##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937742_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 354962 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75825863050129 33.0 31.0 34.0 30.0 34.0 2 31.879908271871354 33.0 31.0 34.0 30.0 34.0 3 31.891461621243963 33.0 31.0 34.0 30.0 34.0 4 35.47809061251627 37.0 35.0 37.0 33.0 37.0 5 35.46523853257532 37.0 35.0 37.0 33.0 37.0 6 35.57068362247227 37.0 35.0 37.0 33.0 37.0 7 35.513238036747595 37.0 35.0 37.0 33.0 37.0 8 35.50575836286701 37.0 35.0 37.0 33.0 37.0 9 37.12047486773232 39.0 37.0 39.0 34.0 39.0 10-11 37.01645105673283 39.0 37.0 39.0 33.0 39.0 12-13 36.88574692502296 39.0 37.0 39.0 33.0 39.0 14-15 37.98302212631211 40.0 38.0 41.0 33.0 41.0 16-17 37.94854660498871 40.0 38.0 41.0 33.0 41.0 18-19 37.95825609501862 40.0 38.0 41.0 33.0 41.0 20-21 37.75119308545703 40.0 38.0 41.0 32.5 41.0 22-23 37.686557997757504 40.0 37.5 41.0 32.5 41.0 24-25 37.54683177354195 40.0 37.0 41.0 32.0 41.0 26-27 37.600434694418 40.0 37.0 41.0 32.0 41.0 28-29 37.5160284762876 40.0 37.0 41.0 32.0 41.0 30-31 37.246588367205504 39.5 36.5 41.0 32.0 41.0 32-33 36.952234323674084 39.0 35.5 41.0 31.0 41.0 34-35 36.651416207932115 39.0 35.0 41.0 30.5 41.0 36-37 36.64862435979063 39.0 35.0 41.0 31.0 41.0 38-39 36.58133828409802 39.0 35.0 41.0 30.0 41.0 40-41 36.34787385691989 39.0 35.0 41.0 30.0 41.0 42-43 36.04970109476507 39.0 35.0 41.0 30.0 41.0 44-45 35.69993548605203 39.0 35.0 41.0 28.0 41.0 46-47 35.1729044235721 39.0 35.0 40.5 25.0 41.0 48-49 34.72136031462523 39.0 35.0 40.0 23.5 41.0 50-51 33.763973326722294 38.0 33.5 39.5 21.0 40.5 52-53 33.83338498205442 38.0 33.5 39.5 21.0 40.5 54-55 34.12776156320958 38.0 34.0 40.0 20.0 41.0 56-57 33.89221663163944 38.0 34.0 40.0 18.5 41.0 58-59 33.6433364698193 38.0 34.0 40.0 16.5 41.0 60-61 33.32598700705991 38.0 33.0 40.0 13.5 41.0 62-63 32.95936325578512 37.0 33.0 40.0 9.0 41.0 64-65 32.57869997351829 36.5 32.0 39.5 7.5 41.0 66-67 32.16006079524006 36.0 32.0 39.0 4.0 41.0 68-69 31.69772539032347 35.5 31.0 39.0 2.0 40.0 70-71 31.249049193998232 35.0 31.0 38.5 2.0 40.0 72-73 30.79668809619058 35.0 30.5 37.5 2.0 40.0 74-75 30.305603134983464 35.0 30.0 37.0 2.0 39.0 76-77 29.861731678320496 34.5 29.5 36.5 2.0 39.0 78-79 29.33876583972369 34.0 29.0 36.0 2.0 38.5 80-81 28.816832506014727 34.0 29.0 35.5 2.0 37.5 82-83 28.34175630067444 34.0 27.5 35.0 2.0 37.0 84-85 27.89577194178532 34.0 27.0 35.0 2.0 36.5 86-87 27.426775260450416 33.5 26.0 35.0 2.0 36.0 88-89 26.945161735622406 33.0 25.5 35.0 2.0 36.0 90-91 26.43921039435207 33.0 24.5 35.0 2.0 35.0 92-93 25.910490418692703 32.5 23.5 35.0 2.0 35.0 94-95 25.308148477865238 32.0 19.5 34.5 2.0 35.0 96-97 24.591068903150195 32.0 12.5 34.0 2.0 35.0 98-99 23.72732996771485 31.5 2.0 34.0 2.0 35.0 100-101 21.342908536688434 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0653089638169213 1101 2 0.2895644672813873 1101 3 0.3374397582710422 1101 4 0.007920814002105203 1101 5 0.23182529435342047 1101 6 0.1978571856505198 1101 7 0.1516737821175198 1101 8 0.14438052016614478 1101 9 0.2760229813604269 1101 10-11 0.06013975488605894 1101 12-13 0.1620082151501876 1101 14-15 0.3587406791433523 1101 16-17 0.42485564681953036 1101 18-19 0.19640589890710203 1101 20-21 0.34389889550504193 1101 22-23 0.20867647869395256 1101 24-25 0.2580693481376457 1101 26-27 0.31210934137074275 1101 28-29 0.2015925613890701 1101 