##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937743_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 425012 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.747280076797832 33.0 31.0 34.0 30.0 34.0 2 31.871605978184146 33.0 31.0 34.0 30.0 34.0 3 31.8659214328066 33.0 31.0 34.0 30.0 34.0 4 35.47497952998974 37.0 35.0 37.0 33.0 37.0 5 35.462290476504194 37.0 35.0 37.0 33.0 37.0 6 35.55019152400403 37.0 35.0 37.0 33.0 37.0 7 35.492480212323414 37.0 35.0 37.0 33.0 37.0 8 35.493609592199746 37.0 35.0 37.0 33.0 37.0 9 37.11000159995483 39.0 37.0 39.0 34.0 39.0 10-11 36.98831209471733 39.0 37.0 39.0 33.0 39.0 12-13 36.86588849256021 39.0 37.0 39.0 33.0 39.0 14-15 37.971964321007405 40.0 38.0 41.0 33.0 41.0 16-17 37.910164889461946 40.0 38.0 41.0 33.0 41.0 18-19 37.90949784947249 40.0 38.0 41.0 33.0 41.0 20-21 37.858489877932854 40.0 38.0 41.0 33.0 41.0 22-23 37.77735569819205 40.0 38.0 41.0 33.0 41.0 24-25 37.76671717504447 40.0 38.0 41.0 33.0 41.0 26-27 37.646751150555744 40.0 37.0 41.0 32.5 41.0 28-29 37.49794711678729 40.0 37.0 41.0 32.0 41.0 30-31 37.19848733682814 39.5 36.5 41.0 31.5 41.0 32-33 36.98484042803497 39.0 36.0 41.0 31.0 41.0 34-35 36.96205401259259 39.0 36.0 41.0 31.0 41.0 36-37 36.79795276368667 39.0 35.5 41.0 31.0 41.0 38-39 36.61534380205735 39.0 35.0 41.0 30.5 41.0 40-41 36.350371283634345 39.0 35.0 41.0 30.0 41.0 42-43 36.009215033928456 39.0 35.0 41.0 29.5 41.0 44-45 35.66407536728374 39.0 35.0 41.0 28.0 41.0 46-47 35.1512392591268 39.0 35.0 40.5 25.0 41.0 48-49 34.70362366239071 39.0 35.0 40.0 23.5 41.0 50-51 33.75189288773023 37.5 33.5 39.5 21.0 40.5 52-53 33.80051386784373 38.0 33.5 39.5 21.0 40.5 54-55 34.062979398228755 38.0 34.0 40.0 20.0 41.0 56-57 33.83742341392714 38.0 34.0 40.0 18.5 41.0 58-59 33.58325411988368 38.0 33.5 40.0 16.0 41.0 60-61 33.25632805661958 37.5 33.0 40.0 13.0 41.0 62-63 32.87173538629497 37.0 33.0 40.0 8.5 41.0 64-65 32.47792885847929 36.5 32.0 39.5 7.5 41.0 66-67 32.041387066718116 36.0 31.5 39.0 4.0 41.0 68-69 31.54329877744628 35.0 31.0 39.0 2.0 40.0 70-71 31.08630932773663 35.0 31.0 38.0 2.0 40.0 72-73 30.623320047433957 35.0 30.0 37.5 2.0 40.0 74-75 30.098777210996396 35.0 29.5 37.0 2.0 39.0 76-77 29.629004592811498 34.0 29.0 36.5 2.0 39.0 78-79 29.069479214704526 34.0 29.0 36.0 2.0 38.5 80-81 28.523961676376196 34.0 28.0 35.5 2.0 37.0 82-83 28.039143600651276 34.0 27.0 35.0 2.0 37.0 84-85 27.542219984376914 33.5 26.0 35.0 2.0 36.0 86-87 27.029819158047303 33.0 25.5 35.0 2.0 36.0 88-89 26.60391706587108 33.0 25.0 35.0 2.0 35.5 90-91 26.075399047556303 32.5 23.5 35.0 2.0 35.0 92-93 25.475404223880737 32.0 20.5 34.5 2.0 35.0 94-95 24.765018399480486 32.0 17.5 34.0 2.0 35.0 96-97 23.991130838658673 31.0 4.5 34.0 2.0 35.0 98-99 23.018350070115666 31.0 2.0 34.0 2.0 35.0 100-101 20.4195105079386 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.015709936566626226 1101 2 0.1273318457768653 1101 3 -0.0495708786843565 1101 4 -0.06345828287664546 1101 5 -0.07731839399708917 1101 6 0.1302990145024694 1101 7 0.010539588263476674 1101 8 0.10356715414653195 1101 9 0.05702800351380688 1101 10-11 0.10416877021409476 1101 12-13 -0.06990559550422404 1101 14-15 0.08422244877331764 1101 16-17 0.2774368292633298 1101 18-19 0.2674846757069247 1101 20-21 0.07930240655441878 1101 22-23 0.09243034294455299 1101 24-25 0.09772033384319201 1101 