##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937744_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 451005 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17563219919956 33.0 31.0 34.0 31.0 34.0 2 32.40347224531879 34.0 31.0 34.0 31.0 34.0 3 32.51025820112859 34.0 31.0 34.0 31.0 34.0 4 36.1511890112083 37.0 35.0 37.0 35.0 37.0 5 35.97341049434042 37.0 35.0 37.0 35.0 37.0 6 36.06868216538619 37.0 35.0 37.0 35.0 37.0 7 36.02648085941397 37.0 35.0 37.0 35.0 37.0 8 36.01901974479219 37.0 35.0 37.0 35.0 37.0 9 37.68568419418853 39.0 37.0 39.0 35.0 39.0 10-11 37.63659050343122 39.0 37.0 39.0 35.0 39.0 12-13 37.554470571279694 39.0 37.0 39.0 35.0 39.0 14-15 38.783591091007864 40.0 38.0 41.0 35.0 41.0 16-17 38.74966242059401 40.0 38.0 41.0 35.0 41.0 18-19 38.71307635170342 40.0 38.0 41.0 34.5 41.0 20-21 38.621969822950966 40.0 38.0 41.0 34.0 41.0 22-23 38.47673307391271 40.0 38.0 41.0 34.0 41.0 24-25 38.389864857374086 40.0 38.0 41.0 34.0 41.0 26-27 38.27595037749027 40.0 38.0 41.0 34.0 41.0 28-29 38.14375782973581 40.0 38.0 41.0 34.0 41.0 30-31 38.216267003691755 40.0 38.0 41.0 34.0 41.0 32-33 38.12495981197548 40.0 37.0 41.0 34.0 41.0 34-35 37.96534739082715 40.0 37.0 41.0 33.0 41.0 36-37 37.77835168124522 40.0 37.0 41.0 33.0 41.0 38-39 37.608578618862325 40.0 37.0 41.0 33.0 41.0 40-41 37.398497799359205 40.0 36.0 41.0 33.0 41.0 42-43 37.159653440649215 40.0 36.0 41.0 32.0 41.0 44-45 36.84379330606091 39.0 36.0 41.0 31.0 41.0 46-47 36.434465249830936 39.0 35.0 41.0 30.5 41.0 48-49 36.06034190308311 39.0 35.0 41.0 30.0 41.0 50-51 35.75310695003381 39.0 35.0 40.0 29.0 41.0 52-53 35.48666977084511 38.5 35.0 40.0 28.0 41.0 54-55 35.21646766665558 38.0 35.0 40.0 28.0 41.0 56-57 34.889185264021464 38.0 34.0 40.0 27.0 41.0 58-59 34.58355783195309 37.5 34.0 40.0 26.0 41.0 60-61 34.17907229409873 37.0 34.0 40.0 26.0 41.0 62-63 33.78621079588918 36.5 33.0 39.5 25.5 41.0 64-65 33.369103446746706 36.0 33.0 39.0 24.5 40.5 66-67 32.92443210163967 35.0 32.5 39.0 23.0 40.0 68-69 32.46021884458044 35.0 32.0 38.0 22.0 40.0 70-71 32.04419241471824 35.0 32.0 37.0 20.5 39.5 72-73 31.623814591855968 35.0 31.0 37.0 20.0 39.0 74-75 31.153519362313055 34.5 31.0 36.0 18.0 39.0 76-77 29.928058447245597 33.5 29.5 35.0 17.5 37.0 78-79 30.515198279398234 34.0 31.0 35.0 17.5 37.0 80-81 30.36322213722686 34.0 31.0 35.0 14.0 37.0 82-83 30.059168967084624 34.0 30.5 35.0 9.5 36.0 84-85 29.70864846287735 34.0 30.0 35.0 7.0 36.0 86-87 29.3149189033381 34.0 29.5 35.0 2.0 35.5 88-89 28.92452744426337 34.0 29.0 35.0 2.0 35.0 90-91 28.467951574816244 33.5 29.0 35.0 2.0 35.0 92-93 28.094156384075568 33.0 28.5 35.0 2.0 35.0 94-95 27.664954933980777 33.0 27.0 35.0 2.0 35.0 96-97 27.139169188811657 33.0 26.5 35.0 2.0 35.0 98-99 26.37386614339087 33.0 25.0 34.5 2.0 35.0 100-101 24.085683085553377 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34370459302478906 1101 2 0.24097030028618605 1101 3 0.2949919756775685 1101 4 0.1712683345892856 1101 5 0.030588529191348357 1101 6 0.09831771677004042 1101 7 0.1266733091931016 1101 8 0.09422734341754335 1101 9 0.3723868465622928 1101 10-11 0.31821369946663225 1101 12-13 0.39145696430023946 1101 14-15 