30-31 0.3788826021464473 1101 32-33 0.44921376105510546 1101 34-35 0.38309580038921354 1101 36-37 0.4784752077560199 1101 38-39 0.43478920249607 1101 40-41 0.42096982473164246 1101 42-43 0.37028546866158507 1101 44-45 0.43786420393454506 1101 46-47 0.6058853903877264 1101 48-49 0.9278232624555471 1101 50-51 0.8560121318392646 1101 52-53 0.894712330371263 1101 54-55 1.0333256136188211 1101 56-57 1.1339900070098778 1101 58-59 1.1084387182973714 1101 60-61 1.062033090024613 1101 62-63 1.0807878991963094 1101 64-65 1.2324793056986678 1101 66-67 1.2045387371827339 1101 68-69 1.254824305959442 1101 70-71 0.9053044287906644 1101 72-73 1.1755498605400163 1101 74-75 0.8445900566019944 1101 76-77 0.9230962127053317 1101 78-79 0.8554038521501717 1101 80-81 1.0207717768456703 1101 82-83 1.2188242580814936 1101 84-85 0.9335049740006944 1101 86-87 1.199053924838335 1101 88-89 1.201104426628305 1101 90-91 0.9940607990353207 1101 92-93 1.290498040356585 1101 94-95 1.466359832264331 1101 96-97 1.2668192147651531 1101 98-99 1.4263209795764702 1101 100-101 1.558807236757609 1102 1 -0.027017234274879343 1102 2 0.15176682382475803 1102 3 0.1372866649661404 1102 4 -0.0725160486786649 1102 5 0.021939840951198164 1102 6 0.024148226958374153 1102 7 -0.027959340712520486 1102 8 0.16154063936021856 1102 9 0.10980192556338153 1102 10-11 0.06779852084466853 1102 12-13 0.0917657262904612 1102 14-15 0.04693472490731665 1102 16-17 0.17060730760851328 1102 18-19 0.1110222088116899 1102 20-21 0.3795792038786985 1102 22-23 0.4131599254803575 1102 24-25 0.39111699842879943 1102 26-27 0.3226891822630549 1102 28-29 0.4125906750608479 1102 30-31 0.23377748718977642 1102 32-33 0.3019503038802256 1102 34-35 0.24708333422010043 1102 36-37 0.24377086920111424 1102 38-39 0.5556303955303221 1102 40-41 0.5706280986046295 1102 42-43 0.647227156515271 1102 44-45 0.6521784279428502 1102 46-47 0.8288685227860952 1102 48-49 0.8249008206173372 1102 50-51 0.706784432886316 1102 52-53 0.759900006360219 1102 54-55 0.7875534492622265 1102 56-57 0.9110424141287226 1102 58-59 0.8165143901309406 1102 60-61 0.9877582328661347 1102 62-63 0.7603390077558672 1102 64-65 0.9722370902198421 1102 66-67 1.0969824890622313 1102 68-69 0.9897774211348143 1102 70-71 1.2248744006324337 1102 72-73 1.105847298960967 1102 74-75 1.1004349402784221 1102 76-77 1.0389523049987268 1102 78-79 1.2814898577818177 1102 80-81 1.4304453600491485 1102 82-83 1.2344930844572808 1102 84-85 1.2915642429801615 1102 86-87 1.261797575566895 1102 88-89 1.1461480308821095 1102 90-91 1.1386232529022955 1102 92-93 1.379290790295073 1102 94-95 1.6100884743813957 1102 96-97 1.3922641421825226 1102 98-99 1.1052569811074058 1102 100-101 1.320278299389173 1103 1 5.319805006145373E-4 1103 2 0.12499177405700479 1103 3 0.07690179436229272 1103 4 0.07108050075729722 1103 5 0.006135815293156099 1103 6 0.0782623609995099 1103 7 0.10690020363607289 1103 8 0.18382553208306263 1103 9 0.0075347280407100925 1103 10-11 0.207220108988885 1103 12-13 0.08286282870142969 1103 14-15 0.20657860900375624 1103 16-17 0.13659381501837942 1103 18-19 -0.006794645866477822 1103 20-21 0.01704326050333549 1103 22-23 0.12399823558874346 1103 24-25 7.830015254555178E-4 1103 26-27 -0.0330728172945598 1103 28-29 -0.05167608008181901 1103 30-31 -0.14272107507520815 1103 32-33 -0.10276104316688617 1103 34-35 -0.08995493165028279 1103 36-37 0.008684605100249598 