26-27 0.008532714361848548 1101 28-29 0.18358219161610378 1101 30-31 0.1528956216483337 1101 32-33 0.023794866412330862 1101 34-35 0.03853179727942546 1101 36-37 0.22304737792811835 1101 38-39 0.04108465952864293 1101 40-41 0.1347991311693093 1101 42-43 0.029569424791631604 1101 44-45 0.21429146924712228 1101 46-47 0.3186896884080923 1101 48-49 0.4088663962555117 1101 50-51 0.35101086430330497 1101 52-53 0.3656386500002924 1101 54-55 0.4935582287735514 1101 56-57 0.3906919345917501 1101 58-59 0.46973889739328456 1101 60-61 0.5237188505537773 1101 62-63 0.30658656094463765 1101 64-65 0.2409591604537411 1101 66-67 0.4671451766961283 1101 68-69 0.13263164555745277 1101 70-71 0.4153967818379023 1101 72-73 0.439174014245328 1101 74-75 0.4263163539927852 1101 76-77 0.151716831117362 1101 78-79 0.1698528081753956 1101 80-81 0.46504065232720393 1101 82-83 0.34349906211852144 1101 84-85 0.3669097124605649 1101 86-87 0.36818858179457337 1101 88-89 0.19860620758220193 1101 90-91 0.03578960015945043 1101 92-93 -0.044282208338284335 1101 94-95 0.2978259444099969 1101 96-97 0.2874338649731989 1101 98-99 0.1770438841252009 1101 100-101 0.30516605945011577 1102 1 0.022811446259648704 1102 2 0.08847783531126296 1102 3 0.1486128853231854 1102 4 -0.09314711479859739 1102 5 -0.11243809716062003 1102 6 -0.12704049117106564 1102 7 0.07895176619113897 1102 8 0.010823387152377961 1102 9 0.2534021684538317 1102 10-11 0.04367423634400325 1102 12-13 0.16915281150934192 1102 14-15 0.13826983226935852 1102 16-17 0.265503327103211 1102 18-19 0.3081752477476343 1102 20-21 0.26957060054343174 1102 22-23 0.22034825662719726 1102 24-25 0.42225040844638073 1102 26-27 0.23122799032311292 1102 28-29 0.20712823839397743 1102 30-31 0.3520002589922413 1102 32-33 0.09884552481602782 1102 34-35 0.24515327063529213 1102 36-37 0.5126371537452314 1102 38-39 0.5430088492534395 1102 40-41 0.39682337253768196 1102 42-43 0.3165106527471906 1102 44-45 0.5664596696656616 1102 46-47 0.6696285021821993 1102 48-49 0.7713347930855505 1102 50-51 0.6609899994705017 1102 52-53 0.7232745358534842 1102 54-55 0.5891076520869944 1102 56-57 0.6210627105519748 1102 58-59 0.5720989464235657 1102 60-61 0.5606025239258088 1102 62-63 0.631569813079345 1102 64-65 0.4046838027338566 1102 66-67 0.4314374834630925 1102 68-69 0.5294424994185896 1102 70-71 0.49958461925813324 1102 72-73 0.4833707492930621 1102 74-75 0.41971313218053297 1102 76-77 0.18474797696171308 1102 78-79 0.3272754828146702 1102 80-81 0.22607713259998263 1102 82-83 0.5237859431993428 1102 84-85 0.43636824308686073 1102 86-87 0.6571955526398057 1102 88-89 0.6780483325596478 1102 90-91 0.9309075196494021 1102 92-93 0.5893968673039929 1102 94-95 0.7261087852627597 1102 96-97 0.745732717407396 1102 98-99 0.733030494129121 1102 100-101 0.4296161798480824 1103 1 0.20815272170397847 1103 2 0.10879437442633844 1103 3 0.10768050153561504 1103 4 0.010324024361040074 1103 5 0.015674076656715386 1103 6 -0.01590820348339861 1103 7 -0.034405851605058047 1103 8 -0.026663627281401148 1103 9 0.1290252743059952 1103 10-11 0.17105358130775272 1103 12-13 0.15408284380212933 1103 14-15 0.09663481861942103 1103 16-17 0.06562291510844176 1103 18-19 -0.05561052463518479 1103 20-21 0.03315813332611839 1103 22-23 -0.05469341798068683 1103 24-25 0.08359019675815915 1103 26-27 0.10929117466580607 1103 28-29 -0.14254252549286406 