##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937745_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 508062 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.162824615893335 33.0 31.0 34.0 31.0 34.0 2 32.37165739614456 34.0 31.0 34.0 31.0 34.0 3 32.43560234774496 34.0 31.0 34.0 31.0 34.0 4 36.06999736252662 37.0 35.0 37.0 35.0 37.0 5 35.969499785459256 37.0 35.0 37.0 35.0 37.0 6 36.0103215749259 37.0 35.0 37.0 35.0 37.0 7 35.95750321811118 37.0 35.0 37.0 35.0 37.0 8 35.921111990268905 37.0 35.0 37.0 35.0 37.0 9 37.60118450110419 39.0 37.0 39.0 35.0 39.0 10-11 37.52270982675343 39.0 37.0 39.0 35.0 39.0 12-13 37.413911097464485 39.0 37.0 39.0 34.0 39.0 14-15 38.62797552267243 40.0 38.0 41.0 34.0 41.0 16-17 38.58048919226394 40.0 38.0 41.0 34.0 41.0 18-19 38.535386429215336 40.0 38.0 41.0 34.0 41.0 20-21 38.42975562037704 40.0 38.0 41.0 34.0 41.0 22-23 38.36126594785676 40.0 38.0 41.0 34.0 41.0 24-25 38.35024465517988 40.0 38.0 41.0 34.0 41.0 26-27 38.14109400033854 40.0 38.0 41.0 33.5 41.0 28-29 38.083951368140106 40.0 37.5 41.0 33.5 41.0 30-31 38.08777570453999 40.0 37.5 41.0 33.5 41.0 32-33 37.93975243178982 40.0 37.0 41.0 33.0 41.0 34-35 37.763991402624086 40.0 37.0 41.0 33.0 41.0 36-37 37.569676535540935 40.0 36.5 41.0 33.0 41.0 38-39 37.39700666454094 40.0 36.0 41.0 33.0 41.0 40-41 37.187187784168074 40.0 36.0 41.0 32.0 41.0 42-43 36.92842310584142 39.0 35.5 41.0 31.0 41.0 44-45 36.59353878069999 39.0 35.0 41.0 31.0 41.0 46-47 36.218462510481004 39.0 35.0 41.0 30.0 41.0 48-49 35.83312764977503 39.0 35.0 40.0 29.0 41.0 50-51 35.538497270018226 39.0 35.0 40.0 28.0 41.0 52-53 35.22504241608307 38.0 35.0 40.0 27.0 41.0 54-55 34.934377103581845 38.0 34.0 40.0 26.5 41.0 56-57 34.6042087383036 38.0 34.0 40.0 26.0 41.0 58-59 34.30917191208947 37.0 34.0 40.0 26.0 41.0 60-61 33.91082387582618 37.0 33.0 40.0 25.0 41.0 62-63 33.48215768941586 36.0 33.0 39.0 23.5 41.0 64-65 33.054689191476626 36.0 32.5 39.0 22.5 40.0 66-67 32.609218953592276 35.0 32.0 38.5 22.0 40.0 68-69 32.13591254610658 35.0 31.5 38.0 20.0 40.0 70-71 31.70093807448697 35.0 31.0 37.0 19.5 39.5 72-73 31.232880042199575 35.0 31.0 36.5 18.0 39.0 74-75 30.77242836504206 34.0 30.5 36.0 15.5 39.0 76-77 29.516143698997368 33.5 28.5 35.0 13.0 37.0 78-79 30.148502544964984 34.0 30.0 35.0 11.0 37.0 80-81 30.012891143206932 34.0 30.0 35.0 7.5 37.0 82-83 29.673746511252563 34.0 30.0 35.0 6.0 36.0 84-85 29.314551176824878 34.0 29.5 35.0 2.0 36.0 86-87 28.995213182643063 34.0 29.0 35.0 2.0 35.5 88-89 28.62004932468872 34.0 29.0 35.0 2.0 35.0 90-91 28.154614003802685 33.0 28.5 35.0 2.0 35.0 92-93 27.7239146009739 33.0 27.0 35.0 2.0 35.0 94-95 27.244459337639896 33.0 26.5 35.0 2.0 35.0 96-97 26.73372245906996 33.0 25.0 35.0 2.0 35.0 98-99 25.934740248237418 32.5 24.5 34.0 2.0 35.0 100-101 23.616287972727736 30.5 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25894216489091093 1101 2 0.17614948414819054 1101 3 0.09413786733374963 1101 4 0.1328083706983918 1101 5 0.06594541560068024 1101 6 0.12570955859555255 1101 7 0.1290758323789376 1101 8 0.2283967411375869 1101 9 0.1648387402407252 1101 10-11 0.2290928987890979 1101 12-13 0.26891539785747653 1101 14-15 0.3351634776905712 1101 16-17 