1103 38-39 0.10068126923083298 1103 40-41 -0.047492892326580716 1103 42-43 0.1488860815318631 1103 44-45 0.026194132433012385 1103 46-47 -0.06797398993232662 1103 48-49 0.30477253041605223 1103 50-51 0.1231469632999378 1103 52-53 0.11517538791297 1103 54-55 0.12143422785103297 1103 56-57 0.4173573886918689 1103 58-59 0.4836344963021375 1103 60-61 0.5466943089554945 1103 62-63 0.24919103164438638 1103 64-65 0.5547299015447749 1103 66-67 0.43170931940949586 1103 68-69 0.7442012211790505 1103 70-71 0.7192727638264138 1103 72-73 0.8421058700735529 1103 74-75 0.886903641893106 1103 76-77 0.7400693145098955 1103 78-79 0.7357920033248391 1103 80-81 0.8327567955722621 1103 82-83 0.6689434273017874 1103 84-85 0.9423660567381837 1103 86-87 1.1924655693735744 1103 88-89 1.2798242087188711 1103 90-91 1.062821439144276 1103 92-93 1.1250826505029927 1103 94-95 0.938738109417887 1103 96-97 1.2751440360113193 1103 98-99 0.8817942516227752 1103 100-101 1.1327091775134832 1104 1 -0.13084742440820207 1104 2 -0.1340717114770662 1104 3 -0.2340127812581656 1104 4 -0.2923638979812182 1104 5 -0.2921159329585947 1104 6 -0.09484198563424684 1104 7 -0.1814036493736637 1104 8 -0.05966137316854514 1104 9 0.09077207892512007 1104 10-11 0.06993435552666227 1104 12-13 -0.09408767177848176 1104 14-15 -0.22558236727486758 1104 16-17 -0.24324644276305918 1104 18-19 -0.16455551245087463 1104 20-21 -0.4330013027765247 1104 22-23 -0.25185532261775734 1104 24-25 -0.2704908226895455 1104 26-27 -0.5250582828094394 1104 28-29 -0.30262219279264 1104 30-31 -0.15325172089997352 1104 32-33 -0.20445444780147426 1104 34-35 -0.19107929748052754 1104 36-37 -0.13402415672615575 1104 38-39 0.024371794391946366 1104 40-41 0.14184277200908468 1104 42-43 -0.2155729888095621 1104 44-45 -0.35078036365913334 1104 46-47 -0.29320709016542423 1104 48-49 -0.23979074944134737 1104 50-51 0.07167637506464786 1104 52-53 -0.24261290222825949 1104 54-55 -0.668799557970992 1104 56-57 -0.19246024465517308 1104 58-59 0.16104434135904455 1104 60-61 0.31282621626387197 1104 62-63 -0.018401180065467315 1104 64-65 -0.4949547275516224 1104 66-67 -0.2871512318039926 1104 68-69 -0.04971074920350915 1104 70-71 -0.3859000045816465 1104 72-73 -0.11705016996484119 1104 74-75 -0.333530335893812 1104 76-77 -0.018123473094657783 1104 78-79 -0.19454530642423506 1104 80-81 -0.21596115314568465 1104 82-83 -0.003955242655710123 1104 84-85 0.20870706866742594 1104 86-87 0.2267930066421897 1104 88-89 0.27889180778647216 1104 90-91 0.429248149688636 1104 92-93 0.019752196643128883 1104 94-95 -0.1619029162231378 1104 96-97 -1.5419516926229448E-4 1104 98-99 0.24406972125118997 1104 100-101 -0.017640472836166765 1105 1 -0.21878252376534846 1105 2 0.027301276851947165 1105 3 0.04924660165855599 1105 4 -0.08993744569753659 1105 5 0.1588424937279882 1105 6 0.33029236982563503 1105 7 0.23155451372727498 1105 8 0.2292065264931864 1105 9 0.13276273627842983 1105 10-11 -0.032607781567158156 1105 12-13 -0.09601331133975322 1105 14-15 -0.06636931685679315 1105 16-17 -0.01117758833554916 1105 18-19 0.4120344215062772 1105 20-21 0.2974860377907689 1105 22-23 0.0013003832792435333 1105 24-25 0.18438111861872386 1105 26-27 0.13619755634474728 1105 28-29 0.031304208238275066 1105 30-31 0.22337189344494135 1105 32-33 -0.36988372630899136 1105 34-35 -0.1698313658956465 1105 36-37 -0.20794528604420748 1105 38-39 -0.33167383521164595 1105 