1103 30-31 0.19118861748245308 1103 32-33 0.18047205590488602 1103 34-35 0.2845797313940466 1103 36-37 -0.019993862900449244 1103 38-39 0.04009363996772208 1103 40-41 0.11083087262492342 1103 42-43 0.22876916148073434 1103 44-45 -0.193447612720405 1103 46-47 0.01997236802888125 1103 48-49 0.038691159972422895 1103 50-51 -0.10029704126994687 1103 52-53 0.04732917321057073 1103 54-55 -0.23677033325105867 1103 56-57 0.032263583504153814 1103 58-59 0.10460044295135162 1103 60-61 0.30477680297522625 1103 62-63 0.04762534164221677 1103 64-65 -0.060727614900741855 1103 66-67 -0.09675696582810289 1103 68-69 0.20293637506317097 1103 70-71 0.017420143472353544 1103 72-73 -0.17192588533135478 1103 74-75 -0.22914037989503555 1103 76-77 -0.07514856800525394 1103 78-79 -0.009464073031988818 1103 80-81 0.1579435107879874 1103 82-83 0.1411244591269636 1103 84-85 0.06218260921674457 1103 86-87 0.07446239629492979 1103 88-89 0.2508815884940816 1103 90-91 0.3622410534145608 1103 92-93 -0.028040298661604623 1103 94-95 -0.13943591058642113 1103 96-97 0.1270352468749003 1103 98-99 0.012869781808735326 1103 100-101 0.11529031528451839 1104 1 0.2518056087172127 1104 2 0.17612204883007365 1104 3 0.18195794894274187 1104 4 0.1988990611280954 1104 5 0.249836267333869 1104 6 0.09056839348571799 1104 7 0.26440241618699645 1104 8 0.26234142419247775 1104 9 0.2833230235226978 1104 10-11 0.1745704302111264 1104 12-13 0.19574951046880074 1104 14-15 0.21771233316638927 1104 16-17 0.34129391389353003 1104 18-19 0.1726369656135489 1104 20-21 0.26183723684935956 1104 22-23 0.24180691771852736 1104 24-25 0.25459098005632796 1104 26-27 0.11899847692298948 1104 28-29 0.10279165508134014 1104 30-31 0.4098107320677826 1104 32-33 0.44423172723936233 1104 34-35 0.6368301150502944 1104 36-37 0.507553454702979 1104 38-39 0.38482076446417324 1104 40-41 0.31114139439741706 1104 42-43 0.3617679945263319 1104 44-45 0.4719661765244325 1104 46-47 0.7791721250465997 1104 48-49 0.9449611420684647 1104 50-51 0.7693217802659476 1104 52-53 1.009471094049502 1104 54-55 1.000209332968005 1104 56-57 1.0441629216971293 1104 58-59 0.9479468046113055 1104 60-61 1.209949767998566 1104 62-63 1.1557324776483568 1104 64-65 1.2721721069484886 1104 66-67 1.276750371597565 1104 68-69 1.2376412794688179 1104 70-71 1.1225119811857631 1104 72-73 1.1922957720636198 1104 74-75 1.3960714303230048 1104 76-77 1.0149986600783834 1104 78-79 0.8846288197360899 1104 80-81 1.0261835676969966 1104 82-83 0.9366376792817057 1104 84-85 0.931433760185481 1104 86-87 0.8385457775852956 1104 88-89 0.7695868285989498 1104 90-91 1.1900433425634809 1104 92-93 1.123917547108583 1104 94-95 1.2369748253941388 1104 96-97 1.1357414461203739 1104 98-99 1.1499809302197583 1104 100-101 0.8088528832236435 1105 1 0.2964243671517579 1105 2 0.15393918783142269 1105 3 0.2006826452990147 1105 4 0.1013956873089299 1105 5 0.19442128757678745 1105 6 0.3080953840390279 1105 7 0.17929782779293646 1105 8 0.2341700341789874 1105 9 0.3313156011205365 1105 10-11 0.5419793370937214 1105 12-13 0.28675828239862255 1105 14-15 0.43865531562252613 1105 16-17 0.38005066153186107 1105 18-19 0.35347367276605013 1105 20-21 0.25837907220698497 1105 22-23 0.5050913306739915 1105 24-25 0.45115811905767345 1105 26-27 0.46717661457480375 1105 28-29 0.27009091284112685 1105 30-31 0.45818286432149335 1105 32-33 0.4875853034876769 