0.3559123432688054 1101 16-17 0.19998988647746074 1101 18-19 0.32590850102794633 1101 20-21 0.4114369654800143 1101 22-23 0.351652108275573 1101 24-25 0.2924199688664757 1101 26-27 0.32532057533911285 1101 28-29 0.3727757283890796 1101 30-31 0.4338850957537588 1101 32-33 0.5745375790887621 1101 34-35 0.3798678611357005 1101 36-37 0.6365591016170455 1101 38-39 0.6514660685852931 1101 40-41 0.7787565258069762 1101 42-43 0.853215533358977 1101 44-45 0.82789275035163 1101 46-47 0.963169763418783 1101 48-49 1.334452542738397 1101 50-51 1.43949152610503 1101 52-53 1.5514649082599163 1101 54-55 1.605034617275912 1101 56-57 1.3186354492222918 1101 58-59 1.4885946618646244 1101 60-61 1.5159666118152515 1101 62-63 1.5541966445537696 1101 64-65 1.4649695947892312 1101 66-67 1.566623382632308 1101 68-69 1.7951833495892942 1101 70-71 1.7281820004362167 1101 72-73 1.6116831668119467 1101 74-75 1.4530065830080332 1101 76-77 1.4636609210329468 1101 78-79 1.3384146034359325 1101 80-81 1.611787624564311 1101 82-83 1.5698363009007075 1101 84-85 1.5694793882337628 1101 86-87 1.832315865628054 1101 88-89 1.8870350657515083 1101 90-91 2.0012288190529866 1101 92-93 2.006473755227013 1101 94-95 1.8775986260860265 1101 96-97 2.1012727450742226 1101 98-99 2.2595771511581155 1101 100-101 2.8749409588937986 1102 1 0.11489970280522499 1102 2 0.20323327433808203 1102 3 -0.04731091853400926 1102 4 -0.06781599674803829 1102 5 -0.30074879615795425 1102 6 -0.07607100578485415 1102 7 -0.052480882423665776 1102 8 0.11633313184069749 1102 9 0.16253155714113632 1102 10-11 0.043388350165237455 1102 12-13 -0.0015258201309009678 1102 14-15 -0.2266101118210173 1102 16-17 -0.03765357659639079 1102 18-19 0.15015999803393498 1102 20-21 0.07223412116863415 1102 22-23 0.12245425398415932 1102 24-25 0.12097535808803173 1102 26-27 -0.053347089331545305 1102 28-29 -0.11556009995423011 1102 30-31 -0.06555352700073058 1102 32-33 0.1334946649171087 1102 34-35 0.07235164357083335 1102 36-37 -0.15283710596778377 1102 38-39 -0.32998353221310595 1102 40-41 -0.26489367379381434 1102 42-43 0.2177053038180503 1102 44-45 0.1609266824873572 1102 46-47 0.2638683662132024 1102 48-49 0.18792060661065335 1102 50-51 0.39103219476770334 1102 52-53 0.2876487904954459 1102 54-55 0.25058102446153896 1102 56-57 0.40476943125822373 1102 58-59 0.401412775637084 1102 60-61 0.21939101301286001 1102 62-63 0.1826459958511748 1102 64-65 0.15048606185509783 1102 66-67 0.2856852588798091 1102 68-69 -0.015507768175176295 1102 70-71 0.16915630183342145 1102 72-73 0.19841595124308853 1102 74-75 0.1681762436866805 1102 76-77 0.09007060167166969 1102 78-79 0.06544179904471648 1102 80-81 0.20166412157029967 1102 82-83 0.4276643947130836 1102 84-85 0.4337757954193968 1102 86-87 0.44171581572785357 1102 88-89 0.4867606146537007 1102 90-91 0.4759481304302291 1102 92-93 0.9597609308757171 1102 94-95 1.0094973286808369 1102 96-97 1.0910556792059616 1102 98-99 0.791962380699033 1102 100-101 1.5156408091931972 1103 1 0.24183706314430964 1103 2 0.26184513428352574 1103 3 0.2087784962618926 1103 4 0.06349111015369857 1103 5 -0.019212267621398382 1103 6 0.0992556317766784 1103 7 -0.1041782844324004 1103 8 -0.18070625552004316 