40-41 0.014135527796987901 1105 42-43 -0.30595357719128913 1105 44-45 -0.20507241655198527 1105 46-47 0.2598797905913557 1105 48-49 0.19657894406712728 1105 50-51 -0.06657795579391035 1105 52-53 -0.14859336760335395 1105 54-55 0.1116445838380784 1105 56-57 -0.1177117492898887 1105 58-59 0.08684132183906002 1105 60-61 -0.08394705615189224 1105 62-63 0.004516127260743019 1105 64-65 -0.11893882455113669 1105 66-67 0.1978317083474188 1105 68-69 0.04774638152033006 1105 70-71 0.053760848557217855 1105 72-73 -0.20500357388055335 1105 74-75 -0.07253295710659913 1105 76-77 -0.21535875256865822 1105 78-79 -0.40516121821091033 1105 80-81 -0.18471893258308114 1105 82-83 -0.05056230967968034 1105 84-85 0.04159377077172266 1105 86-87 0.14128875266277774 1105 88-89 -0.4812525785715067 1105 90-91 -0.4746748833035035 1105 92-93 0.2710005675374134 1105 94-95 -0.4768767773135032 1105 96-97 -0.41204337204987596 1105 98-99 -0.3579159572621897 1105 100-101 -0.0031396015391784715 1106 1 -0.11300033839651391 1106 2 -0.1294691441378184 1106 3 -0.1409418784170029 1106 4 0.20551930944503738 1106 5 0.06460245594863778 1106 6 -0.04761226508970395 1106 7 0.09060419635294181 1106 8 -8.863940451675489E-4 1106 9 0.005993565340752127 1106 10-11 -0.479140275976647 1106 12-13 -0.532496775928557 1106 14-15 -0.41186982382235726 1106 16-17 -0.258389277711494 1106 18-19 -0.1848932282569109 1106 20-21 -0.24359206268563582 1106 22-23 -0.29908018813345905 1106 24-25 -0.319473208644915 1106 26-27 -0.10806956589645722 1106 28-29 0.1094046846470107 1106 30-31 -0.011839295910171188 1106 32-33 0.15618680440756805 1106 34-35 0.03223260899219582 1106 36-37 0.03228000112054019 1106 38-39 -0.0011947874735511732 1106 40-41 -0.5025943359473359 1106 42-43 -0.5553667471844861 1106 44-45 -0.7240107056473661 1106 46-47 -0.7025005863687994 1106 48-49 -0.5269025898856725 1106 50-51 -0.28082299378160513 1106 52-53 -0.26433430125337054 1106 54-55 -0.3296029681432415 1106 56-57 0.003168287378308321 1106 58-59 -0.4801059120734621 1106 60-61 -0.4496706279673859 1106 62-63 -0.08039018828017674 1106 64-65 0.12771728612013789 1106 66-67 -0.18969138289249443 1106 68-69 -0.07836998443936594 1106 70-71 -0.5217631316086226 1106 72-73 -0.5201486493698084 1106 74-75 -0.679863818114697 1106 76-77 -0.43284003651104186 1106 78-79 -0.8869937945682551 1106 80-81 -1.1327653789627874 1106 82-83 -1.3910903872384672 1106 84-85 -0.9301758862973237 1106 86-87 -1.0688102792413297 1106 88-89 -0.4097843229497471 1106 90-91 -0.4661745291220747 1106 92-93 -0.231631486575953 1106 94-95 -0.4384793440871171 1106 96-97 -0.3812156986745947 1106 98-99 0.06985186639712992 1106 100-101 0.2734622013137198 1107 1 -0.05098317101453986 1107 2 0.030294804360035954 1107 3 0.0981139155744124 1107 4 -0.02304828712149032 1107 5 0.18624945165679208 1107 6 0.17011842160557222 1107 7 0.27716535515122587 1107 8 0.30238613814367454 1107 9 -0.14076072650474458 1107 10-11 0.22123526050403797 1107 12-13 0.38409388930749344 1107 14-15 0.3948361847613313 1107 16-17 0.3148135119880706 1107 18-19 0.2603455056486723 1107 20-21 0.043653108988188194 1107 22-23 0.26348687195238085 1107 24-25 0.022184516676979626 1107 26-27 0.048555970584224895 1107 28-29 0.19235801082727022 1107 30-31 0.0768811976520638 1107 32-33 0.07091319925735462 1107 34-35 0.37926855319820163 1107 36-37 0.14504824146388273 1107 38-39 0.2914956631702239 1107 40-41 0.2238139258552323 