##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937745_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 508062 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68182229727868 33.0 31.0 34.0 30.0 34.0 2 31.81969129751881 33.0 31.0 34.0 30.0 34.0 3 31.826814443906454 33.0 31.0 34.0 30.0 34.0 4 35.45554479571391 37.0 35.0 37.0 33.0 37.0 5 35.42621176155666 37.0 35.0 37.0 33.0 37.0 6 35.48684215705957 37.0 35.0 37.0 33.0 37.0 7 35.42464896016628 37.0 35.0 37.0 33.0 37.0 8 35.41272521857569 37.0 35.0 37.0 33.0 37.0 9 37.03025221331255 39.0 37.0 39.0 33.0 39.0 10-11 36.90417409686219 39.0 37.0 39.0 33.0 39.0 12-13 36.781324917037686 39.0 37.0 39.0 33.0 39.0 14-15 37.92672744665021 40.0 38.0 41.0 33.0 41.0 16-17 37.84609260287131 40.0 38.0 41.0 33.0 41.0 18-19 37.77240474587747 40.0 37.5 41.0 33.0 41.0 20-21 37.66531250123016 40.0 37.0 41.0 32.0 41.0 22-23 37.477200617247505 40.0 37.0 41.0 32.0 41.0 24-25 37.27475485275419 39.5 37.0 41.0 31.5 41.0 26-27 37.20619629100385 39.0 36.5 41.0 31.5 41.0 28-29 37.23312410690034 39.0 37.0 41.0 31.5 41.0 30-31 37.03650341887408 39.0 36.0 41.0 31.0 41.0 32-33 36.77867366581244 39.0 35.5 41.0 30.5 41.0 34-35 36.78790580677162 39.0 36.0 41.0 31.0 41.0 36-37 36.67760430813562 39.0 35.5 41.0 30.0 41.0 38-39 36.535110872295114 39.0 35.0 41.0 30.0 41.0 40-41 36.274654471304686 39.0 35.0 41.0 30.0 41.0 42-43 35.99251961374793 39.0 35.0 40.0 29.5 41.0 44-45 35.74190453133673 39.0 35.0 40.0 28.5 41.0 46-47 35.374358838094565 38.0 35.0 40.0 27.0 41.0 48-49 34.96977534237948 38.0 34.0 40.0 26.0 41.0 50-51 33.963139341261495 37.5 33.0 39.5 23.5 40.5 52-53 34.02075435675174 37.5 33.5 39.5 24.0 40.5 54-55 34.3233306171294 38.0 34.0 40.0 24.0 41.0 56-57 34.07537564313017 38.0 33.5 40.0 23.0 41.0 58-59 33.784155280261075 37.0 33.0 40.0 22.5 41.0 60-61 33.41004837992213 37.0 33.0 40.0 21.5 41.0 62-63 33.02268915998441 36.0 32.5 39.0 20.0 41.0 64-65 32.62780133133358 36.0 32.0 39.0 19.0 41.0 66-67 32.18444697694376 35.0 31.5 39.0 17.5 40.0 68-69 31.70028165853774 35.0 31.0 38.0 13.5 40.0 70-71 31.228839196790943 35.0 31.0 37.0 9.0 39.5 72-73 30.784517440784786 35.0 30.0 37.0 7.0 39.0 74-75 30.355878810066486 34.5 30.0 36.0 4.5 39.0 76-77 29.8911136829757 34.0 29.5 36.0 2.0 38.5 78-79 29.391993496856685 34.0 29.0 35.0 2.0 37.0 80-81 28.895294275108157 34.0 29.0 35.0 2.0 37.0 82-83 28.389273356401382 34.0 27.5 35.0 2.0 36.0 84-85 27.967746062488438 33.5 27.0 35.0 2.0 36.0 86-87 27.520929532222446 33.0 26.0 35.0 2.0 35.5 88-89 27.06023871102346 33.0 25.5 35.0 2.0 35.0 90-91 26.563166503300778 32.5 25.0 35.0 2.0 35.0 92-93 26.00676689065508 32.0 24.0 34.5 2.0 35.0 94-95 25.35674878262889 32.0 21.5 34.0 2.0 35.0 96-97 24.60494093240589 31.5 16.5 34.0 2.0 35.0 98-99 23.64031358377521 31.0 2.0 34.0 2.0 35.0 100-101 21.288715747290684 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2796680868895187 1101 2 0.4025317684344323 1101 3 0.18204506542696564 1101 4 0.20655612188067352 1101 5 0.32895783211384355 1101 6 0.3960095394548375 1101 7 0.33728703679930305 1101 8 0.48153929178012334 1101 9 0.5515629457135276 