1103 9 0.158857630094829 1103 10-11 0.06796469548256567 1103 12-13 0.1507572631050138 1103 14-15 0.294200855885002 1103 16-17 0.2447815531441293 1103 18-19 0.1165459512271525 1103 20-21 0.2560875298890437 1103 22-23 0.32639895422511245 1103 24-25 0.17274035399263 1103 26-27 0.3794208409433608 1103 28-29 -0.020028056471481648 1103 30-31 0.10939140385469415 1103 32-33 0.09908937191744371 1103 34-35 0.2386286513083462 1103 36-37 0.3250219170220916 1103 38-39 0.11470478040467924 1103 40-41 0.13029205037537395 1103 42-43 0.3066310380070405 1103 44-45 0.4577732284392795 1103 46-47 0.8103809187972715 1103 48-49 0.7781942426055934 1103 50-51 0.22652256860170894 1103 52-53 0.32007795606868683 1103 54-55 0.10329988952027236 1103 56-57 0.4002963057959974 1103 58-59 0.31486873224974943 1103 60-61 0.21769718950449857 1103 62-63 0.19176719568258704 1103 64-65 0.45437032520091947 1103 66-67 0.02943878767878516 1103 68-69 0.10125074666765244 1103 70-71 0.04533422487180516 1103 72-73 0.24766009244276077 1103 74-75 0.22644615804123447 1103 76-77 0.13106629951900217 1103 78-79 0.5763005596112301 1103 80-81 0.44162245192154614 1103 82-83 0.24152869797906362 1103 84-85 0.3877562806640462 1103 86-87 0.5667406664248666 1103 88-89 0.6244354641578802 1103 90-91 0.6344916012575013 1103 92-93 0.8281993528365739 1103 94-95 0.6774741240939903 1103 96-97 0.8156900757515153 1103 98-99 0.8604071644383247 1103 100-101 1.5792943785485107 1104 1 0.19682959302478764 1104 2 0.28849634195285034 1104 3 0.27324718401089854 1104 4 0.1803829179226213 1104 5 0.022776029191348357 1104 6 0.19961980010337044 1104 7 0.16729830919310018 1104 8 -0.04040807324912521 1104 9 0.2518139298956257 1104 10-11 0.06626057446663225 1104 12-13 0.02466008930023378 1104 14-15 0.17362067660213398 1104 16-17 0.2477112406441293 1104 18-19 0.31497100102794917 1104 20-21 0.30707498631334573 1104 22-23 0.26441252494224443 1104 24-25 0.20954236469980714 1104 26-27 0.13560703367244997 1104 28-29 0.2565647908890796 1104 30-31 0.3554345749204302 1104 32-33 0.47043601658876355 1104 34-35 0.3305189028023676 1104 36-37 0.486298684950377 1104 38-39 0.30003377691862454 1104 40-41 0.2697070466403133 1104 42-43 0.28225199169230564 1104 44-45 0.06265837535163143 1104 46-47 0.3719588259187887 1104 48-49 0.42130150107173137 1104 50-51 0.4126686094383629 1104 52-53 0.15205084575991634 1104 54-55 0.009787221442579153 1104 56-57 0.17879169922229465 1104 58-59 0.3713420576979587 1104 60-61 0.04780254931525718 1104 62-63 0.2859023737204325 1104 64-65 0.2589800114558969 1104 66-67 0.45171452846563653 1104 68-69 0.5210948079226299 1104 70-71 0.30344241710288244 1104 72-73 0.2627248334786181 1104 74-75 0.5026810621747018 1104 76-77 0.30662967103294747 1104 78-79 0.32220366593593397 1104 80-81 0.33457408289764246 1104 82-83 0.30395088423404104 1104 84-85 0.1796356382337656 1104 86-87 0.40829242812805333 1104 88-89 0.5233631907515068 1104 90-91 0.24738767321965227 1104 92-93 0.5808878177270138 1104 94-95 0.5809189385860236 1104 96-97 0.750100870074224 1104 98-99 1.0565172553247812 1104 100-101 1.7734435630604644 1105 1 0.11761023504813295 1105 2 0.08358541053261348 1105 3 -0.10910173379065924 1105 4 0.07403260177476056 