1107 42-43 0.581556536077322 1107 44-45 -0.26016950393062643 1107 46-47 -0.2746974747808082 1107 48-49 -0.14787898473546335 1107 50-51 -0.08935303670006789 1107 52-53 0.014796600034188145 1107 54-55 0.1849758945176987 1107 56-57 -0.11067291433842996 1107 58-59 0.004751920544563859 1107 60-61 -0.21419584255965418 1107 62-63 -0.009521089567726904 1107 64-65 -0.10786479542492344 1107 66-67 0.21665250122768143 1107 68-69 -0.06346923336640131 1107 70-71 -0.13328405435540347 1107 72-73 0.01757935492203444 1107 74-75 -0.5352554490159847 1107 76-77 -0.6226200794294989 1107 78-79 -0.46875345122061773 1107 80-81 0.16504846223892855 1107 82-83 0.04687903336239074 1107 84-85 0.33810469310181546 1107 86-87 0.538204205737209 1107 88-89 0.584180269324932 1107 90-91 0.6847911361139758 1107 92-93 0.4960106808060196 1107 94-95 0.6679047760845549 1107 96-97 0.3404849451022294 1107 98-99 0.5998813728348971 1107 100-101 1.0273114502407594 1108 1 0.0035622835309148115 1108 2 0.1371129861782201 1108 3 -0.12574972078922286 1108 4 -0.25202555984876085 1108 5 0.07545399711133882 1108 6 0.017277512514667137 1108 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0.8274451081215837 2316 86-87 0.7906869132470931 2316 88-89 0.8076239452142602 2316 90-91 0.8560361954025133 2316 92-93 0.6863145345609638 2316 94-95 0.319930467784161 2316 96-97 0.23265391743424502 2316 98-99 0.5210893767874687 2316 100-101 0.5269779522170417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1548.0 3 269.0 4 271.0 5 306.0 6 329.0 7 423.0 8 501.0 9 598.0 10 794.0 11 1079.0 12 1474.0 13 2024.0 14 2505.0 15 3022.0 16 4206.0 17 5823.0 18 4889.0 19 2868.0 20 2781.0 21 2766.0 22 2903.0 23 3124.0 24 3528.0 25 3860.0 26 4431.0 27 5132.0 28 5998.0 29 7149.0 30 8600.0 31 10822.0 32 13529.0 33 17706.0 34 24018.0 35 34701.0 36 48377.0 37 65931.0 38 50378.0 39 6298.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10859047839868 19.209747242400166 15.162062891090006 21.519599388111146 2 15.079708240427522 20.17504443935759 41.37452557983117 23.37072174038372 3 18.534604559686223 25.916119406359144 30.903912016366945 24.645364017587685 4 11.90295185745171 16.816402662182593 37.873080020913115 33.407565459452584 5 12.50370931412309 38.243664491477766 34.258518704834685 14.994107489564461 6 30.005788262533002 36.79330255671792 17.782389564186186 15.4185196165629 7 27.1086242137477 34.75828035187967 20.47502625546283 17.658069178909805 8 26.507132995858264 34.178526760380464 19.72281118806095 19.591529055700327 9 26.89131152956723 16.240163316476497 20.742660782303993 36.125864371652284 10-11 24.41299044521244 27.187662352329966 26.802816742336972 21.596530460120622 12-13 25.768819761967617 25.14599987859812 27.95871206720582 21.126468292228445 14-15 22.666531143504912 25.523528797260177 27.620608555464266 24.18933150377065 16-17 20.58748389154795 29.336508209841085 27.967942592511562 22.1080653060994 18-19 20.32085259554646 26.76599498888379 32.26904753502488 20.64410488054487 20-21 21.48804071534122 25.601633807508232 32.51751163325707 20.392813843893475 22-23 21.05176981719582 25.621098067224402 32.41312531494235 20.914006800637434 24-25 20.653774113548756 25.824769115461326 32.7417777156722 20.77967905531772 26-27 20.349861387927483 26.089699796175324 32.52076358031314 21.039675235584063 28-29 20.158343827470734 26.114872686819172 32.88020306861754 20.84658041709255 30-31 