0.4629085613433901 1101 18-19 0.41399875360171734 1101 20-21 0.38568779685271437 1101 22-23 0.3766777324532171 1101 24-25 0.41012036105173166 1101 26-27 0.17921811505397045 1101 28-29 0.39269627424232567 1101 30-31 0.5544993987547713 1101 32-33 0.5314549224607319 1101 34-35 0.5504973618260891 1101 36-37 0.5429088110531453 1101 38-39 0.5093543184873752 1101 40-41 0.4974848777761949 1101 42-43 0.828559659905622 1101 44-45 0.5545342827604571 1101 46-47 0.6844042157121351 1101 48-49 0.6849549211391235 1101 50-51 0.8730452422468886 1101 52-53 0.7575754110555764 1101 54-55 0.6002295649268632 1101 56-57 0.8425540743565705 1101 58-59 0.7432433098434217 1101 60-61 0.8121251820849764 1101 62-63 0.8236860335217173 1101 64-65 0.9788491114014803 1101 66-67 1.0725821669268143 1101 68-69 0.9278535634446072 1101 70-71 0.8624847079060487 1101 72-73 0.7916828406687877 1101 74-75 1.17429269962458 1101 76-77 0.885037920500487 1101 78-79 0.47418314138525375 1101 80-81 0.5469377777899069 1101 82-83 0.47432861318115727 1101 84-85 0.721954154594215 1101 86-87 0.8701767368263162 1101 88-89 0.9869800065591825 1101 90-91 0.7945561625683659 1101 92-93 1.0447023297239362 1101 94-95 1.1902814081237345 1101 96-97 0.994890883470827 1101 98-99 1.2305480335570955 1101 100-101 2.0477596478157203 1102 1 0.24061003531952707 1102 2 0.25250682282467807 1102 3 0.24266267647923456 1102 4 0.19290715684037707 1102 5 0.14310655661169136 1102 6 0.13068769672528902 1102 7 0.21525742181068352 1102 8 0.20570000372077857 1102 9 0.2149696008140296 1102 10-11 0.34081948173606236 1102 12-13 0.3477889873202855 1102 14-15 0.23614046642180142 1102 16-17 0.24032326898456802 1102 18-19 0.3870791416013759 1102 20-21 0.26710947854698475 1102 22-23 0.1864736365561015 1102 24-25 0.3043912307891503 1102 26-27 0.34132019406735026 1102 28-29 0.468791503461901 1102 30-31 0.2701533521420032 1102 32-33 0.35532707517540985 1102 34-35 0.17488674386906666 1102 36-37 0.39167165441653395 1102 38-39 0.39732679505284807 1102 40-41 0.6230353076357034 1102 42-43 0.4867922267144067 1102 44-45 0.6577821101642911 1102 46-47 0.4239373113995697 1102 48-49 0.30954039988690596 1102 50-51 0.17433715063672395 1102 52-53 0.2712206352271167 1102 54-55 0.17721984940300217 1102 56-57 0.030606931155503503 1102 58-59 0.08107910118098971 1102 60-61 0.15249858127574356 1102 62-63 0.15349350927174754 1102 64-65 0.2252591826175916 1102 66-67 0.24052637450196812 1102 68-69 0.19042705117011138 1102 70-71 -0.18121961709414336 1102 72-73 0.05981304888077332 1102 74-75 0.09439979560712075 1102 76-77 -0.07909773958820665 1102 78-79 -0.08599913147263294 1102 80-81 0.11524738536351009 1102 82-83 0.1510874647145961 1102 84-85 0.23043291013734546 1102 86-87 0.3198225978267608 1102 88-89 0.588583339814857 1102 90-91 0.11512573612599297 1102 92-93 0.5553187335045244 1102 94-95 0.756195141106037 1102 96-97 0.6379832314279277 1102 98-99 -0.04453681224488193 1102 100-101 0.3342627581812785 1103 1 0.3827217082262777 1103 2 0.3425939577046435 1103 3 0.32060145206639135 1103 4 0.295365107002695 1103 5 0.26213936321448017 1103 6 0.2302122441781691 1103 7 0.22757545092602527 1103 8 0.3594585673337676 1103 9 0.2880595899174523 1103 10-11 0.3612778918921222 1103 12-13 