1101 10-11 0.5229903220526708 1101 12-13 0.5847468202662398 1101 14-15 0.6595403750673725 1101 16-17 0.7126335416152614 1101 18-19 0.8440465300399751 1101 20-21 0.6455805589814858 1101 22-23 0.7559510742040274 1101 24-25 0.7837563708482449 1101 26-27 0.9499440617553816 1101 28-29 0.7638055621324114 1101 30-31 0.8925915110301261 1101 32-33 0.8798254089682729 1101 34-35 1.0568875535375923 1101 36-37 0.7706279604516055 1101 38-39 1.044028586811443 1101 40-41 1.0483340922395428 1101 42-43 0.6294130933096724 1101 44-45 0.8725700254342783 1101 46-47 0.6498911692323333 1101 48-49 0.9706214022142632 1101 50-51 0.963796126531534 1101 52-53 0.8151430166262656 1101 54-55 0.5655769412426892 1101 56-57 0.5464169286349758 1101 58-59 0.6108768279410626 1101 60-61 0.5628136631283169 1101 62-63 0.5557485950761247 1101 64-65 0.2570749956802274 1101 66-67 0.2498103900209543 1101 68-69 0.42280958477772757 1101 70-71 0.6562166016137496 1101 72-73 0.5914901173336311 1101 74-75 0.8735688172624023 1101 76-77 0.7573170723701672 1101 78-79 0.8432721104300995 1101 80-81 0.8740125766676456 1101 82-83 0.8818791780803679 1101 84-85 1.3173710707508022 1101 86-87 1.338139205793663 1101 88-89 1.2007394391383492 1101 90-91 1.5940981066507725 1101 92-93 1.8324548936449823 1101 94-95 1.903559741808671 1101 96-97 1.9305055581311166 1101 98-99 2.0263545278813773 1101 100-101 2.046453813840042 1102 1 0.2729813834110324 1102 2 0.288927705155384 1102 3 0.38680885626749983 1102 4 0.38010753028216016 1102 5 0.3076046483576391 1102 6 0.2645393189722469 1102 7 0.301306203796031 1102 8 0.14271525071242763 1102 9 0.3716626638002438 1102 10-11 0.33998355768712685 1102 12-13 0.32953763750411014 1102 14-15 0.5085595032330659 1102 16-17 0.5257262002206033 1102 18-19 0.6339240569692919 1102 20-21 0.6153544847210171 1102 22-23 0.5841159973574221 1102 24-25 0.7710780393399759 1102 26-27 0.8769708474003224 1102 28-29 0.7318553901109439 1102 30-31 0.7851596400734095 1102 32-33 0.689786344951429 1102 34-35 0.8605209730758148 1102 36-37 0.9800456503159296 1102 38-39 0.8314597588096149 1102 40-41 0.9375744019558425 1102 42-43 0.7607202234635224 1102 44-45 1.096726032983021 1102 46-47 1.0892487486612055 1102 48-49 1.004151997183314 1102 50-51 0.9562066015903596 1102 52-53 0.7450840711217381 1102 54-55 0.7730744366412168 1102 56-57 0.6265797083154823 1102 58-59 0.9584475586634014 1102 60-61 0.8343318787647931 1102 62-63 0.9840781617994878 1102 64-65 0.6403877485673135 1102 66-67 0.9198145837722649 1102 68-69 0.8072212558323066 1102 70-71 0.6533295144846818 1102 72-73 0.5804077411852084 1102 74-75 0.6084441308229742 1102 76-77 0.7086889726831451 1102 78-79 0.6052029445983003 1102 80-81 0.6447716456548562 1102 82-83 0.3658186638021981 1102 84-85 0.9257678928192519 1102 86-87 0.8362345437401224 1102 88-89 0.7999022421174651 1102 90-91 0.5654893448170171 1102 92-93 0.8035665483905241 1102 94-95 1.0425127018981364 1102 96-97 1.2821943196542271 1102 98-99 1.575704884738549 1102 100-101 2.259110011660848 1103 1 0.4513835461357054 1103 2 0.37911287148836337 1103 3 0.4316770982242062 1103 