1105 34-35 0.633920196021954 1105 36-37 0.6530494061199832 1105 38-39 0.737806594423688 1105 40-41 0.9095303957469483 1105 42-43 0.8638344587638471 1105 44-45 0.6470252183597864 1105 46-47 0.8430216527119185 1105 48-49 0.5980903593424145 1105 50-51 0.5908889192673072 1105 52-53 0.43263236260550997 1105 54-55 0.9521324098910853 1105 56-57 1.0004297907929427 1105 58-59 0.8056138086598921 1105 60-61 0.9131380271078768 1105 62-63 0.8383404398615824 1105 64-65 0.6624808114019274 1105 66-67 0.9366272271981018 1105 68-69 0.9021149636793524 1105 70-71 0.7249495655312401 1105 72-73 0.8892424657208373 1105 74-75 0.8952279755321868 1105 76-77 0.9924784171634045 1105 78-79 1.151624433771179 1105 80-81 1.3744882235674432 1105 82-83 1.3567203378512076 1105 84-85 0.8503355820478262 1105 86-87 0.924325129812015 1105 88-89 1.081116855589503 1105 90-91 0.8968584572733356 1105 92-93 1.3823099222772761 1105 94-95 1.385507214058105 1105 96-97 1.5192181667681517 1105 98-99 1.2249218883844044 1105 100-101 1.3867881058957074 1106 1 -0.0632437151577605 1106 2 0.12167643440607279 1106 3 0.0029603135329985264 1106 4 0.3301800040084615 1106 5 -0.1658282194761469 1106 6 0.1677824258510512 1106 7 -0.1609051652805249 1106 8 0.22862753961403826 1106 9 0.10459787019379974 1106 10-11 -0.27640126859918723 1106 12-13 -0.17877018213447116 1106 14-15 -0.11943344012852464 1106 16-17 -0.06202805832253233 1106 18-19 0.05714612840080946 1106 20-21 -0.25090476418515095 1106 22-23 0.010324153364727806 1106 24-25 -0.10067716078458488 1106 26-27 -0.21185480764066966 1106 28-29 0.01736390439783264 1106 30-31 -0.18520469318899302 1106 32-33 -0.16291838729547692 1106 34-35 -0.10426803391889194 1106 36-37 -0.02879750221713806 1106 38-39 -0.2038929353806509 1106 40-41 -0.4597311948289189 1106 42-43 0.24424739672835472 1106 44-45 0.11484146286153418 1106 46-47 -0.5801070443910774 1106 48-49 -0.12854205382306105 1106 50-51 -0.00675567853844683 1106 52-53 -0.25743664998360316 1106 54-55 -0.07308372843744593 1106 56-57 -0.014048949284919843 1106 58-59 -0.049709387659433446 1106 60-61 -0.21161233944251023 1106 62-63 -0.49344453410069633 1106 64-65 0.17702274982146093 1106 66-67 0.10665920555899078 1106 68-69 -0.2822914774956331 1106 70-71 -0.0172643987743335 1106 72-73 0.2714217973351758 1106 74-75 0.10697717923307692 1106 76-77 0.24126500900323222 1106 78-79 0.39732694653724465 1106 80-81 0.07515875797261984 1106 82-83 0.19945126459168705 1106 84-85 0.33800242566984906 1106 86-87 0.12203798182677517 1106 88-89 0.017991192006917345 1106 90-91 -0.4952998924915697 1106 92-93 -0.1243575027694952 1106 94-95 -0.015265069677166565 1106 96-97 0.22966232471344483 1106 98-99 0.02385636434375371 1106 100-101 0.40555169353916654 1107 1 0.0038138852508069476 1107 2 0.2503471380868092 1107 3 0.3907781371933048 1107 4 0.21257157005376826 1107 5 -0.07081855252980773 1107 6 0.08430013642632161 1107 7 0.06984301988709518 1107 8 0.13243230377702986 1107 9 0.2863455811338653 1107 10-11 0.2251627703604342 1107 12-13 0.165158793169212 1107 14-15 0.28008047662629565 1107 16-17 0.13871155427003146 1107 18-19 0.48359525837563666 1107 20-21 -0.013134363280464356 1107 22-23 -0.04978874927271448 1107 24-25 0.3471441283847909 1107 26-27 0.23764907737737673 1107 28-29 -0.16188499211112628 1107 30-31 0.22290915790355115 1107 32-33 0.4995911884532518 1107 34-35 