FastQCFastQC Report
Thu 26 May 2016
SRR937742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354962
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37311.0510984274373032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.8843200117195643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.6172491703337258No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.20565581667896846No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.17579346521599495No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.13240853950563722No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA250.005010740457.0220685
GGTATCA18500.044.4361151
GTATCAA30900.036.5999261
CTGTCCG851.6294363E-533.5282179
CCGCTCC650.00583971829.2297299
TATCAAC39500.028.1501332
ATCAACG40150.027.6944073
TCAACGC40300.027.5913224
CAACGCA40650.027.2368645
GGGCGTA700.00838535127.1418919
AACGCAG41550.026.6356356
GTACATG24550.025.1624871
TACATGG25250.023.900342
ATATAGG1201.7030956E-423.7591934
GTACACT1601.8541032E-623.7491556
ACGCAGA46900.023.4959647
ACATGGG24950.023.2354453
CGCAGAG47500.022.9991828
TAAGGCA1452.3215069E-522.9399134
GAGTACT27550.022.84401312-13
Produced by FastQC (version 0.11.5)