20.168387528481038 26.06773899368406 32.67152856009464 21.092344917740263 32-33 19.961852645607973 26.272966768600014 32.45631125530491 21.308869330487106 34-35 20.541308208561208 26.263414826966024 32.338309160057484 20.856967804415284 36-37 20.556009558046217 25.953511085893975 32.149445387734595 21.34103396832521 38-39 20.313657640977297 26.39643695306425 32.16550217539482 21.124403230563626 40-41 20.498907748494275 26.26370959092782 32.142957941261145 21.094424719316766 42-43 20.220805139887478 26.41785862486556 31.954218642819033 21.407117592427923 44-45 20.443260058886796 26.579512701876418 31.472960345981104 21.504266893255682 46-47 20.761494759871553 26.791347612830375 30.88916334380183 21.557994283496242 48-49 20.671078785688472 27.066627891217927 30.514631143838088 21.74766217925551 50-51 20.799835114650374 27.346545807211193 30.19844121230259 21.65517786583584 52-53 20.937338934585377 27.482048928584035 30.05669502594698 21.52391711088361 54-55 20.753023144861114 27.405000486977926 30.06629957470594 21.775676793455016 56-57 20.76824836831893 27.875251367084143 29.720656200388074 21.635844064208857 58-59 20.825407129350005 27.24266884931275 29.988004854505938 21.943919166831304 60-61 20.86291243175818 27.197249020379772 30.275309619256856 21.664528928605193 62-63 20.746436519169627 27.23344627155693 30.426855684141753 21.593261525131695 64-65 20.7080043123155 27.175207572516324 30.31758171668539 21.799206398482788 66-67 20.686331783943352 27.399367261869482 30.062398929820255 21.851902024366908 68-69 20.57966883323973 27.233343587630493 30.15581827880211 22.031169300327665 70-71 20.718793393467976 27.372317366418674 30.053257544113166 21.85563169600018 72-73 20.71446210726104 27.362078332592482 29.803074882345886 22.12038467780059 74-75 20.53709670817614 27.556163401377248 29.838549186818025 22.068190703628584 76-77 20.647711572467458 27.33863308245847 29.874242682973296 22.13941266210077 78-79 20.67320109236404 27.452491338004815 29.551975499431943 22.32233207019921 80-81 20.600591870460246 27.576026565020374 29.5146351755078 22.30874638901159 82-83 20.564834163211 27.40698621072073 29.559259473515638 22.46892015255263 84-85 20.49377281314771 27.102901317395343 29.663194341563347 22.740131527893592 86-87 20.579379278816578 27.30653964025488 29.603766769574612 22.510314311353927 88-89 20.638004848464323 27.04980259017494 29.4017875537268 22.910405007633933 90-91 20.370506256949813 27.14604629634856 29.77427566704109 22.70917177966054 92-93 20.570986696168024 27.06725857524257 29.655071204005264 22.706683524584147 94-95 20.288681207499675 27.01902575384064 29.510308732825248 23.18198430583444 96-97 20.30933145175516 27.0865408361263 29.34401128946904 23.260116422649496 98-99 20.34376958207961 27.145913661023265 29.281665810465228 23.228650946431898 100-101 20.55350803424785 26.59824245536999 29.687914481964565 23.160335028417588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 648.0 1 402.0 2 196.5 3 246.0 4 201.0 5 170.5 6 320.0 7 740.0 8 1204.5 9 1395.0 10 1191.0 11 921.5 12 890.0 13 934.0 14 989.5 15 997.0 16 1028.0 17 1080.5 18 1083.5 19 1155.5 20 1253.5 21 1358.0 22 1534.0 23 1738.5 24 1951.0 25 2237.0 26 2786.0 27 3400.0 28 3680.0 29 4068.0 30 4787.0 31 5484.5 32 6181.5 33 6861.5 34 7673.0 35 8392.5 36 9181.0 37 10065.5 38 10316.0 39 10300.5 40 10387.5 41 10710.5 42 11046.5 43 11640.0 44 12343.5 45 12668.0 46 12564.5 47 