1105 5 -0.02583170427168824 1105 6 0.07408782053138907 1105 7 -0.06488797485359044 1105 8 0.07649063784037224 1105 9 0.04195558845593439 1105 10-11 0.022737045770135467 1105 12-13 0.019484688035639408 1105 14-15 0.14948400345038237 1105 16-17 0.030773771042966303 1105 18-19 0.1115116138684158 1105 20-21 0.04466086301567884 1105 22-23 0.023121790506451134 1105 24-25 0.14688657068229816 1105 26-27 0.11541460906155265 1105 28-29 0.07401600076261872 1105 30-31 0.11380565347101168 1105 32-33 -0.016367090171939935 1105 34-35 0.046327815740113465 1105 36-37 0.24892939993092256 1105 38-39 0.09031982993418808 1105 40-41 0.09207526770062913 1105 42-43 0.26990246591409317 1105 44-45 0.480421933231014 1105 46-47 0.2464382459090615 1105 48-49 0.0013948254880702393 1105 50-51 0.22256950924380448 1105 52-53 -0.07687896333541744 1105 54-55 0.26973143958460355 1105 56-57 0.2847468305452523 1105 58-59 0.36194501854426164 1105 60-61 0.44691262348840866 1105 62-63 0.45117702717374186 1105 64-65 0.45330454939364273 1105 66-67 0.3561823968994915 1105 68-69 0.26555462066581725 1105 70-71 0.5902402040678645 1105 72-73 0.6300643276420423 1105 74-75 0.49457597341011095 1105 76-77 0.3119342673364507 1105 78-79 0.7898859069378794 1105 80-81 0.6198899267692362 1105 82-83 0.6373500817048594 1105 84-85 0.525559154770729 1105 86-87 0.8929068189354474 1105 88-89 0.8563444042729067 1105 90-91 0.563797723073737 1105 92-93 0.9498954478340167 1105 94-95 0.6898797544906898 1105 96-97 1.0919585427395937 1105 98-99 1.3331504326107755 1105 100-101 1.4337404076616345 1106 1 -0.10676851454493175 1106 2 0.05666585141500491 1106 3 0.06734423331369044 1106 4 0.027634536448516656 1106 5 -0.266224219812635 1106 6 -0.31309496583288166 1106 7 -0.28545318483080706 1106 8 -0.22652298858776732 1106 9 -0.458672250383259 1106 10-11 -0.2846249459516983 1106 12-13 -0.5384857647834309 1106 14-15 -0.2257194628400967 1106 16-17 -0.250238367174596 1106 18-19 -0.01194010454973693 1106 20-21 -0.20905191234203357 1106 22-23 -0.1267882967709042 1106 24-25 -0.14498872967270415 1106 26-27 -0.1763560514869127 1106 28-29 -0.05787666204916775 1106 30-31 -0.10275999056761265 1106 32-33 -0.4905520623455004 1106 34-35 -0.6627657710024124 1106 36-37 -0.26163146943209625 1106 38-39 -0.3404745024638487 1106 40-41 -0.435445001417456 1106 42-43 -0.6814167707578918 1106 44-45 -0.46354151259657783 1106 46-47 -0.4645194796091019 1106 48-49 -0.28355874544221393 1106 50-51 -0.35116667442617455 1106 52-53 -0.0793244343695676 1106 54-55 -0.3618395527108049 1106 56-57 -0.35667580575778146 1106 58-59 -0.34349780161478805 1106 60-61 -0.060191256710638186 1106 62-63 -0.15683838200665434 1106 64-65 -0.5894543759943005 1106 66-67 -0.20064089359611614 1106 68-69 -0.5252432772367328 1106 70-71 -0.32119963967002363 1106 72-73 0.023556506785389786 1106 74-75 -0.06239846347271083 1106 76-77 -0.2972205530706411 1106 78-79 0.14900972295784598 1106 80-81 0.12787743200123103 1106 82-83 -0.1678338518217366 1106 84-85 -0.41403654802121537 1106 86-87 -0.12150634552732598 1106 88-89 -0.010026687236543097 1106 90-91 0.017957736715668915 1106 92-93 0.33019138470908516 1106 94-95 -0.21694818666298232 1106 96-97 0.15234346220569606 