0.05239121534240354 1107 36-37 0.3566081447386793 1107 38-39 0.2965174538507327 1107 40-41 0.3201177819339307 1107 42-43 -0.04152030472715751 1107 44-45 -0.2748185524596636 1107 46-47 -0.46989473636202916 1107 48-49 -0.20424974725317924 1107 50-51 -0.15684870009756224 1107 52-53 -0.1094229338635131 1107 54-55 0.1736513972328524 1107 56-57 0.11174446989576126 1107 58-59 0.19586752840228883 1107 60-61 -0.11827158870380572 1107 62-63 -0.17076460122213177 1107 64-65 0.03814703392024654 1107 66-67 0.49190981658296096 1107 68-69 0.3869028828443497 1107 70-71 0.36055360728118657 1107 72-73 0.38210833292048463 1107 74-75 0.7501163832603659 1107 76-77 0.35257168831596886 1107 78-79 0.361290212141153 1107 80-81 0.994061285970286 1107 82-83 0.9865504511090322 1107 84-85 0.7706803522010581 1107 86-87 0.8389514900163242 1107 88-89 0.9096894738439971 1107 90-91 1.153853793715708 1107 92-93 1.228134927829128 1107 94-95 1.5861512291591495 1107 96-97 1.1436629813362167 1107 98-99 1.3231897098367646 1107 100-101 1.3873988098708345 1108 1 -0.02950117505019989 1108 2 -0.012261254218582707 1108 3 -0.10077473831750794 1108 4 0.009342226886218441 1108 5 -0.2876031633142233 1108 6 -0.037497929954618314 1108 7 0.0822022344789417 1108 8 0.04676500586015919 1108 9 0.16875861811183768 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74-75 -0.2690995098479334 2316 76-77 -0.26117655359682956 2316 78-79 -0.43412118026391155 2316 80-81 -0.4323100220465932 2316 82-83 -0.15080890191487484 2316 84-85 -0.3069743594726404 2316 86-87 -0.22384738480786837 2316 88-89 0.2557513585134785 2316 90-91 0.08106898358912318 2316 92-93 -0.2887427093016761 2316 94-95 0.08857738949670235 2316 96-97 0.21709828372721418 2316 98-99 0.033474519963348826 2316 100-101 0.11953664390740215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1831.0 3 363.0 4 352.0 5 357.0 6 381.0 7 493.0 8 634.0 9 736.0 10 1006.0 11 1329.0 12 1868.0 13 2548.0 14 2868.0 15 3745.0 16 5031.0 17 6910.0 18 5861.0 19 3610.0 20 3290.0 21 3360.0 22 3545.0 23 3965.0 24 4407.0 25 4893.0 26 5497.0 27 6397.0 28 7337.0 29 9129.0 30 10848.0 31 13298.0 32 16925.0 33 21662.0 34 29576.0 35 41828.0 36 58658.0 37 77640.0 38 56931.0 39 5903.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32443919716647 19.057142857142857 15.184415584415584 21.43400236127509 2 15.05300970507156 20.071466334352934 41.62347532192893 23.252048638646578 3 18.66839745102667 26.051687514751006 30.812603257021475 24.46731177720085 4 11.91933877431567 16.725828302234138 37.88075468848094 33.47407823496925 5 12.479021845605505 38.15864323565165 34.2991349093012 15.063200009441644 6 30.038055985211454 36.792403893311196 17.840334628588675 15.32920549288867 7 27.10941663012058 34.77796483087413 20.537015292769535 17.57560324623575 8 26.657172632661048 34.129480390261385 19.7429537448777 19.470393232199864 9 27.074265666335005 16.150625050103034 20.74878691709539 36.026322366466566 10-11 24.413991811270172 27.253168034009285 26.81862069534648 21.51421945937407 12-13 25.81839528394966 25.17513676758175 27.952217102401523 21.05425084606707 14-15 22.811552892997465 25.58047970840745 27.5817599053173 24.026207493277788 16-17 20.589871943297595 29.421563796099587 28.027464637628537 21.961099622974285 18-19 20.384848434836528 26.67674193251772 32.19071016478011 20.747699467865637 20-21 21.400744728376885 25.558045317865112 