FastQCFastQC Report
Thu 26 May 2016
SRR937742_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937742_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354962
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.9826403953099204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.7950146776274644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.5327330812875744No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.1842450741206101No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.14339563108163692No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4830.13607090336430377No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4220.11888596525825298No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCCG405.381563E-447.502757
GGTATCA14700.047.266371
GTATCAA26200.039.9611631
ATCAACG33300.031.1374723
TATCAAC33800.030.6811942
TCAACGC34050.030.4559274
CAACGCA34800.029.7995515
GTCTAGG804.4378842E-429.7438181
CATTCCG804.4859102E-429.6892199
AACGCAG35450.029.2118476
GTACATG23950.028.0175611
TAAGGTG1703.5670382E-927.9823075
ATATAGG856.3961925E-427.9427956
GTAAGGT1205.306809E-627.7491254
TACATGG24450.027.2384012
ACATGGG24350.026.9557343
ACGCAGA38800.026.567267
CGCAGAG38700.026.5131658
GTATAGG1109.337344E-525.9582421
GCAGAGT39300.025.2622819
Produced by FastQC (version 0.11.5)