4 0.42415381620974557 1103 5 0.385347361038761 1103 6 0.342085968747341 1103 7 0.30724087714391857 1103 8 0.2931709084469176 1103 9 0.5930026029453401 1103 10-11 0.34218549493945716 1103 12-13 0.3171479700192563 1103 14-15 0.46940536385496756 1103 16-17 0.6535950341364725 1103 18-19 0.5688764174535663 1103 20-21 0.4429096179872474 1103 22-23 0.6319364942260677 1103 24-25 0.8056378969681433 1103 26-27 0.6956748715914713 1103 28-29 0.6132241354214614 1103 30-31 0.7198838889073969 1103 32-33 0.5847927735524792 1103 34-35 0.516085577688564 1103 36-37 0.7237612686116108 1103 38-39 0.9574773064397064 1103 40-41 0.9641428109430663 1103 42-43 0.9832742444497953 1103 44-45 0.7886735032059704 1103 46-47 0.953277238109628 1103 48-49 1.0140569443782965 1103 50-51 1.1659411248747134 1103 52-53 1.1774423685416195 1103 54-55 1.1786758030488542 1103 56-57 1.1030992900982213 1103 58-59 1.151066167295447 1103 60-61 1.4314320329758061 1103 62-63 1.1784839592287994 1103 64-65 1.255244792315736 1103 66-67 1.1714179635951254 1103 68-69 1.2865422749069673 1103 70-71 1.1937203583469795 1103 72-73 1.1916696699163403 1103 74-75 1.225751960896762 1103 76-77 1.3782607637554989 1103 78-79 1.6042282857289045 1103 80-81 1.450940840401877 1103 82-83 1.1715599135524073 1103 84-85 1.443204294914107 1103 86-87 1.611014821319955 1103 88-89 1.510051513856883 1103 90-91 1.8208179198544379 1103 92-93 1.8942984285960875 1103 94-95 1.9882692596367768 1103 96-97 2.5022032933026175 1103 98-99 2.357856373496979 1103 100-101 2.9368862812624243 1104 1 0.2914609155747776 1104 2 0.27345569178863016 1104 3 0.11936246250531823 1104 4 0.23594712927965134 1104 5 0.16775502429749167 1104 6 0.24601523097447853 1104 7 0.29979130543902954 1104 8 0.14529948908613477 1104 9 0.3093736078269771 1104 10-11 0.3826791856459124 1104 12-13 0.1900778769917153 1104 14-15 0.3947917505179461 1104 16-17 0.526344412417771 1104 18-19 0.5884214114666477 1104 20-21 0.4792364117608443 1104 22-23 0.6120580747731807 1104 24-25 0.7560348758757627 1104 26-27 0.5880374026774078 1104 28-29 0.5299561976854363 1104 30-31 0.6818402304382118 1104 32-33 0.538652955923304 1104 34-35 0.5381985002270184 1104 36-37 0.6314797912237538 1104 38-39 0.9723230280939319 1104 40-41 0.9627317397447612 1104 42-43 0.9001131502248754 1104 44-45 0.8618889402511982 1104 46-47 1.0885024384412105 1104 48-49 0.8972235649917195 1104 50-51 0.7510604027599541 1104 52-53 0.5966797269279169 1104 54-55 0.9856224733997792 1104 56-57 0.8905451775441051 1104 58-59 0.7474979624506446 1104 60-61 1.1078601438720028 1104 62-63 0.9488846223954255 1104 64-65 0.7825654149555064 1104 66-67 0.794551425877529 1104 68-69 0.8632724950414392 1104 70-71 0.9221794170187962 1104 72-73 0.861488220160421 1104 74-75 1.0531002154790627 1104 76-77 1.3696524925940352 1104 78-79 1.589942571443192 1104 80-81 1.2845210190884373 1104 82-83 1.545256146397577 1104 84-85 1.593470482627108 1104 86-87 2.045156849504533 1104 88-89 2.075028947770484 1104 90-91 1.8807552874513824 1104 92-93 2.1001858744835324 1104 94-95 2.250833503903145 1104 96-97 2.302639877994519 1104 