0.4134436677566029 1103 14-15 0.47627793668245744 1103 16-17 0.3753766772742537 1103 18-19 0.43709592006160847 1103 20-21 0.3798041746683367 1103 22-23 0.5768499642224612 1103 24-25 0.47156125126264925 1103 26-27 0.4789148792544182 1103 28-29 0.3360851943838128 1103 30-31 0.4058194570130311 1103 32-33 0.4277440406494861 1103 34-35 0.4192640744726077 1103 36-37 0.5584231559944683 1103 38-39 0.4298656965727119 1103 40-41 0.34302919952208555 1103 42-43 0.4187829061854913 1103 44-45 0.6413976609690977 1103 46-47 0.8727648929259146 1103 48-49 0.5415902752738404 1103 50-51 0.5363899292628531 1103 52-53 0.615409066761579 1103 54-55 0.47607627479918335 1103 56-57 0.7161371116464892 1103 58-59 0.8893917913525868 1103 60-61 0.7990556035326506 1103 62-63 0.7095429925328745 1103 64-65 0.9057218910758209 1103 66-67 1.0083709267810121 1103 68-69 1.1162662569645292 1103 70-71 1.0196317483708839 1103 72-73 0.8427223961798198 1103 74-75 1.0334845588800121 1103 76-77 0.8574268945848047 1103 78-79 0.9228199288581855 1103 80-81 0.83322974724285 1103 82-83 1.2061132733798203 1103 84-85 0.9307187095272873 1103 86-87 0.9773553723808135 1103 88-89 1.3337072176374996 1103 90-91 1.390790182003201 1103 92-93 1.533042971027193 1103 94-95 1.3638713809211325 1103 96-97 1.3142324726267773 1103 98-99 1.7942410371712647 1103 100-101 2.4159927116917466 1104 1 0.29473423470688687 1104 2 0.18912614891768698 1104 3 0.24815418302337378 1104 4 0.23845435817705152 1104 5 0.19762061954680377 1104 6 0.19994974072417904 1104 7 0.14498173258763103 1104 8 0.30756577927076734 1104 9 0.17185069243195983 1104 10-11 0.33554518488281104 1104 12-13 0.3417516718092841 1104 14-15 0.44176564046803435 1104 16-17 0.44692867137944603 1104 18-19 0.28246204330007174 1104 20-21 0.39242276175500734 1104 22-23 0.21309017790947848 1104 24-25 -0.048407432535825023 1104 26-27 0.335433254496202 1104 28-29 0.08150105511882089 1104 30-31 0.10333074005622933 1104 32-33 0.11593414841406258 1104 34-35 0.05779958151875064 1104 36-37 0.3347794238400965 1104 38-39 0.07345600697153287 1104 40-41 0.4567696434753046 1104 42-43 0.31539707216136037 1104 44-45 0.12747300406571327 1104 46-47 0.019426033204076987 1104 48-49 0.4017486984109908 1104 50-51 0.37251593092076973 1104 52-53 0.55186112534129 1104 54-55 0.6048890223353283 1104 56-57 0.6577637119964947 1104 58-59 0.24239906776411857 1104 60-61 0.26166037464806635 1104 62-63 0.3047939826774808 1104 64-65 0.2755404413198974 1104 66-67 0.5984823756158733 1104 68-69 0.44276344771704146 1104 70-71 0.48072413116538826 1104 72-73 0.48894142537756835 1104 74-75 0.6334864010095167 1104 76-77 0.8552826558448245 1104 78-79 0.9257691781904143 1104 80-81 0.7392295630299017 1104 82-83 0.38222085374272297 1104 84-85 0.5926105765255407 1104 86-87 0.6490289470331092 1104 88-89 0.9213207388680438 1104 90-91 0.8942791752794292 1104 92-93 0.9504621475953066 1104 94-95 1.026682470540731 1104 96-97 1.12343575161729 1104 98-99 1.1267859390778732 1104 100-101 0.9052402017902992 1105 1 0.12674299223288443 1105 2 0.08688203288046736 1105 3 -0.042945981180238846 1105 4 0.10992671723561642 1105 5 0.04533818933333578 1105 6 -0.11304423847669653 1105 7 -0.023097085146360996 1105 8 0.10006714763420632 1105 9 