32.53006083480774 20.51114911895026 22-23 21.1860640829639 25.575545546808055 32.47077627777536 20.767614092452693 24-25 20.71308841523072 25.83156713479769 32.642177999806684 20.81316645016491 26-27 20.46439314643507 26.1677605212783 32.469801125915865 20.89804520637076 28-29 20.18207905418868 26.10023779684816 32.91240422398884 20.805278924974328 30-31 20.257759793622384 25.982191913261033 32.6827439356532 21.077304357463387 32-33 20.05770287382478 26.275087308904922 32.492943365348566 21.17426645192174 34-35 20.6247361955089 26.21880622425327 32.269390661733674 20.88706691850416 36-37 20.5799014638976 26.03415761061738 32.069611748897955 21.316329176587068 38-39 20.44494309456661 26.320987421524478 32.19231761917614 21.041751864732774 40-41 20.57853596903537 26.19334977111257 32.23293716085294 20.995177098999118 42-43 20.355993540249667 26.408001603149717 31.819457050911794 21.416547805688822 44-45 20.588939147984547 26.629933122787413 31.449147736984994 21.331979992243046 46-47 20.81602289928746 26.86764060945303 30.81406589675515 21.50227059450436 48-49 20.873013814584183 27.08156004121504 30.465296132906722 21.580130011294052 50-51 20.911994359867343 27.379873073436084 30.198406756841194 21.509725809855375 52-53 21.125024766253738 27.627463652574274 29.915393759847536 21.332117821324452 54-55 20.90934059781052 27.444658852874298 29.956538088406738 21.689462460908445 56-57 20.887276121469828 27.806370483684873 29.760273044300167 21.546080350545132 58-59 21.003308062267468 27.355094026985398 29.815104421395517 21.826493489351623 60-61 20.980388922015266 27.298545136589354 30.19000662265703 21.53105931873835 62-63 20.9046832241626 27.238903594266713 30.360699478384667 21.495713703186027 64-65 20.79577916262056 27.235690918175905 30.286535179930045 21.68199473927349 66-67 20.712200652169045 27.48903132744802 29.93071735059566 21.868050669787273 68-69 20.716026626186817 27.28995578016424 30.04438483514204 21.949632758506894 70-71 20.722917408141573 27.453938204008328 29.944940332385034 21.878204055465073 72-73 20.97965225220554 27.41607408437492 29.592149394375788 22.012124269043753 74-75 20.78942219980342 27.605027260063125 29.646173101990115 21.959377438143346 76-77 20.83689386072749 27.445507724330902 29.653722258826082 22.063876156115523 78-79 20.84786037071349 27.579801465786915 29.485701623492194 22.0866365400074 80-81 20.733825803288582 27.679514255543825 29.398287826218134 22.188372114949466 82-83 20.833451182260436 27.483430440849265 29.46293886940453 22.220179507485764 84-85 20.764451001580234 27.23079769363747 29.39634154245359 22.608409762328705 86-87 20.739075254448203 27.449178117866936 29.43915927508923 22.372587352595623 88-89 20.821519061929173 27.26686700842614 29.260621059454362 22.650992870190326 90-91 20.62042053579952 27.177488453002923 29.715529606158714 22.48656140503884 92-93 20.818720479678706 27.047213658775725 29.586196981267264 22.547868880278305 94-95 20.47277501349405 27.081324939129452 29.473366630446822 22.97253341692967 96-97 20.547601342074653 27.035591848086764 29.36477725779783 23.052029552040747 98-99 20.467483363937873 27.082778405059532 29.171273062426188 23.278465168576407 100-101 20.745253062018016 26.58579836338893 29.67544670998757 22.993501864605488 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 792.0 1 486.0 2 234.5 3 277.0 4 227.0 5 209.5 6 