98-99 2.009260997242027 1104 100-101 2.5566928576647463 1105 1 0.09008160522994757 1105 2 -0.01728322446752628 1105 3 -0.020867422552154125 1105 4 0.0922470745451136 1105 5 0.04837899802491563 1105 6 0.11024073726894557 1105 7 0.14307537768861778 1105 8 0.07378334239757578 1105 9 0.17716780596600756 1105 10-11 0.2452736027230884 1105 12-13 0.18315395799883305 1105 14-15 0.03744637558636299 1105 16-17 0.2103400336547736 1105 18-19 0.17670822044311763 1105 20-21 0.31248764328793754 1105 22-23 0.25054740153837685 1105 24-25 0.4093955764778414 1105 26-27 0.44973417333969223 1105 28-29 0.3456048019547282 1105 30-31 0.27020366776716287 1105 32-33 0.28747068991344804 1105 34-35 0.463414701650116 1105 36-37 0.02099812729380801 1105 38-39 0.16951514632053488 1105 40-41 0.43906999918646505 1105 42-43 0.38693854705027064 1105 44-45 0.42653042903061333 1105 46-47 0.46464365354794523 1105 48-49 0.48072657539128727 1105 50-51 0.38527496214693 1105 52-53 0.4899419053625067 1105 54-55 0.4709974049816097 1105 56-57 0.24355557710622833 1105 58-59 0.38936441017915513 1105 60-61 0.3988946266306286 1105 62-63 0.3864106213007261 1105 64-65 0.370239197112042 1105 66-67 0.57891924196948 1105 68-69 0.4989265727644856 1105 70-71 0.6147464668272278 1105 72-73 0.7876403821746543 1105 74-75 0.6943317425726576 1105 76-77 0.8643487159109497 1105 78-79 0.7048029983725819 1105 80-81 0.9858182276270284 1105 82-83 1.2708062284993815 1105 84-85 1.245993744805542 1105 86-87 1.2304168385576233 1105 88-89 0.8716573002609067 1105 90-91 1.268336020894182 1105 92-93 1.3323862028907563 1105 94-95 1.3596129237170516 1105 96-97 1.2997717882298794 1105 98-99 1.2320031975704389 1105 100-101 1.7280721680095716 1106 1 0.18205852513652943 1106 2 0.20930467680096498 1106 3 0.14021239610425695 1106 4 0.23842863184083285 1106 5 0.3349339277313703 1106 6 0.25258324463412407 1106 7 0.20182886946862055 1106 8 0.236519371461398 1106 9 0.3184952166298629 1106 10-11 0.06980307105665418 1106 12-13 0.2809619597084705 1106 14-15 0.11372364199964125 1106 16-17 0.1349442985873779 1106 18-19 0.14384732685272184 1106 20-21 0.25115824822451316 1106 22-23 0.11053274751080266 1106 24-25 0.11443366168490599 1106 26-27 0.13321099402629244 1106 28-29 0.2956780720925707 1106 30-31 0.46231262656797867 1106 32-33 0.48241505040252974 1106 34-35 0.49613058142603705 1106 36-37 0.6999108289774938 1106 38-39 0.7850644433851528 1106 40-41 0.6539117814825701 1106 42-43 0.707102336337563 1106 44-45 0.672370822247025 1106 46-47 1.044313479989306 1106 48-49 1.130979967951312 1106 50-51 0.9269435368901 1106 52-53 0.9764975982995736 1106 54-55 0.9331068217207772 1106 56-57 0.8621539804278129 1106 58-59 1.0660561108095905 1106 60-61 1.1644072886263217 1106 62-63 1.3226808659924671 1106 64-65 1.468230374166282 1106 66-67 1.263754613128519 1106 68-69 1.0991044054948595 1106 70-71 1.4619934940440302 1106 72-73 1.1602152169352244 1106 74-75 0.9034492953500504 1106 76-77 0.9744485464737522 1106 78-79 1.2048258952906572 1106 80-81 1.318235684237365 1106 82-83 1.0741102537775795 1106 84-85 0.8263352141770959 1106 86-87 