0.10342157365801796 1105 10-11 0.11821075685872984 1105 12-13 0.14879053333090297 1105 14-15 -0.11162790085654706 1105 16-17 0.12509506437341855 1105 18-19 0.17119220203022678 1105 20-21 0.14994876066031537 1105 22-23 0.09184770390838537 1105 24-25 -0.01129741611546109 1105 26-27 -0.019202760829472254 1105 28-29 -0.2165449218926767 1105 30-31 -0.2088914821659884 1105 32-33 -0.09368818327723005 1105 34-35 -0.017121053401886854 1105 36-37 -0.2654668815786252 1105 38-39 -0.08954891913683838 1105 40-41 -0.3871986104929519 1105 42-43 -0.36519406084357087 1105 44-45 -0.15387346555662162 1105 46-47 -0.6345422207641747 1105 48-49 -0.435082171868153 1105 50-51 -0.1395366142352259 1105 52-53 -0.2749916387528515 1105 54-55 -0.1831350608611686 1105 56-57 -0.2899045967063003 1105 58-59 -0.2629922841789636 1105 60-61 -0.17799479776572724 1105 62-63 -0.36983108574069234 1105 64-65 -0.2598016495394333 1105 66-67 -0.4930556648876916 1105 68-69 -0.7645874006682902 1105 70-71 -0.6694564928083366 1105 72-73 -0.6437898280433387 1105 74-75 -0.40245776976224334 1105 76-77 -0.857043561998637 1105 78-79 -0.755522556894423 1105 80-81 -0.29721816548679314 1105 82-83 -0.7310796388681169 1105 84-85 -0.6766888213945492 1105 86-87 -0.798951348533393 1105 88-89 -0.7540596661675316 1105 90-91 -0.7629074694934239 1105 92-93 -0.7031339115179946 1105 94-95 -0.591976533290687 1105 96-97 -0.6057487037740614 1105 98-99 -0.2709097368936675 1105 100-101 0.3609818547733319 1106 1 -0.19324907016884652 1106 2 -0.07285449991555026 1106 3 -0.03335217250688771 1106 4 -0.20384501575578895 1106 5 -0.1750904409730225 1106 6 -0.1930155410060408 1106 7 -0.07411141861707904 1106 8 -0.14411321902177576 1106 9 -0.4307787896397528 1106 10-11 -0.16831745977663815 1106 12-13 -0.4711244427799741 1106 14-15 -0.4895377175285489 1106 16-17 -0.21637430718249817 1106 18-19 -0.11886977229469409 1106 20-21 -0.26869467326681473 1106 22-23 -0.11037406037547015 1106 24-25 -0.22533780627895084 1106 26-27 -0.24408865785439815 1106 28-29 -0.4409690644030917 1106 30-31 -0.3349428323209267 1106 32-33 -0.5173498584157628 1106 34-35 -0.8877496501260964 1106 36-37 -0.6702385993054136 1106 38-39 -0.28546639864409684 1106 40-41 -0.4656625325823782 1106 42-43 -0.7660618540386039 1106 44-45 -0.8498481873590649 1106 46-47 -0.6522491707420386 1106 48-49 -0.8220171904146625 1106 50-51 -0.7753611322550995 1106 52-53 -1.010352875797011 1106 54-55 -1.0023600765073937 1106 56-57 -0.9682028977549777 1106 58-59 -1.048589359479294 1106 60-61 -1.2625760131341437 1106 62-63 -1.377509185601781 1106 64-65 -1.9544178208694376 1106 66-67 -2.0459437294875293 1106 68-69 -1.746449225400017 1106 70-71 -2.0638101128110833 1106 72-73 -2.096763374470658 1106 74-75 -2.215149531451118 1106 76-77 -1.9775118802963263 1106 78-79 -1.5825897669812825 1106 80-81 -1.964018397508898 1106 82-83 -1.8613287573367714 1106 84-85 -1.8955757258838695 1106 86-87 -1.4953611117792605 1106 88-89 -1.6574423041977902 1106 90-91 -2.367794435041194 1106 92-93 -2.205297670276064 1106 94-95 -1.8635034524340348 1106 96-97 -2.2750294352542717 1106 98-99 -1.956702962458838 1106 100-101 -1.4582164478018065 1107 1 0.13673924723821784 1107 2 0.14814870530866386 1107 3 0.038053932396806545 1107 4 