FastQCFastQC Report
Thu 26 May 2016
SRR937744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451005
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25250.5598607554239975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22710.5035420893338212No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.35010698329286816No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10480.23236992937994033No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9250.20509750446225652No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.12549750002771587No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.10775933747962883No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.10398997793816032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCA250.001614270338.0065286-87
TAGTACT600.00394939931.665084
GTGTAAG953.474207E-530.0151331
TAGGGAG1004.9482198E-528.498575
CGCGCTC350.00839268627.14149558-59
GTACATG15200.025.0126111
GGTATCA26700.024.5629461
TACATGG15950.023.5254652
GTATCAA34100.023.2741971
ACATGGG16350.022.0784033
GAGTACT24650.021.77375412-13
AGTACTT24550.020.99181712-13
AGGTAAG1150.00372090320.6511382
ATAGCCT1150.00372090320.6511388
AGTACCC1150.00372090320.6511383
GTACTTT26000.020.55185314-15
AGAGTAC32250.020.39820310-11
TAGAGTG1404.8470945E-420.3561217
CATGGGG10500.019.9037654
GTATGGA1707.909471E-519.568691
Produced by FastQC (version 0.11.5)