1.2933184886621873 1106 88-89 1.2126916303734028 1106 90-91 1.3719865528659128 1106 92-93 1.668112264162911 1106 94-95 1.7750736860317744 1106 96-97 1.6157645222745423 1106 98-99 1.6817330139371514 1106 100-101 1.906015566828092 1107 1 0.213039862943198 1107 2 -0.03772225307352528 1107 3 0.09016446751784812 1107 4 0.22346592627213369 1107 5 0.2374292097611459 1107 6 -0.015187776544323128 1107 7 0.2506935610781227 1107 8 0.21504886251972266 1107 9 -0.04270659267427135 1107 10-11 0.22984710544541542 1107 12-13 0.16990243839521924 1107 14-15 0.29281180064325696 1107 16-17 0.38172035226739354 1107 18-19 0.30264446911075993 1107 20-21 0.10129154960545605 1107 22-23 0.3466821349235545 1107 24-25 0.38737572800608433 1107 26-27 0.19070657560973103 1107 28-29 0.26436722525436096 1107 30-31 0.3191334635209131 1107 32-33 0.3731266401338331 1107 34-35 0.3488250666430659 1107 36-37 0.31111638270245123 1107 38-39 0.37862628623930306 1107 40-41 0.5463532936294726 1107 42-43 0.5737597667662229 1107 44-45 0.4642072359905498 1107 46-47 0.549705445960015 1107 48-49 0.4826746928112726 1107 50-51 0.21382982631885028 1107 52-53 0.26085265925873813 1107 54-55 0.11810367640729424 1107 56-57 0.41128452591502906 1107 58-59 0.3356057318742032 1107 60-61 0.5790756827191217 1107 62-63 0.6749372539743632 1107 64-65 0.6726907282387131 1107 66-67 0.49312285444895565 1107 68-69 0.507583271983794 1107 70-71 0.44997390323433706 1107 72-73 0.4158992477293424 1107 74-75 0.367285679138206 1107 76-77 0.5553542469799986 1107 78-79 0.5374989624206314 1107 80-81 0.3872528486623743 1107 82-83 0.6620105323624905 1107 84-85 0.7668288786170798 1107 86-87 0.913615496121075 1107 88-89 0.7962820806025626 1107 90-91 0.7048655631406042 1107 92-93 0.48085003488453637 1107 94-95 0.48549515803848564 1107 96-97 0.8875270960396335 1107 98-99 0.9723562353372657 1107 100-101 1.6192116546572244 1108 1 0.21445908742121134 1108 2 0.2063293750827917 1108 3 0.2097951255156758 1108 4 0.26413203395450324 1108 5 0.23656324228304015 1108 6 0.15718786090396009 1108 7 0.24660770658815068 1108 8 0.1777744607932874 1108 9 0.21933791530499747 1108 10-11 0.2534687738760866 1108 12-13 0.25376726499554536 1108 14-15 0.12041898130056694 1108 16-17 0.3139704244313464 1108 18-19 0.36637039727669674 1108 20-21 0.398153448410973 1108 22-23 0.4957196051988788 1108 24-25 0.19132172187558893 1108 26-27 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-1.1445792485336987 2316 100-101 -0.9557034556993109 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2079.0 3 317.0 4 286.0 5 374.0 6 460.0 7 531.0 8 706.0 9 864.0 10 1093.0 11 1452.0 12 1921.0 13 2543.0 14 2850.0 15 3503.0 16 4459.0 17 5526.0 18 4982.0 19 3865.0 20 3937.0 21 4086.0 22 4557.0 23 4951.0 24 5504.0 25 6070.0 26 7152.0 27 8357.0 28 10167.0 29 12190.0 30 14562.0 31 18222.0 32 23076.0 33 29561.0 34 39633.0 35 54865.0 36 75062.0 37 88796.0 38 54392.0 39 5111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.506355011730506 18.236987827131042 14.737189260069671 23.51946790106878 2 16.197539054478828 19.88658800486503 38.50439905858566 