0.04649947095900586 1107 5 -0.09408364110482381 1107 6 0.06002492869410503 1107 7 0.004731106021210962 1107 8 0.06395675671437573 1107 9 0.06598603077782883 1107 10-11 0.036760723729933886 1107 12-13 0.11561132093209636 1107 14-15 0.24383330964096217 1107 16-17 0.1837582453142801 1107 18-19 0.17881542675871742 1107 20-21 0.03872601990037339 1107 22-23 0.2046440626212629 1107 24-25 0.04833442210076555 1107 26-27 0.043666337202971306 1107 28-29 0.041125115269196044 1107 30-31 -0.007972518089133018 1107 32-33 0.17668602811330913 1107 34-35 0.3856567243758917 1107 36-37 0.23283706795037062 1107 38-39 0.30410763604421476 1107 40-41 0.18788493169585507 1107 42-43 0.18154995436687926 1107 44-45 0.21490408175994702 1107 46-47 0.21779696052237796 1107 48-49 0.1788664928972068 1107 50-51 0.35405728430423267 1107 52-53 0.2603949599277584 1107 54-55 0.3420068168215451 1107 56-57 0.11940531600652093 1107 58-59 0.09590783969393613 1107 60-61 0.14668543730470418 1107 62-63 0.07176140122384567 1107 64-65 0.30570334858807513 1107 66-67 0.37353250092915147 1107 68-69 0.3241293625040029 1107 70-71 0.5541953091102556 1107 72-73 0.543139420365037 1107 74-75 0.5180979298315691 1107 76-77 0.4065608513335448 1107 78-79 0.872473438842043 1107 80-81 0.3749939740574675 1107 82-83 0.5573461670259299 1107 84-85 0.48015443617466147 1107 86-87 0.5672119044265926 1107 88-89 0.509566352903132 1107 90-91 0.6122365687631373 1107 92-93 0.2927177866930535 1107 94-95 0.4938378590119079 1107 96-97 0.46800968645438346 1107 98-99 0.1362972172733592 1107 100-101 0.5444757907627285 1108 1 0.09254741482354234 1108 2 0.11793262580459896 1108 3 0.18233717248973136 1108 4 0.22810178570382078 1108 5 -0.03588856474848967 1108 6 -0.038671309158296197 1108 7 0.2877100341459098 1108 8 0.058884661485443246 1108 9 0.031594750932875115 1108 10-11 0.04539806204830654 1108 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50-51 0.3210412765428856 2316 52-53 0.11471826819843756 2316 54-55 0.3435833387715803 2316 56-57 0.1607283817353533 2316 58-59 0.4788814026335473 2316 60-61 0.6065144453085836 2316 62-63 0.5655159488833235 2316 64-65 0.4826679374796541 2316 66-67 0.3386974293793088 2316 68-69 0.56597389318555 2316 70-71 0.6578516273251438 2316 72-73 0.4927816711532991 2316 74-75 0.21880130116312557 2316 76-77 0.33307067427800163 2316 78-79 0.37068469278334604 2316 80-81 0.4559423126458739 2316 82-83 0.4106386724831239 2316 84-85 0.5540084259879059 2316 86-87 0.5277079024862594 2316 88-89 0.9344390184379385 2316 90-91 0.7735572090735907 2316 92-93 0.889310073862589 2316 94-95 1.1042247132442675 2316 96-97 0.9469994996971671 2316 98-99 1.0051730358520636 2316 100-101 1.2452094798240942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 11.0 6 29.0 7 80.0 8 168.0 9 335.0 10 593.0 11 895.0 12 1279.0 13 1854.0 14 2345.0 15 3146.0 16 4264.0 17 5540.0 18 5046.0 19 3628.0 20 3240.0 21 3325.0 22 3385.0 23 3487.0 24 3921.0 25 4674.0 26 5576.0 27 6886.0 28 8447.0 29 10481.0 30 12805.0 31 16190.0 32 20673.0 33 27283.0 34 37388.0 35 53706.0 36 79010.0 37 107054.0 38 67174.0 39 4144.