FastQCFastQC Report
Thu 26 May 2016
SRR937743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425012
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42621.002795215193924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34440.8103300612688583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.5315144042991727No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.19222986645082962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.14540765907786132No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5880.13834903485078068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.10658522582891777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.050.9591261
GTATCAA32800.041.1816021
TATCAAC42050.032.111262
GGAGTCG600.00394581631.6705198
ATCAACG42600.031.5850663
GTACATG28050.031.5383421
TCAACGC42950.031.2169824
CAACGCA43350.030.8192625
AACGCAG44300.030.2406966
TACATGG29150.029.5219462
ACATGGG28050.028.9846613
GTATAGT1153.818117E-628.9506931
GTATACA1153.818117E-628.9506931
CATGGGG10750.027.863594
ACGCAGA48500.027.6219167
CGCAGAG48700.027.3133838
AGCGTAC350.00839020327.14295810-11
GTAAGGT1257.3224364E-626.6252064
ATGGGGG5450.026.1715685
TAAGGTG1658.3073246E-825.9336455
Produced by FastQC (version 0.11.5)