 2316 88-89 -0.8061934110287226 2316 90-91 -0.7380540842542338 2316 92-93 -0.35162953664496044 2316 94-95 -0.42837523966424484 2316 96-97 -0.33896692219891733 2316 98-99 -0.4433015789449364 2316 100-101 0.07757016043123954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 11.0 6 32.0 7 87.0 8 183.0 9 394.0 10 583.0 11 956.0 12 1286.0 13 1852.0 14 2378.0 15 3131.0 16 4300.0 17 5536.0 18 5137.0 19 3518.0 20 3344.0 21 3377.0 22 3454.0 23 3641.0 24 4313.0 25 4930.0 26 6038.0 27 7298.0 28 9139.0 29 11194.0 30 14062.0 31 17517.0 32 22410.0 33 29478.0 34 40198.0 35 58013.0 36 84647.0 37 112016.0 38 67701.0 39 4044.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94150453157554 18.97498167411826 15.071428971120648 23.012084823185553 2 16.81583280958891 20.49242544334888 37.469577753201676 25.222163993860537 3 19.474340874631434 22.584357043880768 30.951337459879934 26.98996462160786 4 14.152475834711852 16.68528523712102 34.742811957411654 34.41942697075548 5 14.65408194867853 36.40581797557436 33.472016477796394 15.468083597950724 6 33.73069527772131 34.60331255140311 15.99450208223378 15.671490088641802 7 28.934004472186842 33.01585407019356 19.766394443895315 18.28374701372428 8 27.047405553493732 33.613761952096496 19.15375333595672 20.18507915845305 9 26.225142343478126 16.19685081955901 20.21417261746018 37.36383421950269 10-11 25.98092513469712 25.539581091489605 26.353731702358996 22.12576207145428 12-13 26.094201837634294 23.192276452711177 27.40645139631671 23.307070313337817 14-15 23.160538079794406 25.28296186332415 25.68281042814009 25.873689628741353 16-17 22.747914502529827 28.142140196903036 26.499571990458836 22.610373310108297 18-19 22.435532811267375 26.14438934508013 29.166379151179683 22.253698692472813 20-21 23.028312038537642 25.318116459624434 29.414699283666817 22.238872218171103 22-23 22.87224916076091 25.0035434539351 29.528067885117494 22.596139500186496 24-25 23.252782297732665 25.353201449079403 29.314391484560403 22.07962476862752 26-27 22.791953718589028 25.586070817123524 29.49721931077699 22.124756153510457 28-29 22.283870089397695 26.10632796188732 29.38206768189825 22.227734266816736 30-31 22.592096649962794 25.408380781159373 29.32564718959051 22.673875379287324 32-33 22.68963433624952 25.71125114928488 29.12320520399923 22.47590931046637 34-35 23.3433979295155 25.712090383679314 28.540590037728247 22.403921649076935 36-37 23.254159338907094 25.541539305076622 28.732121976191856 22.472179379824432 38-39 22.842841237367193 25.52009220388547 28.892136000731067 22.744930558016275 40-41 22.910201417381572 25.62644535621037 28.970253636702726 22.493099589705334 42-43 23.088236116991613 25.87938620624052 28.273625781277683 22.758751895490178 44-45 23.16268278991352 25.675816808186454 28.20834646579747 22.95315393610256 46-47 23.405035033373554 26.102987114954594 27.775301518268268 22.71667633340358 48-49 23.55124831453759 26.065128318938907 27.4176944179723 22.965928948551202 50-51 22.987842291072596 26.514025471696357 27.559525030632052 22.938607206598995 52-53 23.74996036948693 26.556453432748416 26.917697215420276 22.775888982344373 54-55 23.336426070374532 26.27775886908397 26.978333745180226 23.407481315361267 56-57 23.454643110911352 26.568016217738432 27.003299123652795 22.97404154769742 58-59 23.84031793928792 26.090864840368294 26.97952081581043 23.089296404533357 60-61 23.3618610861931 26.225701833074417 27.802716508486526 22.60972057224596 62-63 22.85086058824736 26.35848736392747 27.82798678858567 22.962665259239504 64-65 22.968353403468463 26.52465577402588 27.351861142142063 23.155129680363594 66-67 23.005186468557618 26.588251091470955 27.122283911130673 23.28427852884076 68-69 22.844053464825823 26.19381092608583 27.435038595457318 23.527097013631032 