12567.0 48 13527.5 49 13388.0 50 12132.0 51 11426.5 52 10792.5 53 10439.0 54 10121.0 55 9262.5 56 8351.0 57 7578.0 58 6864.0 59 5987.5 60 4958.5 61 4047.5 62 3320.5 63 2623.0 64 1948.5 65 1489.0 66 1120.0 67 821.0 68 588.5 69 396.5 70 277.0 71 209.0 72 158.0 73 122.0 74 93.5 75 74.0 76 57.5 77 43.0 78 29.0 79 18.5 80 11.5 81 9.0 82 5.5 83 5.5 84 4.0 85 1.0 86 2.0 87 2.0 88 1.0 89 0.5 90 1.0 91 2.0 92 2.5 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3670815467571177 2 0.31242780917393975 3 0.3036944799725041 4 0.3146815715485038 5 0.31637189332942683 6 0.22453107656594226 7 0.21129022261537855 8 0.21495258647404508 9 0.22622139834686528 10-11 0.22368591567548074 12-13 0.21593860751291685 14-15 0.21946011122317316 16-17 0.2035429144528147 18-19 0.2111493624669683 20-21 0.19649990703230208 22-23 0.2057966768273787 24-25 0.20466979564009669 26-27 0.20734613845989147 28-29 0.22424935626912176 30-31 0.21946011122317316 32-33 0.22791172012778835 34-35 0.2205869924104552 36-37 0.1987536694068661 38-39 0.18551281545630235 40-41 0.18255475233968707 42-43 0.20086657163301988 44-45 0.20368377460122492 46-47 0.2041063550464557 48-49 0.22143215330091673 50-51 0.21875581048112192 52-53 0.24636439956953138 54-55 0.20748699860830172 56-57 0.18297733278491782 58-59 0.1836816335269691 60-61 0.17339884269302067 62-63 0.17987840951989226 64-65 0.17692034640327697 66-67 0.17720206670009747 68-69 0.18001926966830253 70-71 0.18199131174604605 72-73 0.18016012981671278 74-75 0.1987536694068661 76-77 0.18833001842450742 78-79 0.1928375431736355 80-81 0.18635797634676388 82-83 0.16691927586614905 84-85 0.16579239467886703 86-87 0.16903217809230284 88-89 0.17551174491917443 90-91 0.179174108777841 92-93 0.17692034640327697 94-95 0.1764977659580462 96-97 0.18297733278491782 98-99 0.19269668302522525 100-101 0.17015905927958486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 354962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.92928768900984 #Duplication Level Percentage of deduplicated Percentage of total 1 84.01043694363392 55.38748266136261 2 9.422488541184642 12.424359155563218 3 2.727309078234663 5.39428634507344 4 1.1429008310325734 3.0140255075661986 5 0.6262193978138272 2.0643099417453157 6 0.4583887446074209 1.8132746049976025 7 0.2959650136597504 1.3658933772018789 8 0.2092518433602487 1.1036659984282768 9 0.15608896157652602 0.9261750647571829 >10 0.8879695413493957 10.293614214916703 >50 0.04927093134636923 2.2569300248194972 >100 0.01156795779432228 1.3109402059021305 >500 8.568857625423911E-4 0.32851184215991763 >1k 0.0012853286438135867 2.3165310555060055 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3488 0.9826403953099204 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2822 0.7950146776274644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1891 0.5327330812875744 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 654 0.1842450741206101 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 0.14339563108163692 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 483 0.13607090336430377 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 422 0.11888596525825298 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.817202968205047E-4 0.0 0.0 0.0 0.0 6 2.817202968205047E-4 0.0 0.0 0.0 0.0 7 2.817202968205047E-4 0.0 0.0 0.0 0.0 8 2.817202968205047E-4 0.0 0.0 0.0 0.0 9 2.817202968205047E-4 0.0 0.0 0.0 0.0 