1106 98-99 0.5108055708128312 1106 100-101 1.0902505538473157 1107 1 -0.1125454069752152 1107 2 -0.03707457150868976 1107 3 -0.04042469098909862 1107 4 -0.009340639769689574 1107 5 -0.18671916311634362 1107 6 -0.006329719127400324 1107 7 0.0050386938084869826 1107 8 -0.10897778478758369 1107 9 -0.033702897027453105 1107 10-11 -0.1692863005333649 1107 12-13 -0.05253342031515018 1107 14-15 -0.0400812464747915 1107 16-17 -0.07156460070202542 1107 18-19 -0.12697611435666545 1107 20-21 -0.07758547041742503 1107 22-23 -0.09442160967314095 1107 24-25 0.08777253296903353 1107 26-27 -0.2182691682506217 1107 28-29 -0.11808965622630296 1107 30-31 -0.14223669911803682 1107 32-33 -0.29565472860354447 1107 34-35 -0.18351354912071116 1107 36-37 -0.2820306419727032 1107 38-39 0.02854940191862454 1107 40-41 -0.09704475624430131 1107 42-43 0.013231558999997617 1107 44-45 -0.2336457111868313 1107 46-47 -0.2089456211965981 1107 48-49 -0.02740643162056955 1107 50-51 0.0485460132845148 1107 52-53 0.07670529287530314 1107 54-55 0.03660513009642585 1107 56-57 -0.18593185847001337 1107 58-59 -0.20619700480204273 1107 60-61 0.18159161181525718 1107 62-63 -0.12505015031803168 1107 64-65 -0.353459892390255 1107 66-67 -0.3875432840343649 1107 68-69 -0.056579470923523445 1107 70-71 -0.018372486743270855 1107 72-73 -0.06660208959830527 1107 74-75 -0.2360959810945289 1107 76-77 -0.26566600204397517 1107 78-79 -0.18346039656406532 1107 80-81 -0.09021558056389622 1107 82-83 -0.30091690422749906 1107 84-85 -0.24975138099700445 1107 86-87 -0.19003990360271317 1107 88-89 -0.2216187804023413 1107 90-91 -0.3294602835111178 1107 92-93 -0.04857432169606213 1107 94-95 0.15596401070141042 1107 96-97 0.28348428353575983 1107 98-99 0.6329745870555534 1107 100-101 1.484155702483541 1108 1 -0.029872139648482232 1108 2 0.15311964022017577 1108 3 0.06205055653565239 1108 4 -0.07838100534470271 1108 5 -0.20703523318488948 1108 6 -7.128112827672339E-4 1108 7 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0.3522204461710814 2316 62-63 -0.022254020067911995 2316 64-65 -0.16924564038459522 2316 66-67 -0.16446046194847241 2316 68-69 0.1816225111434875 2316 70-71 0.40311170391269613 2316 72-73 0.44504845311051966 2316 74-75 0.5960536177728599 2316 76-77 0.4812606142844835 2316 78-79 -0.03560763582786919 2316 80-81 0.20062325851113982 2316 82-83 0.19459984895796723 2316 84-85 -0.14348073446562282 2316 86-87 -0.2651342877461822 2316 88-89 -0.30844039437119264 2316 90-91 -0.3708123363662388 2316 92-93 -0.62709603004906 2316 94-95 -0.48823726348452823 2316 96-97 -0.5335815494043032 2316 98-99 -1.055811396899145 2316 100-101 -1.4167704419241964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5.0 5 7.0 6 28.0 7 54.0 8 147.0 9 246.0 10 435.0 11 677.0 12 1041.0 13 1511.0 14 1924.0 15 2549.0 16 3581.0 17 4820.0 18 4495.0 19 2934.0 20 2780.0 21 2674.0 22 2563.0 23 2703.0 24 3108.0 25 3771.0 26 4383.0 27 5236.0 28 6774.0 29 8541.0 30 10613.0 31 13486.0 32 17344.0 33 22998.0 34 32578.0 35 48010.0 36 72621.0 37 99137.0 38 63101.0 39 4130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.316666962431896 18.932672002271477 14.912557008748736 22.838104026547885 2 16.766776421547434 