FastQCFastQC Report
Thu 26 May 2016
SRR937745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508062
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.5320216823930938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22660.4460085580106365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14390.2832331487101968No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9260.1822612200873122No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8070.1588388818687483No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7650.15057217426219635No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6990.1375816337376147No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.10786085162834456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAG250.004993159357.076271
ATATGCG405.3864997E-447.4978759
CGTTCGA300.00394873631.66993942-43
TAGACTA700.00836082927.1603985
TATGCGC350.00839495927.14030610-11
CGTGTTA350.00841112927.12960290-91
GTGTACA3100.024.5489371
GTATCAA34000.024.4812451
GGTATCA26150.024.3729151
GAGTACT21000.023.2965812-13
TTAGGGC1252.2391531E-422.8214913
AACAGCG1050.00219385222.6202687
TTTAGAC1503.0069381E-522.1875633
GTACTTT22800.021.14280314-15
TATCAAC39150.020.8901882
AGTACTT21900.020.82098812-13
GGAGCGC1150.003719285620.653296
AGAGTAC30950.020.48676710-11
TATGGAC1404.8158877E-420.3763313
ATCAACG40400.020.2418353
Produced by FastQC (version 0.11.5)