25.411473882070478 3 19.219242933347022 22.536887948291724 30.75371051104171 27.490158607319547 4 13.690878011614183 16.079838617351793 35.25073920990403 34.978544161129996 5 14.637843641933395 35.7967913594088 33.78369309479069 15.781671903867107 6 33.49200838025196 34.16077142507684 16.53245374882128 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25.88786567951899 29.365045699150137 22.5943701014605 30-31 22.464200565609854 25.34009092765343 29.30478519352952 22.8909233132072 32-33 22.396565483782823 25.667523914654005 29.134100614673443 22.80180998688973 34-35 23.16028076654475 25.605249971394638 28.612561106968265 22.621908155092346 36-37 23.03186122953754 25.447365903642144 28.610313109287954 22.910459757532365 38-39 22.69983727008235 25.488830810197744 28.78919078850042 23.022141131219488 40-41 22.7376407857276 25.540584650916482 28.950703176696013 22.77107138665991 42-43 22.87554431178771 25.69565882068656 28.278470650313885 23.15032621721184 44-45 22.75949199799198 25.618951153577207 28.306899962818054 23.31465688561276 46-47 23.040568395430903 25.904045793546377 27.944695061453217 23.1106907495695 48-49 23.173800296504385 25.81274850490179 27.57490779857824 23.438543400015586 50-51 22.848453340778 26.337154644219545 27.61623003232709 23.198161982675362 52-53 23.29969206846913 26.346645460402527 27.227619711959207 23.126042759169128 54-55 22.883041051032688 26.09689700747638 27.19290631842661 23.82715562306433 56-57 23.082376419594596 26.370294557867396 27.27051325283623 23.276815769701777 58-59 23.435384647241122 25.941589271152665 27.210123027560655 23.412903054045554 60-61 23.036666768565368 25.93989225691489 27.984506138081944 23.03893483643779 62-63 22.55477152028876 26.063107442417788 28.01895491550664 23.36316612178681 64-65 22.61599780695267 26.191828532097972 27.705169241327898 23.487004419621464 66-67 22.50677974458853 26.29239189389083 27.501799714018045 23.699028647502587 68-69 22.451817393765527 26.0635281610482 27.69537093925387 23.789283505932403 70-71 22.835690788176173 26.08432404288385 27.492091416129565 23.58789375281041 72-73 22.73097969451542 26.01283354748876 27.606426043403697 23.64976071459212 74-75 22.389396152249354 26.698466639120277 27.147558249272468 23.764578959357898 76-77 22.5815805409969 26.36131949115598 27.19561773971157 23.861482228135547 78-79 22.80289908821545 26.383359833345825 27.14719145897364 23.66654961946508 80-81 22.991694668353833 26.442732070454777 27.012792834198123 23.552780426993262 82-83 22.742080082372468 26.2166754936973 27.241991812066107 23.799252611864123 84-85 22.718840882710577 26.295126393843077 26.970167049140397 24.01586567430595 86-87 22.891898955133357 26.056972105660233 27.018998920123664 24.032130019082746 88-89 22.80525269859116 26.02176625195398 26.76065505865645 24.412325990798408 90-91 22.79845789235426 25.826851175171633 27.01864119762865 24.35604973484546 92-93 22.831453271977388 25.568407079471466 27.19339943627746 24.406740212273686 94-95 22.669529344585484 25.447326298930246 27.324898492951956 24.558245863532317 96-97 22.642586676133003 25.563739991322525 