FastQCFastQC Report
Thu 26 May 2016
SRR937743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425012
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42641.003265790142396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38990.9173858620462482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.6093945582712958No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9100.21411160155477962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.16493651943945112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.14140777201584895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.041.618041
TTTTACG500.001615288837.9970362
GTATCAA33550.036.8078581
GGTAAGG1605.2750693E-1132.65373
TAGCACC902.4106974E-531.6641964
GTACACG600.00394968431.6641941
TAAGGTG2850.029.997665
GTAAGGT2250.029.553254
TAGGACG650.00584221129.2284874
ATCAACG43900.028.0217343
TCAACGC43900.028.0217344
CATTCCG856.403407E-427.9389959
TATCAAC44300.027.7687132
CAACGCA44350.027.7374085
AACGCAG45200.027.2157976
CGGTTAC350.00839355727.1407428-29
AGGTAAG1806.679329E-926.386832
ATGGGAG7350.025.8483265
GTACATG29000.025.222171
GTAAGGC950.001225840124.9980493
Produced by FastQC (version 0.11.5)