FastQCFastQC Report
Thu 26 May 2016
SRR937744_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937744_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451005
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.543674682098868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.4438975177658784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.29090586578862765No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8160.1809292579904879No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6920.15343510604095298No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6410.14212702741654748No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5980.1325927650469507No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.10376825090630926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCA902.3999235E-531.6855552
TAAGCGT650.00582335129.2482055
GGTATCA19600.027.9051271
GTATCAA26700.027.4316311
CTAGAGT700.0083573527.1620674
GTACATG11500.026.8817631
GTATATG1309.905836E-625.6092531
CCTAGGG1151.2748885E-424.797392
GTGTTAT1351.3273091E-524.660761
GCCTAGG1801.9219442E-723.780021
TCGCGCA1000.001651695823.750979
TACATGG13450.023.3224542
TATCAAC31850.022.9807322
GTGTAGC1452.307614E-522.960021
TCAACGC31400.022.858524
ACATGGG14050.022.6647573
CAACGCA31700.022.639685
GTTCGCG1050.002193640522.6199727
ATCAACG31750.022.6040273
AACGCAG31550.022.5841266
Produced by FastQC (version 0.11.5)