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.06060122750123 18.940812545164917 15.016510423127588 22.98207580420627 2 16.83908656817475 20.49395546212864 37.511957202073766 25.155000767622848 3 19.53245863693801 22.770646102247362 30.741523672307707 26.955371588506914 4 14.212635465750243 16.601713964043757 34.8237026189717 34.3619479512343 5 14.773673487465674 36.332910109152465 33.39868702079704 15.494729382584818 6 33.72895434021832 34.73119422432695 15.993126823104268 15.54672461235046 7 28.99311501352197 33.0071920356177 19.693068956151023 18.306623994709305 8 27.027016387763698 33.575626596753935 19.206907818337132 20.19044919714523 9 26.29305084812483 16.362176269825337 20.29771956965882 37.04705331239101 10-11 26.006766890655076 25.62610468801052 26.28409524821774 22.083033173116668 12-13 26.205135876732662 23.16980753256672 27.36283398355228 23.262222607148342 14-15 23.223740409635045 25.250461557841362 25.70621302124544 25.81958501127815 16-17 22.819459042400336 28.138691734473355 26.48367718900449 22.55817203412182 18-19 22.45533025496888 26.23646326629427 29.2057859080191 22.102420570717747 20-21 23.135040019840154 25.410162766048533 29.392562499938492 22.06223471417282 22-23 22.870622877315085 25.019117081173537 29.562634643792435 22.54762539771894 24-25 23.2204927745039 25.404478193606295 29.415799646499835 21.95922938538997 26-27 22.89422819531705 25.53301210890138 29.456988993339316 22.11577070244225 28-29 22.33550896364939 26.043298717454118 29.489008999078838 22.132183319817656 30-31 22.705988639181832 25.466970566584397 29.323980144155637 22.50306065007814 32-33 22.650680428766567 25.79163566651314 29.21661135845625 22.341072546264037 34-35 23.397439682558428 25.637225378005045 28.64168152705772 22.323653412378803 36-37 23.244446019064714 25.431987229829605 28.85639266305685 22.46717408804883 38-39 22.799874818427675 25.626990406682648 28.970676807161333 22.60245796772835 40-41 22.946336221107092 25.581745517028942 28.98064505970242 22.491273202161548 42-43 23.15688138956644 25.892349155538756 28.23162196800123 22.719147486893576 44-45 23.149241627990286 25.766835543693485 28.225000098413183 22.858922729903046 46-47 23.46987178730155 26.15487873527247 27.72427380910204 22.650975668323944 48-49 23.627332884569206 26.00440497419606 27.543685613173196 22.824576528061534 50-51 23.038034728045005 26.557683904720292 27.504123512484696 22.90015785475001 52-53 23.724663525317776 26.548925131184774 26.944546138069764 22.781865205427685 54-55 23.354826773110368 26.394219603119307 26.932047663474144 23.318905960296185 56-57 23.442808161208674 26.62056993044156 27.022292554845667 22.9143293535041 58-59 23.766489129279496 26.179777271277914 26.982632040971378 23.07110155847121 60-61 23.32599170967323 26.25713003531065 27.914703323610112 22.50217493140601 62-63 22.954580346493145 26.306336628206793 27.864414185670256 22.87466883962981 64-65 22.880868870334723 26.557093425605537 27.436808893402777 23.125228810656967 66-67 22.953399388263637 26.56536013321209 27.156134487523175 23.3251059910011 68-69 22.78855730206156 26.154583495715087 27.591908074211414 23.464951128011936 70-71 23.204746664777133 26.392054513031876 27.207407757320958 23.195791064870036 72-73 23.122079588711614 26.42403879841437 27.204356948561397 23.249524664312624 74-75 22.896910219618867 26.978498687168102 