383.5 7 830.0 8 1396.0 9 1673.5 10 1426.0 11 1104.5 12 1084.0 13 1150.0 14 1169.0 15 1195.5 16 1222.5 17 1235.5 18 1257.5 19 1321.5 20 1428.0 21 1570.0 22 1787.0 23 2045.5 24 2314.0 25 2716.0 26 3360.5 27 4055.0 28 4410.5 29 4879.0 30 5652.0 31 6421.0 32 7360.0 33 8260.0 34 9215.5 35 9978.5 36 10975.0 37 12124.0 38 12399.0 39 12385.0 40 12369.0 41 13001.5 42 13605.5 43 14035.5 44 14962.0 45 15313.5 46 15013.0 47 15117.0 48 16203.5 49 15989.0 50 14544.5 51 13561.0 52 12762.0 53 12355.0 54 12013.0 55 11093.5 56 9926.5 57 8947.5 58 8091.5 59 7170.5 60 6111.5 61 5096.0 62 4069.5 63 3091.0 64 2330.5 65 1764.0 66 1315.5 67 994.5 68 699.5 69 459.0 70 330.5 71 245.5 72 199.5 73 151.5 74 113.5 75 92.5 76 65.0 77 52.0 78 39.0 79 25.5 80 17.0 81 12.5 82 10.0 83 10.5 84 7.5 85 2.5 86 3.5 87 3.0 88 1.0 89 1.5 90 3.0 91 2.0 92 1.5 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3557546610448646 2 0.30963831609460435 3 0.3086971661976603 4 0.3091677411461323 5 0.31928510253828124 6 0.21175872681241942 7 0.20940585207005918 8 0.2084647021731151 9 0.2084647021731151 10-11 0.20999407075564924 12-13 0.19846498451808417 14-15 0.20199429663162452 16-17 0.2124645892351275 18-19 0.2049353900595748 20-21 0.1954062473530159 22-23 0.19470038493030783 24-25 0.1983473407809662 26-27 0.1938768787704818 28-29 0.21422924529189763 30-31 0.21999378841068015 32-33 0.22187608820456836 34-35 0.21858206356526405 36-37 0.1887005543372893 38-39 0.1777596867853143 40-41 0.18493595474951297 42-43 0.19928849067791027 44-45 0.2062294711678729 46-47 0.21034700196700332 48-49 0.2105822894412393 50-51 0.2137586703434256 52-53 0.24658127299935062 54-55 0.1994061344150283 56-57 0.17846554920802235 58-59 0.17540681204295408 60-61 0.16728939418181135 62-63 0.17717146809972426 64-65 0.17317158103771188 66-67 0.17305393730059387 68-69 0.18070078021326458 70-71 0.17528916830583607 72-73 0.17387744346041994 74-75 0.17999491779055649 76-77 0.18834762312593525 78-79 0.1889358418115253 80-81 0.1778773305224323 82-83 0.17411273093465596 84-85 0.16646588802198525 86-87 0.16740703791892933 88-89 0.1734068685119479 90-91 0.18058313647614654 92-93 0.1712892812438237 94-95 0.1767008931512522 96-97 0.17493623709448203 98-99 0.1842300923268049 100-101 0.15517208925865622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 425012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.72169773148851 #Duplication Level Percentage of deduplicated Percentage of total 1 82.91145488313593 52.832586665411355 2 9.950162115085021 12.680824453535122 3 2.907651686687029 5.558415056625709 4 1.2641538750100552 3.2221612443792265 5 0.7272571869762201 2.3171031320775657 6 0.46552902615230995 1.7798579933827026 7 0.3109103588673283 1.386821513653276 8 0.228523003038395 1.164949897944371 9 0.1728502801956365 0.9912881976686501 >10 0.9909469653079814 11.26513085408325 >50 0.05098097119721934 2.2912004169907743 >100 0.017363084383158887 1.6876210605409792 >500 0.0011082819819037586 0.4762250530721719 >1k 0.0011082819819037586 2.3458144606347964 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4262 1.002795215193924 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3444 0.8103300612688583 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2259 0.5315144042991727 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 817 0.19222986645082962 