FastQCFastQC Report
Thu 26 May 2016
SRR937746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937746_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525662
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.5748941334926245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26270.4997507904318745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17350.33006000053266166No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11420.21724986778576347No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9790.18624134900373243No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.11737580422400706No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.11033706069679756No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.11014682438525136No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG5530.10520068028505009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGG350.00839342427.14141774-75
GTACATG17350.023.550141
GTGTAAG1252.2500988E-422.8052961
GGTATCA32400.022.7290461
GGACCGC1050.002195324322.6178476
TACATGG18400.022.202022
GTATCAA41450.021.8929041
GAGTACT25700.021.16132712-13
ACATGGG19100.021.1396333
ATACGTC1805.1580682E-621.1125
TATATTG1150.003719722620.6530425
AGTACTT26200.020.30426612-13
AGAGTAC35200.020.10546710-11
AATAGGG1200.004758964319.7925012
CGGTGCG600.004764695719.79061756-57
CTTAGAC1456.1382155E-419.6563
GTACTTT27400.019.58837714-15
GTAGTGT1707.946622E-519.5577856
ACGTCTC1951.0313732E-519.4861457
CATGGGG12450.019.458654
Produced by FastQC (version 0.11.5)