27.267778961069695 24.525894371474777 98-99 22.516067126417738 25.637877492413907 26.891591644009477 24.95446373715887 100-101 22.563423946484072 24.966966694539543 27.44535206601139 25.02425729296499 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1115.0 1 698.5 2 290.5 3 292.5 4 259.0 5 274.5 6 415.0 7 731.5 8 1101.0 9 1199.0 10 1027.0 11 873.0 12 832.0 13 850.0 14 900.5 15 967.0 16 984.0 17 1007.5 18 1043.5 19 1055.5 20 1150.0 21 1316.5 22 1473.0 23 1673.5 24 1917.0 25 2226.5 26 2614.0 27 3047.5 28 3429.5 29 3807.5 30 4443.5 31 5337.5 32 6077.0 33 6224.0 34 6527.0 35 7020.5 36 7429.0 37 8115.5 38 9288.5 39 10178.5 40 10733.0 41 12389.0 42 14198.0 43 15223.5 44 16213.5 45 17415.0 46 17812.5 47 18647.0 48 21270.5 49 21721.5 50 20052.0 51 19126.0 52 19107.0 53 19870.0 54 19962.5 55 18825.5 56 17135.0 57 15570.0 58 14633.5 59 13392.5 60 11391.5 61 9475.0 62 7928.5 63 6413.5 64 5017.5 65 3907.0 66 2955.0 67 2408.5 68 1741.5 69 1124.0 70 894.5 71 724.5 72 602.0 73 461.5 74 333.5 75 254.5 76 174.5 77 110.0 78 61.0 79 37.0 80 26.5 81 15.0 82 11.0 83 9.0 84 5.0 85 5.5 86 3.5 87 2.5 88 4.0 89 4.5 90 3.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33263656797792396 2 0.31295393081946693 3 0.30075069578122354 4 0.28342997508178136 5 0.2814617113659356 6 0.22753128555176336 7 0.22202014714739537 8 0.23560116678673074 9 0.23245194484137763 10-11 0.2360932327156922 12-13 0.23304242395613134 14-15 0.2281217646665171 16-17 0.22674398006542507 18-19 0.22703921962280194 20-21 0.2134581999834666 22-23 0.20528990556270693 24-25 0.20656927697800662 26-27 0.21749314060095026 28-29 0.23904562828946074 30-31 0.2313693997976625 32-33 0.23766784368836874 34-35 0.22910589652443994 36-37 0.20991532529494433 38-39 0.213950265912428 40-41 0.20499466600533006 42-43 0.2193629911310037 44-45 0.21562329007089684 46-47 0.21473757139876629 48-49 0.22743287236597107 50-51 0.238455149174707 52-53 0.2545949116446418 54-55 0.22280745263373367 56-57 0.1895437958359413 58-59 0.19298825733867125 60-61 0.2012549649452232 62-63 0.21050580440969804 64-65 0.19781050344249324 66-67 0.2041089473331995 68-69 0.20834071432226775 70-71 0.20036924627309266 72-73 0.20302640228948435 74-75 0.20440418689057638 76-77 0.21828044608728855 78-79 0.22516936909274854 80-81 0.21621376918565058 82-83 0.21582011644248142 84-85 0.20775023520751404 86-87 0.20853754069385233 88-89 0.2134581999834666 90-91 0.21522963732772774 92-93 0.21158834945341318 94-95 0.2120804153823746 96-97 0.1972200243277395 98-99 0.20637245060642204 100-101 0.19643271884140123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 508062.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.000465803529444 #Duplication Level Percentage of deduplicated Percentage of total 1 69.46800706248463 34.0396470450462 2 15.088551032965283 14.786920578312488 3 6.250535795768411 9.1883749654286 4 3.1586322565667233 6.190978074952911 5 1.752734289501776 4.2942398307702625 6 1.0885411940692091 3.2003415333432836 7 0.6899498262481467 2.3665504007056377 8 0.48756049772119747 1.9112553196591469 9 0.3316253063203342 