10-11 2.817202968205047E-4 0.0 0.0 0.0 0.0 12-13 2.817202968205047E-4 0.0 0.0 0.0 0.0 14-15 2.817202968205047E-4 0.0 0.0 0.0 0.0 16-17 2.817202968205047E-4 0.0 0.0 0.0 0.0 18-19 2.817202968205047E-4 0.0 0.0 0.0 0.0 20-21 2.817202968205047E-4 0.0 0.0 0.0 0.0 22-23 2.817202968205047E-4 0.0 0.0 4.225804452307571E-4 0.0 24-25 2.817202968205047E-4 0.0 0.0 5.634405936410094E-4 0.0 26-27 2.817202968205047E-4 0.0 0.0 0.0011268811872820188 0.0 28-29 5.634405936410094E-4 0.0 0.0 0.003098923265025552 0.0 30-31 5.634405936410094E-4 0.0 0.0 0.0059161262332306 0.0 32-33 5.634405936410094E-4 0.0 0.0 0.010282790833948422 0.0 34-35 5.634405936410094E-4 0.0 0.0 0.012254832911691957 0.0 36-37 5.634405936410094E-4 0.0 0.0 0.014508595286255994 0.0 38-39 5.634405936410094E-4 0.0 0.0 0.02281934404246088 2.817202968205047E-4 40-41 5.634405936410094E-4 0.0 0.0 0.038595680664409146 2.817202968205047E-4 42-43 5.634405936410094E-4 0.0 0.0 0.0594429826291265 2.817202968205047E-4 44-45 5.634405936410094E-4 0.0 0.0 0.08564297023343344 2.817202968205047E-4 46-47 7.043007420512617E-4 0.0 0.0 0.13705692440317557 2.817202968205047E-4 48-49 8.451608904615142E-4 0.0 0.0 0.19354184391568674 2.817202968205047E-4 50-51 8.451608904615142E-4 0.0 0.0 0.23551816814194193 2.817202968205047E-4 52-53 8.451608904615142E-4 0.0 0.0 0.29143964706081216 2.817202968205047E-4 54-55 8.451608904615142E-4 0.0 0.0 0.34651596508922083 2.817202968205047E-4 56-57 0.0011268811872820188 0.0 0.0 0.4582180627785509 2.817202968205047E-4 58-59 0.0011268811872820188 0.0 0.0 0.5845696159025473 2.817202968205047E-4 60-61 0.0011268811872820188 0.0 0.0 0.7179641764470563 2.817202968205047E-4 62-63 0.0011268811872820188 0.0 0.0 0.8575565835216163 2.817202968205047E-4 64-65 0.0011268811872820188 0.0 0.0 1.0144747888506376 2.817202968205047E-4 66-67 0.0011268811872820188 0.0 0.0 1.2288639347310417 2.817202968205047E-4 68-69 0.0011268811872820188 0.0 0.0 1.5038229444278541 2.817202968205047E-4 70-71 0.0011268811872820188 0.0 0.0 1.7182120903082585 2.817202968205047E-4 72-73 0.0011268811872820188 0.0 0.0 1.9361227398989187 2.817202968205047E-4 74-75 0.0011268811872820188 0.0 0.0 2.18812154540486 2.817202968205047E-4 76-77 0.0011268811872820188 0.0 0.0 2.4615310934691603 2.817202968205047E-4 78-79 0.0011268811872820188 0.0 0.0 2.779311588282689 2.817202968205047E-4 80-81 0.0011268811872820188 0.0 0.0 3.169916779824319 2.817202968205047E-4 82-83 0.0011268811872820188 0.0 0.0 3.5782703500656408 2.817202968205047E-4 84-85 0.0012677413356922713 0.0 0.0 4.001414235890039 2.817202968205047E-4 86-87 0.0014086014841025237 0.0 0.0 4.434840912548386 2.817202968205047E-4 88-89 0.0014086014841025237 0.0 0.0 5.056175027186009 2.817202968205047E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTCCG 40 5.381563E-4 47.50275 7 GGTATCA 1470 0.0 47.26637 1 GTATCAA 2620 0.0 39.961163 1 ATCAACG 3330 0.0 31.137472 3 TATCAAC 3380 0.0 30.681194 2 TCAACGC 3405 0.0 30.455927 4 CAACGCA 3480 0.0 29.799551 5 GTCTAGG 80 4.4378842E-4 29.743818 1 CATTCCG 80 4.4859102E-4 29.689219 9 AACGCAG 3545 0.0 29.211847 6 GTACATG 2395 0.0 28.017561 1 TAAGGTG 170 3.5670382E-9 27.982307 5 ATATAGG 85 6.3961925E-4 27.942795 6 GTAAGGT 120 5.306809E-6 27.749125 4 TACATGG 2445 0.0 27.238401 2 ACATGGG 2435 0.0 26.955734 3 ACGCAGA 3880 0.0 26.56726 7 CGCAGAG 3870 0.0 26.513165 8 GTATAGG 110 9.337344E-5 25.958242 1 GCAGAGT 3930 0.0 25.262281 9 >>END_MODULE