70-71 23.13228385518171 26.37872223765999 27.217676887298282 23.271317019860017 72-73 23.025794335354334 26.445208251352565 27.18196280140171 23.34703461189139 74-75 22.869789986255206 27.04013218874195 26.696419080087207 23.393658744915637 76-77 22.974321292495567 26.62079473632188 26.87303129598305 23.531852675199506 78-79 23.102910651376437 26.62862759066995 26.801230131125713 23.4672316268279 80-81 23.294722334638188 26.920494663401733 26.660611745924584 23.124171256035496 82-83 22.958080861769602 26.69214228299568 26.769352423260766 23.580424431973952 84-85 22.910840587278127 26.72852140749064 26.693180281703278 23.667457723527956 86-87 23.13806524767672 26.524562525759833 26.515610692219177 23.82176153434427 88-89 22.955764887551915 26.47010553838751 26.353824950625043 24.220304623435528 90-91 23.225788401188137 26.43273817573081 26.434789907345667 23.906683515735384 92-93 23.141846841890295 26.118957610254554 26.63146155008197 24.10773399777318 94-95 23.005466213355763 26.08610917879982 26.704811424031572 24.20361318381285 96-97 22.977957974871458 26.162852501757726 26.771856927320435 24.087332596050377 98-99 22.766831811963755 26.396391607896014 26.237027794951274 24.599748785188957 100-101 22.734916307669923 26.071604111542516 26.580650456397635 24.612829124389922 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 749.0 1 502.5 2 287.5 3 321.0 4 269.5 5 259.0 6 444.0 7 851.0 8 1177.0 9 1247.5 10 1079.5 11 901.5 12 897.5 13 926.0 14 1009.0 15 1068.5 16 1082.5 17 1092.0 18 1129.5 19 1175.5 20 1205.0 21 1333.0 22 1507.5 23 1675.0 24 1918.0 25 2276.5 26 2686.5 27 3162.5 28 3585.5 29 4053.5 30 4747.5 31 5574.5 32 6300.5 33 6507.5 34 6833.0 35 7248.0 36 7723.5 37 8462.0 38 9666.0 39 10541.5 40 11207.0 41 13133.0 42 14808.0 43 15941.5 44 17297.0 45 18553.5 46 18802.5 47 19714.5 48 22647.5 49 23035.5 50 21389.0 51 20486.5 52 20391.0 53 21100.0 54 21031.0 55 19867.5 56 18071.5 57 16435.5 58 15676.5 59 14585.0 60 12139.5 61 9802.0 62 8504.5 63 7003.5 64 5374.0 65 4142.5 66 3000.5 67 2483.0 68 1819.0 69 1096.0 70 861.5 71 691.5 72 577.5 73 431.0 74 324.5 75 284.0 76 173.0 77 89.0 78 50.0 79 27.0 80 17.5 81 10.0 82 8.5 83 6.0 84 3.5 85 3.0 86 2.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01417373643937091 2 0.0 3 0.0 4 0.0 5 0.03897777520827 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004569165036376149 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.59489596290957E-4 24-25 9.324826604849283E-5 26-27 9.324826604849282E-4 28-29 6.527378623394498E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.59489596290957E-4 42-43 0.011562784990013111 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 9.324826604849283E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.459861283879427E-4 84-85 9.324826604849283E-5 86-87 0.0 88-89 0.0 90-91 0.013148005512837489 92-93 0.002237958385163828 94-95 0.0 96-97 0.0 98-99 0.0015852205228243782 100-101 0.019115894539941027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 536203.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.14042175954441 #Duplication Level Percentage of deduplicated Percentage of total 1 66.73035051715506 30.78966517023767 2 16.230143682976692 14.977313495011 3 6.766443561342227 9.36619679197452 4 3.4374514438067725 6.344218375807974 5 1.9629272724097135 4.528514611614815 6 1.1977544103747109 3.3158936195426163 7 0.7946884226784362 2.5667081292867104 8 0.5493475495481374 2.027770210297862 9 0.38323836232422664 1.591450170486922 >10 1.7767609227452705 14.339156802028905 >50 0.11379424930757513 3.6017409412901213 >100 0.053049987931867 4.211622774564122 >500 0.0024297704396274962 0.7511412401840183 >1k 0.001619846959751664 1.588607667672767 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2875 