1.4624815033741065 >10 1.5342495426213194 13.232927237141073 >50 0.10215524661358617 3.481097796055712 >100 0.04424046104348116 3.8443033630011105 >500 0.0020109300474309617 0.7380384525780058 >1k 0.001206558028458577 1.2628438996314717 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2703 0.5320216823930938 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2266 0.4460085580106365 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1439 0.2832331487101968 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 926 0.1822612200873122 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 807 0.1588388818687483 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 765 0.15057217426219635 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 699 0.1375816337376147 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 548 0.10786085162834456 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.968263715845704E-4 0.0 22-23 0.0 0.0 0.0 5.904791147537112E-4 0.0 24-25 0.0 0.0 0.0 7.873054863382816E-4 0.0 26-27 0.0 0.0 0.0 0.0013777846010919928 0.0 28-29 0.0 0.0 0.0 0.004034940617483693 0.0 30-31 0.0 0.0 0.0 0.00984131857922852 0.0 32-33 0.0 0.0 0.0 0.016139762469934774 0.0 34-35 0.0 0.0 0.0 0.0246032964480713 0.0 36-37 0.0 0.0 0.0 0.03424778865571525 0.0 38-39 0.0 0.0 0.0 0.04172719077592892 0.0 40-41 0.0 0.0 0.0 0.047533568737673754 0.0 42-43 0.0 0.0 0.0 0.05491455767209514 0.0 44-45 0.0 0.0 0.0 0.0624923729781011 0.0 46-47 0.0 0.0 0.0 0.07764800359011301 0.0 48-49 0.0 0.0 0.0 0.09329570013108637 0.0 50-51 0.0 0.0 0.0 0.10510528242616059 0.0 52-53 0.0 0.0 0.0 0.13443241179226156 0.0 54-55 0.0 0.0 0.0 0.19357873645342497 0.0 56-57 0.0 0.0 0.0 0.28057599269380507 0.0 58-59 0.0 0.0 0.0 0.3622589369014018 0.0 60-61 0.0 0.0 0.0 0.42986879554070173 0.0 62-63 0.0 0.0 0.0 0.5094850628466605 0.0 64-65 0.0 0.0 0.0 0.6181332199613433 0.0 66-67 0.0 0.0 0.0 0.8013785719065784 0.0 68-69 0.0 0.0 0.0 1.0184780597643595 0.0 70-71 0.0 0.0 0.0 1.206053591884455 0.0 72-73 0.0 0.0 0.0 1.397565651436242 0.0 74-75 0.0 0.0 0.0 1.6357255610535721 0.0 76-77 0.0 0.0 0.0 1.8994728989768965 0.0 78-79 0.0 0.0 0.0 2.136550263550512 0.0 80-81 0.0 0.0 0.0 2.384157839003901 0.0 82-83 0.0 0.0 0.0 2.6542036208179316 0.0 84-85 0.0 0.0 0.0 3.05120241230401 0.0 86-87 0.0 0.0 0.0 3.4995728867736613 0.0 88-89 0.0 0.0 0.0 4.049997834909913 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACTAG 25 0.0049931593 57.07627 1 ATATGCG 40 5.3864997E-4 47.497875 9 CGTTCGA 30 0.003948736 31.669939 42-43 TAGACTA 70 0.008360829 27.160398 5 TATGCGC 35 0.008394959 27.140306 10-11 CGTGTTA 35 0.008411129 27.129602 90-91 GTGTACA 310 0.0 24.548937 1 GTATCAA 3400 0.0 24.481245 1 GGTATCA 2615 0.0 24.372915 1 GAGTACT 2100 0.0 23.29658 12-13 TTAGGGC 125 2.2391531E-4 22.821491 3 AACAGCG 105 0.002193852 22.620268 7 TTTAGAC 150 3.0069381E-5 22.187563 3 GTACTTT 2280 0.0 21.142803 14-15 TATCAAC 3915 0.0 20.890188 2 AGTACTT 2190 0.0 20.820988 12-13 GGAGCGC 115 0.0037192856 20.65329 6 AGAGTAC 3095 0.0 20.486767 10-11 TATGGAC 140 4.8158877E-4 20.376331 3 ATCAACG 4040 0.0 20.241835 3 >>END_MODULE