26.745849915955137 23.378741177257893 76-77 23.0189425700013 26.66898921785136 26.971806590534225 23.34026162161311 78-79 23.05732371246029 26.64468116096067 26.812770882293897 23.485224244285146 80-81 23.253461191744314 26.8650282839496 26.679224189173762 23.202286335132328 82-83 23.000334606148883 26.74270755422588 26.76317757745148 23.493780262173757 84-85 23.0256573269181 26.731803138005933 26.690469559295476 23.55206997578049 86-87 23.001031370187103 26.511724946955294 26.65668756962733 23.830556113230276 88-89 22.940212021367472 26.482102578031814 26.358889269419873 24.218796131180838 90-91 23.25323664251365 26.380039014903222 26.391849782780298 23.97487455980284 92-93 23.09192008660565 26.092313748646788 26.731719318964668 24.084046845782893 94-95 23.048466525738984 26.019855844365452 26.740043537993397 24.19163409190217 96-97 23.09914931642201 26.17141215048557 26.75234518621743 23.977093346874987 98-99 22.83758103135558 26.38913355620749 26.221239835098054 24.552045577338873 100-101 22.670164024783343 26.14485164935212 26.59416046180875 24.590823864055785 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 752.0 1 488.0 2 261.5 3 295.5 4 263.5 5 256.0 6 413.5 7 737.5 8 1053.5 9 1199.0 10 1057.5 11 850.5 12 815.5 13 887.5 14 982.5 15 1030.0 16 1063.5 17 1090.5 18 1078.5 19 1083.5 20 1170.0 21 1332.5 22 1506.5 23 1645.5 24 1802.0 25 2177.0 26 2597.0 27 2981.5 28 3381.0 29 3833.0 30 4520.5 31 5265.0 32 5924.5 33 6268.0 34 6613.5 35 7005.5 36 7516.5 37 8161.0 38 9203.5 39 10042.0 40 10528.0 41 12327.0 42 14085.5 43 15185.5 44 16281.0 45 17235.0 46 17665.5 47 18709.0 48 21300.5 49 21726.0 50 20254.0 51 19489.5 52 19469.5 53 19891.0 54 19829.5 55 18863.5 56 17076.0 57 15532.0 58 14832.5 59 13808.0 60 11599.0 61 9349.5 62 7998.5 63 6602.0 64 5057.0 65 3841.5 66 2838.5 67 2383.0 68 1751.0 69 1053.5 70 813.0 71 639.0 72 528.0 73 386.0 74 292.0 75 251.0 76 154.5 77 80.5 78 46.5 79 31.0 80 17.5 81 10.0 82 8.0 83 5.0 84 2.5 85 1.5 86 0.5 87 1.0 88 1.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03956210068849865 2 0.0 3 0.0 4 0.0 5 0.011219103180320513 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00551113840436797 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.952395573768556E-4 22-23 0.0016730241584688482 24-25 0.0 26-27 0.0011809582295074224 28-29 0.001968263715845704 30-31 0.0 32-33 0.0 34-35 0.0 36-37 5.904791147537112E-4 38-39 0.0 40-41 9.84131857922852E-5 42-43 0.020666769016379892 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.936527431691408E-4 84-85 9.84131857922852E-5 86-87 0.0 88-89 0.0 90-91 0.01003814495081309 92-93 0.0023619164590148447 94-95 0.0 96-97 0.0 98-99 4.92065928961426E-4 100-101 0.011711169109281938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 508062.