20.49555991618718 37.69936031751311 25.03830334475228 3 19.52705624106163 22.825689293910266 30.890123169366195 26.757131295661914 4 14.108047582621037 16.680081152093656 34.700058757663435 34.51181250762186 5 14.8340267366871 36.38194253475148 33.22836182348819 15.555668905073228 6 33.678340594893626 34.594073236438625 16.00824824558486 15.719337923082893 7 29.09612975465904 32.90783915921109 19.703329231383243 18.29270185474662 8 27.161117947694592 33.61270939346571 19.174510260418398 20.051662398421303 9 26.416780301770494 16.335960798660768 20.29356659017084 36.9536923093979 10-11 26.03740535027328 25.55470560193346 26.29671511402313 22.111173933770136 12-13 26.16355129825495 23.3076121421761 27.400718419477148 23.128118140091797 14-15 23.28610547554905 25.383532333344423 25.635192514495404 25.695169676611123 16-17 22.82458065875101 28.177403798184052 26.547266660014856 22.45074888305008 18-19 22.52236671433798 26.13762596866997 29.31497433509606 22.025032981895986 20-21 23.15250674607486 25.367569617053544 29.474083320953522 22.005840315918075 22-23 22.95752240841237 24.993653028530886 29.653050703710065 22.395773859346676 24-25 23.258108575533697 25.401659408785733 29.374573174517298 21.965658841163272 26-27 22.791206622638434 25.55839986164034 29.59566384367132 22.05472967204991 28-29 22.36382302203577 26.049570504263915 29.43542486152809 22.151181612172223 30-31 22.626517724898225 25.490904736986813 29.351291784551798 22.53128575356316 32-33 22.631456414008714 25.775213135109365 29.28304564250951 22.310284808372412 34-35 23.326016341282248 25.607476635514022 28.75799603108613 22.308510992117604 36-37 23.315484307269323 25.465460471613397 28.810988791698538 22.408066429418742 38-39 22.73999179609982 25.663019256992715 29.03038768971519 22.56660125719227 40-41 22.995643063824126 25.60603540980699 28.951452866376204 22.446868659992685 42-43 23.104265665571997 25.926164094081024 28.34593201810308 22.6236382222439 44-45 23.180341681356083 25.733861043669137 28.257779847230076 22.828017427744705 46-47 23.442090442456294 26.294941297768315 27.69581268500349 22.5671555747719 48-49 23.63898404674006 26.197270540237916 27.394485648717865 22.769259764304163 50-51 23.03511047549362 26.61500426824536 27.524417689382602 22.825467566878416 52-53 23.638899390139123 26.699290140120553 26.989863737501512 22.671946732238812 54-55 23.390982361614615 26.393277236394276 26.8872850633585 23.328455338632608 56-57 23.392423587321648 26.738284497954567 27.046595935743508 22.822695978980278 58-59 23.71725368898349 26.25314575226439 26.942162503741645 23.087438055010477 60-61 23.366370661079145 26.356803139654772 27.871863948293257 22.404962250972826 62-63 22.883227458675627 26.444496180751877 27.784392634228 22.887883726344498 64-65 22.950189022294655 26.69294131994102 27.376082305074224 22.9807873526901 66-67 23.04730546224543 26.769437145929647 27.12896752807619 23.05428986374874 68-69 22.92258400682919 26.31789004556491 27.433509606323653 23.326016341282248 70-71 23.167814104056497 26.51356415117349 27.196039955211138 23.122581789558875 72-73 23.047748916309132 26.545825434307822 27.125198168534716 23.281227480848326 74-75 22.968259775390518 