FastQCFastQC Report
Thu 26 May 2016
SRR937745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508062
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28830.5674504292783165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24920.4904913179887494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.33007782514732453No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10740.2113915230818286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9670.19033110132227957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.13266097444800043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.11317516366112798No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.11041959445894399No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5440.10707354614200629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG459.580961E-442.2571141
CCATATA1152.4956535E-937.175342
GCGGTAT700.00835350727.1652891
CATATAG700.00838567127.1438983
CATATAC1606.2251274E-826.7197743
GTACATG16650.024.2692871
GGTATCA28250.024.2323991
GTATCAA37700.024.0848731
TACATGG16850.023.3985212
GGGTAAG1050.002184035322.6377371
GAGTACT23850.021.9064412-13
TCTACAC2205.621405E-821.5917363
AGCATAC1100.002876183521.5896117
AGTACTT24400.021.41264712-13
TATCAAC43550.021.1603972
TTAGGCA1805.157677E-621.111923
TCAACGC43700.020.9790674
CAACGCA43950.020.8597325
ATCAACG44050.020.8123763
GTACAGA3201.8189894E-1120.7984241
Produced by FastQC (version 0.11.5)