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 618 0.14540765907786132 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 588 0.13834903485078068 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 453 0.10658522582891777 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.3528747423602156E-4 0.0 0.0 0.0 0.0 8 2.3528747423602156E-4 0.0 0.0 0.0 0.0 9 2.3528747423602156E-4 0.0 0.0 0.0 0.0 10-11 2.3528747423602156E-4 0.0 0.0 0.0 0.0 12-13 2.3528747423602156E-4 0.0 0.0 0.0 0.0 14-15 2.3528747423602156E-4 0.0 0.0 0.0 0.0 16-17 2.3528747423602156E-4 0.0 0.0 0.0 0.0 18-19 2.3528747423602156E-4 0.0 0.0 1.1764373711801078E-4 0.0 20-21 2.3528747423602156E-4 0.0 0.0 2.3528747423602156E-4 0.0 22-23 2.3528747423602156E-4 0.0 0.0 3.529312113540323E-4 0.0 24-25 2.3528747423602156E-4 0.0 0.0 7.058624227080646E-4 0.0 26-27 2.3528747423602156E-4 0.0 0.0 0.0014117248454161295 0.0 28-29 2.3528747423602156E-4 0.0 0.0 0.002705805953714248 0.0 30-31 2.3528747423602156E-4 0.0 0.0 0.005529255644546507 0.0 32-33 2.3528747423602156E-4 0.0 0.0 0.007764486649788712 0.0 34-35 3.529312113540323E-4 0.0 0.0 0.009529142706558875 0.0 36-37 4.705749484720431E-4 0.0 0.0 0.012823167345863175 0.0 38-39 7.058624227080648E-4 0.0 0.0 0.016940698144993552 0.0 40-41 7.058624227080648E-4 0.0 0.0 0.026587484588670437 0.0 42-43 7.058624227080648E-4 0.0 0.0 0.042351745362483886 0.0 44-45 7.058624227080648E-4 0.0 0.0 0.061645318249837655 0.0 46-47 7.058624227080648E-4 0.0 0.0 0.10235005129266939 0.0 48-49 7.058624227080648E-4 0.0 0.0 0.14564294655209736 0.0 50-51 7.058624227080648E-4 0.0 0.0 0.17740675557396027 0.0 52-53 7.058624227080648E-4 0.0 0.0 0.22093493830762426 0.0 54-55 7.058624227080648E-4 0.0 0.0 0.2670512832578845 0.0 56-57 7.058624227080648E-4 0.0 0.0 0.3650485162771875 0.0 58-59 7.058624227080648E-4 0.0 0.0 0.4665750614100308 0.0 60-61 7.058624227080648E-4 0.0 0.0 0.5730426435018305 0.0 62-63 7.058624227080648E-4 0.0 0.0 0.6892746557744251 0.0 64-65 7.058624227080648E-4 0.0 0.0 0.8093889113719142 0.0 66-67 7.058624227080648E-4 0.0 0.0 0.9693843938524089 0.0 68-69 7.058624227080648E-4 0.0 0.0 1.178202027236878 0.0 70-71 8.235061598260754E-4 0.0 0.0 1.3666672940999312 0.0 72-73 9.411498969440862E-4 0.0 0.0 1.5285450763743142 0.0 74-75 9.411498969440862E-4 0.0 0.0 1.7267747734181622 0.0 76-77 9.411498969440862E-4 0.0 0.0 1.9684150094585564 0.0 78-79 9.411498969440862E-4 0.0 0.0 2.22546657506141 0.0 80-81 0.001176437371180108 0.0 0.0 2.564280537961281 0.0 82-83 0.001176437371180108 0.0 0.0 2.908859043979935 0.0 84-85 0.001176437371180108 0.0 0.0 3.2852013590204514 0.0 86-87 0.001176437371180108 0.0 0.0 3.6603672366897877 0.0 88-89 0.001176437371180108 0.0 0.0 4.200116702587222 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1820 0.0 50.959126 1 GTATCAA 3280 0.0 41.181602 1 TATCAAC 4205 0.0 32.11126 2 GGAGTCG 60 0.003945816 31.670519 8 ATCAACG 4260 0.0 31.585066 3 GTACATG 2805 0.0 31.538342 1 TCAACGC 4295 0.0 31.216982 4 CAACGCA 4335 0.0 30.819262 5 AACGCAG 4430 0.0 30.240696 6 TACATGG 2915 0.0 29.521946 2 ACATGGG 2805 0.0 28.984661 3 GTATAGT 115 3.818117E-6 28.950693 1 GTATACA 115 3.818117E-6 28.950693 1 CATGGGG 1075 0.0 27.86359 4 ACGCAGA 4850 0.0 27.621916 7 CGCAGAG 4870 0.0 27.313383 8 AGCGTAC 35 0.008390203 27.142958 10-11 GTAAGGT 125 7.3224364E-6 26.625206 4 ATGGGGG 545 0.0 26.171568 5 TAAGGTG 165 8.3073246E-8 25.933645 5 >>END_MODULE