FastQCFastQC Report
Thu 26 May 2016
SRR937746_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937746_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525662
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.5176330037172174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22310.4244172110595782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14470.2752719428073553No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9150.17406622506477548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8520.1620813374373647No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7590.14438936046356785No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6820.12974116447451023No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5510.10482020766195768No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5440.10348855348113427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.027.6210541
GTATCAA32350.026.6154441
GAATAAC908.8649197E-426.4275531
GTCTTAT1109.373216E-525.947051
GTACATG16150.024.742081
GAGTACT23400.023.75078612-13
GGGTATG1252.2313965E-422.8334061
TATCAAC37650.022.7359242
TAAGGTA1050.002180877822.6435414
TACATGG17550.022.490862
TCAACGC38450.022.2607524
GTACTTT25200.022.2396214-15
CAACGCA38750.022.2068825
ATCAACG38750.022.0863023
ACATGGG17700.022.0273763
AACGCAG39400.021.9444436
AGTACTT23650.021.79247512-13
GATGTGC3800.021.2547558
TTAATGC1908.167241E-620.0197473
AGAGTAC32850.019.81039610-11
Produced by FastQC (version 0.11.5)