0.5361775297788337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2676 0.49906471989153367 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1803 0.33625324737086515 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1148 0.21409801884733956 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 976 0.182020615326658 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 689 0.1284961106148231 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 640 0.11935778054207082 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 587 0.10947346434093058 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 575 0.10723550595576675 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 553 0.10313258224963306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 1.8649653209698567E-4 0.0 0.0 12-13 0.0 0.0 1.8649653209698567E-4 0.0 0.0 14-15 0.0 0.0 1.8649653209698567E-4 0.0 0.0 16-17 0.0 0.0 1.8649653209698567E-4 0.0 0.0 18-19 0.0 0.0 1.8649653209698567E-4 0.0 0.0 20-21 0.0 0.0 1.8649653209698567E-4 1.8649653209698567E-4 0.0 22-23 0.0 0.0 1.8649653209698567E-4 3.7299306419397133E-4 0.0 24-25 0.0 0.0 1.8649653209698567E-4 3.7299306419397133E-4 0.0 26-27 0.0 0.0 1.8649653209698567E-4 4.662413302424642E-4 0.0 28-29 0.0 0.0 1.8649653209698567E-4 0.0029839445135517706 0.0 30-31 0.0 0.0 1.8649653209698567E-4 0.007832854348073397 0.0 32-33 0.0 0.0 1.8649653209698567E-4 0.013148005512837489 0.0 34-35 0.0 0.0 1.8649653209698567E-4 0.020514618530668424 0.0 36-37 0.0 0.0 1.8649653209698567E-4 0.027881231548499356 0.0 38-39 0.0 0.0 1.8649653209698567E-4 0.034781603236087824 0.0 40-41 0.0 0.0 1.8649653209698567E-4 0.04046974746504589 0.0 42-43 0.0 0.0 1.8649653209698567E-4 0.04615789169400395 0.0 44-45 0.0 0.0 1.8649653209698567E-4 0.05277851858344694 0.0 46-47 0.0 0.0 1.8649653209698567E-4 0.06387506224321758 0.0 48-49 0.0 0.0 1.8649653209698567E-4 0.0767433229579096 0.0 50-51 0.0 0.0 1.8649653209698567E-4 0.08737362528743778 0.0 52-53 0.0 0.0 1.8649653209698567E-4 0.11012620220327002 0.0 54-55 0.0 0.0 1.8649653209698567E-4 0.15964103147501973 0.0 56-57 0.0 0.0 1.8649653209698567E-4 0.23834256801994766 0.0 58-59 0.0 0.0 1.8649653209698567E-4 0.31695085629882713 0.0 60-61 0.0 0.0 1.8649653209698567E-4 0.38455584918398444 0.0 62-63 0.0 0.0 1.8649653209698567E-4 0.4620451582702819 0.0 64-65 0.0 0.0 1.8649653209698567E-4 0.5664832162445939 0.0 66-67 0.0 0.0 1.8649653209698567E-4 0.72882844743502 0.0 68-69 0.0 0.0 1.8649653209698567E-4 0.9195211515041878 0.0 70-71 0.0 0.0 1.8649653209698567E-4 1.0874612786575233 0.0 72-73 0.0 0.0 1.8649653209698567E-4 1.2816041685704853 0.0 74-75 0.0 0.0 1.8649653209698567E-4 1.4992456215276677 0.0 76-77 0.0 0.0 1.8649653209698567E-4 1.735070486364306 0.0 78-79 0.0 0.0 1.8649653209698567E-4 1.9681911514855381 0.0 80-81 0.0 0.0 1.8649653209698567E-4 2.2263023519077665 0.0 82-83 0.0 0.0 1.8649653209698567E-4 2.470892553752963 0.0 84-85 0.0 0.0 1.8649653209698567E-4 2.8456573350018557 0.0 86-87 0.0 0.0 1.8649653209698567E-4 3.289239336594536 0.0 88-89 0.0 0.0 1.8649653209698567E-4 3.8096579094111744 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 80 4.4860138E-4 29.692543 5 ATATAAG 90 8.927569E-4 26.395832 1 GCCTACA 145 7.8563244E-7 26.213793 1 GTATCAA 3945 0.0 23.846577 1 GTTCTAA 140 1.773568E-5 23.75625 1 GTAAGGT 140 1.7748493E-5 23.754034 3 GGTATCA 3005 0.0 23.558609 1 GTACATG 1580 0.0 21.651264 1 GAGTACT 2645 0.0 21.280363 12-13 TATCAAC 4550 0.0 20.3606 2 AACCTAT 140 4.84764E-4 20.356804 7 AACGCAG 4560 0.0 20.310268 6 AGTACTT 2710 0.0 20.15649 12-13 TCAACGC 4600 0.0 20.13929 4 CAACGCA 4630 0.0 20.008797 5 ATCAACG 4665 0.0 19.858679 3 GTACTTT 2805 0.0 19.810656 14-15 CTAGCCT 120 0.004755569 19.795029 4 TCGAACG 60 0.004766325 19.789492 52-53 AGAGTAC 3525 0.0 19.538681 10-11 >>END_MODULE