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.18094925898929 #Duplication Level Percentage of deduplicated Percentage of total 1 67.75319985411423 31.96660284451124 2 15.827012036280818 14.934669036103559 3 6.6008131425974765 9.342978898468834 4 3.342215567382125 6.307556123890405 5 1.8803050570301059 4.4357288743579195 6 1.1378915801411542 3.221208294292256 7 0.7433450588961754 2.4550207853990593 8 0.5129176833214962 1.9359954552663878 9 0.37072486834862234 1.5742036082341653 >10 1.6675618019917713 13.86355991897764 >50 0.10752916055734774 3.4113688276160645 >100 0.05271857671610855 4.291403673522936 >500 0.00209200701254399 0.6555845070964473 >1k 0.0016736056100351924 1.6041191522630929 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2883 0.5674504292783165 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2492 0.4904913179887494 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1677 0.33007782514732453 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1074 0.2113915230818286 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 967 0.19033110132227957 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 674 0.13266097444800043 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 575 0.11317516366112798 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 561 0.11041959445894399 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 544 0.10707354614200629 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.968263715845704E-4 0.0 22-23 0.0 0.0 0.0 4.92065928961426E-4 0.0 24-25 0.0 0.0 0.0 5.904791147537112E-4 0.0 26-27 0.0 0.0 0.0 0.0011809582295074224 0.0 28-29 0.0 0.0 0.0 0.003838114245899123 0.0 30-31 0.0 0.0 0.0 0.009644492207643948 0.0 32-33 0.0 0.0 0.0 0.016041349284142487 0.0 34-35 0.0 0.0 0.0 0.024504883262279013 0.0 36-37 0.0 0.0 0.0 0.03414937546992296 0.0 38-39 0.0 0.0 0.0 0.041924017147513495 0.0 40-41 0.0 0.0 0.0 0.047533568737673754 0.0 42-43 0.0 0.0 0.0 0.05511138404367971 0.0 44-45 0.0 0.0 0.0 0.0624923729781011 0.0 46-47 0.0 0.0 0.0 0.07784482996169759 0.0 48-49 0.0 0.0 0.0 0.09388617924584008 0.0 50-51 0.0 0.0 0.0 0.10559734835512202 0.0 52-53 0.0 0.0 0.0 0.13512130409280756 0.0 54-55 0.0 0.0 0.0 0.19446445512555555 0.0 56-57 0.0 0.0 0.0 0.2832331487101968 0.0 58-59 0.0 0.0 0.0 0.3658018115899241 0.0 60-61 0.0 0.0 0.0 0.4336084966008086 0.0 62-63 0.0 0.0 0.0 0.5129295243493904 0.0 64-65 0.0 0.0 0.0 0.6225618133219961 0.0 66-67 0.0 0.0 0.0 0.8072833630541154 0.0 68-69 0.0 0.0 0.0 1.0261542882561576 0.0 70-71 0.0 0.0 0.0 1.2154028445347222 0.0 72-73 0.0 0.0 0.0 1.4081942755018089 0.0 74-75 0.0 0.0 0.0 1.6487161015781537 0.0 76-77 0.0 0.0 0.0 1.914825355960493 0.0 78-79 0.0 0.0 0.0 2.153870984249954 0.0 80-81 0.0 0.0 0.0 2.403938889348151 0.0 82-83 9.84131857922852E-5 0.0 0.0 2.676346587621196 0.0 84-85 1.968263715845704E-4 0.0 0.0 3.0820057394569953 0.0 86-87 1.968263715845704E-4 0.0 0.0 3.5386429215331985 0.0 88-89 1.968263715845704E-4 0.0 0.0 4.101763170636654 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGGG 45 9.580961E-4 42.257114 1 CCATATA 115 2.4956535E-9 37.17534 2 GCGGTAT 70 0.008353507 27.165289 1 CATATAG 70 0.008385671 27.143898 3 CATATAC 160 6.2251274E-8 26.719774 3 GTACATG 1665 0.0 24.269287 1 GGTATCA 2825 0.0 24.232399 1 GTATCAA 3770 0.0 24.084873 1 TACATGG 1685 0.0 23.398521 2 GGGTAAG 105 0.0021840353 22.637737 1 GAGTACT 2385 0.0 21.90644 12-13 TCTACAC 220 5.621405E-8 21.591736 3 AGCATAC 110 0.0028761835 21.589611 7 AGTACTT 2440 0.0 21.412647 12-13 TATCAAC 4355 0.0 21.160397 2 TTAGGCA 180 5.157677E-6 21.11192 3 TCAACGC 4370 0.0 20.979067 4 CAACGCA 4395 0.0 20.859732 5 ATCAACG 4405 0.0 20.812376 3 GTACAGA 320 1.8189894E-11 20.798424 1 >>END_MODULE