27.113003181782908 26.626312346869767 23.292424695956807 76-77 22.94131994102061 26.794603164044744 26.92741765612355 23.3366592388111 78-79 22.952849746676865 26.79693129787918 26.840722386669768 23.409496568774184 80-81 23.213046418554118 27.0556867440494 26.568663318588488 23.162603518807995 82-83 23.005390208047398 26.863901127043498 26.679866874499723 23.450841790409378 84-85 22.909381977503475 26.853036454302963 26.707472012381295 23.530109555812267 86-87 23.059389585481313 26.621323488653122 26.552698972295207 23.766587953570358 88-89 22.83854946175763 26.665225440959635 26.270107870201 24.22611722708174 90-91 23.1890549470043 26.484988247815867 26.351833784203286 23.97412302097654 92-93 23.105748166280854 26.13459986341342 26.69081322229022 24.0688387480155 94-95 23.06227203689538 26.18241482910389 26.719659427279076 24.035653706721654 96-97 22.903182891542222 26.28385494617576 26.762341880910412 24.050620281371604 98-99 22.8749380549418 26.614146865496906 26.118808127191635 24.392106952369655 100-101 22.651812413309845 26.44938181810118 26.44361633361754 24.455189434971434 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 663.0 1 433.0 2 230.5 3 264.5 4 233.5 5 229.5 6 371.0 7 672.0 8 943.5 9 1047.5 10 927.0 11 783.0 12 790.0 13 853.0 14 924.5 15 953.0 16 979.5 17 999.5 18 1020.0 19 1042.5 20 1070.0 21 1188.5 22 1340.0 23 1489.0 24 1656.0 25 1933.0 26 2361.0 27 2711.5 28 3023.5 29 3433.5 30 4123.5 31 4785.5 32 5264.5 33 5544.5 34 5866.5 35 6192.5 36 6527.0 37 7149.0 38 8214.0 39 8861.5 40 9150.5 41 10720.5 42 12406.0 43 13513.5 44 14546.0 45 15418.0 46 15661.5 47 16478.5 48 18884.5 49 19363.5 50 18128.0 51 17321.0 52 17187.5 53 17833.0 54 17655.5 55 16627.5 56 15167.0 57 13644.0 58 13191.0 59 12281.0 60 10190.0 61 8259.5 62 7043.0 63 5881.5 64 4458.5 65 3422.0 66 2556.5 67 2075.5 68 1486.5 69 878.0 70 666.0 71 536.0 72 457.0 73 353.0 74 264.0 75 219.0 76 149.5 77 75.0 78 39.0 79 27.0 80 11.5 81 4.0 82 2.5 83 3.0 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043680225274664364 2 0.0 3 0.0 4 0.0 5 0.019733705834746845 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033259054777663223 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.4345406370217626E-4 22-23 5.543175796277203E-4 24-25 2.2172703185108813E-4 26-27 0.0014412257070320728 28-29 0.0024389973503619695 30-31 2.2172703185108813E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.00709526501923482 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.1086351592554407E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.4345406370217626E-4 84-85 4.4345406370217626E-4 86-87 0.0 88-89 0.0 90-91 0.005543175796277203 92-93 0.001995543286659793 94-95 0.0 96-97 0.0 98-99 1.1086351592554407E-4 100-101 0.01008857994922451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 451005.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.822722208028594 #Duplication Level Percentage of deduplicated Percentage of total 1 68.89290891630488 33.63539354123769 2 15.667868608555413 15.29895993334785 3 6.384040437306091 9.350586985062499 4 3.084654149771641 6.024048506485738 5 1.7453381318080146 4.260607938417115 6 1.027892998860728 3.0110720605772867 7 0.6696201499781377 2.2884874997096745 8 0.47678349039520646 1.8622294323951547 9 0.34393767654291674 1.5112776274856654 >10 1.557872464971204 13.354762425262951 >50 0.09756500537863119 3.2687088662503063 >100 0.048782502686752674 4.149713271436937 >500 9.118224801262183E-4 0.3318791228725245 >1k 0.0018236449602524365 1.6522727894585845 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2525 0.5598607554239975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2271 0.5035420893338212 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1579 0.35010698329286816 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1048 0.23236992937994033 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 925 0.20509750446225652 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.12549750002771587 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 486 0.10775933747962883 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 469 0.10398997793816032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.2172703185108813E-4 0.0 22-23 0.0 0.0 0.0 2.2172703185108813E-4 0.0 24-25 0.0 0.0 0.0 2.2172703185108813E-4 0.0 26-27 0.0 0.0 0.0 4.4345406370217626E-4 0.0 28-29 0.0 0.0 0.0 0.0025498608662875137 0.0 30-31 0.0 0.0 0.0 0.007871309630713627 0.0 32-33 0.0 0.0 0.0 0.01419053003846964 0.0 34-35 0.0 0.0 0.0 0.02073147747807674 0.0 36-37 0.0 0.0 0.0 0.027494151949534928 0.0 38-39 0.0 0.0 0.0 0.03603064267580182 0.0 40-41 0.0 0.0 0.0 0.04234986308355783 0.0 42-43 0.0 0.0 0.0 0.04877994700723939 0.0 44-45 0.0 0.0 0.0 0.054101395771665506 0.0 46-47 0.0 0.0 0.0 0.06607465549162427 0.0 48-49 0.0 0.0 0.0 0.07882395982306183 0.0 50-51 0.0 0.0 0.0 0.08791476812895643 0.0 52-53 0.0 0.0 0.0 0.1119721510847995 0.0 54-55 0.0 0.0 0.0 0.1650757752131351 0.0 56-57 0.0 0.0 0.0 0.24944291083247416 0.0 58-59 0.0 0.0 0.0 0.3301515504262702 0.0 60-61 0.0 0.0 0.0 0.3891309408986596 0.0 62-63 0.0 0.0 0.0 0.47139166971541335 0.0 64-65 0.0 0.0 0.0 0.5805922329020743 0.0 66-67 0.0 0.0 0.0 0.7666212126251373 0.0 68-69 0.0 0.0 0.0 0.9914524229221405 0.0 70-71 0.0 0.0 0.0 1.1745989512311392 0.0 72-73 0.0 0.0 0.0 1.3768140042793318 0.0 74-75 0.0 0.0 0.0 1.6253700069844015 0.0 76-77 0.0 0.0 0.0 1.8863427234731323 0.0 78-79 0.0 0.0 0.0 2.1296881409297015 0.0 80-81 0.0 0.0 0.0 2.395871442666933 0.0 82-83 0.0 0.0 0.0 2.652853072582344 0.0 84-85 0.0 0.0 0.0 3.0711411181694217 0.0 86-87 0.0 0.0 0.0 3.560049223401071 0.0 88-89 0.0 0.0 0.0 4.170574605603042 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACGCA 25 0.0016142703 38.00652 86-87 TAGTACT 60 0.003949399 31.66508 4 GTGTAAG 95 3.474207E-5 30.015133 1 TAGGGAG 100 4.9482198E-5 28.49857 5 CGCGCTC 35 0.008392686 27.141495 58-59 GTACATG 1520 0.0 25.012611 1 GGTATCA 2670 0.0 24.562946 1 TACATGG 1595 0.0 23.525465 2 GTATCAA 3410 0.0 23.274197 1 ACATGGG 1635 0.0 22.078403 3 GAGTACT 2465 0.0 21.773754 12-13 AGTACTT 2455 0.0 20.991817 12-13 AGGTAAG 115 0.003720903 20.651138 2 ATAGCCT 115 0.003720903 20.651138 8 AGTACCC 115 0.003720903 20.651138 3 GTACTTT 2600 0.0 20.551853 14-15 AGAGTAC 3225 0.0 20.398203 10-11 TAGAGTG 140 4.8470945E-4 20.356121 7 CATGGGG 1050 0.0 19.903765 4 GTATGGA 170 7.909471E-5 19.56869 1 >>END_MODULE